GPR4 - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Mus musculus

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8ZF9

RESOLUTION

2.6 Å

REFERENCE

10.1016/J.CELL.2024.12.001

PUBLICATION DATE

Feb. 26, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

0 interactions (Click to see)

LIGANDS

Apo (no ligand) -

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 L 125 - G.S3 V 227 G.S3.03
Polar TM3 A 120 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 120 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 A 120 3x53 G.H5 H 387 G.H5.19
Polar ICL3 V 214 - G.H5 D 381 G.H5.13
Van-der-waals ICL3 V 214 - G.H5 D 381 G.H5.13
Hydrophobic TM2 L 54 2x39 G.H5 E 392 G.H5.24
Hydrophobic ICL2 R 131 - G.H5 H 387 G.H5.19
Van-der-waals ICL2 R 131 - G.H5 H 387 G.H5.19
Hydrophobic TM5 I 206 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM5 I 206 5x61 G.H5 L 393 G.H5.25
Polar TM2 N 52 2x37 G.H5 Q 390 G.H5.22
Hydrophobic TM2 N 52 2x37 G.H5 Q 390 G.H5.22
Polar ICL2 R 131 - G.H5 Y 391 G.H5.23
Hydrophobic ICL2 R 131 - G.H5 Y 391 G.H5.23
Van-der-waals ICL2 R 131 - G.H5 Y 391 G.H5.23
Polar ICL3 S 215 - G.H5 D 381 G.H5.13
Van-der-waals ICL3 S 215 - G.H5 D 381 G.H5.13
Polar TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 117 3x50 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 L 125 - G.H5 I 383 G.H5.15
Polar ICL3 S 215 - G.H5 R 385 G.H5.17
Hydrophobic ICL3 S 215 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 S 215 - G.H5 R 385 G.H5.17
Polar TM5 A 209 5x64 G.H5 Q 384 G.H5.16
Hydrophobic TM6 I 224 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM6 I 224 6x33 G.H5 L 393 G.H5.25
Hydrophobic ICL2 P 124 - G.H5 R 380 G.H5.12
Hydrophobic TM3 D 116 3x49 G.H5 Y 391 G.H5.23
Van-der-waals TM3 D 116 3x49 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 P 124 - G.H5 I 383 G.H5.15
Hydrophobic TM5 Y 203 5x58 G.H5 L 393 G.H5.25
Polar ICL3 S 215 - G.S6 Y 360 G.S6.02
Hydrophobic ICL2 L 125 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 L 125 - G.H5 F 376 G.H5.08
Van-der-waals TM4 R 132 4x38 G.HN Q 35 G.HN.52
Polar ICL3 S 213 - G.H5 Q 384 G.H5.16
Van-der-waals ICL3 S 213 - G.H5 Q 384 G.H5.16
Hydrophobic ICL3 S 215 - G.h4s6 Y 358 G.h4s6.20
Hydrophobic TM6 I 224 6x33 G.H5 L 394 G.H5.26
Polar ICL3 T 216 - G.H5 R 385 G.H5.17
Polar ICL2 A 128 - G.hns1 R 38 G.hns1.02
Hydrophobic TM2 L 54 2x39 G.H5 Y 391 G.H5.23
Hydrophobic TM3 V 121 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 V 121 3x54 G.H5 L 388 G.H5.20
Polar ICL2 R 126 - G.S3 D 225 G.S3.01
Hydrophobic ICL2 R 126 - G.S3 D 225 G.S3.01
Van-der-waals ICL2 R 126 - G.S3 D 225 G.S3.01
Hydrophobic ICL2 L 125 - G.S1 H 41 G.S1.02
Hydrophobic TM5 V 210 5x65 G.H5 L 388 G.H5.20
Hydrophobic ICL3 V 214 - G.S6 Y 360 G.S6.02
Hydrophobic TM3 V 121 3x54 G.H5 L 393 G.H5.25
Van-der-waals TM3 V 121 3x54 G.H5 L 393 G.H5.25
Polar H8 N 292 8x47 G.H5 E 392 G.H5.24
Hydrophobic H8 N 292 8x47 G.H5 E 392 G.H5.24
Van-der-waals H8 N 292 8x47 G.H5 E 392 G.H5.24
Hydrophobic TM3 V 121 3x54 G.H5 H 387 G.H5.19
Polar TM3 V 121 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 V 121 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 V 121 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM6 I 224 6x33 G.H5 L 388 G.H5.20
Hydrophobic TM6 L 227 6x36 G.H5 L 393 G.H5.25
Polar TM2 E 53 2x38 G.H5 Y 391 G.H5.23
Hydrophobic TM2 E 53 2x38 G.H5 Y 391 G.H5.23
Van-der-waals TM2 E 53 2x38 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 R 126 - G.S3 V 227 G.S3.03
Hydrophobic ICL2 L 125 - G.H5 R 380 G.H5.12
Polar ICL3 V 214 - G.h4s6 Y 358 G.h4s6.20
Hydrophobic ICL3 V 214 - G.h4s6 Y 358 G.h4s6.20
Ionic TM6 E 220 6x29 G.H5 R 385 G.H5.17
Polar TM6 E 220 6x29 G.H5 R 385 G.H5.17
Hydrophobic TM6 E 220 6x29 G.H5 R 385 G.H5.17
Van-der-waals TM6 E 220 6x29 G.H5 R 385 G.H5.17
Polar TM3 A 122 3x55 G.H5 R 380 G.H5.12
Van-der-waals TM3 A 122 3x55 G.H5 R 380 G.H5.12
Hydrophobic ICL2 P 124 - G.H5 Q 384 G.H5.16
Van-der-waals ICL3 S 213 - G.H5 D 381 G.H5.13
Hydrophobic ICL2 P 124 - G.H5 H 387 G.H5.19
Hydrophobic TM3 R 117 3x50 G.H5 L 393 G.H5.25
Hydrophobic TM3 A 120 3x53 G.H5 Y 391 G.H5.23
Van-der-waals TM3 A 120 3x53 G.H5 Y 391 G.H5.23
Hydrophobic TM6 E 220 6x29 G.H5 L 394 G.H5.26