H4 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9LRE

RESOLUTION

2.8 Å

REFERENCE

10.1038/S42003-025-08363-7

PUBLICATION DATE

June 11, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

21 interactions (Click to see)

LIGANDS

histamine - Agonist pubchem gtoplig chembl_ligand drug_central drugbank pubchem

PHYSIOLOGICAL LIGANDS

CCL16 (Human), histamine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM7 C 361 7x56 G.H5 C 351 G.H5.23
Polar TM3 S 115 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 S 115 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 S 115 3x53 G.H5 N 347 G.H5.19
Polar TM6 R 300 6x32 G.H5 F 354 G.H5.26
Hydrophobic TM6 R 300 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 R 300 6x32 G.H5 F 354 G.H5.26
Ionic H8 R 364 8x49 G.H5 D 350 G.H5.22
Polar H8 R 364 8x49 G.H5 D 350 G.H5.22
Hydrophobic H8 R 364 8x49 G.H5 D 350 G.H5.22
Van-der-waals H8 R 364 8x49 G.H5 D 350 G.H5.22
Polar ICL2 T 124 34x55 G.s2s3 D 193 G.s2s3.02
Ionic TM5 D 205 5x69 G.H5 K 345 G.H5.17
Polar TM5 D 205 5x69 G.H5 K 345 G.H5.17
Hydrophobic H8 K 363 8x48 G.H5 G 352 G.H5.24
Hydrophobic H8 H 362 8x47 G.H5 C 351 G.H5.23
Hydrophobic TM6 L 301 6x33 G.H5 L 348 G.H5.20
Hydrophobic TM5 R 204 5x68 G.H5 I 344 G.H5.16
Van-der-waals TM5 R 204 5x68 G.H5 I 344 G.H5.16
Hydrophobic TM3 R 112 3x50 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 116 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 V 116 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 R 123 34x54 G.H5 I 343 G.H5.15
Hydrophobic ICL2 A 119 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 A 119 34x50 G.H5 I 344 G.H5.16
Ionic TM6 R 297 6x29 G.h4s6 D 315 G.h4s6.09
Polar TM6 R 297 6x29 G.h4s6 D 315 G.h4s6.09
Hydrophobic TM6 R 297 6x29 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 R 297 6x29 G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL2 H 126 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 H 126 - G.hns1 R 32 G.hns1.03
Hydrophobic TM3 V 116 3x54 G.H5 I 344 G.H5.16
Hydrophobic TM6 V 293 6x25 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 V 293 6x25 G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL2 V 120 34x51 G.H5 F 336 G.H5.08
Van-der-waals ICL2 V 120 34x51 G.H5 F 336 G.H5.08
Hydrophobic TM6 L 301 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 301 6x33 G.H5 L 353 G.H5.25
Polar H8 H 362 8x47 G.H5 D 350 G.H5.22
Hydrophobic H8 H 362 8x47 G.H5 D 350 G.H5.22
Van-der-waals H8 H 362 8x47 G.H5 D 350 G.H5.22
Ionic TM6 R 300 6x32 G.h4s6 D 315 G.h4s6.09
Polar TM6 R 300 6x32 G.h4s6 D 315 G.h4s6.09
Polar ICL2 R 123 34x54 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 R 123 34x54 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 R 123 34x54 G.hns1 R 32 G.hns1.03
Van-der-waals TM6 R 290 6x22 G.S6 I 319 G.S6.01
Hydrophobic TM5 I 197 5x61 G.H5 L 348 G.H5.20
Van-der-waals TM5 I 197 5x61 G.H5 L 348 G.H5.20
Hydrophobic TM5 L 201 5x65 G.H5 F 354 G.H5.26
Van-der-waals TM5 L 201 5x65 G.H5 F 354 G.H5.26
Hydrophobic ICL2 R 123 34x54 G.S3 L 194 G.S3.01
Polar TM7 C 361 7x56 G.H5 G 352 G.H5.24
Hydrophobic TM7 C 361 7x56 G.H5 G 352 G.H5.24
Van-der-waals TM7 C 361 7x56 G.H5 G 352 G.H5.24
Polar ICL2 R 123 34x54 G.hns1 A 31 G.hns1.02
Hydrophobic ICL2 A 119 34x50 G.H5 I 343 G.H5.15
Ionic TM6 R 290 6x22 G.H4 E 308 G.H4.16
Hydrophobic ICL2 T 124 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals TM5 L 201 5x65 G.H5 K 345 G.H5.17
Hydrophobic TM6 L 305 6x37 G.H5 L 353 G.H5.25
Polar TM6 S 304 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 S 304 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 S 304 6x36 G.H5 L 353 G.H5.25
Polar ICL2 R 123 34x54 G.S1 E 33 G.S1.01
Hydrophobic ICL2 V 120 34x51 G.s2s3 D 193 G.s2s3.02
Ionic TM6 R 290 6x22 G.h4s6 E 318 G.h4s6.12
Polar TM6 R 290 6x22 G.h4s6 E 318 G.h4s6.12
Van-der-waals TM6 R 290 6x22 G.h4s6 E 318 G.h4s6.12
Polar TM6 R 290 6x22 G.h4s6 K 317 G.h4s6.11
Hydrophobic TM6 R 297 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM6 L 308 6x40 G.H5 L 353 G.H5.25
Hydrophobic ICL2 V 120 34x51 G.S3 L 194 G.S3.01
Hydrophobic TM3 R 112 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 112 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM6 L 301 6x33 G.H5 F 354 G.H5.26
Hydrophobic TM6 R 297 6x29 G.H5 K 345 G.H5.17
Van-der-waals TM6 R 297 6x29 G.H5 K 345 G.H5.17
Polar H8 K 363 8x48 G.H5 D 350 G.H5.22
Hydrophobic H8 K 363 8x48 G.H5 D 350 G.H5.22
Van-der-waals H8 K 363 8x48 G.H5 D 350 G.H5.22
Ionic TM5 R 204 5x68 G.H5 D 341 G.H5.13
Polar TM5 R 204 5x68 G.H5 D 341 G.H5.13
Hydrophobic TM5 R 204 5x68 G.H5 D 341 G.H5.13
Van-der-waals TM5 R 204 5x68 G.H5 D 341 G.H5.13
Ionic TM6 R 297 6x29 G.h4s6 E 318 G.h4s6.12
Polar TM6 R 297 6x29 G.h4s6 E 318 G.h4s6.12
Van-der-waals TM6 R 297 6x29 G.h4s6 E 318 G.h4s6.12