M4 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9N29

RESOLUTION

2.6 Å

REFERENCE

10.1038/S41392-026-02865-4

PUBLICATION DATE

July 15, 2026

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

44 interactions (Click to see)

LIGANDS

diphenhydramine - Agonist pubchem gtoplig chembl_ligand drugbank drug_central chembl_ligand pubchem pubchem drugbank drugbank pubchem pubchem pubchem pubchem

PHYSIOLOGICAL LIGANDS

acetylcholine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 A 142 - G.hns1 A 26 G.hns1.02
Hydrophobic TM6 I 402 6x37 G.H5 L 240 G.H5.25
Van-der-waals TM6 I 402 6x37 G.H5 L 240 G.H5.25
Polar H8 N 457 8x47 G.H5 R 236 G.H5.21
Hydrophobic TM3 V 134 3x54 G.H5 L 235 G.H5.20
Hydrophobic ICL2 P 137 - G.H5 I 231 G.H5.16
Van-der-waals ICL2 P 137 - G.H5 I 231 G.H5.16
Hydrophobic TM6 R 394 6x29 G.H5 F 241 G.H5.26
Van-der-waals TM6 R 394 6x29 G.H5 F 241 G.H5.26
Hydrophobic ICL2 P 137 - G.H5 T 227 G.H5.12
Van-der-waals ICL2 P 137 - G.H5 T 227 G.H5.12
Hydrophobic TM6 V 398 6x33 G.H5 F 241 G.H5.26
Van-der-waals TM6 V 398 6x33 G.H5 F 241 G.H5.26
Polar TM2 N 67 2x39 G.H5 D 237 G.H5.22
Hydrophobic TM2 N 67 2x39 G.H5 D 237 G.H5.22
Van-der-waals TM2 N 67 2x39 G.H5 D 237 G.H5.22
Hydrophobic ICL2 L 138 - G.S3 V 74 G.S3.01
Hydrophobic ICL2 L 138 - G.H5 F 223 G.H5.08
Van-der-waals ICL2 L 138 - G.H5 F 223 G.H5.08
Polar H8 N 457 8x47 G.H5 D 237 G.H5.22
Van-der-waals H8 N 457 8x47 G.H5 D 237 G.H5.22
Hydrophobic ICL2 T 139 - G.S3 V 74 G.S3.01
Hydrophobic TM6 T 401 6x36 G.H5 L 240 G.H5.25
Polar ICL3 S 224 - G.H5 D 228 G.H5.13
Hydrophobic ICL3 S 224 - G.H5 D 228 G.H5.13
Van-der-waals ICL3 S 224 - G.H5 D 228 G.H5.13
Hydrophobic ICL2 L 138 - G.H5 T 227 G.H5.12
Hydrophobic ICL2 L 138 - G.S3 F 76 G.S3.03
Hydrophobic TM6 V 398 6x33 G.H5 L 240 G.H5.25
Van-der-waals TM6 V 398 6x33 G.H5 L 240 G.H5.25
Polar TM3 R 130 3x50 G.H5 C 238 G.H5.23
Hydrophobic TM3 R 130 3x50 G.H5 C 238 G.H5.23
Van-der-waals TM3 R 130 3x50 G.H5 C 238 G.H5.23
Hydrophobic TM4 T 145 4x38 G.HN E 23 G.HN.52
Van-der-waals TM4 T 145 4x38 G.HN E 23 G.HN.52
Hydrophobic H8 N 457 8x47 G.H5 G 239 G.H5.24
Hydrophobic TM3 V 134 3x54 G.H5 I 231 G.H5.16
Van-der-waals TM3 V 134 3x54 G.H5 I 231 G.H5.16
Hydrophobic TM3 C 133 3x53 G.H5 N 234 G.H5.19
Van-der-waals TM3 C 133 3x53 G.H5 N 234 G.H5.19
Hydrophobic ICL2 A 142 - G.hns1 R 27 G.hns1.03
Van-der-waals ICL2 A 142 - G.hns1 R 27 G.hns1.03
Hydrophobic ICL2 L 138 - G.H5 I 230 G.H5.15
Van-der-waals ICL2 L 138 - G.H5 I 230 G.H5.15
Hydrophobic TM7 C 456 7x56 G.H5 G 239 G.H5.24
Hydrophobic ICL2 L 138 - G.H5 V 226 G.H5.11
Hydrophobic TM2 N 67 2x39 G.H5 C 238 G.H5.23
Hydrophobic ICL2 P 137 - G.H5 I 230 G.H5.15
Van-der-waals ICL2 P 137 - G.H5 I 230 G.H5.15
Hydrophobic TM3 R 130 3x50 G.H5 L 240 G.H5.25
Polar TM2 T 65 2x37 G.H5 D 237 G.H5.22