M2 receptor - Go

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

A

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

RESOLUTION

3.3 Å

REFERENCE

PUBLICATION DATE

Jan. 25, 2023

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

LIGANDS

ENDOGENOUS LIGANDS

acetylcholine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

, , , GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type
GPCR
Segment

AA

Number
Generic
number

segment

AA

Number
Generic
number
Polar H8 N 444 8x47 G.H5 G 350 G.H5.22
Polar ICL2 P 128 - G.H5 N 347 G.H5.19
Van-der-waals ICL2 P 128 - G.H5 N 347 G.H5.19
Aromatic TM6 K 384 6x32 G.H5 Y 354 G.H5.26
Hydrophobic TM6 K 384 6x32 G.H5 Y 354 G.H5.26
Hydrophobic TM6 I 389 6x37 G.H5 L 353 G.H5.25
Hydrophobic TM6 V 385 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM6 V 385 6x33 G.H5 L 348 G.H5.20
Van-der-waals TM6 V 385 6x33 G.H5 L 348 G.H5.20
Hydrophobic ICL4 C 443 - G.H5 G 352 G.H5.24
Van-der-waals ICL4 C 443 - G.H5 G 352 G.H5.24
Hydrophobic ICL3 I 217 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 I 217 - G.H5 D 341 G.H5.13
Polar ICL3 S 215 - G.H5 D 341 G.H5.13
Polar TM3 R 121 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 121 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 121 3x50 G.H5 C 351 G.H5.23
Hydrophobic H8 N 444 8x47 G.H5 G 352 G.H5.24
Ionic TM6 R 381 6x29 G.H5 D 341 G.H5.13
Polar TM6 R 381 6x29 G.H5 D 341 G.H5.13
Hydrophobic ICL2 P 128 - G.H5 I 343 G.H5.15
Hydrophobic TM5 S 213 5x65 G.H5 L 348 G.H5.20
Hydrophobic TM3 C 124 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM6 R 381 6x29 G.H5 Y 354 G.H5.26
Van-der-waals TM6 R 381 6x29 G.H5 Y 354 G.H5.26
Hydrophobic TM6 R 381 6x29 G.H5 A 345 G.H5.17
Van-der-waals TM6 R 381 6x29 G.H5 A 345 G.H5.17
Polar TM3 C 124 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL3 I 217 - G.H5 D 337 G.H5.09
Polar TM6 T 388 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 T 388 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 125 3x54 G.H5 L 348 G.H5.20
Hydrophobic TM6 R 381 6x29 G.H5 L 348 G.H5.20
Hydrophobic TM5 I 209 5x61 G.H5 L 353 G.H5.25
Van-der-waals TM5 I 209 5x61 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 128 - G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 128 - G.H5 I 344 G.H5.16