β1-adrenoceptor - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8S2T

RESOLUTION

3.3 Å

REFERENCE

10.1016/J.STR.2025.07.020

PUBLICATION DATE

May 21, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

42 interactions (Click to see)

LIGANDS

LEVISOPRENALINE - Agonist chembl_ligand pubchem drug_central

PHYSIOLOGICAL LIGANDS

(-)-noradrenaline, noradrenaline, (-)-adrenaline



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM3 I 160 3x54 G.H5 Y 377 G.H5.23
Polar TM5 Q 254 5x68 G.H5 D 367 G.H5.13
Hydrophobic TM5 Q 254 5x68 G.H5 D 367 G.H5.13
Van-der-waals TM5 Q 254 5x68 G.H5 D 367 G.H5.13
Polar ICL2 Q 167 - G.S1 H 41 G.S1.02
Hydrophobic TM3 I 160 3x54 G.H5 H 373 G.H5.19
Van-der-waals TM3 I 160 3x54 G.H5 H 373 G.H5.19
Hydrophobic ICL2 Q 167 - G.hns1 A 39 G.hns1.03
Polar TM3 T 161 3x55 G.H5 R 366 G.H5.12
Van-der-waals TM3 T 161 3x55 G.H5 R 366 G.H5.12
Hydrophobic TM3 I 160 3x54 G.H5 L 374 G.H5.20
Van-der-waals TM3 I 160 3x54 G.H5 L 374 G.H5.20
Hydrophobic TM5 V 255 5x69 G.H5 L 380 G.H5.26
Van-der-waals TM5 V 255 5x69 G.H5 L 380 G.H5.26
Hydrophobic ICL2 F 164 - G.S3 F 205 G.S3.03
Ionic TM5 K 257 5x71 G.H5 D 367 G.H5.13
Polar TM5 K 257 5x71 G.H5 D 367 G.H5.13
Hydrophobic TM5 K 257 5x71 G.H5 D 367 G.H5.13
Van-der-waals TM5 K 257 5x71 G.H5 D 367 G.H5.13
Ionic TM5 R 263 5x77 G.hgh4 D 309 G.hgh4.13
Polar TM5 R 263 5x77 G.hgh4 D 309 G.hgh4.13
Van-der-waals TM5 R 263 5x77 G.hgh4 D 309 G.hgh4.13
Ionic ICL3 R 264 - G.hgh4 E 308 G.hgh4.12
Polar ICL3 R 264 - G.hgh4 E 308 G.hgh4.12
Hydrophobic ICL3 R 264 - G.hgh4 E 308 G.hgh4.12
Van-der-waals ICL3 R 264 - G.hgh4 E 308 G.hgh4.12
Hydrophobic ICL3 F 265 - G.H4 R 333 G.H4.17
Van-der-waals ICL3 F 265 - G.H4 R 333 G.H4.17
Polar TM3 I 160 3x54 G.H5 Q 370 G.H5.16
Hydrophobic TM3 I 160 3x54 G.H5 Q 370 G.H5.16
Van-der-waals TM3 I 160 3x54 G.H5 Q 370 G.H5.16
Hydrophobic ICL3 F 265 - G.H4 D 329 G.H4.13
Van-der-waals ICL3 F 265 - G.H4 D 329 G.H4.13
Polar TM3 R 156 3x50 G.H5 Y 377 G.H5.23
Aromatic TM3 R 156 3x50 G.H5 Y 377 G.H5.23
Hydrophobic TM3 R 156 3x50 G.H5 Y 377 G.H5.23
Hydrophobic ICL2 F 164 - G.H5 I 369 G.H5.15
Van-der-waals ICL2 F 164 - G.H5 I 369 G.H5.15
Hydrophobic TM5 I 258 5x72 G.H5 L 380 G.H5.26
Hydrophobic TM6 L 326 6x37 G.H5 L 379 G.H5.25
Van-der-waals TM6 L 326 6x37 G.H5 L 379 G.H5.25
Ionic H8 R 384 8x51 G.H5 E 378 G.H5.24
Polar H8 R 384 8x51 G.H5 E 378 G.H5.24
Van-der-waals H8 R 384 8x51 G.H5 E 378 G.H5.24
Polar TM6 T 325 6x36 G.H5 E 378 G.H5.24
Hydrophobic TM6 T 325 6x36 G.H5 E 378 G.H5.24
Van-der-waals TM6 T 325 6x36 G.H5 E 378 G.H5.24
Hydrophobic ICL2 Y 166 - G.H5 H 373 G.H5.19
Polar TM6 K 321 6x32 G.H5 L 380 G.H5.26
Hydrophobic TM6 K 321 6x32 G.H5 L 380 G.H5.26
Van-der-waals TM6 K 321 6x32 G.H5 L 380 G.H5.26
Hydrophobic TM5 V 247 5x61 G.H5 L 379 G.H5.25
Van-der-waals TM5 V 247 5x61 G.H5 L 379 G.H5.25
Hydrophobic TM5 I 258 5x72 G.h4s6 Y 344 G.h4s6.20
Van-der-waals TM5 I 258 5x72 G.h4s6 Y 344 G.h4s6.20
Polar TM5 Q 254 5x68 G.H5 R 371 G.H5.17
Hydrophobic TM5 Q 254 5x68 G.H5 R 371 G.H5.17
Van-der-waals TM5 Q 254 5x68 G.H5 R 371 G.H5.17
Aromatic ICL2 F 164 - G.H5 F 362 G.H5.08
Hydrophobic ICL2 F 164 - G.H5 F 362 G.H5.08
Van-der-waals ICL2 F 164 - G.H5 F 362 G.H5.08
Hydrophobic ICL3 F 265 - G.h4s6 T 336 G.h4s6.03
Van-der-waals ICL3 F 265 - G.h4s6 T 336 G.h4s6.03
Ionic TM5 E 250 5x64 G.H5 R 366 G.H5.12
Hydrophobic ICL2 F 164 - G.H5 C 365 G.H5.11
Hydrophobic ICL2 F 164 - G.H5 R 366 G.H5.12
Van-der-waals ICL2 F 164 - G.H5 R 366 G.H5.12
Hydrophobic TM5 E 262 5x76 G.h4s6 T 336 G.h4s6.03
Hydrophobic TM5 V 247 5x61 G.H5 L 374 G.H5.20
Polar TM5 E 250 5x64 G.H5 Q 370 G.H5.16
Van-der-waals TM5 E 250 5x64 G.H5 Q 370 G.H5.16
Hydrophobic ICL2 P 163 - G.H5 R 366 G.H5.12
Van-der-waals ICL2 P 163 - G.H5 R 366 G.H5.12
Hydrophobic TM6 T 325 6x36 G.H5 L 379 G.H5.25
Polar TM5 Q 254 5x68 G.H5 Q 370 G.H5.16
Van-der-waals TM5 Q 254 5x68 G.H5 Q 370 G.H5.16
Polar TM2 T 93 2x39 G.H5 Y 377 G.H5.23
Hydrophobic TM2 T 93 2x39 G.H5 Y 377 G.H5.23
Hydrophobic TM5 A 251 5x65 G.H5 L 374 G.H5.20
Hydrophobic TM3 A 159 3x53 G.H5 Y 377 G.H5.23
Van-der-waals TM3 A 159 3x53 G.H5 Y 377 G.H5.23
Hydrophobic TM5 Q 254 5x68 G.H5 L 374 G.H5.20
Van-der-waals TM5 Q 254 5x68 G.H5 L 374 G.H5.20
Polar ICL3 R 264 - G.hgh4 P 307 G.hgh4.11
Hydrophobic ICL3 R 264 - G.hgh4 P 307 G.hgh4.11
Van-der-waals ICL3 R 264 - G.hgh4 P 307 G.hgh4.11
Polar TM6 A 322 6x33 G.H5 L 379 G.H5.25
Hydrophobic TM6 A 322 6x33 G.H5 L 379 G.H5.25
Van-der-waals TM6 A 322 6x33 G.H5 L 379 G.H5.25
Polar ICL2 Q 167 - G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 Q 167 - G.hns1 R 38 G.hns1.02
Van-der-waals ICL2 Q 167 - G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 F 164 - G.S1 H 41 G.S1.02
Van-der-waals ICL2 S 168 - G.hns1 A 39 G.hns1.03
Polar TM3 A 159 3x53 G.H5 H 373 G.H5.19
Hydrophobic TM3 A 159 3x53 G.H5 H 373 G.H5.19
Van-der-waals TM3 A 159 3x53 G.H5 H 373 G.H5.19
Hydrophobic TM5 I 258 5x72 G.H5 R 371 G.H5.17
Van-der-waals TM5 I 258 5x72 G.H5 R 371 G.H5.17
Hydrophobic TM5 C 261 5x75 G.H4 L 332 G.H4.16
Hydrophobic ICL2 F 164 - G.S3 V 203 G.S3.01
Van-der-waals ICL2 F 164 - G.S3 V 203 G.S3.01
Ionic ICL3 R 264 - G.hgh4 D 309 G.hgh4.13
Polar ICL3 R 264 - G.hgh4 D 309 G.hgh4.13
Hydrophobic ICL3 R 264 - G.hgh4 D 309 G.hgh4.13
Van-der-waals ICL3 R 264 - G.hgh4 D 309 G.hgh4.13
Hydrophobic ICL3 F 265 - G.H4 L 332 G.H4.16
Van-der-waals ICL3 F 265 - G.H4 L 332 G.H4.16
Hydrophobic ICL2 P 163 - G.H5 I 369 G.H5.15
Van-der-waals ICL2 P 163 - G.H5 I 369 G.H5.15
Hydrophobic ICL2 P 163 - G.H5 Q 370 G.H5.16