M3 receptor - Gq

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

A

G PROTEIN (FAMILY)

Gq/11

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8E9Z

RESOLUTION

2.7 Å

REFERENCE

10.1038/S41586-022-05489-0

PUBLICATION DATE

Nov. 30, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

31 interactions (Click to see)

LIGANDS

iperoxo - Agonist pubchem gtoplig chembl_ligand

ENDOGENOUS LIGANDS

acetylcholine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 L 174 - G.S3 F 81 G.S3.03
Hydrophobic TM5 T 258 5x65 G.H5 L 240 G.H5.20
Hydrophobic TM5 R 261 5x68 G.H5 L 240 G.H5.20
Hydrophobic TM3 I 170 3x54 G.H5 Y 243 G.H5.23
Hydrophobic TM6 K 488 6x32 G.H5 L 245 G.H5.25
Polar ICL4 C 547 - G.H5 N 244 G.H5.24
Hydrophobic ICL4 C 547 - G.H5 N 244 G.H5.24
Van-der-waals ICL4 C 547 - G.H5 N 244 G.H5.24
Polar TM5 R 261 5x68 G.H5 Q 237 G.H5.17
Hydrophobic TM5 R 261 5x68 G.H5 Q 237 G.H5.17
Van-der-waals TM5 R 261 5x68 G.H5 Q 237 G.H5.17
Polar H8 N 548 8x47 G.H5 E 242 G.H5.22
Hydrophobic H8 N 548 8x47 G.H5 E 242 G.H5.22
Van-der-waals H8 N 548 8x47 G.H5 E 242 G.H5.22
Hydrophobic ICL2 L 174 - G.S3 V 79 G.S3.01
Van-der-waals ICL2 L 174 - G.S3 V 79 G.S3.01
Ionic ICL2 R 177 - G.H5 E 242 G.H5.22
Polar ICL2 R 177 - G.H5 E 242 G.H5.22
Hydrophobic ICL2 R 177 - G.H5 E 242 G.H5.22
Van-der-waals ICL2 R 177 - G.H5 E 242 G.H5.22
Ionic TM5 R 261 5x68 G.H5 D 233 G.H5.13
Hydrophobic TM5 R 261 5x68 G.H5 D 233 G.H5.13
Polar ICL2 R 177 - G.H5 Y 243 G.H5.23
Van-der-waals ICL2 R 177 - G.H5 Y 243 G.H5.23
Hydrophobic ICL4 C 547 - G.H5 Y 243 G.H5.23
Hydrophobic TM6 K 488 6x32 G.H5 N 244 G.H5.24
Van-der-waals TM6 K 488 6x32 G.H5 N 244 G.H5.24
Hydrophobic TM6 K 488 6x32 G.H5 V 246 G.H5.26
Van-der-waals TM6 K 488 6x32 G.H5 V 246 G.H5.26
Hydrophobic ICL2 P 173 - G.H5 K 232 G.H5.12
Van-der-waals ICL2 P 173 - G.H5 K 232 G.H5.12
Hydrophobic ICL2 K 179 - G.hns1 R 32 G.hns1.03
Hydrophobic TM5 I 254 5x61 G.H5 L 245 G.H5.25
Van-der-waals TM5 I 254 5x61 G.H5 L 245 G.H5.25
Hydrophobic TM5 L 265 5x72 G.h4s6 I 210 G.h4s6.20
Hydrophobic TM3 I 170 3x54 G.H5 N 239 G.H5.19
Hydrophobic ICL2 T 175 - G.S3 V 79 G.S3.01
Van-der-waals ICL2 T 175 - G.S3 V 79 G.S3.01
Hydrophobic ICL2 P 173 - G.H5 I 235 G.H5.15
Van-der-waals ICL2 P 173 - G.H5 I 235 G.H5.15
Polar TM2 N 103 2x39 G.H5 Y 243 G.H5.23
Van-der-waals TM2 N 103 2x39 G.H5 Y 243 G.H5.23
Hydrophobic TM6 T 492 6x36 G.H5 L 245 G.H5.25
Van-der-waals TM6 T 492 6x36 G.H5 L 245 G.H5.25
Hydrophobic TM5 R 261 5x68 G.H5 V 246 G.H5.26
Van-der-waals TM5 R 261 5x68 G.H5 V 246 G.H5.26
Hydrophobic ICL2 L 174 - G.H5 K 232 G.H5.12
Polar ICL2 A 178 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 A 178 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 A 178 - G.hns1 R 32 G.hns1.03
Hydrophobic TM5 L 265 5x72 G.H5 D 233 G.H5.13
Van-der-waals TM5 L 265 5x72 G.H5 D 233 G.H5.13
Polar ICL2 R 177 - G.H5 N 239 G.H5.19
Hydrophobic ICL2 R 177 - G.H5 N 239 G.H5.19
Hydrophobic TM6 L 493 6x37 G.H5 L 245 G.H5.25
Hydrophobic TM3 I 170 3x54 G.H5 L 236 G.H5.16
Van-der-waals TM3 I 170 3x54 G.H5 L 236 G.H5.16
Polar TM6 A 489 6x33 G.H5 L 245 G.H5.25
Hydrophobic TM6 A 489 6x33 G.H5 L 245 G.H5.25
Hydrophobic ICL2 L 174 - G.S1 L 34 G.S1.02
Van-der-waals ICL2 L 174 - G.S1 L 34 G.S1.02
Polar TM6 T 492 6x36 G.H5 N 244 G.H5.24
Van-der-waals TM6 T 492 6x36 G.H5 N 244 G.H5.24
Hydrophobic TM5 R 261 5x68 G.H5 L 236 G.H5.16
Aromatic TM3 R 166 3x50 G.H5 Y 243 G.H5.23
Hydrophobic TM3 R 166 3x50 G.H5 Y 243 G.H5.23
Van-der-waals TM3 R 166 3x50 G.H5 Y 243 G.H5.23
Hydrophobic TM3 I 170 3x54 G.H5 L 240 G.H5.20
Van-der-waals TM3 I 170 3x54 G.H5 L 240 G.H5.20
Hydrophobic ICL2 L 174 - G.H5 I 235 G.H5.15
Hydrophobic TM5 T 258 5x65 G.H5 L 245 G.H5.25
Van-der-waals TM5 T 258 5x65 G.H5 L 245 G.H5.25
Van-der-waals H8 R 552 8x51 G.H5 N 244 G.H5.24
Hydrophobic TM5 L 265 5x72 G.S6 Y 212 G.S6.02
Van-der-waals TM5 L 265 5x72 G.S6 Y 212 G.S6.02
Polar TM3 S 169 3x53 G.H5 N 239 G.H5.19
Van-der-waals TM3 S 169 3x53 G.H5 N 239 G.H5.19
Hydrophobic ICL2 L 174 - G.H5 F 228 G.H5.08
Van-der-waals ICL2 L 174 - G.H5 F 228 G.H5.08
Polar TM2 N 103 2x39 G.H5 E 242 G.H5.22
Van-der-waals TM2 N 103 2x39 G.H5 E 242 G.H5.22