Refined structure (7WU9) of Gi1 - EP3 receptor

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gi/o

DATE

2024-02-01

3D VIEW

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Structure
GPCR

Model
AlphaFold2-Multimer

REFINED G PROTEIN RESIDUES

Residue backbones - 135 from structure (38.1%), 219 from model (61.9%)
Residue sidechains - 117 from structure (33.1%), 237 from model (66.9%)

REFINED GPCR RESIDUES

Residue backbones - 270 from structure (71.2%), 109 from model (28.8%)
Residue sidechains - 173 from structure (45.6%), 206 from model (54.4%)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:
R03G.hns1Q12E16G.H4D09E12G.h4s6Y02G.S6T12D13D22C23G24G.H5GproteinS-Y34x53K2x36H34x56R8x58S6x22R3x50Q6x36R5x68Q6x25R-S-K5x72S2x38Q-GPCRInteractions:AromaticHydrophobicIonicPolarVan der waalsG Protein segments:G.hns1G.H4G.h4s6G.S6G.H5GPCR segments:ICL1TM2ICL2TM3ICL3TM5TM6H8

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

ACDEFGHIKLMNPQRSTVWY-_+van-der-waalshydrophobicionicaromaticpolar
null2x362x382x393x493x503x533x5434x5034x5134x5334x5534x565x655x685x726x226x256x266x296x336x368x58G.H5.26G.H5.25G.H5.24G.H5.23G.H5.22G.H5.21G.H5.20G.H5.19G.H5.17G.H5.16G.H5.15G.H5.13G.H5.12G.S6.02G.h4s6.12G.h4s6.09G.h4s6.08G.H4.16G.H4.12G.S3.01G.hns1.03G.hns1.02G.HN.52Res. Pos.EP3 (7WU9_REFINED)- (7WU9_REFINED)-----------------------KSFERAIPHYSHLRKSQWITQRKSFPHYSHERAILRKSQWITQRSKDCGIINLEARLLTDYEKDKFQE

, , , GPCR
Interaction cut-off: strict (stick), loose (line)

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