5-HT4 receptor - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

7XT8

RESOLUTION

3.1 Å

REFERENCE

10.1016/J.MOLCEL.2022.05.031

PUBLICATION DATE

July 27, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

46 interactions (Click to see)

LIGANDS

5-hydroxytryptamine - Agonist pubchem gtoplig chembl_ligand drugbank

PHYSIOLOGICAL LIGANDS

5-hydroxytryptamine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM5 I 225 5x72 G.h4s6 Y 358 G.h4s6.20
Hydrophobic TM5 G 232 5x79 G.H4 R 347 G.H4.17
Van-der-waals TM5 G 232 5x79 G.H4 R 347 G.H4.17
Polar TM6 K 255 6x32 G.H5 L 394 G.H5.26
Hydrophobic TM6 K 255 6x32 G.H5 L 394 G.H5.26
Van-der-waals TM6 K 255 6x32 G.H5 L 394 G.H5.26
Hydrophobic TM3 I 121 3x54 G.H5 Y 391 G.H5.23
Van-der-waals TM3 I 121 3x54 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 P 125 34x50 G.H5 I 383 G.H5.15
Van-der-waals ICL2 P 125 34x50 G.H5 I 383 G.H5.15
Ionic TM6 K 255 6x32 G.H5 E 392 G.H5.24
Polar TM6 K 255 6x32 G.H5 E 392 G.H5.24
Hydrophobic TM6 K 255 6x32 G.H5 E 392 G.H5.24
Van-der-waals TM6 K 255 6x32 G.H5 E 392 G.H5.24
Hydrophobic ICL2 V 127 34x52 G.S3 V 217 G.S3.01
Hydrophobic TM5 Q 229 5x76 G.h4s6 T 350 G.h4s6.03
Van-der-waals TM5 Q 229 5x76 G.h4s6 T 350 G.h4s6.03
Van-der-waals TM7 L 314 7x56 G.H5 Y 391 G.H5.23
Polar TM3 I 121 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 I 121 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 I 121 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM5 L 228 5x75 G.S6 C 359 G.S6.01
Hydrophobic TM3 I 121 3x54 G.H5 L 393 G.H5.25
Hydrophobic ICL2 L 126 34x51 G.S3 F 219 G.S3.03
Van-der-waals ICL2 L 126 34x51 G.S3 F 219 G.S3.03
Polar ICL2 N 130 34x55 G.hns1 A 39 G.hns1.03
Hydrophobic ICL2 N 130 34x55 G.hns1 A 39 G.hns1.03
Van-der-waals ICL2 N 130 34x55 G.hns1 A 39 G.hns1.03
Hydrophobic TM5 A 218 5x65 G.H5 L 388 G.H5.20
Van-der-waals TM5 A 218 5x65 G.H5 L 388 G.H5.20
Hydrophobic TM5 I 225 5x72 G.H5 L 394 G.H5.26
Hydrophobic ICL2 L 126 34x51 G.H5 F 376 G.H5.08
Van-der-waals ICL2 L 126 34x51 G.H5 F 376 G.H5.08
Polar H8 N 315 8x47 G.H5 Y 391 G.H5.23
Hydrophobic H8 N 315 8x47 G.H5 Y 391 G.H5.23
Van-der-waals H8 N 315 8x47 G.H5 Y 391 G.H5.23
Ionic TM5 R 230 5x77 G.hgh4 D 323 G.hgh4.13
Polar TM5 R 230 5x77 G.hgh4 D 323 G.hgh4.13
Van-der-waals TM5 R 230 5x77 G.hgh4 D 323 G.hgh4.13
Hydrophobic ICL2 L 126 34x51 G.H5 I 383 G.H5.15
Van-der-waals ICL2 L 126 34x51 G.H5 I 383 G.H5.15
Hydrophobic TM5 T 217 5x64 G.H5 Q 384 G.H5.16
Hydrophobic TM5 I 214 5x61 G.H5 L 388 G.H5.20
Hydrophobic TM5 I 214 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM5 I 214 5x61 G.H5 L 393 G.H5.25
Polar TM6 A 256 6x33 G.H5 L 393 G.H5.25
Hydrophobic TM6 A 256 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM6 A 256 6x33 G.H5 L 393 G.H5.25
Polar TM5 H 221 5x68 G.H5 R 385 G.H5.17
Hydrophobic TM5 H 221 5x68 G.H5 R 385 G.H5.17
Van-der-waals TM5 H 221 5x68 G.H5 R 385 G.H5.17
Hydrophobic TM5 A 231 5x78 G.H4 D 343 G.H4.13
Van-der-waals TM5 A 231 5x78 G.H4 D 343 G.H4.13
Hydrophobic TM5 H 221 5x68 G.H5 L 394 G.H5.26
Hydrophobic TM5 G 232 5x79 G.H4 L 346 G.H4.16
Van-der-waals TM5 G 232 5x79 G.H4 L 346 G.H4.16
Polar TM7 L 314 7x56 G.H5 E 392 G.H5.24
Hydrophobic TM7 L 314 7x56 G.H5 E 392 G.H5.24
Van-der-waals TM7 L 314 7x56 G.H5 E 392 G.H5.24
Polar TM6 T 259 6x36 G.H5 E 392 G.H5.24
Van-der-waals TM6 T 259 6x36 G.H5 E 392 G.H5.24
Hydrophobic H8 K 316 8x48 G.H5 R 389 G.H5.21
Van-der-waals H8 K 316 8x48 G.H5 R 389 G.H5.21
Hydrophobic H8 N 315 8x47 G.H5 Q 390 G.H5.22
Van-der-waals H8 N 315 8x47 G.H5 Q 390 G.H5.22
Hydrophobic ICL2 L 126 34x51 G.H5 R 380 G.H5.12
Van-der-waals ICL2 L 126 34x51 G.H5 R 380 G.H5.12
Polar ICL2 N 130 34x55 G.HN Q 35 G.HN.52
Van-der-waals ICL2 N 130 34x55 G.HN Q 35 G.HN.52
Polar TM5 A 233 5x80 G.H4 R 347 G.H4.17
Van-der-waals TM5 A 233 5x80 G.H4 R 347 G.H4.17
Hydrophobic TM5 L 228 5x75 G.H4 L 346 G.H4.16
Van-der-waals TM5 L 228 5x75 G.H4 L 346 G.H4.16
Hydrophobic TM3 I 121 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 I 121 3x54 G.H5 L 388 G.H5.20
Hydrophobic TM5 H 221 5x68 G.H5 L 388 G.H5.20
Van-der-waals TM5 H 221 5x68 G.H5 L 388 G.H5.20
Aromatic TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM5 H 221 5x68 G.H5 D 381 G.H5.13
Van-der-waals TM5 H 221 5x68 G.H5 D 381 G.H5.13
Polar TM5 H 221 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM5 H 221 5x68 G.H5 Q 384 G.H5.16
Van-der-waals TM5 H 221 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM5 A 231 5x78 G.H4 R 342 G.H4.12
Van-der-waals TM5 A 231 5x78 G.H4 R 342 G.H4.12
Polar TM5 Q 224 5x71 G.H5 R 385 G.H5.17
Van-der-waals TM5 Q 224 5x71 G.H5 R 385 G.H5.17
Hydrophobic ICL2 P 125 34x50 G.H5 Q 384 G.H5.16
Van-der-waals TM3 C 122 3x55 G.H5 Q 384 G.H5.16
Hydrophobic TM5 I 225 5x72 G.H5 R 385 G.H5.17
Van-der-waals TM5 I 225 5x72 G.H5 R 385 G.H5.17
Hydrophobic ICL2 N 130 34x55 G.hns1 R 38 G.hns1.02
Van-der-waals ICL2 N 130 34x55 G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 P 125 34x50 G.H5 R 380 G.H5.12
Hydrophobic TM5 A 222 5x69 G.H5 L 394 G.H5.26
Hydrophobic TM6 T 259 6x36 G.H5 L 393 G.H5.25
Van-der-waals TM6 T 259 6x36 G.H5 L 393 G.H5.25
Hydrophobic TM5 A 231 5x78 G.H4 L 346 G.H4.16
Van-der-waals TM5 A 231 5x78 G.H4 L 346 G.H4.16
Polar TM5 L 228 5x75 G.h4s6 T 350 G.h4s6.03
Van-der-waals TM5 L 228 5x75 G.h4s6 T 350 G.h4s6.03
Hydrophobic TM5 M 227 5x74 G.hgh4 D 323 G.hgh4.13
Van-der-waals TM5 M 227 5x74 G.hgh4 D 323 G.hgh4.13
Hydrophobic TM5 G 232 5x79 G.h4s6 T 350 G.h4s6.03
Hydrophobic TM3 I 121 3x54 G.H5 H 387 G.H5.19
Van-der-waals TM3 I 121 3x54 G.H5 H 387 G.H5.19
Polar ICL2 R 129 34x54 G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 R 129 34x54 G.hns1 R 38 G.hns1.02
Van-der-waals ICL2 R 129 34x54 G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 L 126 34x51 G.H5 C 379 G.H5.11
Van-der-waals ICL2 L 126 34x51 G.H5 C 379 G.H5.11
Van-der-waals TM2 T 54 2x39 G.H5 Y 391 G.H5.23
Ionic H8 K 316 8x48 G.H5 E 392 G.H5.24
Hydrophobic H8 K 316 8x48 G.H5 E 392 G.H5.24
Van-der-waals H8 K 316 8x48 G.H5 E 392 G.H5.24
Polar ICL2 L 126 34x51 G.S1 H 41 G.S1.02
Hydrophobic ICL2 L 126 34x51 G.S1 H 41 G.S1.02
Van-der-waals ICL2 L 126 34x51 G.S1 H 41 G.S1.02
Hydrophobic TM5 L 228 5x75 G.S6 P 361 G.S6.03
Hydrophobic TM6 L 260 6x37 G.H5 L 393 G.H5.25
Ionic H8 R 319 8x51 G.H5 E 392 G.H5.24
Hydrophobic ICL2 L 126 34x51 G.S3 V 217 G.S3.01
Van-der-waals ICL2 L 126 34x51 G.S3 V 217 G.S3.01
Polar TM3 A 120 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 120 3x53 G.H5 H 387 G.H5.19
Polar H8 K 316 8x48 G.H5 Q 390 G.H5.22
Hydrophobic H8 K 316 8x48 G.H5 Q 390 G.H5.22
Van-der-waals H8 K 316 8x48 G.H5 Q 390 G.H5.22