M4 receptor (acm4_human)
FAMILY
Class A (Rhodopsin) Aminergic receptors Acetylcholine receptors (muscarinic) M4 receptor
GENE
CHRM4
ORGANISM
Human (Homo sapiens)
ALT. NAMES
Muscarinic acetylcholine receptor M4
SOURCE
SWISSPROT
SEQUENCE
N-term | |||||||||
M
|
A
|
N
|
F
|
T
|
P
|
V
|
N
|
G
|
S
|
10 |
S
|
G
|
N
|
Q
|
S
|
V
|
R
|
L
|
V
|
T
|
20 |
TM1 | |||||||||
S
|
S
|
S
|
H
|
N
|
R
|
Y
|
E
|
T
|
V
|
30 |
E
|
M
|
V
|
F
|
I
|
A
|
T
|
V
|
T
|
G
|
40 |
S
|
L
|
S
|
L
|
V
|
T
|
V
|
V
|
G
|
N
|
50 |
I
|
L
|
V
|
M
|
L
|
S
|
I
|
K
|
V
|
N
|
60 |
ICL1 | TM2 | ||||||||
R
|
Q
|
L
|
Q
|
T
|
V
|
N
|
N
|
Y
|
F
|
70 |
L
|
F
|
S
|
L
|
A
|
C
|
A
|
D
|
L
|
I
|
80 |
I
|
G
|
A
|
F
|
S
|
M
|
N
|
L
|
Y
|
T
|
90 |
ECL1 | |||||||||
V
|
Y
|
I
|
I
|
K
|
G
|
Y
|
W
|
P
|
L
|
100 |
TM3 | |||||||||
G
|
A
|
V
|
V
|
C
|
D
|
L
|
W
|
L
|
A
|
110 |
L
|
D
|
Y
|
V
|
V
|
S
|
N
|
A
|
S
|
V
|
120 |
M
|
N
|
L
|
L
|
I
|
I
|
S
|
F
|
D
|
R
|
130 |
ICL2 | |||||||||
Y
|
F
|
C
|
V
|
T
|
K
|
P
|
L
|
T
|
Y
|
140 |
TM4 | |||||||||
P
|
A
|
R
|
R
|
T
|
T
|
K
|
M
|
A
|
G
|
150 |
L
|
M
|
I
|
A
|
A
|
A
|
W
|
V
|
L
|
S
|
160 |
F
|
V
|
L
|
W
|
A
|
P
|
A
|
I
|
L
|
F
|
170 |
ECL2 | |||||||||
W
|
Q
|
F
|
V
|
V
|
G
|
K
|
R
|
T
|
V
|
180 |
P
|
D
|
N
|
Q
|
C
|
F
|
I
|
Q
|
F
|
L
|
190 |
TM5 | |||||||||
S
|
N
|
P
|
A
|
V
|
T
|
F
|
G
|
T
|
A
|
200 |
I
|
A
|
A
|
F
|
Y
|
L
|
P
|
V
|
V
|
I
|
210 |
M
|
T
|
V
|
L
|
Y
|
I
|
H
|
I
|
S
|
L
|
220 |
A
|
S
|
R
|
S
|
R
|
V
|
H
|
K
|
H
|
R
|
230 |
ICL3 | |||||||||
P
|
E
|
G
|
P
|
K
|
E
|
K
|
K
|
A
|
K
|
240 |
T
|
L
|
A
|
F
|
L
|
K
|
S
|
P
|
L
|
M
|
250 |
K
|
Q
|
S
|
V
|
K
|
K
|
P
|
P
|
P
|
G
|
260 |
E
|
A
|
A
|
R
|
E
|
E
|
L
|
R
|
N
|
G
|
270 |
K
|
L
|
E
|
E
|
A
|
P
|
P
|
P
|
A
|
L
|
280 |
P
|
P
|
P
|
P
|
R
|
P
|
V
|
A
|
D
|
K
|
290 |
D
|
T
|
S
|
N
|
E
|
S
|
S
|
S
|
G
|
S
|
300 |
A
|
T
|
Q
|
N
|
T
|
K
|
E
|
R
|
P
|
A
|
310 |
T
|
E
|
L
|
S
|
T
|
T
|
E
|
A
|
T
|
T
|
320 |
P
|
A
|
M
|
P
|
A
|
P
|
P
|
L
|
Q
|
P
|
330 |
R
|
A
|
L
|
N
|
P
|
A
|
S
|
R
|
W
|
S
|
340 |
K
|
I
|
Q
|
I
|
V
|
T
|
K
|
Q
|
T
|
G
|
350 |
N
|
E
|
C
|
V
|
T
|
A
|
I
|
E
|
I
|
V
|
360 |
P
|
A
|
T
|
P
|
A
|
G
|
M
|
R
|
P
|
A
|
370 |
A
|
N
|
V
|
A
|
R
|
K
|
F
|
A
|
S
|
I
|
380 |
A
|
R
|
N
|
Q
|
V
|
R
|
K
|
K
|
R
|
Q
|
390 |
TM6 | |||||||||
M
|
A
|
A
|
R
|
E
|
R
|
K
|
V
|
T
|
R
|
400 |
T
|
I
|
F
|
A
|
I
|
L
|
L
|
A
|
F
|
I
|
410 |
L
|
T
|
W
|
T
|
P
|
Y
|
N
|
V
|
M
|
V
|
420 |
ECL3 | |||||||||
L
|
V
|
N
|
T
|
F
|
C
|
Q
|
S
|
C
|
I
|
430 |
TM7 | |||||||||
P
|
D
|
T
|
V
|
W
|
S
|
I
|
G
|
Y
|
W
|
440 |
L
|
C
|
Y
|
V
|
N
|
S
|
T
|
I
|
N
|
P
|
450 |
H8 | |||||||||
A
|
C
|
Y
|
A
|
L
|
C
|
N
|
A
|
T
|
F
|
460 |
K
|
K
|
T
|
F
|
R
|
H
|
L
|
L
|
L
|
C
|
470 |
C-term | ||||||||
Q
|
Y
|
R
|
N
|
I
|
G
|
T
|
A
|
R
|
DIAGRAMS
Pick color:
Invitro Mutant Data: Increased binding/potency: >5-fold, >10-fold; Reduced binding/potency: >5-fold, >10-fold; No/low effect (<5-fold); and N/A
Pick color:
Invitro Mutant Data: Increased binding/potency: >5-fold, >10-fold; Reduced binding/potency: >5-fold, >10-fold; No/low effect (<5-fold); and N/A
MUTATIONS
PHYSIOLOGICAL LIGANDS
acetylcholine