Ggust - GPR4 complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gi/o

DATE

2023-09-15

3D VIEW

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Tertiary structure confidence

Chain pLDDT score avg
GPR4 77.4
Gαt3 87.8
Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.7
iPTM Score: 0.7
PAE mean: 13.6


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar ICL3 S 213 - G.H5 K 345 G.H5.17
Hydrophobic ICL3 S 213 - G.H5 K 345 G.H5.17
Van-der-waals ICL3 S 213 - G.H5 K 345 G.H5.17
Polar TM6 K 221 6x32 G.H5 F 354 G.H5.26
Hydrophobic TM6 K 221 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 K 221 6x32 G.H5 F 354 G.H5.26
Hydrophobic ICL3 V 212 - G.S6 Y 320 G.S6.02
Hydrophobic TM6 L 225 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 225 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 E 215 6x26 G.h4s6 E 315 G.h4s6.09
Van-der-waals TM6 E 215 6x26 G.h4s6 E 315 G.h4s6.09
Hydrophobic ICL2 P 122 34x50 G.H5 T 340 G.H5.12
Hydrophobic TM2 L 52 2x39 G.H5 C 351 G.H5.23
Van-der-waals TM2 L 52 2x39 G.H5 C 351 G.H5.23
Hydrophobic TM3 V 119 3x54 G.H5 I 344 G.H5.16
Van-der-waals TM3 V 119 3x54 G.H5 I 344 G.H5.16
Polar TM3 R 115 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 115 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 115 3x50 G.H5 C 351 G.H5.23
Hydrophobic ICL2 L 123 34x51 G.S3 L 194 G.S3.01
Van-der-waals ICL2 L 123 34x51 G.S3 L 194 G.S3.01
Hydrophobic ICL2 P 122 34x50 G.H5 I 343 G.H5.15
Van-der-waals ICL2 P 122 34x50 G.H5 I 343 G.H5.15
Hydrophobic ICL3 V 212 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 V 212 - G.h4s6 E 318 G.h4s6.12
Polar ICL3 S 213 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 S 213 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 S 213 - G.H5 D 341 G.H5.13
Hydrophobic TM3 R 115 3x50 G.H5 L 353 G.H5.25
Van-der-waals TM3 R 115 3x50 G.H5 L 353 G.H5.25
Hydrophobic TM6 L 229 6x40 G.H5 L 353 G.H5.25
Hydrophobic ICL2 R 127 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 R 127 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 L 123 34x51 G.H5 I 343 G.H5.15
Hydrophobic ICL2 P 122 34x50 G.H5 I 344 G.H5.16
Hydrophobic TM6 I 222 6x33 G.H5 L 348 G.H5.20
Van-der-waals ICL2 P 122 34x50 G.H5 N 347 G.H5.19
Hydrophobic TM5 V 208 5x65 G.H5 L 348 G.H5.20
Ionic ICL1 R 49 - G.HN E 20 G.HN.44
Polar ICL1 R 49 - G.HN E 20 G.HN.44
Polar ICL3 S 211 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 S 211 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 S 211 - G.H5 D 341 G.H5.13
Polar ICL3 V 212 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 V 212 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 V 212 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 S 213 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 S 213 - G.h4s6 E 318 G.h4s6.12
Hydrophobic H8 N 290 8x47 G.H5 G 352 G.H5.24
Van-der-waals H8 N 290 8x47 G.H5 G 352 G.H5.24
Polar TM3 R 115 3x50 G.H5 G 352 G.H5.24
Ionic TM4 R 130 4x38 G.HN E 28 G.HN.52
Polar TM4 R 130 4x38 G.HN E 28 G.HN.52
Hydrophobic TM4 R 130 4x38 G.HN E 28 G.HN.52
Van-der-waals TM4 R 130 4x38 G.HN E 28 G.HN.52
Hydrophobic TM3 A 118 3x53 G.H5 C 351 G.H5.23
Van-der-waals TM3 A 118 3x53 G.H5 C 351 G.H5.23
Polar ICL3 S 213 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 S 213 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 S 213 - G.S6 Y 320 G.S6.02
Hydrophobic TM6 I 222 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 I 222 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 119 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 V 119 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 L 123 34x51 G.H5 F 336 G.H5.08
Van-der-waals ICL2 L 123 34x51 G.H5 F 336 G.H5.08
Hydrophobic TM6 L 225 6x36 G.H5 G 352 G.H5.24
Van-der-waals TM6 L 225 6x36 G.H5 G 352 G.H5.24
Hydrophobic C-term M 357 - G.H2 R 205 G.H2.01
Hydrophobic TM6 E 218 6x29 G.H5 F 354 G.H5.26
Van-der-waals TM6 E 218 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM5 I 204 5x61 G.H5 L 353 G.H5.25
Hydrophobic TM6 I 222 6x33 G.H5 F 354 G.H5.26
Polar ICL2 L 123 34x51 G.hns1 R 32 G.hns1.03
Polar TM3 A 118 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 A 118 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 A 118 3x53 G.H5 N 347 G.H5.19
Polar TM2 N 50 2x37 G.H5 D 350 G.H5.22
Polar TM4 K 132 4x40 G.HN Q 24 G.HN.48
Van-der-waals TM4 K 132 4x40 G.HN Q 24 G.HN.48
Hydrophobic TM5 Y 201 5x58 G.H5 L 353 G.H5.25
Ionic TM6 E 218 6x29 G.H5 K 345 G.H5.17
Polar TM6 E 218 6x29 G.H5 K 345 G.H5.17
Hydrophobic TM6 E 218 6x29 G.H5 K 345 G.H5.17
Van-der-waals TM6 E 218 6x29 G.H5 K 345 G.H5.17