NTS1 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9VB4

RESOLUTION

2.9 Å

REFERENCE

10.1038/S41586-026-10228-W

PUBLICATION DATE

June 3, 2026

3D VIEW

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LIGAND INTERACTIONS

55 interactions (Click to see)

LIGANDS

JMV449 - Agonist pubchem gtoplig

PHYSIOLOGICAL LIGANDS

large neuromedin N (Mouse), neurotensin (Human), neuromedin N (Human), large neuromedin N (Human), large neuromedin N (Rat), large neurotensin (Human), neuromedin N (Mouse)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM2 L 105 2x43 G.H5 C 351 G.H5.23
Hydrophobic TM1 L 88 1x57 G.H5 C 351 G.H5.23
Polar TM6 R 294 6x26 G.h4s6 D 315 G.h4s6.09
Hydrophobic TM6 R 294 6x26 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 R 294 6x26 G.h4s6 D 315 G.h4s6.09
Polar H8 N 370 8x49 G.H5 C 351 G.H5.23
Hydrophobic H8 N 370 8x49 G.H5 C 351 G.H5.23
Van-der-waals H8 N 370 8x49 G.H5 C 351 G.H5.23
Hydrophobic TM3 I 170 3x54 G.H5 L 353 G.H5.25
Van-der-waals TM3 I 170 3x54 G.H5 L 353 G.H5.25
Hydrophobic TM5 L 263 5x65 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 173 34x50 G.H5 L 348 G.H5.20
Polar ICL2 T 178 34x55 G.s2s3 D 193 G.s2s3.02
Van-der-waals ICL2 T 178 34x55 G.s2s3 D 193 G.s2s3.02
Hydrophobic ICL2 F 174 34x51 G.H5 T 340 G.H5.12
Hydrophobic ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 T 178 34x55 G.hns1 R 32 G.hns1.03
Polar ICL4 S 368 - G.H5 L 353 G.H5.25
Hydrophobic ICL4 S 368 - G.H5 L 353 G.H5.25
Van-der-waals ICL4 S 368 - G.H5 L 353 G.H5.25
Hydrophobic TM7 Y 364 7x53 G.H5 G 352 G.H5.24
Van-der-waals TM7 Y 364 7x53 G.H5 G 352 G.H5.24
Van-der-waals ICL2 F 174 34x51 G.H5 K 345 G.H5.17
Polar ICL4 S 368 - G.H5 F 354 G.H5.26
Hydrophobic ICL4 S 368 - G.H5 F 354 G.H5.26
Van-der-waals ICL4 S 368 - G.H5 F 354 G.H5.26
Polar ICL3 E 291 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 E 291 - G.S6 Y 320 G.S6.02
Hydrophobic ICL2 F 174 34x51 G.H5 I 344 G.H5.16
Van-der-waals ICL2 F 174 34x51 G.H5 I 344 G.H5.16
Hydrophobic ICL1 L 97 - G.hns1 A 31 G.hns1.02
Van-der-waals ICL1 L 97 - G.hns1 A 31 G.hns1.02
Hydrophobic ICL2 P 173 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 173 34x50 G.H5 I 344 G.H5.16
Hydrophobic ICL2 P 173 34x50 G.H5 K 345 G.H5.17
Polar ICL3 E 291 - G.H5 N 346 G.H5.18
Van-der-waals ICL3 E 291 - G.H5 N 346 G.H5.18
Hydrophobic ICL2 L 179 34x56 G.hns1 R 32 G.hns1.03
Hydrophobic ICL1 L 94 - G.h2s4 E 216 G.h2s4.02
Van-der-waals ICL1 L 94 - G.h2s4 E 216 G.h2s4.02
Ionic TM6 R 294 6x26 G.h4s6 E 318 G.h4s6.12
Polar TM6 R 294 6x26 G.h4s6 E 318 G.h4s6.12
Hydrophobic TM6 G 301 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 G 301 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM2 V 101 2x39 G.H5 D 350 G.H5.22
Van-der-waals TM2 V 101 2x39 G.H5 D 350 G.H5.22
Polar ICL4 S 368 - G.H5 G 352 G.H5.24
Hydrophobic ICL4 S 368 - G.H5 G 352 G.H5.24
Van-der-waals ICL4 S 368 - G.H5 G 352 G.H5.24
Hydrophobic TM3 A 169 3x53 G.H5 L 348 G.H5.20
Hydrophobic ICL3 P 292 - G.H5 N 346 G.H5.18
Hydrophobic TM2 L 105 2x43 G.H5 G 352 G.H5.24
Polar ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 M 180 34x57 G.hns1 R 32 G.hns1.03
Hydrophobic TM3 I 170 3x54 G.H5 F 354 G.H5.26
Hydrophobic TM6 L 298 6x30 G.H5 F 354 G.H5.26
Hydrophobic TM4 S 181 4x38 G.HN E 28 G.HN.52
Hydrophobic TM6 L 305 6x37 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 305 6x37 G.H5 L 353 G.H5.25
Hydrophobic TM3 R 166 3x50 G.H5 L 353 G.H5.25
Van-der-waals TM3 R 166 3x50 G.H5 L 353 G.H5.25
Ionic TM3 R 166 3x50 G.H5 D 350 G.H5.22
Polar TM3 R 166 3x50 G.H5 D 350 G.H5.22
Hydrophobic TM3 R 166 3x50 G.H5 D 350 G.H5.22
Van-der-waals TM3 R 166 3x50 G.H5 D 350 G.H5.22
Hydrophobic TM5 L 263 5x65 G.H5 F 354 G.H5.26
Polar ICL2 K 177 34x54 G.hns1 A 31 G.hns1.02
Van-der-waals ICL2 K 177 34x54 G.hns1 A 31 G.hns1.02
Polar ICL1 Q 98 - G.hns1 A 31 G.hns1.02
Hydrophobic ICL1 Q 98 - G.hns1 A 31 G.hns1.02
Van-der-waals ICL1 Q 98 - G.hns1 A 31 G.hns1.02
Hydrophobic TM3 I 170 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 F 174 34x51 G.s2s3 K 192 G.s2s3.01
Hydrophobic ICL2 F 174 34x51 G.S3 L 194 G.S3.01
Hydrophobic ICL2 M 180 34x57 G.hns1 A 31 G.hns1.02
Polar ICL3 E 291 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 E 291 - G.h4s6 E 318 G.h4s6.12
Hydrophobic TM6 A 297 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM5 M 266 5x68 G.H5 K 345 G.H5.17
Hydrophobic ICL2 K 177 34x54 G.S3 L 194 G.S3.01
Van-der-waals ICL2 K 177 34x54 G.S3 L 194 G.S3.01
Polar ICL2 K 177 34x54 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 K 177 34x54 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 K 177 34x54 G.hns1 R 32 G.hns1.03
Polar TM4 S 183 4x40 G.HN E 28 G.HN.52
Hydrophobic TM4 S 183 4x40 G.HN E 28 G.HN.52
Hydrophobic TM5 M 266 5x68 G.H5 L 348 G.H5.20