G12 - EP4 receptor complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

G12/13

DATE

2023-09-15

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.


Tertiary structure confidence

(pLDDT score) avg.: 79.5

Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.7
iPTM Score: 0.7
PAE mean: 14.0


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL3 F 260 - G.h4s6 P 345 G.h4s6.20
Hydrophobic ICL2 A 124 34x50 G.H5 I 370 G.H5.15
Hydrophobic TM5 F 217 5x72 G.H5 Q 372 G.H5.17
Van-der-waals TM5 F 217 5x72 G.H5 Q 372 G.H5.17
Polar TM3 A 120 3x53 G.H5 N 374 G.H5.19
Hydrophobic TM3 A 120 3x53 G.H5 N 374 G.H5.19
Van-der-waals TM3 A 120 3x53 G.H5 N 374 G.H5.19
Ionic ICL3 R 256 - G.H4 D 336 G.H4.16
Polar ICL3 R 256 - G.H4 D 336 G.H4.16
Hydrophobic ICL3 R 256 - G.H4 D 336 G.H4.16
Van-der-waals ICL3 R 256 - G.H4 D 336 G.H4.16
Van-der-waals ICL2 Y 125 34x51 G.H5 V 366 G.H5.11
Polar ICL2 H 129 34x55 G.hns1 R 56 G.hns1.03
Aromatic ICL2 H 129 34x55 G.hns1 R 56 G.hns1.03
Hydrophobic ICL2 H 129 34x55 G.hns1 R 56 G.hns1.03
Van-der-waals ICL2 H 129 34x55 G.hns1 R 56 G.hns1.03
Polar TM6 Q 269 6x32 G.H5 Q 381 G.H5.26
Hydrophobic TM6 M 270 6x33 G.H5 I 378 G.H5.23
Van-der-waals TM6 M 270 6x33 G.H5 I 378 G.H5.23
Hydrophobic TM6 M 270 6x33 G.H5 M 379 G.H5.24
Hydrophobic ICL2 Y 125 34x51 G.H5 I 370 G.H5.15
Van-der-waals ICL2 Y 125 34x51 G.H5 I 370 G.H5.15
Hydrophobic TM5 L 210 5x65 G.H5 L 375 G.H5.20
Hydrophobic ICL3 F 260 - G.h4s6 R 342 G.h4s6.11
Van-der-waals ICL3 F 260 - G.h4s6 R 342 G.h4s6.11
Hydrophobic ICL3 R 256 - G.h4s6 R 342 G.h4s6.11
Van-der-waals ICL3 R 256 - G.h4s6 R 342 G.h4s6.11
Polar ICL1 Q 49 - G.H5 L 380 G.H5.25
Hydrophobic ICL1 Q 49 - G.H5 L 380 G.H5.25
Van-der-waals ICL1 Q 49 - G.H5 L 380 G.H5.25
Hydrophobic TM5 T 221 5x76 G.h4s6 P 345 G.h4s6.20
Hydrophobic TM2 T 52 2x37 G.H5 D 377 G.H5.22
Hydrophobic TM6 A 266 6x29 G.H5 M 379 G.H5.24
Van-der-waals TM6 A 266 6x29 G.H5 M 379 G.H5.24
Polar H8 R 333 8x47 G.H5 I 378 G.H5.23
Van-der-waals H8 R 333 8x47 G.H5 I 378 G.H5.23
Polar ICL3 S 259 - G.h4s6 R 342 G.h4s6.11
Polar H8 T 335 8x49 G.H5 L 380 G.H5.25
Polar ICL2 Y 125 34x51 G.H5 F 363 G.H5.08
Aromatic ICL2 Y 125 34x51 G.H5 F 363 G.H5.08
Hydrophobic ICL2 Y 125 34x51 G.H5 F 363 G.H5.08
Van-der-waals ICL2 Y 125 34x51 G.H5 F 363 G.H5.08
Hydrophobic TM6 E 267 6x30 G.H5 L 375 G.H5.20
Hydrophobic TM6 I 263 6x26 G.h4s6 S 343 G.h4s6.12
Van-der-waals TM6 I 263 6x26 G.h4s6 S 343 G.h4s6.12
Polar H8 R 333 8x47 G.H5 M 379 G.H5.24
Hydrophobic H8 R 333 8x47 G.H5 M 379 G.H5.24
Van-der-waals H8 R 333 8x47 G.H5 M 379 G.H5.24
Hydrophobic TM2 Y 55 2x40 G.H5 L 380 G.H5.25
Hydrophobic TM3 I 121 3x54 G.H5 I 378 G.H5.23
Hydrophobic ICL2 Y 125 34x51 G.S1 V 58 G.S1.02
Hydrophobic ICL2 Y 127 34x53 G.H5 N 374 G.H5.19
Hydrophobic TM3 I 121 3x54 G.H5 L 371 G.H5.16
Van-der-waals TM3 I 121 3x54 G.H5 L 371 G.H5.16
Ionic TM4 D 132 4x38 G.hns1 R 55 G.hns1.02
Polar TM4 D 132 4x38 G.hns1 R 55 G.hns1.02
Hydrophobic TM4 D 132 4x38 G.hns1 R 55 G.hns1.02
Van-der-waals TM4 D 132 4x38 G.hns1 R 55 G.hns1.02
Hydrophobic TM1 L 42 1x57 G.H5 L 380 G.H5.25
Ionic C-term E 384 - G.s4h3 R 266 G.s4h3.13
Polar C-term E 384 - G.s4h3 R 266 G.s4h3.13
Hydrophobic C-term E 384 - G.s4h3 R 266 G.s4h3.13
Van-der-waals C-term E 384 - G.s4h3 R 266 G.s4h3.13
Hydrophobic H8 R 333 8x47 G.H5 L 380 G.H5.25
Van-der-waals H8 R 333 8x47 G.H5 L 380 G.H5.25
Hydrophobic TM6 Q 269 6x32 G.H5 M 379 G.H5.24
Van-der-waals TM6 Q 269 6x32 G.H5 M 379 G.H5.24
Hydrophobic ICL2 A 124 34x50 G.H5 L 371 G.H5.16
Hydrophobic TM5 T 221 5x76 G.S6 H 348 G.S6.03
Hydrophobic TM5 F 217 5x72 G.h4s6 P 345 G.h4s6.20
Van-der-waals TM5 F 217 5x72 G.h4s6 P 345 G.h4s6.20
Aromatic ICL2 Y 130 34x56 G.hns1 R 56 G.hns1.03
Hydrophobic ICL3 S 222 - G.S6 H 348 G.S6.03
Ionic TM5 R 220 5x75 G.H5 D 368 G.H5.13
Polar TM5 R 220 5x75 G.H5 D 368 G.H5.13
Van-der-waals TM5 R 220 5x75 G.H5 D 368 G.H5.13
Hydrophobic TM3 I 121 3x54 G.H5 L 375 G.H5.20
Hydrophobic ICL2 Y 125 34x51 G.S3 I 221 G.S3.01
Van-der-waals ICL2 Y 125 34x51 G.S3 I 221 G.S3.01
Hydrophobic TM6 A 266 6x29 G.H5 L 375 G.H5.20
Polar ICL2 A 124 34x50 G.H5 N 374 G.H5.19
Hydrophobic TM3 A 120 3x53 G.H5 I 378 G.H5.23
Van-der-waals TM3 A 120 3x53 G.H5 I 378 G.H5.23
Hydrophobic TM5 M 213 5x68 G.H5 Q 372 G.H5.17
Van-der-waals TM5 M 213 5x68 G.H5 Q 372 G.H5.17
Hydrophobic TM6 I 263 6x26 G.h4s6 K 344 G.h4s6.19
Van-der-waals TM6 I 263 6x26 G.h4s6 K 344 G.h4s6.19
Polar ICL1 Q 49 - G.H5 Q 381 G.H5.26
Hydrophobic ICL1 Q 49 - G.H5 Q 381 G.H5.26
Van-der-waals ICL1 Q 49 - G.H5 Q 381 G.H5.26
Polar C-term S 371 - G.H3 K 284 G.H3.16
Van-der-waals C-term S 371 - G.H3 K 284 G.H3.16
Polar ICL2 Y 125 34x51 G.H5 K 367 G.H5.12
Hydrophobic ICL2 Y 125 34x51 G.H5 K 367 G.H5.12
Hydrophobic TM5 M 213 5x68 G.H5 L 371 G.H5.16
Van-der-waals TM5 M 213 5x68 G.H5 L 371 G.H5.16
Hydrophobic TM6 M 270 6x33 G.H5 L 375 G.H5.20
Van-der-waals TM6 M 270 6x33 G.H5 L 375 G.H5.20
Hydrophobic TM2 F 54 2x39 G.H5 I 378 G.H5.23
Van-der-waals TM2 F 54 2x39 G.H5 I 378 G.H5.23
Hydrophobic TM2 F 54 2x39 G.H5 L 380 G.H5.25
Polar TM5 R 220 5x75 G.H5 H 364 G.H5.09
Aromatic TM5 R 220 5x75 G.H5 H 364 G.H5.09
Hydrophobic TM5 R 220 5x75 G.H5 H 364 G.H5.09
Van-der-waals TM5 R 220 5x75 G.H5 H 364 G.H5.09
Hydrophobic ICL3 F 260 - G.h4s6 K 344 G.h4s6.19
Van-der-waals ICL3 F 260 - G.h4s6 K 344 G.h4s6.19
Hydrophobic TM6 I 263 6x26 G.h4s6 P 345 G.h4s6.20
Hydrophobic H8 T 335 8x49 G.H5 Q 381 G.H5.26
Hydrophobic TM3 R 117 3x50 G.H5 I 378 G.H5.23
Hydrophobic TM5 T 221 5x76 G.S6 F 347 G.S6.02