glr_human
NAME
8YW5
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
glr_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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P
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P
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C
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Q
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P
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Q
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R
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P
|
L
|
10 |
|
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|
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|
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|
|
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L
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L
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L
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L
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L
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L
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L
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A
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C
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Q
|
20 |
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||||
P
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Q
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V
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P
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S
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A
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Q
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V
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M
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D
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30 |
F
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L
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F
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E
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K
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W
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K
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L
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Y
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G
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40 |
D
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Q
|
C
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H
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H
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N
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L
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S
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L
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L
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50 |
P
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P
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P
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T
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E
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L
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V
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C
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N
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R
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60 |
T
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F
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D
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K
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Y
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S
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C
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W
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P
|
D
|
70 |
T
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P
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A
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N
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T
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T
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A
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N
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I
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S
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80 |
C
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P
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W
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Y
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L
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P
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W
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H
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H
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K
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90 |
V
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Q
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H
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R
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F
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V
|
F
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K
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R
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C
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100 |
G
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P
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D
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G
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Q
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W
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V
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R
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G
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P
|
110 |
R
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G
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Q
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P
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W
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R
|
D
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A
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S
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Q
|
120 |
C
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Q
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M
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D
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G
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E
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E
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I
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E
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V
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130 |
TM1 | |||||||||
Q
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K
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E
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V
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A
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K
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M
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Y
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S
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S
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140 |
F
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Q
|
V
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M
|
Y
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T
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V
|
G
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Y
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S
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150 |
L
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S
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L
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G
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A
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L
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L
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L
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A
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L
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160 |
ICL1 | |||||||||
A
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I
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L
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G
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G
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L
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S
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K
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L
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H
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170 |
TM2 | |||||||||
C
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T
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R
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N
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A
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I
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H
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A
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N
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L
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180 |
F
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A
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S
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F
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V
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L
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K
|
A
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S
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S
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190 |
V
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L
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V
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I
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D
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G
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L
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L
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R
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T
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200 |
ECL1 | |||||||||
R
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Y
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S
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Q
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K
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I
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G
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D
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D
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L
|
210 |
S
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V
|
S
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T
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W
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L
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S
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D
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G
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A
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220 |
TM3 | |||||||||
V
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A
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G
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C
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R
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V
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A
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A
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V
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F
|
230 |
M
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Q
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Y
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G
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I
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V
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A
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N
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Y
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C
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240 |
W
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L
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L
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V
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E
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G
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L
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Y
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L
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H
|
250 |
ICL2 | |||||||||
N
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L
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L
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G
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L
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A
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T
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L
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P
|
E
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260 |
TM4 | |||||||||
R
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S
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F
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F
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S
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L
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Y
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L
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G
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I
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270 |
G
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W
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G
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A
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P
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M
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L
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F
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V
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V
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280 |
P
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W
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A
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V
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V
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K
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C
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L
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F
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E
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290 |
ECL2 | |||||||||
N
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V
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Q
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C
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W
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T
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S
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N
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D
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N
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300 |
TM5 | |||||||||
M
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G
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F
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W
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W
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I
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L
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R
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F
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P
|
310 |
V
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F
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L
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A
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I
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L
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I
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N
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F
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F
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320 |
I
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F
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V
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R
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I
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V
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Q
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L
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L
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V
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330 |
ICL3 | |||||||||
A
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K
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L
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R
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A
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R
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Q
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M
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H
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H
|
340 |
TM6 | |||||||||
T
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D
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Y
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K
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F
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R
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L
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A
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K
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S
|
350 |
T
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L
|
T
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L
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I
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P
|
L
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L
|
G
|
V
|
360 |
H
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E
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V
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V
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F
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A
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F
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V
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T
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D
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370 |
ECL3 | TM7 | ||||||||
E
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H
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A
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Q
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G
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T
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L
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R
|
S
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A
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380 |
K
|
L
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F
|
F
|
D
|
L
|
F
|
L
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S
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S
|
390 |
F
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Q
|
G
|
L
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L
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V
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A
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V
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L
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Y
|
400 |
H8 | |||||||||
C
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F
|
L
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N
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K
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E
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V
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Q
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S
|
E
|
410 |
L
|
R
|
R
|
R
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W
|
H
|
R
|
W
|
R
|
L
|
420 |
G
|
K
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V
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L
|
W
|
E
|
E
|
R
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N
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T
|
430 |
|
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||
C-term | |||||||||
S
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N
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H
|
R
|
A
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S
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S
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S
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P
|
G
|
440 |
|
|
|
|
|
|
|
|
|
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H
|
G
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P
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P
|
S
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K
|
E
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L
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Q
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F
|
450 |
|
|
|
|
|
|
|
|
|
|
G
|
R
|
G
|
G
|
G
|
S
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Q
|
D
|
S
|
S
|
460 |
|
|
|
|
|
|
|
|
|
|
A
|
E
|
T
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P
|
L
|
A
|
G
|
G
|
L
|
P
|
470 |
|
|
|
|
|
|
|
R
|
L
|
A
|
E
|
S
|
P
|
F
|
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|||||||||
N-term | |||||||||
|
Q
|
V
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M
|
D
|
F
|
L
|
F
|
E
|
K
|
35 |
W
|
K
|
L
|
Y
|
G
|
D
|
Q
|
C
|
H
|
H
|
45 |
N
|
L
|
S
|
L
|
L
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P
|
P
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P
|
T
|
E
|
55 |
L
|
V
|
C
|
N
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R
|
T
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F
|
D
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K
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Y
|
65 |
S
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C
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W
|
P
|
D
|
T
|
P
|
A
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N
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T
|
75 |
T
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A
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N
|
I
|
S
|
C
|
P
|
W
|
Y
|
L
|
85 |
P
|
W
|
H
|
H
|
K
|
V
|
Q
|
H
|
R
|
F
|
95 |
V
|
F
|
K
|
R
|
C
|
G
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P
|
D
|
G
|
Q
|
105 |
W
|
V
|
R
|
G
|
P
|
R
|
G
|
Q
|
P
|
W
|
115 |
R
|
D
|
A
|
S
|
Q
|
C
|
Q
|
M
|
D
|
G
|
125 |
TM1 | |||||||||
E
|
E
|
I
|
E
|
V
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Q
|
K
|
E
|
V
|
A
|
135 |
K
|
M
|
Y
|
S
|
S
|
F
|
Q
|
V
|
M
|
Y
|
145 |
T
|
V
|
G
|
Y
|
S
|
L
|
S
|
L
|
G
|
A
|
155 |
L
|
L
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L
|
A
|
L
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A
|
I
|
L
|
G
|
G
|
165 |
ICL1 | TM2 | ||||||||
L
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S
|
K
|
L
|
H
|
C
|
T
|
R
|
N
|
A
|
175 |
I
|
H
|
A
|
N
|
L
|
F
|
A
|
S
|
F
|
V
|
185 |
L
|
K
|
A
|
S
|
S
|
V
|
L
|
V
|
I
|
D
|
195 |
G
|
L
|
L
|
R
|
T
|
R
|
Y
|
S
|
Q
|
K
|
205 |
ECL1 | |||||||||
I
|
G
|
D
|
D
|
L
|
S
|
V
|
S
|
T
|
W
|
215 |
TM3 | |||||||||
L
|
S
|
D
|
G
|
A
|
V
|
A
|
G
|
C
|
R
|
225 |
V
|
A
|
A
|
V
|
F
|
M
|
Q
|
Y
|
G
|
I
|
235 |
V
|
A
|
N
|
Y
|
C
|
W
|
L
|
L
|
V
|
E
|
245 |
G
|
L
|
Y
|
L
|
H
|
N
|
L
|
L
|
G
|
L
|
255 |
ICL2 | TM4 | ||||||||
A
|
T
|
L
|
P
|
E
|
R
|
S
|
F
|
F
|
S
|
265 |
L
|
Y
|
L
|
G
|
I
|
G
|
W
|
G
|
A
|
P
|
275 |
M
|
L
|
F
|
V
|
V
|
P
|
W
|
A
|
V
|
V
|
285 |
ECL2 | |||||||||
K
|
C
|
L
|
F
|
E
|
N
|
V
|
Q
|
C
|
W
|
295 |
T
|
S
|
N
|
D
|
N
|
M
|
G
|
F
|
W
|
W
|
305 |
TM5 | |||||||||
I
|
L
|
R
|
F
|
P
|
V
|
F
|
L
|
A
|
I
|
315 |
L
|
I
|
N
|
F
|
F
|
I
|
F
|
V
|
R
|
I
|
325 |
V
|
Q
|
L
|
L
|
V
|
A
|
K
|
L
|
R
|
A
|
335 |
ICL3 | TM6 | ||||||||
R
|
Q
|
M
|
H
|
H
|
T
|
D
|
Y
|
K
|
F
|
345 |
R
|
L
|
A
|
K
|
S
|
T
|
L
|
T
|
L
|
I
|
355 |
P
|
L
|
L
|
G
|
V
|
H
|
E
|
V
|
V
|
F
|
365 |
ECL3 | |||||||||
A
|
F
|
V
|
T
|
D
|
E
|
H
|
A
|
Q
|
G
|
375 |
TM7 | |||||||||
T
|
L
|
R
|
S
|
A
|
K
|
L
|
F
|
F
|
D
|
385 |
L
|
F
|
L
|
S
|
S
|
F
|
Q
|
G
|
L
|
L
|
395 |
H8 | |||||||||
V
|
A
|
V
|
L
|
Y
|
C
|
F
|
L
|
N
|
K
|
405 |
E
|
V
|
Q
|
S
|
E
|
L
|
R
|
R
|
R
|
W
|
415 |
H
|
R
|
W
|
R
|
L
|
G
|
K
|
V
|
L
|
W
|
425 |
|
|||||||||
C-term | |||||||||
E
|
E
|
R
|
N
|
T
|
S
|
N
|
|
||
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|