NTS1 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

20ZG

RESOLUTION

2.4 Å

REFERENCE

10.1038/S41586-026-10228-W

PUBLICATION DATE

June 3, 2026

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

58 interactions (Click to see)

LIGANDS

JMV449 - Agonist pubchem gtoplig

PHYSIOLOGICAL LIGANDS

large neuromedin N (Mouse), neurotensin (Human), neuromedin N (Human), large neuromedin N (Human), large neuromedin N (Rat), large neurotensin (Human), neuromedin N (Mouse)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Van-der-waals TM7 V 367 7x56 G.H5 F 354 G.H5.26
Hydrophobic TM6 H 300 6x32 G.H5 F 354 G.H5.26
Hydrophobic TM3 I 170 3x54 G.H5 L 353 G.H5.25
Hydrophobic ICL2 F 174 34x51 G.H5 I 343 G.H5.15
Van-der-waals ICL2 F 174 34x51 G.H5 I 343 G.H5.15
Hydrophobic TM5 L 263 5x65 G.H5 L 353 G.H5.25
Van-der-waals TM5 L 263 5x65 G.H5 L 353 G.H5.25
Van-der-waals TM7 V 367 7x56 G.H5 G 352 G.H5.24
Hydrophobic ICL2 F 174 34x51 G.H5 T 340 G.H5.12
Van-der-waals ICL2 F 174 34x51 G.H5 T 340 G.H5.12
Polar ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Polar ICL4 S 368 - G.H5 F 354 G.H5.26
Hydrophobic ICL4 S 368 - G.H5 F 354 G.H5.26
Van-der-waals ICL4 S 368 - G.H5 F 354 G.H5.26
Hydrophobic TM5 M 266 5x68 G.H5 I 344 G.H5.16
Hydrophobic ICL2 P 173 34x50 G.H5 I 344 G.H5.16
Hydrophobic TM5 L 263 5x65 G.H5 L 348 G.H5.20
Polar TM3 R 166 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 166 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM6 G 301 6x33 G.H5 L 353 G.H5.25
Polar TM2 Q 98 2x36 G.H5 D 350 G.H5.22
Hydrophobic TM2 Q 98 2x36 G.H5 D 350 G.H5.22
Van-der-waals TM2 Q 98 2x36 G.H5 D 350 G.H5.22
Hydrophobic TM2 L 97 2x35 G.H5 D 350 G.H5.22
Polar ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Hydrophobic TM3 I 170 3x54 G.H5 I 344 G.H5.16
Hydrophobic TM3 A 169 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 P 173 34x50 G.H5 I 343 G.H5.15
Hydrophobic ICL2 P 173 34x50 G.H5 T 340 G.H5.12
Hydrophobic TM4 S 181 4x38 G.HN E 28 G.HN.52
Hydrophobic TM6 L 305 6x37 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 305 6x37 G.H5 L 353 G.H5.25
Hydrophobic TM3 I 170 3x54 G.H5 C 351 G.H5.23
Hydrophobic TM6 V 304 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM2 V 101 2x39 G.H5 C 351 G.H5.23
Hydrophobic ICL2 F 174 34x51 G.s2s3 K 192 G.s2s3.01
Hydrophobic TM6 L 298 6x30 G.H5 L 348 G.H5.20
Van-der-waals TM6 L 298 6x30 G.H5 L 348 G.H5.20
Hydrophobic ICL2 K 177 34x54 G.S3 L 194 G.S3.01
Hydrophobic TM5 K 262 5x64 G.H5 I 344 G.H5.16
Hydrophobic ICL2 K 177 34x54 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 F 174 34x51 G.S3 L 194 G.S3.01
Van-der-waals ICL2 F 174 34x51 G.S3 L 194 G.S3.01