M4 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Mus musculus

PDB CODE

8FX5

RESOLUTION

2.5 Å

REFERENCE

10.1038/S41467-023-41199-5

PUBLICATION DATE

Sept. 13, 2023

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

45 interactions (Click to see)

LIGANDS

xanomeline - Agonist pubchem gtoplig chembl_ligand drug_central drugbank

ENDOGENOUS LIGANDS

acetylcholine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 P 141 - G.H5 N 347 G.H5.19
Ionic TM6 R 394 6x29 G.H5 D 341 G.H5.13
Polar TM6 R 394 6x29 G.H5 D 341 G.H5.13
Hydrophobic TM6 R 394 6x29 G.H5 D 341 G.H5.13
Van-der-waals TM6 R 394 6x29 G.H5 D 341 G.H5.13
Hydrophobic TM6 V 398 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 V 398 6x33 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 137 - G.H5 T 340 G.H5.12
Polar ICL2 P 137 - G.H5 N 347 G.H5.19
Van-der-waals ICL2 P 137 - G.H5 N 347 G.H5.19
Hydrophobic TM3 V 134 3x54 G.H5 L 348 G.H5.20
Polar ICL3 R 225 - G.H5 D 341 G.H5.13
Hydrophobic TM5 Y 215 5x58 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 137 - G.H5 I 343 G.H5.15
Van-der-waals ICL2 P 137 - G.H5 I 343 G.H5.15
Polar TM6 T 401 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 T 401 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 R 394 6x29 G.H5 K 345 G.H5.17
Van-der-waals TM6 R 394 6x29 G.H5 K 345 G.H5.17
Van-der-waals ICL4 C 456 - G.H5 G 352 G.H5.24
Hydrophobic ICL3 S 222 - G.H5 L 348 G.H5.20
Hydrophobic ICL3 V 226 - G.S6 Y 320 G.S6.02
Polar TM3 C 133 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 C 133 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 C 133 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM2 N 67 2x39 G.H5 D 350 G.H5.22
Hydrophobic TM3 R 130 3x50 G.H5 L 353 G.H5.25
Van-der-waals TM3 R 130 3x50 G.H5 L 353 G.H5.25
Polar TM3 R 130 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 130 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 130 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM6 V 398 6x33 G.H5 F 354 G.H5.26
Ionic TM6 R 394 6x29 G.h4s6 E 318 G.h4s6.12
Polar TM6 R 394 6x29 G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL2 L 138 - G.H5 F 336 G.H5.08
Hydrophobic ICL2 L 138 - G.S3 L 194 G.S3.01
Hydrophobic ICL2 A 142 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 P 137 - G.H5 I 344 G.H5.16
Polar TM3 C 133 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM3 C 133 3x53 G.H5 C 351 G.H5.23
Van-der-waals TM3 C 133 3x53 G.H5 C 351 G.H5.23
Polar TM2 N 67 2x39 G.H5 C 351 G.H5.23
Hydrophobic TM2 N 67 2x39 G.H5 C 351 G.H5.23
Van-der-waals TM2 N 67 2x39 G.H5 C 351 G.H5.23
Hydrophobic ICL2 L 138 - G.H5 I 343 G.H5.15
Hydrophobic TM5 I 218 5x61 G.H5 L 353 G.H5.25
Hydrophobic TM6 V 398 6x33 G.H5 L 348 G.H5.20
Hydrophobic TM6 I 402 6x37 G.H5 L 353 G.H5.25
Polar ICL3 S 224 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 S 224 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 S 224 - G.H5 D 341 G.H5.13
Aromatic TM6 R 394 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM6 R 394 6x29 G.H5 F 354 G.H5.26
Van-der-waals TM6 R 394 6x29 G.H5 F 354 G.H5.26
Aromatic TM6 K 397 6x32 G.H5 F 354 G.H5.26
Hydrophobic TM6 K 397 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 K 397 6x32 G.H5 F 354 G.H5.26
Hydrophobic TM6 R 394 6x29 G.H5 L 348 G.H5.20