EP4 receptor - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8GDA

RESOLUTION

3.3 Å

REFERENCE

10.1073/PNAS.2216329120

PUBLICATION DATE

Jan. 10, 2024

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

38 interactions (Click to see)

LIGANDS

(2S,5R)-5-[(1E,3S)-4,4-difluoro-3-hydroxy-4-phenylbut-1-en-1-yl]-1-[6-(1H-tetrazol-5-yl)hexyl]pyrrolidin-2-ol - Agonist pubchem

PHYSIOLOGICAL LIGANDS

PGF2α, PGI2, PGD2, thromboxane A2, PGE1, PGE2



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Aromatic TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 A 124 34x50 G.H5 I 383 G.H5.15
Hydrophobic ICL2 Y 125 34x51 G.H5 C 379 G.H5.11
Hydrophobic ICL2 S 128 34x54 G.H5 I 383 G.H5.15
Polar TM3 E 116 3x49 G.H5 Y 391 G.H5.23
Hydrophobic TM3 E 116 3x49 G.H5 Y 391 G.H5.23
Van-der-waals TM3 E 116 3x49 G.H5 Y 391 G.H5.23
Polar ICL2 H 129 34x55 G.hns1 R 38 G.hns1.02
Aromatic TM5 F 217 5x72 G.H5 R 385 G.H5.17
Van-der-waals TM5 F 217 5x72 G.H5 R 385 G.H5.17
Hydrophobic ICL1 Q 49 - G.H5 L 394 G.H5.26
Hydrophobic TM2 T 52 2x37 G.H5 Q 390 G.H5.22
Van-der-waals TM2 T 52 2x37 G.H5 Q 390 G.H5.22
Aromatic TM2 F 54 2x39 G.H5 Y 391 G.H5.23
Hydrophobic TM2 F 54 2x39 G.H5 Y 391 G.H5.23
Van-der-waals TM2 F 54 2x39 G.H5 Y 391 G.H5.23
Polar ICL2 V 131 34x57 G.hns1 R 38 G.hns1.02
Van-der-waals ICL2 V 131 34x57 G.hns1 R 38 G.hns1.02
Hydrophobic TM5 M 213 5x68 G.H5 Q 384 G.H5.16
Van-der-waals TM5 M 213 5x68 G.H5 Q 384 G.H5.16
Polar TM5 R 220 5x75 G.S6 Y 360 G.S6.02
Aromatic TM5 R 220 5x75 G.S6 Y 360 G.S6.02
Hydrophobic TM5 R 220 5x75 G.S6 Y 360 G.S6.02
Van-der-waals TM5 R 220 5x75 G.S6 Y 360 G.S6.02
Polar H8 R 333 8x47 G.H5 L 394 G.H5.26
Hydrophobic H8 R 333 8x47 G.H5 L 394 G.H5.26
Van-der-waals H8 R 333 8x47 G.H5 L 394 G.H5.26
Hydrophobic TM2 Y 55 2x40 G.H5 L 394 G.H5.26
Van-der-waals TM2 Y 55 2x40 G.H5 L 394 G.H5.26
Hydrophobic TM6 M 270 6x33 G.H5 L 388 G.H5.20
Polar H8 R 333 8x47 G.H5 Y 391 G.H5.23
Van-der-waals H8 R 333 8x47 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 Y 125 34x51 G.S3 V 217 G.S3.01
Polar TM5 M 213 5x68 G.H5 R 385 G.H5.17
Polar ICL2 Y 125 34x51 G.H5 R 380 G.H5.12
Hydrophobic ICL2 Y 125 34x51 G.H5 R 380 G.H5.12
Van-der-waals ICL2 Y 125 34x51 G.H5 R 380 G.H5.12
Hydrophobic TM5 M 213 5x68 G.H5 L 388 G.H5.20
Hydrophobic TM5 R 220 5x75 G.S6 P 361 G.S6.03
Polar TM3 A 120 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 120 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 A 120 3x53 G.H5 H 387 G.H5.19
Hydrophobic ICL2 A 124 34x50 G.H5 Q 384 G.H5.16
Van-der-waals ICL2 A 124 34x50 G.H5 Q 384 G.H5.16
Polar ICL2 S 128 34x54 G.hns1 R 38 G.hns1.02
Van-der-waals ICL2 S 128 34x54 G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 Y 125 34x51 G.H5 I 383 G.H5.15
Van-der-waals ICL2 Y 125 34x51 G.H5 I 383 G.H5.15
Aromatic ICL2 H 129 34x55 G.S1 H 41 G.S1.02
Hydrophobic ICL2 H 129 34x55 G.S1 H 41 G.S1.02
Van-der-waals ICL2 H 129 34x55 G.S1 H 41 G.S1.02
Polar TM5 R 220 5x75 G.S6 C 359 G.S6.01
Van-der-waals TM5 R 220 5x75 G.S6 C 359 G.S6.01
Hydrophobic ICL2 A 124 34x50 G.H5 H 387 G.H5.19
Polar TM5 R 220 5x75 G.H4 R 342 G.H4.12
Van-der-waals TM5 R 220 5x75 G.H4 R 342 G.H4.12
Hydrophobic TM2 F 54 2x39 G.H5 L 394 G.H5.26
Van-der-waals TM2 F 54 2x39 G.H5 L 394 G.H5.26
Polar TM3 I 121 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 I 121 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 I 121 3x54 G.H5 Q 384 G.H5.16
Polar ICL1 Q 49 - G.H5 L 393 G.H5.25
Hydrophobic ICL1 Q 49 - G.H5 L 393 G.H5.25
Van-der-waals ICL1 Q 49 - G.H5 L 393 G.H5.25
Polar ICL2 Y 127 34x53 G.H5 Q 390 G.H5.22
Polar TM3 E 116 3x49 G.H5 Q 390 G.H5.22
Aromatic ICL2 Y 125 34x51 G.S1 H 41 G.S1.02
Hydrophobic ICL2 Y 125 34x51 G.S1 H 41 G.S1.02
Aromatic ICL2 Y 127 34x53 G.H5 H 387 G.H5.19
Hydrophobic ICL2 Y 127 34x53 G.H5 H 387 G.H5.19
Van-der-waals ICL2 Y 127 34x53 G.H5 H 387 G.H5.19
Hydrophobic ICL3 E 267 - G.H5 L 388 G.H5.20
Ionic ICL3 E 267 - G.H5 R 385 G.H5.17
Polar ICL3 E 267 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 E 267 - G.H5 R 385 G.H5.17
Hydrophobic TM5 L 210 5x65 G.H5 L 388 G.H5.20
Hydrophobic H8 V 336 8x50 G.H5 L 394 G.H5.26
Van-der-waals H8 V 336 8x50 G.H5 L 394 G.H5.26
Hydrophobic TM6 M 270 6x33 G.H5 Y 391 G.H5.23
Hydrophobic TM1 L 42 1x57 G.H5 L 394 G.H5.26
Van-der-waals TM1 L 42 1x57 G.H5 L 394 G.H5.26
Polar ICL2 H 129 34x55 G.HN Q 35 G.HN.52
Hydrophobic ICL2 H 129 34x55 G.hns1 A 39 G.hns1.03
Polar ICL2 Y 125 34x51 G.H5 F 376 G.H5.08
Aromatic ICL2 Y 125 34x51 G.H5 F 376 G.H5.08
Hydrophobic ICL2 Y 125 34x51 G.H5 F 376 G.H5.08
Van-der-waals ICL2 Y 125 34x51 G.H5 F 376 G.H5.08
Hydrophobic TM3 I 121 3x54 G.H5 L 388 G.H5.20