κ receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8F7W

RESOLUTION

3.2 Å

REFERENCE

10.1016/J.CELL.2022.12.026

PUBLICATION DATE

Dec. 14, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

59 interactions (Click to see)

LIGANDS

dynorphin A-(1-8) - Agonist pubchem gtoplig chembl_ligand pubchem pubchem

ENDOGENOUS LIGANDS

dynorphin A-(1-8), β-neoendorphin, big dynorphin, [Met]enkephalin, dynorphin A, [Leu]enkephalin, β-endorphin, dynorphin A-(1-13), dynorphin B, α-neoendorphin



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar ICL2 R 170 34x57 G.H5 C 351 G.H5.23
Hydrophobic ICL2 R 170 34x57 G.H5 C 351 G.H5.23
Van-der-waals ICL2 R 170 34x57 G.H5 C 351 G.H5.23
Polar TM6 K 265 6x26 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 K 265 6x26 G.h4s6 D 315 G.h4s6.09
Hydrophobic TM3 R 156 3x50 G.H5 C 351 G.H5.23
Polar H8 E 335 8x48 G.H5 G 352 G.H5.24
Van-der-waals H8 E 335 8x48 G.H5 G 352 G.H5.24
Hydrophobic ICL2 L 167 34x54 G.S3 L 194 G.S3.01
Hydrophobic TM6 K 265 6x26 G.H5 F 354 G.H5.26
Van-der-waals TM6 K 265 6x26 G.H5 F 354 G.H5.26
Hydrophobic ICL3 L 258 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 L 258 - G.S6 Y 320 G.S6.02
Ionic ICL2 D 168 34x55 G.hns1 R 32 G.hns1.03
Polar ICL2 D 168 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 D 168 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 D 168 34x55 G.hns1 R 32 G.hns1.03
Aromatic TM6 R 271 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 R 271 6x32 G.H5 F 354 G.H5.26
Polar ICL3 R 257 - G.S6 I 319 G.S6.01
Hydrophobic H8 N 336 8x49 G.H5 G 352 G.H5.24
Hydrophobic ICL3 L 258 - G.H5 D 341 G.H5.13
Hydrophobic TM5 L 253 5x65 G.H5 L 348 G.H5.20
Hydrophobic ICL2 A 166 34x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 V 164 34x51 G.H5 F 336 G.H5.08
Ionic ICL2 R 170 34x57 G.H5 D 350 G.H5.22
Hydrophobic ICL2 R 170 34x57 G.H5 D 350 G.H5.22
Van-der-waals ICL2 R 170 34x57 G.H5 D 350 G.H5.22
Hydrophobic TM6 I 272 6x33 G.H5 F 354 G.H5.26
Hydrophobic ICL2 P 163 34x50 G.H5 T 340 G.H5.12
Van-der-waals ICL2 P 163 34x50 G.H5 T 340 G.H5.12
Hydrophobic TM3 V 160 3x54 G.H5 L 348 G.H5.20
Hydrophobic TM5 M 249 5x61 G.H5 L 353 G.H5.25
Hydrophobic ICL3 R 257 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 R 257 - G.S6 Y 320 G.S6.02
Hydrophobic ICL2 P 163 34x50 G.H5 I 343 G.H5.15
Hydrophobic ICL3 L 258 - G.H5 K 345 G.H5.17
Ionic TM5 R 252 5x64 G.H5 D 341 G.H5.13
Hydrophobic TM3 A 159 3x53 G.H5 C 351 G.H5.23
Ionic ICL3 R 257 - G.h4s6 E 318 G.h4s6.12
Polar ICL3 R 257 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 R 257 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 R 257 - G.h4s6 E 318 G.h4s6.12
Hydrophobic TM6 L 275 6x36 G.H5 L 353 G.H5.25
Hydrophobic H8 E 335 8x48 G.H5 F 354 G.H5.26
Van-der-waals H8 E 335 8x48 G.H5 F 354 G.H5.26
Polar TM3 A 159 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 A 159 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 L 167 34x54 G.hns1 R 32 G.hns1.03
Hydrophobic TM6 N 268 6x29 G.H5 F 354 G.H5.26
Polar TM2 T 94 2x39 G.H5 C 351 G.H5.23
Hydrophobic ICL2 P 163 34x50 G.H5 I 344 G.H5.16
Hydrophobic TM6 I 272 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 I 272 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM5 R 252 5x64 G.H5 I 344 G.H5.16
Van-der-waals TM5 R 252 5x64 G.H5 I 344 G.H5.16
Hydrophobic ICL3 L 258 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 L 258 - G.h4s6 E 318 G.h4s6.12