PAC1 receptor - Gs

GPCR CLASS (FAMILY)

Class B1 (Secretin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9P94

RESOLUTION

3.0 Å

REFERENCE

10.1073/PNAS.2521157122

PUBLICATION DATE

Nov. 19, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

88 interactions (Click to see)

LIGANDS

PACAP-27 (Human) - Agonist pubchem gtoplig uniprot uniprot uniprot uniprot pubchem

PHYSIOLOGICAL LIGANDS

PHV (Rat), VIP (Human), PHV (Human), PHM (Human), PHI (Mouse), PACAP-38 (Human), PACAP-27 (Human)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Aromatic TM2 R 185 2x46 G.H5 Y 391 G.H5.23
Hydrophobic TM2 R 185 2x46 G.H5 Y 391 G.H5.23
Van-der-waals TM2 R 185 2x46 G.H5 Y 391 G.H5.23
Hydrophobic TM5 K 334 5x64 G.H5 L 388 G.H5.20
Hydrophobic TM5 K 334 5x64 G.H5 L 394 G.H5.26
Hydrophobic ICL2 T 258 - G.H5 I 383 G.H5.15
Aromatic ICL2 F 259 - G.S1 H 41 G.S1.02
Hydrophobic ICL2 F 259 - G.S1 H 41 G.S1.02
Van-der-waals ICL2 F 259 - G.S1 H 41 G.S1.02
Hydrophobic TM6 R 350 6x37 G.H5 L 394 G.H5.26
Van-der-waals TM6 R 350 6x37 G.H5 L 394 G.H5.26
Polar TM6 R 350 6x37 G.H5 L 393 G.H5.25
Van-der-waals TM6 R 350 6x37 G.H5 L 393 G.H5.25
Polar TM6 R 350 6x37 G.H5 E 392 G.H5.24
Hydrophobic TM3 L 251 3x54 G.H5 L 393 G.H5.25
Hydrophobic ICL2 T 258 - G.H5 Q 384 G.H5.16
Van-der-waals ICL2 T 258 - G.H5 Q 384 G.H5.16
Hydrophobic TM2 H 189 2x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 Y 250 3x53 G.H5 Y 391 G.H5.23
Ionic TM5 K 334 5x64 G.H5 D 381 G.H5.13
Polar TM5 K 334 5x64 G.H5 D 381 G.H5.13
Hydrophobic TM3 L 251 3x54 G.H5 Y 391 G.H5.23
Van-der-waals TM3 L 251 3x54 G.H5 Y 391 G.H5.23
Hydrophobic TM3 L 255 3x58 G.H5 H 387 G.H5.19
Hydrophobic ICL2 F 259 - G.S3 F 219 G.S3.03
Hydrophobic TM5 L 331 5x61 G.H5 L 393 G.H5.25
Hydrophobic TM5 L 331 5x61 G.H5 L 394 G.H5.26
Van-der-waals TM5 L 331 5x61 G.H5 L 394 G.H5.26
Hydrophobic TM6 L 358 6x45 G.H5 L 393 G.H5.25
Hydrophobic TM3 L 254 3x57 G.H5 H 387 G.H5.19
Hydrophobic TM5 L 335 5x65 G.H5 L 394 G.H5.26
Polar TM6 R 353 6x40 G.H5 E 392 G.H5.24
Hydrophobic TM5 I 327 5x57 G.H5 L 393 G.H5.25
Hydrophobic ICL2 F 259 - G.S3 V 217 G.S3.01
Hydrophobic TM3 L 254 3x57 G.H5 Y 391 G.H5.23
Polar ICL3 S 337 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 S 337 - G.H5 R 385 G.H5.17
Hydrophobic ICL2 F 259 - G.H5 C 379 G.H5.11
Hydrophobic ICL2 F 259 - G.H5 R 380 G.H5.12
Hydrophobic TM5 L 331 5x61 G.H5 L 388 G.H5.20
Van-der-waals TM5 L 331 5x61 G.H5 L 388 G.H5.20
Polar TM5 K 334 5x64 G.H5 R 385 G.H5.17
Van-der-waals TM5 K 334 5x64 G.H5 R 385 G.H5.17
Hydrophobic TM3 L 255 3x58 G.H5 L 388 G.H5.20
Van-der-waals TM3 L 255 3x58 G.H5 L 388 G.H5.20
Hydrophobic ICL2 F 260 - G.S3 V 217 G.S3.01
Hydrophobic ICL2 F 259 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 F 259 - G.H5 I 383 G.H5.15
Polar TM2 R 185 2x46 G.H5 Q 390 G.H5.22
Hydrophobic TM2 R 185 2x46 G.H5 Q 390 G.H5.22
Van-der-waals TM2 R 185 2x46 G.H5 Q 390 G.H5.22
Polar TM3 L 255 3x58 G.H5 Q 384 G.H5.16
Van-der-waals TM3 L 255 3x58 G.H5 Q 384 G.H5.16
Aromatic ICL2 F 259 - G.H5 F 376 G.H5.08
Hydrophobic ICL2 F 259 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 F 259 - G.H5 F 376 G.H5.08
Polar TM3 V 256 3x59 G.H5 R 380 G.H5.12
Polar TM5 K 334 5x64 G.H5 Q 384 G.H5.16
Hydrophobic TM5 K 334 5x64 G.H5 Q 384 G.H5.16
Van-der-waals TM5 K 334 5x64 G.H5 Q 384 G.H5.16