RXFP3 - Gi2

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

C

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9KFJ

RESOLUTION

3.1 Å

REFERENCE

10.1038/S42003-025-08220-7

PUBLICATION DATE

Aug. 13, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

32 interactions (Click to see)

LIGANDS

CHEMBL4522365 - Agonist chembl_ligand pubchem

PHYSIOLOGICAL LIGANDS

INSL5 (Human), relaxin-3 (B chain) (Human), relaxin-3 (Human), relaxin (Human)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Aromatic TM6 R 320 6x29 G.H5 F 355 G.H5.26
Hydrophobic TM6 R 320 6x29 G.H5 F 355 G.H5.26
Van-der-waals TM6 R 320 6x29 G.H5 F 355 G.H5.26
Hydrophobic TM6 V 328 6x37 G.H5 L 354 G.H5.25
Ionic ICL2 R 192 - G.s2s3 D 194 G.s2s3.02
Polar ICL2 R 192 - G.s2s3 D 194 G.s2s3.02
Hydrophobic ICL2 R 192 - G.s2s3 D 194 G.s2s3.02
Van-der-waals ICL2 R 192 - G.s2s3 D 194 G.s2s3.02
Ionic ICL3 R 312 - G.h4s6 E 319 G.h4s6.12
Polar ICL3 R 312 - G.h4s6 E 319 G.h4s6.12
Hydrophobic ICL3 R 312 - G.h4s6 E 319 G.h4s6.12
Van-der-waals ICL3 R 312 - G.h4s6 E 319 G.h4s6.12
Polar ICL3 R 312 - G.S6 I 320 G.S6.01
Hydrophobic TM6 R 320 6x29 G.H5 L 349 G.H5.20
Ionic TM6 R 320 6x29 G.H5 D 342 G.H5.13
Polar TM6 R 320 6x29 G.H5 D 342 G.H5.13
Hydrophobic TM6 R 320 6x29 G.H5 D 342 G.H5.13
Van-der-waals TM6 R 320 6x29 G.H5 D 342 G.H5.13
Hydrophobic TM6 A 316 6x25 G.h4s6 E 319 G.h4s6.12
Van-der-waals TM6 A 316 6x25 G.h4s6 E 319 G.h4s6.12
Polar TM6 S 327 6x36 G.H5 L 354 G.H5.25
Hydrophobic TM6 S 327 6x36 G.H5 L 354 G.H5.25
Van-der-waals TM6 S 327 6x36 G.H5 L 354 G.H5.25
Hydrophobic TM6 V 324 6x33 G.H5 L 349 G.H5.20
Hydrophobic TM2 I 117 2x39 G.H5 C 352 G.H5.23
Van-der-waals TM2 I 117 2x39 G.H5 C 352 G.H5.23
Van-der-waals H8 R 394 8x48 G.H5 K 350 G.H5.21
Hydrophobic TM3 V 184 3x54 G.H5 L 349 G.H5.20
Van-der-waals TM3 V 184 3x54 G.H5 L 349 G.H5.20
Hydrophobic ICL2 H 191 - G.S3 L 195 G.S3.01
Van-der-waals ICL2 H 191 - G.S3 L 195 G.S3.01
Polar TM3 S 183 3x53 G.H5 N 348 G.H5.19
Hydrophobic TM5 L 290 5x61 G.H5 L 354 G.H5.25
Van-der-waals TM5 L 290 5x61 G.H5 L 354 G.H5.25
Hydrophobic H8 R 393 8x47 G.H5 G 353 G.H5.24
Hydrophobic TM6 V 324 6x33 G.H5 F 355 G.H5.26
Hydrophobic TM3 R 180 3x50 G.H5 L 354 G.H5.25
Van-der-waals TM3 R 180 3x50 G.H5 L 354 G.H5.25
Hydrophobic ICL2 A 187 - G.H5 I 344 G.H5.15
Hydrophobic ICL2 H 191 - G.H5 I 344 G.H5.15
Van-der-waals ICL2 H 191 - G.H5 I 344 G.H5.15
Polar ICL2 H 191 - G.hns1 A 31 G.hns1.02
Hydrophobic ICL2 A 187 - G.H5 N 348 G.H5.19
Van-der-waals ICL2 A 187 - G.H5 N 348 G.H5.19
Hydrophobic ICL2 L 188 - G.H5 I 344 G.H5.15
Hydrophobic ICL2 L 188 - G.H5 T 341 G.H5.12
Polar ICL2 R 192 - G.s2s3 K 193 G.s2s3.01
Polar ICL3 R 312 - G.S6 T 322 G.S6.03
Van-der-waals ICL3 R 312 - G.S6 T 322 G.S6.03
Ionic TM6 R 319 6x28 G.h4s6 D 316 G.h4s6.09
Hydrophobic TM6 R 319 6x28 G.h4s6 D 316 G.h4s6.09
Hydrophobic ICL2 S 190 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 S 190 - G.hns1 R 32 G.hns1.03
Hydrophobic TM5 R 297 5x68 G.H5 I 345 G.H5.16
Van-der-waals TM5 R 297 5x68 G.H5 I 345 G.H5.16
Hydrophobic TM6 R 320 6x29 G.H5 K 346 G.H5.17
Polar H8 R 394 8x48 G.H5 F 355 G.H5.26
Aromatic H8 R 394 8x48 G.H5 F 355 G.H5.26
Hydrophobic H8 R 394 8x48 G.H5 F 355 G.H5.26
Van-der-waals H8 R 394 8x48 G.H5 F 355 G.H5.26
Polar H8 R 393 8x47 G.H5 D 351 G.H5.22
Van-der-waals H8 R 393 8x47 G.H5 D 351 G.H5.22
Hydrophobic TM6 K 323 6x32 G.H5 F 355 G.H5.26
Van-der-waals TM6 K 323 6x32 G.H5 F 355 G.H5.26
Polar ICL2 R 192 - G.hns1 R 32 G.hns1.03
Hydrophobic TM3 V 184 3x54 G.H5 C 352 G.H5.23
Hydrophobic ICL2 H 191 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 H 191 - G.hns1 R 32 G.hns1.03
Polar TM3 R 180 3x50 G.H5 C 352 G.H5.23
Hydrophobic TM3 R 180 3x50 G.H5 C 352 G.H5.23
Van-der-waals TM3 R 180 3x50 G.H5 C 352 G.H5.23
Hydrophobic TM3 S 183 3x53 G.H5 C 352 G.H5.23
Van-der-waals TM3 S 183 3x53 G.H5 C 352 G.H5.23