ptafr_human
NAME
5ZKP
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
ptafr_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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E
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P
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H
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D
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S
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S
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H
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M
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D
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10 |
TM1 | |||||||||
S
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E
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F
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R
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Y
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T
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L
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F
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P
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I
|
20 |
V
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Y
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S
|
I
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I
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F
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V
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L
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G
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V
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30 |
I
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A
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N
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G
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Y
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V
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L
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W
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V
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F
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40 |
ICL1 | |||||||||
A
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R
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L
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Y
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P
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C
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K
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K
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F
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N
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50 |
TM2 | |||||||||
E
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I
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K
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I
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F
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M
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V
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N
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L
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T
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60 |
M
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A
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D
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M
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L
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F
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L
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I
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T
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L
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70 |
P
|
L
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W
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I
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V
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Y
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Y
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Q
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N
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Q
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80 |
ECL1 | TM3 | ||||||||
G
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N
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W
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I
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L
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P
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K
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F
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L
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C
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90 |
N
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V
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A
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G
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C
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L
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F
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F
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I
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N
|
100 |
T
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Y
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C
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S
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V
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A
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F
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L
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G
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V
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110 |
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|||||||||
I
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T
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Y
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N
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R
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F
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Q
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A
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V
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T
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120 |
ICL2 | |||||||||
R
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P
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I
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K
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T
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A
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Q
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A
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N
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T
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130 |
TM4 | |||||||||
R
|
K
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R
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G
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I
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S
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L
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S
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L
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V
|
140 |
I
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W
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V
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A
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I
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V
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G
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A
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A
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S
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150 |
ECL2 | |||||||||
Y
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F
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L
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I
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L
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D
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S
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T
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N
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T
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160 |
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|||||||||
V
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P
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D
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S
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A
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G
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S
|
G
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N
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V
|
170 |
T
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R
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C
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F
|
E
|
H
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Y
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E
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K
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G
|
180 |
TM5 | |||||||||
S
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V
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P
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V
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L
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I
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I
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H
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I
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F
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190 |
I
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V
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F
|
S
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F
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F
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L
|
V
|
F
|
L
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200 |
I
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I
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L
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F
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C
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N
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L
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V
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I
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I
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210 |
|
|
|
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||||||
ICL3 | |||||||||
R
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T
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L
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L
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M
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Q
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P
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V
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Q
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Q
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220 |
|
|
|
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||||||
TM6 | |||||||||
Q
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R
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N
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A
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E
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V
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K
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R
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R
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A
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230 |
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|||||||||
L
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W
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M
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V
|
C
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T
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V
|
L
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A
|
V
|
240 |
F
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I
|
I
|
C
|
F
|
V
|
P
|
H
|
H
|
V
|
250 |
V
|
Q
|
L
|
P
|
W
|
T
|
L
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A
|
E
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L
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260 |
ECL3 | TM7 | ||||||||
G
|
F
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Q
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D
|
S
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K
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F
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H
|
Q
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A
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270 |
I
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N
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D
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A
|
H
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Q
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V
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T
|
L
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C
|
280 |
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|||||||||
L
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L
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S
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T
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N
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C
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V
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L
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D
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P
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290 |
V
|
I
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Y
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C
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F
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L
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T
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K
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K
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F
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300 |
H8 | |||||||||
R
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K
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H
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L
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T
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E
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K
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F
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Y
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S
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310 |
|
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|||||
C-term | |||||||||
M
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R
|
S
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S
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R
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K
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C
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S
|
R
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A
|
320 |
|
|
|
|
|
|
|
|
|
|
T
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T
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D
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T
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V
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T
|
E
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V
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V
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V
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330 |
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|
|
|
|
|
|
|
|
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P
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F
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N
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Q
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I
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P
|
G
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N
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S
|
L
|
340 |
|
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K
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N
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Expression | ||||||||||
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|||||||||
N-term | |||||||||
|
E
|
P
|
H
|
D
|
S
|
S
|
H
|
M
|
D
|
10 |
TM1 | |||||||||
S
|
E
|
F
|
R
|
Y
|
T
|
L
|
F
|
P
|
I
|
20 |
V
|
Y
|
S
|
I
|
I
|
F
|
V
|
L
|
G
|
V
|
30 |
I
|
A
|
N
|
G
|
Y
|
V
|
L
|
W
|
V
|
F
|
40 |
ICL1 | |||||||||
A
|
R
|
L
|
Y
|
P
|
C
|
K
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K
|
F
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N
|
50 |
TM2 | |||||||||
E
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I
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K
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I
|
F
|
M
|
V
|
N
|
L
|
T
|
60 |
M
|
A
|
D
|
M
|
L
|
F
|
L
|
I
|
T
|
L
|
70 |
P
|
L
|
W
|
I
|
V
|
Y
|
Y
|
Q
|
N
|
Q
|
80 |
ECL1 | TM3 | ||||||||
G
|
N
|
W
|
I
|
L
|
P
|
K
|
F
|
L
|
C
|
90 |
N
|
V
|
A
|
G
|
C
|
L
|
F
|
F
|
I
|
N
|
100 |
T
|
Y
|
C
|
S
|
V
|
A
|
F
|
L
|
G
|
V
|
110 |
|
|||||||||
I
|
T
|
Y
|
N
|
R
|
Y
|
Q
|
A
|
V
|
T
|
120 |
ICL2 | |||||||||
R
|
P
|
I
|
K
|
T
|
A
|
Q
|
A
|
N
|
T
|
130 |
TM4 | |||||||||
R
|
K
|
R
|
G
|
I
|
S
|
L
|
S
|
L
|
V
|
140 |
I
|
W
|
V
|
A
|
I
|
V
|
G
|
A
|
A
|
S
|
150 |
ECL2 | |||||||||
Y
|
F
|
L
|
I
|
L
|
D
|
S
|
T
|
N
|
T
|
160 |
|
|||||||||
V
|
P
|
D
|
S
|
A
|
G
|
S
|
G
|
D
|
V
|
170 |
T
|
R
|
C
|
F
|
E
|
H
|
Y
|
E
|
K
|
G
|
180 |
TM5 | |||||||||
S
|
V
|
P
|
V
|
L
|
I
|
I
|
H
|
I
|
F
|
190 |
I
|
V
|
F
|
S
|
F
|
F
|
L
|
V
|
F
|
L
|
200 |
I
|
I
|
L
|
F
|
C
|
N
|
L
|
V
|
I
|
I
|
210 |
|
||||||
R
|
T
|
L
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L
|
M
|
Q
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|
|
|
|
|
|
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Flavodoxin | |||||||||
|
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|
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|
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|||||||
TM6 | |||||||
A
|
E
|
V
|
K
|
R
|
R
|
D
|
L
|
231 |
|
|||||||||
W
|
M
|
A
|
C
|
T
|
V
|
L
|
A
|
V
|
F
|
241 |
I
|
I
|
C
|
F
|
V
|
P
|
H
|
H
|
V
|
V
|
251 |
Q
|
L
|
P
|
W
|
T
|
L
|
A
|
E
|
L
|
G
|
261 |
ECL3 | TM7 | ||||||||
F
|
Q
|
D
|
S
|
K
|
F
|
H
|
Q
|
A
|
I
|
271 |
N
|
D
|
A
|
H
|
Q
|
V
|
T
|
L
|
C
|
L
|
281 |
|
|||||||||
L
|
S
|
T
|
N
|
C
|
V
|
L
|
N
|
P
|
V
|
291 |
H8 | |||||||||
I
|
Y
|
C
|
F
|
L
|
T
|
K
|
K
|
F
|
R
|
301 |
K
|
H
|
L
|
T
|
E
|
K
|
F
|
Y
|
S
|
M
|
311 |
C-term | |||||||
R
|
S
|
S
|
R
|
||||
315 |
|
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K
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
116 | | F | Y |
169 | | N | D |
230 | | A | D |
234 | | V | A |
289 | | D | N |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: Flavodoxin
Sequence: None
Deletions:
Positions: Within Receptor_1 217:217
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: Flavodoxin
Sequence: None
Deletions:
Positions: Within Receptor_2 316:316
Presence in Crystal:YES
Remarks: None
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: Within Receptor_3 316:316
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|