fzd4_human
NAME
8WMA
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
fzd4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
A
|
W
|
R
|
G
|
A
|
G
|
P
|
S
|
V
|
10 |
P
|
G
|
A
|
P
|
G
|
G
|
V
|
G
|
L
|
S
|
20 |
L
|
G
|
L
|
L
|
L
|
Q
|
L
|
L
|
L
|
L
|
30 |
L
|
G
|
P
|
A
|
R
|
G
|
F
|
G
|
D
|
E
|
40 |
E
|
E
|
R
|
R
|
C
|
D
|
P
|
I
|
R
|
I
|
50 |
S
|
M
|
C
|
Q
|
N
|
L
|
G
|
Y
|
N
|
V
|
60 |
T
|
K
|
M
|
P
|
N
|
L
|
V
|
G
|
H
|
E
|
70 |
L
|
Q
|
T
|
D
|
A
|
E
|
L
|
Q
|
L
|
T
|
80 |
T
|
F
|
T
|
P
|
L
|
I
|
Q
|
Y
|
G
|
C
|
90 |
S
|
S
|
Q
|
L
|
Q
|
F
|
F
|
L
|
C
|
S
|
100 |
V
|
Y
|
V
|
P
|
M
|
C
|
T
|
E
|
K
|
I
|
110 |
N
|
I
|
P
|
I
|
G
|
P
|
C
|
G
|
G
|
M
|
120 |
C
|
L
|
S
|
V
|
K
|
R
|
R
|
C
|
E
|
P
|
130 |
V
|
L
|
K
|
E
|
F
|
G
|
F
|
A
|
W
|
P
|
140 |
E
|
S
|
L
|
N
|
C
|
S
|
K
|
F
|
P
|
P
|
150 |
Q
|
N
|
D
|
H
|
N
|
H
|
M
|
C
|
M
|
E
|
160 |
G
|
P
|
G
|
D
|
E
|
E
|
V
|
P
|
L
|
P
|
170 |
H
|
K
|
T
|
P
|
I
|
Q
|
P
|
G
|
E
|
E
|
180 |
C
|
H
|
S
|
V
|
G
|
T
|
N
|
S
|
D
|
Q
|
190 |
Y
|
I
|
W
|
V
|
K
|
R
|
S
|
L
|
N
|
C
|
200 |
V
|
L
|
K
|
C
|
G
|
Y
|
D
|
A
|
G
|
L
|
210 |
TM1 | |||||||||
Y
|
S
|
R
|
S
|
A
|
K
|
E
|
F
|
T
|
D
|
220 |
I
|
W
|
M
|
A
|
V
|
W
|
A
|
S
|
L
|
C
|
230 |
F
|
I
|
S
|
T
|
A
|
F
|
T
|
V
|
L
|
T
|
240 |
ICL1 | |||||||||
F
|
L
|
I
|
D
|
S
|
S
|
R
|
F
|
S
|
Y
|
250 |
TM2 | |||||||||
P
|
E
|
R
|
P
|
I
|
I
|
F
|
L
|
S
|
M
|
260 |
C
|
Y
|
N
|
I
|
Y
|
S
|
I
|
A
|
Y
|
I
|
270 |
ECL1 | |||||||||
V
|
R
|
L
|
T
|
V
|
G
|
R
|
E
|
R
|
I
|
280 |
S
|
C
|
D
|
F
|
E
|
E
|
A
|
A
|
E
|
P
|
290 |
V
|
L
|
I
|
Q
|
E
|
G
|
L
|
K
|
N
|
T
|
300 |
TM3 | |||||||||
G
|
C
|
A
|
I
|
I
|
F
|
L
|
L
|
M
|
Y
|
310 |
F
|
F
|
G
|
M
|
A
|
S
|
S
|
I
|
W
|
W
|
320 |
V
|
I
|
L
|
T
|
L
|
T
|
W
|
F
|
L
|
A
|
330 |
ICL2 | TM4 | ||||||||
A
|
G
|
L
|
K
|
W
|
G
|
H
|
E
|
A
|
I
|
340 |
E
|
M
|
H
|
S
|
S
|
Y
|
F
|
H
|
I
|
A
|
350 |
A
|
W
|
A
|
I
|
P
|
A
|
V
|
K
|
T
|
I
|
360 |
ECL2 | |||||||||
V
|
I
|
L
|
I
|
M
|
R
|
L
|
V
|
D
|
A
|
370 |
D
|
E
|
L
|
T
|
G
|
L
|
C
|
Y
|
V
|
G
|
380 |
TM5 | |||||||||
N
|
Q
|
N
|
L
|
D
|
A
|
L
|
T
|
G
|
F
|
390 |
V
|
V
|
A
|
P
|
L
|
F
|
T
|
Y
|
L
|
V
|
400 |
I
|
G
|
T
|
L
|
F
|
I
|
A
|
A
|
G
|
L
|
410 |
V
|
A
|
L
|
F
|
K
|
I
|
R
|
S
|
N
|
L
|
420 |
ICL3 | |||||||||
Q
|
K
|
D
|
G
|
T
|
K
|
T
|
D
|
K
|
L
|
430 |
TM6 | |||||||||
E
|
R
|
L
|
M
|
V
|
K
|
I
|
G
|
V
|
F
|
440 |
S
|
V
|
L
|
Y
|
T
|
V
|
P
|
A
|
T
|
C
|
450 |
V
|
I
|
A
|
C
|
Y
|
F
|
Y
|
E
|
I
|
S
|
460 |
ECL3 | |||||||||
N
|
W
|
A
|
L
|
F
|
R
|
Y
|
S
|
A
|
D
|
470 |
TM7 | |||||||||
D
|
S
|
N
|
M
|
A
|
V
|
E
|
M
|
L
|
K
|
480 |
I
|
F
|
M
|
S
|
L
|
L
|
V
|
G
|
I
|
T
|
490 |
H8 | |||||||||
S
|
G
|
M
|
W
|
I
|
W
|
S
|
A
|
K
|
T
|
500 |
L
|
H
|
T
|
W
|
Q
|
K
|
C
|
S
|
N
|
R
|
510 |
C-term | |||||||||
L
|
V
|
N
|
S
|
G
|
K
|
V
|
K
|
R
|
E
|
520 |
K
|
R
|
G
|
N
|
G
|
W
|
V
|
K
|
P
|
G
|
530 |
K
|
G
|
S
|
E
|
T
|
V
|
V
|
N-term | |||||||||
M
|
A
|
W
|
R
|
G
|
A
|
G
|
P
|
S
|
V
|
10 |
P
|
G
|
A
|
P
|
G
|
G
|
V
|
G
|
L
|
S
|
20 |
L
|
G
|
L
|
L
|
L
|
Q
|
L
|
L
|
L
|
L
|
30 |
L
|
G
|
P
|
A
|
R
|
G
|
F
|
G
|
D
|
E
|
40 |
E
|
E
|
R
|
R
|
C
|
D
|
P
|
I
|
R
|
I
|
50 |
S
|
M
|
C
|
Q
|
N
|
L
|
G
|
Y
|
N
|
V
|
60 |
T
|
K
|
M
|
P
|
N
|
L
|
V
|
G
|
H
|
E
|
70 |
L
|
Q
|
T
|
D
|
A
|
E
|
L
|
Q
|
L
|
T
|
80 |
T
|
F
|
T
|
P
|
L
|
I
|
Q
|
Y
|
G
|
C
|
90 |
S
|
S
|
Q
|
L
|
Q
|
F
|
F
|
L
|
C
|
S
|
100 |
V
|
Y
|
V
|
P
|
M
|
C
|
T
|
E
|
K
|
I
|
110 |
N
|
I
|
P
|
I
|
G
|
P
|
C
|
G
|
G
|
M
|
120 |
C
|
L
|
S
|
V
|
K
|
R
|
R
|
C
|
E
|
P
|
130 |
V
|
L
|
K
|
E
|
F
|
G
|
F
|
A
|
W
|
P
|
140 |
E
|
S
|
L
|
N
|
C
|
S
|
K
|
F
|
P
|
P
|
150 |
Q
|
N
|
D
|
H
|
N
|
H
|
M
|
C
|
M
|
E
|
160 |
G
|
P
|
G
|
D
|
E
|
E
|
V
|
P
|
L
|
P
|
170 |
H
|
K
|
T
|
P
|
I
|
Q
|
P
|
G
|
E
|
E
|
180 |
C
|
H
|
S
|
V
|
G
|
T
|
N
|
S
|
D
|
Q
|
190 |
Y
|
I
|
W
|
V
|
K
|
R
|
S
|
L
|
N
|
C
|
200 |
V
|
L
|
K
|
C
|
G
|
Y
|
D
|
A
|
G
|
L
|
210 |
TM1 | |||||||||
Y
|
S
|
R
|
S
|
A
|
K
|
E
|
F
|
T
|
D
|
220 |
I
|
W
|
M
|
A
|
V
|
W
|
A
|
S
|
L
|
C
|
230 |
F
|
I
|
S
|
T
|
A
|
F
|
T
|
V
|
L
|
T
|
240 |
ICL1 | |||||||||
F
|
L
|
I
|
D
|
S
|
S
|
R
|
F
|
S
|
Y
|
250 |
TM2 | |||||||||
P
|
E
|
R
|
P
|
I
|
I
|
F
|
L
|
S
|
M
|
260 |
C
|
Y
|
N
|
I
|
Y
|
S
|
I
|
A
|
Y
|
I
|
270 |
ECL1 | |||||||||
V
|
R
|
L
|
T
|
V
|
G
|
R
|
E
|
R
|
I
|
280 |
S
|
C
|
D
|
F
|
E
|
E
|
A
|
A
|
E
|
P
|
290 |
V
|
L
|
I
|
Q
|
E
|
G
|
L
|
K
|
N
|
T
|
300 |
TM3 | |||||||||
G
|
C
|
A
|
I
|
I
|
F
|
L
|
L
|
M
|
Y
|
310 |
F
|
F
|
G
|
M
|
A
|
S
|
S
|
I
|
W
|
W
|
320 |
V
|
I
|
L
|
T
|
L
|
T
|
W
|
F
|
L
|
A
|
330 |
ICL2 | TM4 | ||||||||
A
|
G
|
L
|
K
|
W
|
G
|
H
|
E
|
A
|
I
|
340 |
E
|
M
|
H
|
S
|
S
|
Y
|
F
|
H
|
I
|
A
|
350 |
A
|
W
|
A
|
I
|
P
|
A
|
V
|
K
|
T
|
I
|
360 |
ECL2 | |||||||||
V
|
I
|
L
|
I
|
M
|
R
|
L
|
V
|
D
|
A
|
370 |
D
|
E
|
L
|
T
|
G
|
L
|
C
|
Y
|
V
|
G
|
380 |
TM5 | |||||||||
N
|
Q
|
N
|
L
|
D
|
A
|
L
|
T
|
G
|
F
|
390 |
V
|
V
|
A
|
P
|
L
|
F
|
T
|
Y
|
L
|
V
|
400 |
I
|
G
|
T
|
L
|
F
|
I
|
A
|
A
|
G
|
L
|
410 |
V
|
A
|
L
|
F
|
K
|
I
|
R
|
S
|
N
|
L
|
420 |
ICL3 | |||||||||
Q
|
K
|
D
|
G
|
T
|
K
|
T
|
D
|
K
|
L
|
430 |
TM6 | |||||||||
E
|
R
|
L
|
M
|
V
|
K
|
I
|
G
|
V
|
F
|
440 |
S
|
V
|
L
|
Y
|
T
|
V
|
P
|
A
|
T
|
C
|
450 |
V
|
I
|
A
|
C
|
Y
|
F
|
Y
|
E
|
I
|
S
|
460 |
ECL3 | |||||||||
N
|
W
|
A
|
L
|
F
|
R
|
Y
|
S
|
A
|
D
|
470 |
TM7 | |||||||||
D
|
S
|
N
|
M
|
A
|
V
|
E
|
M
|
L
|
K
|
480 |
I
|
F
|
M
|
S
|
L
|
L
|
V
|
G
|
I
|
T
|
490 |
H8 | |||||||||
S
|
G
|
M
|
W
|
I
|
W
|
S
|
A
|
K
|
T
|
500 |
L
|
H
|
T
|
W
|
Q
|
K
|
C
|
S
|
N
|
R
|
510 |
C-term | |||||||||
L
|
V
|
N
|
S
|
G
|
K
|
V
|
K
|
R
|
E
|
520 |
K
|
R
|
G
|
N
|
G
|
W
|
V
|
K
|
P
|
G
|
530 |
K
|
G
|
S
|
E
|
T
|
V
|
V
|
537 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|