mchr1_human
NAME
8WSS
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
mchr1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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S
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V
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G
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A
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M
|
K
|
K
|
G
|
V
|
10 |
|
|
|
|
|
|
|
|
|
|
G
|
R
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A
|
V
|
G
|
L
|
G
|
G
|
G
|
S
|
20 |
|
|
|
|
|
|
|
|
|
|
G
|
C
|
Q
|
A
|
T
|
E
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E
|
D
|
P
|
L
|
30 |
|
|
|
|
|
|
|
|
|
|
P
|
N
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C
|
G
|
A
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C
|
A
|
P
|
G
|
Q
|
40 |
|
|
|
|
|
|
|
|
|
|
G
|
G
|
R
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R
|
W
|
R
|
L
|
P
|
Q
|
P
|
50 |
|
|
|
|
|
|
|
|
|
|
A
|
W
|
V
|
E
|
G
|
S
|
S
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A
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R
|
L
|
60 |
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|
|
|
|
|
|
|
|
|
W
|
E
|
Q
|
A
|
T
|
G
|
T
|
G
|
W
|
M
|
70 |
D
|
L
|
E
|
A
|
S
|
L
|
L
|
P
|
T
|
G
|
80 |
P
|
N
|
A
|
S
|
N
|
T
|
S
|
D
|
G
|
P
|
90 |
D
|
N
|
L
|
T
|
S
|
A
|
G
|
S
|
P
|
P
|
100 |
TM1 | |||||||||
R
|
T
|
G
|
S
|
I
|
S
|
Y
|
I
|
N
|
I
|
110 |
I
|
M
|
P
|
S
|
V
|
F
|
G
|
T
|
I
|
C
|
120 |
L
|
L
|
G
|
I
|
I
|
G
|
N
|
S
|
T
|
V
|
130 |
I
|
F
|
A
|
V
|
V
|
K
|
K
|
S
|
K
|
L
|
140 |
ICL1 | TM2 | ||||||||
H
|
W
|
C
|
N
|
N
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V
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P
|
D
|
I
|
F
|
150 |
I
|
I
|
N
|
L
|
S
|
V
|
V
|
D
|
L
|
L
|
160 |
F
|
L
|
L
|
G
|
M
|
P
|
F
|
M
|
I
|
H
|
170 |
ECL1 | |||||||||
Q
|
L
|
M
|
G
|
N
|
G
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V
|
W
|
H
|
F
|
180 |
TM3 | |||||||||
G
|
E
|
T
|
M
|
C
|
T
|
L
|
I
|
T
|
A
|
190 |
M
|
D
|
A
|
N
|
S
|
Q
|
F
|
T
|
S
|
T
|
200 |
Y
|
I
|
L
|
T
|
A
|
M
|
A
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I
|
D
|
R
|
210 |
ICL2 | |||||||||
Y
|
L
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A
|
T
|
V
|
H
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P
|
I
|
S
|
S
|
220 |
TM4 | |||||||||
T
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K
|
F
|
R
|
K
|
P
|
S
|
V
|
A
|
T
|
230 |
L
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V
|
I
|
C
|
L
|
L
|
W
|
A
|
L
|
S
|
240 |
F
|
I
|
S
|
I
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T
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P
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V
|
W
|
L
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Y
|
250 |
ECL2 | |||||||||
A
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R
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L
|
I
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P
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F
|
P
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G
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G
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A
|
260 |
V
|
G
|
C
|
G
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I
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R
|
L
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P
|
N
|
P
|
270 |
TM5 | |||||||||
D
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T
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D
|
L
|
Y
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W
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F
|
T
|
L
|
Y
|
280 |
Q
|
F
|
F
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L
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A
|
F
|
A
|
L
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P
|
F
|
290 |
V
|
V
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I
|
T
|
A
|
A
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Y
|
V
|
R
|
I
|
300 |
ICL3 | |||||||||
L
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Q
|
R
|
M
|
T
|
S
|
S
|
V
|
A
|
P
|
310 |
TM6 | |||||||||
A
|
S
|
Q
|
R
|
S
|
I
|
R
|
L
|
R
|
T
|
320 |
K
|
R
|
V
|
T
|
R
|
T
|
A
|
I
|
A
|
I
|
330 |
C
|
L
|
V
|
F
|
F
|
V
|
C
|
W
|
A
|
P
|
340 |
Y
|
Y
|
V
|
L
|
Q
|
L
|
T
|
Q
|
L
|
S
|
350 |
ECL3 | TM7 | ||||||||
I
|
S
|
R
|
P
|
T
|
L
|
T
|
F
|
V
|
Y
|
360 |
L
|
Y
|
N
|
A
|
A
|
I
|
S
|
L
|
G
|
Y
|
370 |
A
|
N
|
S
|
C
|
L
|
N
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P
|
F
|
V
|
Y
|
380 |
H8 | |||||||||
I
|
V
|
L
|
C
|
E
|
T
|
F
|
R
|
K
|
R
|
390 |
L
|
V
|
L
|
S
|
V
|
K
|
P
|
A
|
A
|
Q
|
400 |
|
|
|
|
|
|||||
C-term | |||||||||
G
|
Q
|
L
|
R
|
A
|
V
|
S
|
N
|
A
|
Q
|
410 |
|
|
|
|
|
|
|
|
|
|
T
|
A
|
D
|
E
|
E
|
R
|
T
|
E
|
S
|
K
|
420 |
|
|
G
|
T
|
|
|||||||||
N-term | |||||||||
|
M
|
D
|
L
|
E
|
A
|
S
|
L
|
L
|
P
|
78 |
T
|
G
|
P
|
N
|
A
|
S
|
N
|
T
|
S
|
D
|
88 |
G
|
P
|
D
|
N
|
L
|
T
|
S
|
A
|
G
|
S
|
98 |
TM1 | |||||||||
P
|
P
|
R
|
T
|
G
|
S
|
I
|
S
|
Y
|
I
|
108 |
N
|
I
|
I
|
M
|
P
|
S
|
V
|
F
|
G
|
T
|
118 |
I
|
C
|
L
|
L
|
G
|
I
|
I
|
G
|
N
|
S
|
128 |
T
|
V
|
I
|
F
|
A
|
V
|
V
|
K
|
K
|
S
|
138 |
ICL1 | TM2 | ||||||||
K
|
L
|
H
|
W
|
C
|
N
|
N
|
V
|
P
|
D
|
148 |
I
|
F
|
I
|
I
|
N
|
L
|
S
|
V
|
V
|
D
|
158 |
L
|
L
|
F
|
L
|
L
|
G
|
M
|
P
|
F
|
M
|
168 |
ECL1 | |||||||||
I
|
H
|
Q
|
L
|
M
|
G
|
N
|
G
|
V
|
W
|
178 |
TM3 | |||||||||
H
|
F
|
G
|
E
|
T
|
M
|
C
|
T
|
L
|
I
|
188 |
T
|
A
|
M
|
D
|
A
|
N
|
S
|
Q
|
F
|
T
|
198 |
S
|
T
|
Y
|
I
|
L
|
T
|
A
|
M
|
A
|
I
|
208 |
D
|
R
|
Y
|
L
|
A
|
T
|
V
|
H
|
P
|
I
|
218 |
ICL2 | TM4 | ||||||||
S
|
S
|
T
|
K
|
F
|
R
|
K
|
P
|
S
|
V
|
228 |
A
|
T
|
L
|
V
|
I
|
C
|
L
|
L
|
W
|
A
|
238 |
L
|
S
|
F
|
I
|
S
|
I
|
T
|
P
|
V
|
W
|
248 |
ECL2 | |||||||||
L
|
Y
|
A
|
R
|
L
|
I
|
P
|
F
|
P
|
G
|
258 |
G
|
A
|
V
|
G
|
C
|
G
|
I
|
R
|
L
|
P
|
268 |
TM5 | |||||||||
N
|
P
|
D
|
T
|
D
|
L
|
Y
|
W
|
F
|
T
|
278 |
L
|
Y
|
Q
|
F
|
F
|
L
|
A
|
F
|
A
|
L
|
288 |
P
|
F
|
V
|
V
|
I
|
T
|
A
|
A
|
Y
|
V
|
298 |
ICL3 | |||||||||
R
|
I
|
L
|
Q
|
R
|
M
|
T
|
S
|
S
|
V
|
308 |
TM6 | |||||||||
A
|
P
|
A
|
S
|
Q
|
R
|
S
|
I
|
R
|
L
|
318 |
R
|
T
|
K
|
R
|
V
|
T
|
R
|
T
|
A
|
I
|
328 |
A
|
I
|
C
|
L
|
V
|
F
|
F
|
V
|
C
|
W
|
338 |
A
|
P
|
Y
|
Y
|
V
|
L
|
Q
|
L
|
T
|
Q
|
348 |
ECL3 | TM7 | ||||||||
L
|
S
|
I
|
S
|
R
|
P
|
T
|
L
|
T
|
F
|
358 |
V
|
Y
|
L
|
Y
|
N
|
A
|
A
|
I
|
S
|
L
|
368 |
G
|
Y
|
A
|
N
|
S
|
C
|
L
|
N
|
P
|
F
|
378 |
H8 | |||||||||
V
|
Y
|
I
|
V
|
L
|
C
|
E
|
T
|
F
|
R
|
388 |
K
|
R
|
L
|
V
|
L
|
S
|
V
|
K
|
P
|
A
|
398 |
|
|||||||
C-term | |||||||
A
|
Q
|
G
|
Q
|
L
|
R
|
A
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|