mc4r_human
NAME
7F53
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
mc4r_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
V
|
N
|
S
|
T
|
H
|
R
|
G
|
M
|
H
|
10 |
T
|
S
|
L
|
H
|
L
|
W
|
N
|
R
|
S
|
S
|
20 |
Y
|
R
|
L
|
H
|
S
|
N
|
A
|
S
|
E
|
S
|
30 |
L
|
G
|
K
|
G
|
Y
|
S
|
D
|
G
|
G
|
C
|
40 |
TM1 | |||||||||
Y
|
E
|
Q
|
L
|
F
|
V
|
S
|
P
|
E
|
V
|
50 |
F
|
V
|
T
|
L
|
G
|
V
|
I
|
S
|
L
|
L
|
60 |
E
|
N
|
I
|
L
|
V
|
I
|
V
|
A
|
I
|
A
|
70 |
ICL1 | TM2 | ||||||||
K
|
N
|
K
|
N
|
L
|
H
|
S
|
P
|
M
|
Y
|
80 |
F
|
F
|
I
|
C
|
S
|
L
|
A
|
V
|
A
|
D
|
90 |
M
|
L
|
V
|
S
|
V
|
S
|
N
|
G
|
S
|
E
|
100 |
T
|
I
|
V
|
I
|
T
|
L
|
L
|
N
|
S
|
T
|
110 |
ECL1 | TM3 | ||||||||
D
|
T
|
D
|
A
|
Q
|
S
|
F
|
T
|
V
|
N
|
120 |
I
|
D
|
N
|
V
|
I
|
D
|
S
|
V
|
I
|
C
|
130 |
S
|
S
|
L
|
L
|
A
|
S
|
I
|
C
|
S
|
L
|
140 |
L
|
S
|
I
|
A
|
V
|
D
|
R
|
Y
|
F
|
T
|
150 |
ICL2 | |||||||||
I
|
F
|
Y
|
A
|
L
|
Q
|
Y
|
H
|
N
|
I
|
160 |
TM4 | |||||||||
M
|
T
|
V
|
K
|
R
|
V
|
G
|
I
|
I
|
I
|
170 |
S
|
C
|
I
|
W
|
A
|
A
|
C
|
T
|
V
|
S
|
180 |
G
|
I
|
L
|
F
|
I
|
I
|
Y
|
S
|
D
|
S
|
190 |
TM5 | |||||||||
S
|
A
|
V
|
I
|
I
|
C
|
L
|
I
|
T
|
M
|
200 |
F
|
F
|
T
|
M
|
L
|
A
|
L
|
M
|
A
|
S
|
210 |
L
|
Y
|
V
|
H
|
M
|
F
|
L
|
M
|
A
|
R
|
220 |
L
|
H
|
I
|
K
|
R
|
I
|
A
|
V
|
L
|
P
|
230 |
ICL3 | TM6 | ||||||||
G
|
T
|
G
|
A
|
I
|
R
|
Q
|
G
|
A
|
N
|
240 |
M
|
K
|
G
|
A
|
I
|
T
|
L
|
T
|
I
|
L
|
250 |
I
|
G
|
V
|
F
|
V
|
V
|
C
|
W
|
A
|
P
|
260 |
F
|
F
|
L
|
H
|
L
|
I
|
F
|
Y
|
I
|
S
|
270 |
ECL3 | |||||||||
C
|
P
|
Q
|
N
|
P
|
Y
|
C
|
V
|
C
|
F
|
280 |
TM7 | |||||||||
M
|
S
|
H
|
F
|
N
|
L
|
Y
|
L
|
I
|
L
|
290 |
I
|
M
|
C
|
N
|
S
|
I
|
I
|
D
|
P
|
L
|
300 |
H8 | |||||||||
I
|
Y
|
A
|
L
|
R
|
S
|
Q
|
E
|
L
|
R
|
310 |
K
|
T
|
F
|
K
|
E
|
I
|
I
|
C
|
C
|
Y
|
320 |
C-term | |||||||||
P
|
L
|
G
|
G
|
L
|
C
|
D
|
L
|
S
|
S
|
330 |
R
|
Y
|
N-term | |||||||||
M
|
V
|
N
|
S
|
T
|
H
|
R
|
G
|
M
|
H
|
10 |
T
|
S
|
L
|
H
|
L
|
W
|
N
|
R
|
S
|
S
|
20 |
Y
|
R
|
L
|
H
|
S
|
N
|
A
|
S
|
E
|
S
|
30 |
L
|
G
|
K
|
G
|
Y
|
S
|
D
|
G
|
G
|
C
|
40 |
TM1 | |||||||||
Y
|
E
|
Q
|
L
|
F
|
V
|
S
|
P
|
E
|
V
|
50 |
F
|
V
|
T
|
L
|
G
|
V
|
I
|
S
|
L
|
L
|
60 |
E
|
N
|
I
|
L
|
V
|
I
|
V
|
A
|
I
|
A
|
70 |
ICL1 | TM2 | ||||||||
K
|
N
|
K
|
N
|
L
|
H
|
S
|
P
|
M
|
Y
|
80 |
F
|
F
|
I
|
C
|
S
|
L
|
A
|
V
|
A
|
D
|
90 |
M
|
L
|
V
|
S
|
V
|
S
|
N
|
G
|
S
|
E
|
100 |
T
|
I
|
V
|
I
|
T
|
L
|
L
|
N
|
S
|
T
|
110 |
ECL1 | TM3 | ||||||||
D
|
T
|
D
|
A
|
Q
|
S
|
F
|
T
|
V
|
N
|
120 |
I
|
D
|
N
|
V
|
I
|
D
|
S
|
V
|
I
|
C
|
130 |
S
|
S
|
L
|
L
|
A
|
S
|
I
|
C
|
S
|
L
|
140 |
L
|
S
|
I
|
A
|
V
|
D
|
R
|
Y
|
F
|
T
|
150 |
ICL2 | |||||||||
I
|
F
|
Y
|
A
|
L
|
Q
|
Y
|
H
|
N
|
I
|
160 |
TM4 | |||||||||
M
|
T
|
V
|
K
|
R
|
V
|
G
|
I
|
I
|
I
|
170 |
S
|
C
|
I
|
W
|
A
|
A
|
C
|
T
|
V
|
S
|
180 |
G
|
I
|
L
|
F
|
I
|
I
|
Y
|
S
|
D
|
S
|
190 |
TM5 | |||||||||
S
|
A
|
V
|
I
|
I
|
C
|
L
|
I
|
T
|
M
|
200 |
F
|
F
|
T
|
M
|
L
|
A
|
L
|
M
|
A
|
S
|
210 |
L
|
Y
|
V
|
H
|
M
|
F
|
L
|
M
|
A
|
R
|
220 |
L
|
H
|
I
|
K
|
R
|
I
|
A
|
V
|
L
|
P
|
230 |
ICL3 | TM6 | ||||||||
G
|
T
|
G
|
A
|
I
|
R
|
Q
|
G
|
A
|
N
|
240 |
M
|
K
|
G
|
A
|
I
|
T
|
L
|
T
|
I
|
L
|
250 |
I
|
G
|
V
|
F
|
V
|
V
|
C
|
W
|
A
|
P
|
260 |
F
|
F
|
L
|
H
|
L
|
I
|
F
|
Y
|
I
|
S
|
270 |
ECL3 | |||||||||
C
|
P
|
Q
|
N
|
P
|
Y
|
C
|
V
|
C
|
F
|
280 |
TM7 | |||||||||
M
|
S
|
H
|
F
|
N
|
L
|
Y
|
L
|
I
|
L
|
290 |
I
|
M
|
C
|
N
|
S
|
I
|
I
|
D
|
P
|
L
|
300 |
H8 | |||||||||
I
|
Y
|
A
|
L
|
R
|
S
|
Q
|
E
|
L
|
R
|
310 |
K
|
T
|
F
|
K
|
E
|
I
|
I
|
C
|
C
|
Y
|
320 |
C-term | |||||||||
P
|
L
|
G
|
G
|
L
|
C
|
D
|
L
|
S
|
S
|
330 |
R
|
Y
|
332 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|