GPCRdb

fpr2_human

NAME

6LW5

ORGANISM

Human (Homo sapiens)

PROTEIN NAME

fpr2_human

SOURCE

SWISSPROT

SEQUENCE

Perspective: Wild-type Construct


N-term
M	E	T	N	F	S	T	P	L	N
10



E	Y	E	E	V	S	Y	E	S	A
20


TM1
G	Y	T	V	L	R	I	L	P	L
30



V	V	L	G	V	T	F	V	L	G
40



V	L	G	N	G	L	V	I	W	V
50


					ICL1				TM2
A	G	F	R	M	T	R	T	V	T
60



T	I	C	Y	L	N	L	A	L	A
70



D	F	S	F	T	A	T	L	P	F
80



L	I	V	S	M	A	M	G	E	K
90


ECL1				TM3
W	P	F	G	W	F	L	C	K	L
100



I	H	I	V	V	D	I	N	L	F
110



G	S	V	F	L	I	G	F	I	A
120



L	D	R	C	I	C	V	L	H	P
130


ICL2							TM4
V	W	A	Q	N	H	R	T	V	S
140



L	A	M	K	V	I	V	G	P	W
150



I	L	A	L	V	L	T	L	P	V
160


				ECL2
F	L	F	L	T	T	V	T	I	P
170



N	G	D	T	Y	C	T	F	N	F
180


					TM5
A	S	W	G	G	T	P	E	E	R
190



L	K	V	A	I	T	M	L	T	A
200



R	G	I	I	R	F	V	I	G	F
210



S	L	P	M	S	I	V	A	I	C
220



Y	G	L	I	A	A	K	I	H	K
230


		ICL3				TM6
K	G	M	I	K	S	S	R	P	L
240



R	V	L	T	A	V	V	A	S	F
250



F	I	C	W	F	P	F	Q	L	V
260



A	L	L	G	T	V	W	L	K	E
270


ECL3						TM7
M	L	F	Y	G	K	Y	K	I	I
280



D	I	L	V	N	P	T	S	S	L
290



A	F	F	N	S	C	L	N	P	M
300


					H8
L	Y	V	F	V	G	Q	D	F	R
310



E	R	L	I	H	S	L	P	T	S
320


C-term
L	E	R	A	L	S	E	D	S	A
330



P	T	N	D	T	A	A	N	S	A
340



S	P	P	A	E	T	E	L	Q	A
350

M


Expression


BRIL (b562R


	N-term
	T	N	F	S	T	P	L	N	E
11


							TM1
Y	E	E	V	S	Y	E	S	A	G
21



Y	T	V	L	R	I	L	P	L	V
31



V	L	G	V	T	F	V	L	G	V
41



L	G	N	G	L	V	I	W	V	A
51


				ICL1				TM2
G	F	R	M	T	R	T	V	T	T
61



I	C	Y	L	N	L	A	L	A	D
71



F	S	F	T	A	T	L	P	F	L
81



I	V	S	M	A	M	G	E	K	W
91


ECL1				TM3
P	F	G	W	F	L	C	K	L	I
101



H	I	V	V	D	I	N	L	F	G
111



S	V	F	L	I	G	F	I	A	L
121



D	R	C	I	C	V	L	H	P	V
131


ICL2						TM4
W	A	Q	N	H	R	T	V	S	L
141



A	M	K	V	I	V	G	P	W	I
151



L	A	L	V	L	T	L	P	V	F
161


			ECL2
L	F	L	T	T	V	T	I	P	N
171



G	D	T	Y	C	T	F	N	F	A
181


				TM5
S	W	G	G	T	P	E	E	R	L
191



K	V	A	I	T	M	L	T	A	R
201



G	I	I	R	F	V	I	G	F	S
211



L	P	M	S	I	V	A	I	C	Y
221



G	L	I	A	A	K	I	H	K	K
231


	ICL3				TM6
G	M	I	K	S	S	R	P	L	R
241



V	L	T	A	V	V	A	S	F	F
251



I	C	W	F	P	F	Q	L	V	A
261


						ECL3
L	L	G	T	V	W	L	K	E	M
271


					TM7
L	F	Y	G	K	Y	K	I	I	D
281



I	L	V	N	P	T	S	S	L	A
291



F	F	N	S	C	L	N	P	M	L
301


				H8
Y	V	F	V	G	Q	D	F	R	E
311



R	L	I	H	S	L	P	T	S	L
321


C-term
E

SCHEMATIC

Perspective: Wild-type Construct

N-term

TM1

ICL1

TM2

ECL1

TM3

ICL2

TM4

ECL2

TM5

ICL3

TM6

ECL3

TM7

H8

C-term

Expres

BRIL (

N-term

TM1

ICL1

TM2

ECL1

TM3

ICL2

TM4

ECL2

TM5

ICL3

TM6

ECL3

TM7

H8

C-term

DIAGRAMS

MUTATIONS

Position	Wild-type	Mutated

DELETIONS

from 1 to 2

from 323 to 351

MODIFICATIONS

AUXILIARY PROTEINS

Protein_type: auto

Name: Expression tag

Sequence: None

Deletions:

Positions: N-term_0 None:None

Presence in Crystal:YES

Remarks: None

Protein_type: fusion

Name: BRIL (b562RIL)

Sequence: None

Deletions:

Positions: N-term_1 None:None

Presence in Crystal:YES

Remarks: None

EXPRESSION

Expression method	Host cell type	Host cell	Remarks

SOLUBILIZATION

Solvent type	Item	Concentration

Remarks:

PURIFICATION

Purification type	Description

Remarks:

CRYSTALLIZATION

METHOD:

TYPE: ()

PROTEIN CONCENTRATION:

Temperature:

ph : -

Remarks:

Chemical lists

Type	Item	Concentration