Gi2 - NPS receptor complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gi/o

DATE

2023-09-15

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.


Tertiary structure confidence

(pLDDT score) avg.: 80.0

Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.7
iPTM Score: 0.6
PAE mean: 13.6


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM3 I 150 3x54 G.H5 L 354 G.H5.25
Polar ICL3 Y 260 - G.H5 A 339 G.H5.10
Hydrophobic ICL3 Y 260 - G.H5 A 339 G.H5.10
Van-der-waals ICL3 Y 260 - G.H5 A 339 G.H5.10
Hydrophobic ICL3 S 258 - G.S6 T 322 G.S6.03
Van-der-waals ICL3 S 258 - G.S6 T 322 G.S6.03
Hydrophobic TM4 K 161 4x40 G.HN R 24 G.HN.48
Van-der-waals TM4 K 161 4x40 G.HN R 24 G.HN.48
Hydrophobic ICL2 M 154 34x51 G.S3 L 195 G.S3.01
Van-der-waals ICL2 M 154 34x51 G.S3 L 195 G.S3.01
Hydrophobic TM6 I 265 6x26 G.H5 D 342 G.H5.13
Van-der-waals TM6 I 265 6x26 G.H5 D 342 G.H5.13
Hydrophobic TM5 K 240 5x68 G.H5 I 345 G.H5.16
Van-der-waals TM5 K 240 5x68 G.H5 I 345 G.H5.16
Polar TM5 Y 244 5x72 G.H5 A 339 G.H5.10
Hydrophobic ICL3 L 256 - G.H4 A 301 G.H4.07
Van-der-waals ICL3 L 256 - G.H4 A 301 G.H4.07
Hydrophobic ICL2 L 157 - G.hns1 A 31 G.hns1.02
Van-der-waals ICL2 L 157 - G.hns1 A 31 G.hns1.02
Hydrophobic TM3 I 150 3x54 G.H5 L 349 G.H5.20
Van-der-waals TM3 I 150 3x54 G.H5 L 349 G.H5.20
Hydrophobic ICL2 F 156 34x53 G.H5 C 352 G.H5.23
Polar TM3 R 146 3x50 G.H5 C 352 G.H5.23
Hydrophobic TM3 R 146 3x50 G.H5 C 352 G.H5.23
Van-der-waals TM3 R 146 3x50 G.H5 C 352 G.H5.23
Hydrophobic TM6 A 268 6x29 G.H5 F 355 G.H5.26
Van-der-waals TM6 A 268 6x29 G.H5 F 355 G.H5.26
Polar TM5 Y 244 5x72 G.H5 D 338 G.H5.09
Hydrophobic TM5 Y 244 5x72 G.H5 D 338 G.H5.09
Van-der-waals TM5 Y 244 5x72 G.H5 D 338 G.H5.09
Hydrophobic ICL2 M 154 34x51 G.H5 T 341 G.H5.12
Van-der-waals ICL2 M 154 34x51 G.H5 T 341 G.H5.12
Polar ICL3 S 259 - G.H4 Q 305 G.H4.12
Hydrophobic ICL3 S 259 - G.H4 Q 305 G.H4.12
Van-der-waals ICL3 S 259 - G.H4 Q 305 G.H4.12
Polar ICL3 Y 260 - G.S6 H 323 G.S6.04
Aromatic ICL3 Y 260 - G.S6 H 323 G.S6.04
Hydrophobic ICL3 Y 260 - G.S6 H 323 G.S6.04
Van-der-waals ICL3 Y 260 - G.S6 H 323 G.S6.04
Polar ICL3 Y 260 - G.S6 T 322 G.S6.03
Hydrophobic TM3 A 149 3x53 G.H5 C 352 G.H5.23
Van-der-waals TM3 A 149 3x53 G.H5 C 352 G.H5.23
Hydrophobic TM6 I 265 6x26 G.S6 Y 321 G.S6.02
Van-der-waals TM6 I 265 6x26 G.S6 Y 321 G.S6.02
Hydrophobic ICL3 S 258 - G.S6 F 324 G.S6.05
Hydrophobic ICL3 L 256 - G.H4 D 298 G.H4.04
Van-der-waals ICL3 L 256 - G.H4 D 298 G.H4.04
Hydrophobic TM2 M 83 2x39 G.H5 C 352 G.H5.23
Van-der-waals TM2 M 83 2x39 G.H5 C 352 G.H5.23
Hydrophobic ICL2 M 154 34x51 G.H5 I 344 G.H5.15
Polar TM3 A 149 3x53 G.H5 N 348 G.H5.19
Hydrophobic TM3 A 149 3x53 G.H5 N 348 G.H5.19
Van-der-waals TM3 A 149 3x53 G.H5 N 348 G.H5.19
Polar TM6 L 264 6x25 G.h4s6 E 319 G.h4s6.12
Hydrophobic TM6 L 264 6x25 G.h4s6 E 319 G.h4s6.12
Van-der-waals TM6 L 264 6x25 G.h4s6 E 319 G.h4s6.12
Polar TM6 K 271 6x32 G.H5 F 355 G.H5.26
Hydrophobic TM6 K 271 6x32 G.H5 F 355 G.H5.26
Van-der-waals TM6 K 271 6x32 G.H5 F 355 G.H5.26
Hydrophobic TM4 R 164 4x43 G.HN R 24 G.HN.48
Van-der-waals TM4 R 164 4x43 G.HN R 24 G.HN.48
Polar TM3 R 146 3x50 G.H5 G 353 G.H5.24
Hydrophobic TM6 L 264 6x25 G.h4s6 K 318 G.h4s6.11
Hydrophobic C-term L 365 - G.H2 K 210 G.H2.05
Van-der-waals C-term L 365 - G.H2 K 210 G.H2.05
Hydrophobic ICL2 F 156 34x53 G.H5 N 348 G.H5.19
Hydrophobic TM2 M 83 2x39 G.H5 G 353 G.H5.24
Van-der-waals TM2 M 83 2x39 G.H5 G 353 G.H5.24
Hydrophobic TM5 I 237 5x65 G.H5 L 349 G.H5.20
Hydrophobic TM6 L 264 6x25 G.h4s6 K 315 G.h4s6.08
Van-der-waals TM6 L 264 6x25 G.h4s6 K 315 G.h4s6.08
Hydrophobic TM6 L 264 6x25 G.h4s6 D 316 G.h4s6.09
Van-der-waals TM6 L 264 6x25 G.h4s6 D 316 G.h4s6.09
Polar ICL3 S 258 - G.S6 H 323 G.S6.04
Hydrophobic ICL3 S 258 - G.S6 H 323 G.S6.04
Van-der-waals ICL3 S 258 - G.S6 H 323 G.S6.04
Polar ICL2 Q 158 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 Q 158 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 Q 158 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 P 153 34x50 G.H5 I 345 G.H5.16
Hydrophobic ICL2 L 157 - G.S1 V 34 G.S1.02
Polar TM2 M 83 2x39 G.H5 D 351 G.H5.22
Hydrophobic TM2 M 83 2x39 G.H5 D 351 G.H5.22
Van-der-waals TM2 M 83 2x39 G.H5 D 351 G.H5.22
Hydrophobic ICL3 L 256 - G.S6 F 324 G.S6.05
Van-der-waals ICL3 L 256 - G.S6 F 324 G.S6.05
Polar TM6 I 265 6x26 G.h4s6 E 319 G.h4s6.12
Hydrophobic TM6 I 265 6x26 G.h4s6 E 319 G.h4s6.12
Van-der-waals TM6 I 265 6x26 G.h4s6 E 319 G.h4s6.12
Hydrophobic ICL2 L 157 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 L 157 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL3 C 257 - G.S6 F 324 G.S6.05
Van-der-waals ICL3 C 257 - G.S6 F 324 G.S6.05
Hydrophobic ICL2 M 154 34x51 G.s2s3 K 193 G.s2s3.01
Van-der-waals ICL2 M 154 34x51 G.s2s3 K 193 G.s2s3.01
Polar ICL2 P 153 34x50 G.H5 N 348 G.H5.19
Van-der-waals ICL2 P 153 34x50 G.H5 N 348 G.H5.19
Hydrophobic ICL3 Y 260 - G.S6 Y 321 G.S6.02
Van-der-waals ICL3 Y 260 - G.S6 Y 321 G.S6.02
Hydrophobic TM3 R 146 3x50 G.H5 L 354 G.H5.25
Hydrophobic TM5 V 247 5x75 G.H5 F 335 G.H5.06
Van-der-waals TM5 V 247 5x75 G.H5 F 335 G.H5.06
Polar TM6 A 272 6x33 G.H5 L 354 G.H5.25
Hydrophobic TM6 A 272 6x33 G.H5 L 354 G.H5.25
Van-der-waals TM6 A 272 6x33 G.H5 L 354 G.H5.25
Polar TM2 S 81 2x37 G.H5 D 351 G.H5.22
Hydrophobic TM2 S 81 2x37 G.H5 D 351 G.H5.22
Van-der-waals TM2 S 81 2x37 G.H5 D 351 G.H5.22
Hydrophobic ICL3 L 256 - G.H4 Y 297 G.H4.03
Van-der-waals ICL3 L 256 - G.H4 Y 297 G.H4.03
Hydrophobic TM6 Y 275 6x36 G.H5 L 354 G.H5.25
Polar ICL3 N 261 - G.H4 E 309 G.H4.16
Hydrophobic ICL3 N 261 - G.H4 E 309 G.H4.16
Van-der-waals ICL3 N 261 - G.H4 E 309 G.H4.16
Polar TM5 Y 244 5x72 G.H5 D 342 G.H5.13
Hydrophobic TM5 Y 244 5x72 G.H5 D 342 G.H5.13
Van-der-waals TM5 Y 244 5x72 G.H5 D 342 G.H5.13
Ionic ICL2 K 155 34x52 G.s2s3 D 194 G.s2s3.02
Polar ICL2 K 155 34x52 G.s2s3 D 194 G.s2s3.02
Hydrophobic ICL2 K 155 34x52 G.s2s3 D 194 G.s2s3.02
Van-der-waals ICL2 K 155 34x52 G.s2s3 D 194 G.s2s3.02
Hydrophobic ICL2 P 153 34x50 G.H5 I 344 G.H5.15
Hydrophobic ICL2 L 157 - G.S3 L 195 G.S3.01
Van-der-waals ICL2 L 157 - G.S3 L 195 G.S3.01
Ionic TM4 K 161 4x40 G.HN E 28 G.HN.52
Polar TM4 K 161 4x40 G.HN E 28 G.HN.52
Hydrophobic TM4 K 161 4x40 G.HN E 28 G.HN.52
Van-der-waals TM4 K 161 4x40 G.HN E 28 G.HN.52
Van-der-waals C-term R 359 - G.H3 K 258 G.H3.16
Hydrophobic TM6 A 272 6x33 G.H5 L 349 G.H5.20