lgr4_human
NAME
8WVX
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
lgr4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
P
|
G
|
P
|
L
|
G
|
L
|
L
|
C
|
F
|
10 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
L
|
G
|
L
|
L
|
G
|
S
|
A
|
G
|
20 |
|
|
|
|||||||
P
|
S
|
G
|
A
|
A
|
P
|
P
|
L
|
C
|
A
|
30 |
A
|
P
|
C
|
S
|
C
|
D
|
G
|
D
|
R
|
R
|
40 |
V
|
D
|
C
|
S
|
G
|
K
|
G
|
L
|
T
|
A
|
50 |
V
|
P
|
E
|
G
|
L
|
S
|
A
|
F
|
T
|
Q
|
60 |
A
|
L
|
D
|
I
|
S
|
M
|
N
|
N
|
I
|
T
|
70 |
Q
|
L
|
P
|
E
|
D
|
A
|
F
|
K
|
N
|
F
|
80 |
P
|
F
|
L
|
E
|
E
|
L
|
Q
|
L
|
A
|
G
|
90 |
N
|
D
|
L
|
S
|
F
|
I
|
H
|
P
|
K
|
A
|
100 |
L
|
S
|
G
|
L
|
K
|
E
|
L
|
K
|
V
|
L
|
110 |
T
|
L
|
Q
|
N
|
N
|
Q
|
L
|
K
|
T
|
V
|
120 |
P
|
S
|
E
|
A
|
I
|
R
|
G
|
L
|
S
|
A
|
130 |
L
|
Q
|
S
|
L
|
R
|
L
|
D
|
A
|
N
|
H
|
140 |
I
|
T
|
S
|
V
|
P
|
E
|
D
|
S
|
F
|
E
|
150 |
G
|
L
|
V
|
Q
|
L
|
R
|
H
|
L
|
W
|
L
|
160 |
D
|
D
|
N
|
S
|
L
|
T
|
E
|
V
|
P
|
V
|
170 |
H
|
P
|
L
|
S
|
N
|
L
|
P
|
T
|
L
|
Q
|
180 |
A
|
L
|
T
|
L
|
A
|
L
|
N
|
K
|
I
|
S
|
190 |
S
|
I
|
P
|
D
|
F
|
A
|
F
|
T
|
N
|
L
|
200 |
S
|
S
|
L
|
V
|
V
|
L
|
H
|
L
|
H
|
N
|
210 |
N
|
K
|
I
|
R
|
S
|
L
|
S
|
Q
|
H
|
C
|
220 |
F
|
D
|
G
|
L
|
D
|
N
|
L
|
E
|
T
|
L
|
230 |
D
|
L
|
N
|
Y
|
N
|
N
|
L
|
G
|
E
|
F
|
240 |
P
|
Q
|
A
|
I
|
K
|
A
|
L
|
P
|
S
|
L
|
250 |
K
|
E
|
L
|
G
|
F
|
H
|
S
|
N
|
S
|
I
|
260 |
S
|
V
|
I
|
P
|
D
|
G
|
A
|
F
|
D
|
G
|
270 |
N
|
P
|
L
|
L
|
R
|
T
|
I
|
H
|
L
|
Y
|
280 |
D
|
N
|
P
|
L
|
S
|
F
|
V
|
G
|
N
|
S
|
290 |
A
|
F
|
H
|
N
|
L
|
S
|
D
|
L
|
H
|
S
|
300 |
L
|
V
|
I
|
R
|
G
|
A
|
S
|
M
|
V
|
Q
|
310 |
Q
|
F
|
P
|
N
|
L
|
T
|
G
|
T
|
V
|
H
|
320 |
L
|
E
|
S
|
L
|
T
|
L
|
T
|
G
|
T
|
K
|
330 |
I
|
S
|
S
|
I
|
P
|
N
|
N
|
L
|
C
|
Q
|
340 |
E
|
Q
|
K
|
M
|
L
|
R
|
T
|
L
|
D
|
L
|
350 |
S
|
Y
|
N
|
N
|
I
|
R
|
D
|
L
|
P
|
S
|
360 |
F
|
N
|
G
|
C
|
H
|
A
|
L
|
E
|
E
|
I
|
370 |
S
|
L
|
Q
|
R
|
N
|
Q
|
I
|
Y
|
Q
|
I
|
380 |
K
|
E
|
G
|
T
|
F
|
Q
|
G
|
L
|
I
|
S
|
390 |
L
|
R
|
I
|
L
|
D
|
L
|
S
|
R
|
N
|
L
|
400 |
I
|
H
|
E
|
I
|
H
|
S
|
R
|
A
|
F
|
A
|
410 |
T
|
L
|
G
|
P
|
I
|
T
|
N
|
L
|
D
|
V
|
420 |
S
|
F
|
N
|
E
|
L
|
T
|
S
|
F
|
P
|
T
|
430 |
E
|
G
|
L
|
N
|
G
|
L
|
N
|
Q
|
L
|
K
|
440 |
L
|
V
|
G
|
N
|
F
|
K
|
L
|
K
|
E
|
A
|
450 |
L
|
A
|
A
|
K
|
D
|
F
|
V
|
N
|
L
|
R
|
460 |
S
|
L
|
S
|
V
|
P
|
Y
|
A
|
Y
|
Q
|
C
|
470 |
C
|
A
|
F
|
W
|
G
|
C
|
D
|
S
|
Y
|
A
|
480 |
N
|
L
|
N
|
T
|
E
|
D
|
N
|
S
|
L
|
Q
|
490 |
D
|
H
|
S
|
V
|
A
|
Q
|
E
|
K
|
G
|
T
|
500 |
A
|
D
|
A
|
A
|
N
|
V
|
T
|
S
|
T
|
L
|
510 |
E
|
N
|
E
|
E
|
H
|
S
|
Q
|
I
|
I
|
I
|
520 |
H
|
C
|
T
|
P
|
S
|
T
|
G
|
A
|
F
|
K
|
530 |
TM1 | |||||||||
P
|
C
|
E
|
Y
|
L
|
L
|
G
|
S
|
W
|
M
|
540 |
I
|
R
|
L
|
T
|
V
|
W
|
F
|
I
|
F
|
L
|
550 |
V
|
A
|
L
|
F
|
F
|
N
|
L
|
L
|
V
|
I
|
560 |
ICL1 | |||||||||
L
|
T
|
T
|
F
|
A
|
S
|
C
|
T
|
S
|
L
|
570 |
TM2 | |||||||||
P
|
S
|
S
|
K
|
L
|
F
|
I
|
G
|
L
|
I
|
580 |
S
|
V
|
S
|
N
|
L
|
F
|
M
|
G
|
I
|
Y
|
590 |
T
|
G
|
I
|
L
|
T
|
F
|
L
|
D
|
A
|
V
|
600 |
ECL1 | |||||||||
S
|
W
|
G
|
R
|
F
|
A
|
E
|
F
|
G
|
I
|
610 |
TM3 | |||||||||
W
|
W
|
E
|
T
|
G
|
S
|
G
|
C
|
K
|
V
|
620 |
A
|
G
|
F
|
L
|
A
|
V
|
F
|
S
|
S
|
E
|
630 |
S
|
A
|
I
|
F
|
L
|
L
|
M
|
L
|
A
|
T
|
640 |
V
|
E
|
R
|
S
|
L
|
S
|
A
|
K
|
D
|
I
|
650 |
ICL2 | TM4 | ||||||||
M
|
K
|
N
|
G
|
K
|
S
|
N
|
H
|
L
|
K
|
660 |
Q
|
F
|
R
|
V
|
A
|
A
|
L
|
L
|
A
|
F
|
670 |
L
|
G
|
A
|
T
|
V
|
A
|
G
|
C
|
F
|
P
|
680 |
ECL2 | |||||||||
L
|
F
|
H
|
R
|
G
|
E
|
Y
|
S
|
A
|
S
|
690 |
P
|
L
|
C
|
L
|
P
|
F
|
P
|
T
|
G
|
E
|
700 |
TM5 | |||||||||
T
|
P
|
S
|
L
|
G
|
F
|
T
|
V
|
T
|
L
|
710 |
V
|
L
|
L
|
N
|
S
|
L
|
A
|
F
|
L
|
L
|
720 |
M
|
A
|
V
|
I
|
Y
|
T
|
K
|
L
|
Y
|
C
|
730 |
ICL3 | |||||||||
N
|
L
|
E
|
K
|
E
|
D
|
L
|
S
|
E
|
N
|
740 |
TM6 | |||||||||
S
|
Q
|
S
|
S
|
M
|
I
|
K
|
H
|
V
|
A
|
750 |
W
|
L
|
I
|
F
|
T
|
N
|
C
|
I
|
F
|
F
|
760 |
C
|
P
|
V
|
A
|
F
|
F
|
S
|
F
|
A
|
P
|
770 |
ECL3 | TM7 | ||||||||
L
|
I
|
T
|
A
|
I
|
S
|
I
|
S
|
P
|
E
|
780 |
I
|
M
|
K
|
S
|
V
|
T
|
L
|
I
|
F
|
F
|
790 |
P
|
L
|
P
|
A
|
C
|
L
|
N
|
P
|
V
|
L
|
800 |
H8 | |||||||||
Y
|
V
|
F
|
F
|
N
|
P
|
K
|
F
|
K
|
E
|
810 |
D
|
W
|
K
|
L
|
L
|
K
|
R
|
R
|
V
|
T
|
820 |
C-term | |||||||||
K
|
K
|
S
|
G
|
S
|
V
|
S
|
V
|
S
|
I
|
830 |
|
|
|
|
|
|
|
|
||
S
|
S
|
Q
|
G
|
G
|
C
|
L
|
E
|
Q
|
D
|
840 |
|
|
|
|
|
|
|
|
|
|
F
|
Y
|
Y
|
D
|
C
|
G
|
M
|
Y
|
S
|
H
|
850 |
|
|
|
|
|
|
|
|
|
|
L
|
Q
|
G
|
N
|
L
|
T
|
V
|
C
|
D
|
C
|
860 |
|
|
|
|
|
|
|
|
|
|
C
|
E
|
S
|
F
|
L
|
L
|
T
|
K
|
P
|
V
|
870 |
|
|
|
|
|
|
|
|
|
|
S
|
C
|
K
|
H
|
L
|
I
|
K
|
S
|
H
|
S
|
880 |
|
|
|
|
|
|
|
|
|
|
C
|
P
|
A
|
L
|
A
|
V
|
A
|
S
|
C
|
Q
|
890 |
|
|
|
|
|
|
|
|
|
|
R
|
P
|
E
|
G
|
Y
|
W
|
S
|
D
|
C
|
G
|
900 |
|
|
|
|
|
|
|
|
|
|
T
|
Q
|
S
|
A
|
H
|
S
|
D
|
Y
|
A
|
D
|
910 |
|
|
|
|
|
|
|
|
|
|
E
|
E
|
D
|
S
|
F
|
V
|
S
|
D
|
S
|
S
|
920 |
|
|
|
|
|
|
|
|
|
|
D
|
Q
|
V
|
Q
|
A
|
C
|
G
|
R
|
A
|
C
|
930 |
|
|
|
|
|
|
|
|
|
|
F
|
Y
|
Q
|
S
|
R
|
G
|
F
|
P
|
L
|
V
|
940 |
|
|
|
|
|
|
|
|
|
|
R
|
Y
|
A
|
Y
|
N
|
L
|
P
|
R
|
V
|
K
|
950 |
|
D
|
|
|||||||||
N-term | |||||||||
|
A
|
A
|
P
|
P
|
L
|
C
|
A
|
A
|
P
|
32 |
C
|
S
|
C
|
D
|
G
|
D
|
R
|
R
|
V
|
D
|
42 |
C
|
S
|
G
|
K
|
G
|
L
|
T
|
A
|
V
|
P
|
52 |
E
|
G
|
L
|
S
|
A
|
F
|
T
|
Q
|
A
|
L
|
62 |
D
|
I
|
S
|
M
|
N
|
N
|
I
|
T
|
Q
|
L
|
72 |
P
|
E
|
D
|
A
|
F
|
K
|
N
|
F
|
P
|
F
|
82 |
L
|
E
|
E
|
L
|
Q
|
L
|
A
|
G
|
N
|
D
|
92 |
L
|
S
|
F
|
I
|
H
|
P
|
K
|
A
|
L
|
S
|
102 |
G
|
L
|
K
|
E
|
L
|
K
|
V
|
L
|
T
|
L
|
112 |
Q
|
N
|
N
|
Q
|
L
|
K
|
T
|
V
|
P
|
S
|
122 |
E
|
A
|
I
|
R
|
G
|
L
|
S
|
A
|
L
|
Q
|
132 |
S
|
L
|
R
|
L
|
D
|
A
|
N
|
H
|
I
|
T
|
142 |
S
|
V
|
P
|
E
|
D
|
S
|
F
|
E
|
G
|
L
|
152 |
V
|
Q
|
L
|
R
|
H
|
L
|
W
|
L
|
D
|
D
|
162 |
N
|
S
|
L
|
T
|
E
|
V
|
P
|
V
|
H
|
P
|
172 |
L
|
S
|
N
|
L
|
P
|
T
|
L
|
Q
|
A
|
L
|
182 |
T
|
L
|
A
|
L
|
N
|
K
|
I
|
S
|
S
|
I
|
192 |
P
|
D
|
F
|
A
|
F
|
T
|
N
|
L
|
S
|
S
|
202 |
L
|
V
|
V
|
L
|
H
|
L
|
H
|
N
|
N
|
K
|
212 |
I
|
R
|
S
|
L
|
S
|
Q
|
H
|
C
|
F
|
D
|
222 |
G
|
L
|
D
|
N
|
L
|
E
|
T
|
L
|
D
|
L
|
232 |
N
|
Y
|
N
|
N
|
L
|
G
|
E
|
F
|
P
|
Q
|
242 |
A
|
I
|
K
|
A
|
L
|
P
|
S
|
L
|
K
|
E
|
252 |
L
|
G
|
F
|
H
|
S
|
N
|
S
|
I
|
S
|
V
|
262 |
I
|
P
|
D
|
G
|
A
|
F
|
D
|
G
|
N
|
P
|
272 |
L
|
L
|
R
|
T
|
I
|
H
|
L
|
Y
|
D
|
N
|
282 |
P
|
L
|
S
|
F
|
V
|
G
|
N
|
S
|
A
|
F
|
292 |
H
|
N
|
L
|
S
|
D
|
L
|
H
|
S
|
L
|
V
|
302 |
I
|
R
|
G
|
A
|
S
|
M
|
V
|
Q
|
Q
|
F
|
312 |
P
|
N
|
L
|
T
|
G
|
T
|
V
|
H
|
L
|
E
|
322 |
S
|
L
|
T
|
L
|
T
|
G
|
T
|
K
|
I
|
S
|
332 |
S
|
I
|
P
|
N
|
N
|
L
|
C
|
Q
|
E
|
Q
|
342 |
K
|
M
|
L
|
R
|
T
|
L
|
D
|
L
|
S
|
Y
|
352 |
N
|
N
|
I
|
R
|
D
|
L
|
P
|
S
|
F
|
N
|
362 |
G
|
C
|
H
|
A
|
L
|
E
|
E
|
I
|
S
|
L
|
372 |
Q
|
R
|
N
|
Q
|
I
|
Y
|
Q
|
I
|
K
|
E
|
382 |
G
|
T
|
F
|
Q
|
G
|
L
|
I
|
S
|
L
|
R
|
392 |
I
|
L
|
D
|
L
|
S
|
R
|
N
|
L
|
I
|
H
|
402 |
E
|
I
|
H
|
S
|
R
|
A
|
F
|
A
|
T
|
L
|
412 |
G
|
P
|
I
|
T
|
N
|
L
|
D
|
V
|
S
|
F
|
422 |
N
|
E
|
L
|
T
|
S
|
F
|
P
|
T
|
E
|
G
|
432 |
L
|
N
|
G
|
L
|
N
|
Q
|
L
|
K
|
L
|
V
|
442 |
G
|
N
|
F
|
K
|
L
|
K
|
E
|
A
|
L
|
A
|
452 |
A
|
K
|
D
|
F
|
V
|
N
|
L
|
R
|
S
|
L
|
462 |
S
|
V
|
P
|
Y
|
A
|
Y
|
Q
|
C
|
C
|
A
|
472 |
F
|
W
|
G
|
C
|
D
|
S
|
Y
|
A
|
N
|
L
|
482 |
N
|
T
|
E
|
D
|
N
|
S
|
L
|
Q
|
D
|
H
|
492 |
S
|
V
|
A
|
Q
|
E
|
K
|
G
|
T
|
A
|
D
|
502 |
A
|
A
|
N
|
V
|
T
|
S
|
T
|
L
|
E
|
N
|
512 |
E
|
E
|
H
|
S
|
Q
|
I
|
I
|
I
|
H
|
C
|
522 |
T
|
P
|
S
|
T
|
G
|
A
|
F
|
K
|
P
|
C
|
532 |
TM1 | |||||||||
E
|
Y
|
L
|
L
|
G
|
S
|
W
|
M
|
I
|
R
|
542 |
L
|
T
|
V
|
W
|
F
|
I
|
F
|
L
|
V
|
A
|
552 |
L
|
F
|
F
|
N
|
L
|
L
|
V
|
I
|
L
|
T
|
562 |
ICL1 | |||||||||
T
|
F
|
A
|
S
|
C
|
T
|
S
|
L
|
P
|
S
|
572 |
TM2 | |||||||||
S
|
K
|
L
|
F
|
I
|
G
|
L
|
I
|
S
|
V
|
582 |
S
|
N
|
L
|
F
|
M
|
G
|
I
|
Y
|
T
|
G
|
592 |
I
|
L
|
T
|
F
|
L
|
D
|
A
|
V
|
S
|
W
|
602 |
ECL1 | |||||||||
G
|
R
|
F
|
A
|
E
|
F
|
G
|
I
|
W
|
W
|
612 |
TM3 | |||||||||
E
|
T
|
G
|
S
|
G
|
C
|
K
|
V
|
A
|
G
|
622 |
F
|
L
|
A
|
V
|
F
|
S
|
S
|
E
|
S
|
A
|
632 |
I
|
F
|
L
|
L
|
M
|
L
|
A
|
T
|
V
|
E
|
642 |
R
|
S
|
L
|
S
|
A
|
K
|
D
|
I
|
M
|
K
|
652 |
ICL2 | TM4 | ||||||||
N
|
G
|
K
|
S
|
N
|
H
|
L
|
K
|
Q
|
F
|
662 |
R
|
V
|
A
|
A
|
L
|
L
|
A
|
F
|
L
|
G
|
672 |
A
|
T
|
V
|
A
|
G
|
C
|
F
|
P
|
L
|
F
|
682 |
ECL2 | |||||||||
H
|
R
|
G
|
E
|
Y
|
S
|
A
|
S
|
P
|
L
|
692 |
C
|
L
|
P
|
F
|
P
|
T
|
G
|
E
|
T
|
P
|
702 |
TM5 | |||||||||
S
|
L
|
G
|
F
|
T
|
V
|
T
|
L
|
V
|
L
|
712 |
L
|
N
|
S
|
L
|
A
|
F
|
L
|
L
|
M
|
A
|
722 |
V
|
I
|
Y
|
T
|
K
|
L
|
Y
|
C
|
N
|
L
|
732 |
ICL3 | TM6 | ||||||||
E
|
K
|
E
|
D
|
L
|
S
|
E
|
N
|
S
|
Q
|
742 |
S
|
S
|
M
|
I
|
K
|
H
|
V
|
A
|
W
|
L
|
752 |
I
|
F
|
T
|
N
|
C
|
I
|
F
|
F
|
C
|
P
|
762 |
V
|
A
|
F
|
F
|
S
|
F
|
A
|
P
|
L
|
I
|
772 |
ECL3 | TM7 | ||||||||
T
|
A
|
I
|
S
|
I
|
S
|
P
|
E
|
I
|
M
|
782 |
K
|
S
|
V
|
T
|
L
|
I
|
F
|
F
|
P
|
L
|
792 |
P
|
A
|
C
|
L
|
N
|
P
|
V
|
L
|
Y
|
V
|
802 |
H8 | |||||||||
F
|
F
|
N
|
P
|
K
|
F
|
K
|
E
|
D
|
W
|
812 |
K
|
L
|
L
|
K
|
R
|
R
|
V
|
T
|
K
|
K
|
822 |
|
|||||||||||||||||||||
C-term | |||||||||||||||||||||
S
|
G
|
S
|
V
|
S
|
V
|
S
|
I
|
S
|
S
|
|
|||||||||||
832 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|