NTS1 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9VB3

RESOLUTION

3.0 Å

REFERENCE

10.1038/S41586-026-10228-W

PUBLICATION DATE

June 3, 2026

3D VIEW

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LIGAND INTERACTIONS

50 interactions (Click to see)

LIGANDS

JMV449 - Agonist pubchem gtoplig

PHYSIOLOGICAL LIGANDS

large neuromedin N (Mouse), neurotensin (Human), neuromedin N (Human), large neuromedin N (Human), large neuromedin N (Rat), large neurotensin (Human), neuromedin N (Mouse)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM6 G 293 6x25 G.h4s6 T 316 G.h4s6.10
Hydrophobic ICL4 V 367 - G.H5 F 354 G.H5.26
Van-der-waals ICL4 V 367 - G.H5 F 354 G.H5.26
Hydrophobic TM3 I 170 3x54 G.H5 L 353 G.H5.25
Van-der-waals TM3 I 170 3x54 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 173 34x50 G.H5 L 348 G.H5.20
Hydrophobic TM7 Y 364 7x53 G.H5 G 352 G.H5.24
Van-der-waals TM7 Y 364 7x53 G.H5 G 352 G.H5.24
Van-der-waals ICL2 F 174 34x51 G.H5 K 345 G.H5.17
Hydrophobic TM6 P 292 6x24 G.h4s6 K 317 G.h4s6.11
Van-der-waals TM6 P 292 6x24 G.h4s6 K 317 G.h4s6.11
Polar TM6 R 294 6x26 G.H5 N 346 G.H5.18
Van-der-waals TM6 R 294 6x26 G.H5 N 346 G.H5.18
Ionic TM3 R 166 3x50 G.H5 D 350 G.H5.22
Hydrophobic TM3 R 166 3x50 G.H5 D 350 G.H5.22
Van-der-waals TM3 R 166 3x50 G.H5 D 350 G.H5.22
Hydrophobic TM6 V 304 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 V 304 6x36 G.H5 L 353 G.H5.25
Hydrophobic ICL2 K 177 34x54 G.S3 L 194 G.S3.01
Van-der-waals ICL2 K 177 34x54 G.S3 L 194 G.S3.01
Polar TM4 S 183 4x40 G.HN E 28 G.HN.52
Polar ICL3 S 287 - G.S6 I 319 G.S6.01
Van-der-waals ICL3 S 287 - G.S6 I 319 G.S6.01
Hydrophobic ICL3 S 287 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 S 287 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 I 290 - G.H5 N 346 G.H5.18
Hydrophobic ICL4 V 367 - G.H5 G 352 G.H5.24
Van-der-waals ICL4 V 367 - G.H5 G 352 G.H5.24
Hydrophobic ICL2 P 173 34x50 G.H5 K 345 G.H5.17
Polar TM6 G 293 6x25 G.h4s6 D 315 G.h4s6.09
Hydrophobic TM6 G 293 6x25 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 G 293 6x25 G.h4s6 D 315 G.h4s6.09
Polar ICL3 E 291 - G.H5 N 346 G.H5.18
Van-der-waals ICL3 E 291 - G.H5 N 346 G.H5.18
Hydrophobic ICL2 K 177 34x54 G.H5 I 344 G.H5.16
Hydrophobic TM6 P 292 6x24 G.h4s6 T 316 G.h4s6.10
Polar TM4 S 181 4x38 G.HN E 28 G.HN.52
Van-der-waals TM4 S 181 4x38 G.HN E 28 G.HN.52
Hydrophobic TM6 L 305 6x37 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 305 6x37 G.H5 L 353 G.H5.25
Polar ICL2 K 177 34x54 G.hns1 A 31 G.hns1.02
Polar ICL1 Q 98 - G.hns1 A 31 G.hns1.02
Van-der-waals ICL1 Q 98 - G.hns1 A 31 G.hns1.02
Hydrophobic TM3 I 170 3x54 G.H5 L 348 G.H5.20
Polar ICL3 A 289 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 A 289 - G.S6 Y 320 G.S6.02
Polar ICL3 S 284 - G.S6 H 322 G.S6.04
Hydrophobic ICL3 S 284 - G.S6 H 322 G.S6.04
Van-der-waals ICL3 S 284 - G.S6 H 322 G.S6.04
Ionic TM6 R 294 6x26 G.h3s5 D 261 G.h3s5.02
Polar TM6 R 294 6x26 G.h3s5 D 261 G.h3s5.02
Hydrophobic TM2 L 105 2x43 G.H5 C 351 G.H5.23
Hydrophobic TM6 P 292 6x24 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 P 292 6x24 G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL3 C 277 - G.H4 Q 304 G.H4.12
Hydrophobic ICL2 T 178 34x55 G.s2s3 D 193 G.s2s3.02
Van-der-waals ICL2 T 178 34x55 G.s2s3 D 193 G.s2s3.02
Hydrophobic ICL2 F 174 34x51 G.H5 T 340 G.H5.12
Van-der-waals ICL2 F 174 34x51 G.H5 T 340 G.H5.12
Polar ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 F 174 34x51 G.H5 I 344 G.H5.16
Van-der-waals ICL2 F 174 34x51 G.H5 I 344 G.H5.16
Hydrophobic ICL2 P 173 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 173 34x50 G.H5 I 344 G.H5.16
Hydrophobic ICL3 C 277 - G.H4 C 305 G.H4.13
Van-der-waals ICL3 C 277 - G.H4 C 305 G.H4.13
Hydrophobic TM6 P 292 6x24 G.h4s6 E 318 G.h4s6.12
Van-der-waals TM6 P 292 6x24 G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL1 L 94 - G.h2s4 E 216 G.h2s4.02
Hydrophobic ICL1 V 101 - G.H5 D 350 G.H5.22
Van-der-waals ICL1 V 101 - G.H5 D 350 G.H5.22
Hydrophobic TM3 A 169 3x53 G.H5 L 348 G.H5.20
Van-der-waals TM3 A 169 3x53 G.H5 L 348 G.H5.20
Polar ICL3 Q 275 - G.H4 Q 304 G.H4.12
Hydrophobic ICL3 F 286 - G.S6 H 322 G.S6.04
Hydrophobic ICL3 F 286 - G.S6 T 321 G.S6.03
Van-der-waals ICL3 F 286 - G.S6 T 321 G.S6.03
Hydrophobic TM6 L 298 6x30 G.H5 F 354 G.H5.26
Van-der-waals TM6 L 298 6x30 G.H5 F 354 G.H5.26
Hydrophobic TM3 R 166 3x50 G.H5 L 353 G.H5.25
Hydrophobic TM5 L 263 5x65 G.H5 F 354 G.H5.26
Polar ICL3 S 287 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 S 287 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 S 287 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 H 283 - G.S6 F 323 G.S6.05
Hydrophobic ICL3 S 284 - G.H5 F 334 G.H5.06
Hydrophobic ICL3 I 290 - G.H5 K 345 G.H5.17
Hydrophobic ICL2 T 178 34x55 G.S3 L 194 G.S3.01
Van-der-waals ICL3 Q 275 - G.S6 T 321 G.S6.03
Hydrophobic ICL3 E 282 - G.S6 F 323 G.S6.05
Van-der-waals ICL3 E 282 - G.S6 F 323 G.S6.05
Polar ICL3 E 291 - G.S6 Y 320 G.S6.02
Hydrophobic ICL1 L 97 - G.hns1 A 31 G.hns1.02
Polar ICL3 E 282 - G.H4 Y 296 G.H4.03
Hydrophobic ICL3 E 282 - G.H4 Y 296 G.H4.03
Van-der-waals ICL3 E 282 - G.H4 Y 296 G.H4.03
Polar TM6 G 301 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM6 G 301 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 G 301 6x33 G.H5 L 353 G.H5.25
Hydrophobic ICL4 V 367 - G.H5 L 353 G.H5.25
Polar ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 K 177 34x54 G.S1 V 34 G.S1.02
Hydrophobic TM3 I 170 3x54 G.H5 F 354 G.H5.26
Ionic ICL3 E 291 - G.h4s6 K 317 G.h4s6.11
Polar ICL3 E 291 - G.h4s6 K 317 G.h4s6.11
Hydrophobic ICL3 E 291 - G.h4s6 K 317 G.h4s6.11
Van-der-waals ICL3 E 291 - G.h4s6 K 317 G.h4s6.11
Hydrophobic ICL2 F 174 34x51 G.s2s3 K 192 G.s2s3.01
Hydrophobic TM6 A 297 6x29 G.H5 F 354 G.H5.26
Hydrophobic ICL3 F 286 - G.S6 Y 320 G.S6.02
Polar ICL2 K 177 34x54 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 K 177 34x54 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 F 174 34x51 G.H5 D 341 G.H5.13
Hydrophobic ICL2 F 174 34x51 G.S3 L 194 G.S3.01
Van-der-waals ICL2 F 174 34x51 G.S3 L 194 G.S3.01
Polar ICL1 S 99 - G.H5 K 349 G.H5.21
Van-der-waals ICL1 S 99 - G.H5 K 349 G.H5.21
Hydrophobic TM5 M 266 5x68 G.H5 L 348 G.H5.20