M2 receptor - Go

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

A

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

RESOLUTION

3.2 Å

REFERENCE

PUBLICATION DATE

Jan. 25, 2023

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

LIGANDS

ENDOGENOUS LIGANDS

acetylcholine



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

, , , GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type
GPCR
Segment

AA

Number
Generic
number

segment

AA

Number
Generic
number
Polar ICL3 R 216 - G.H5 D 337 G.H5.09
Hydrophobic ICL3 R 216 - G.H5 D 337 G.H5.09
Van-der-waals ICL3 R 216 - G.H5 D 337 G.H5.09
Polar ICL2 P 128 34x50 G.H5 N 347 G.H5.19
Hydrophobic ICL2 P 128 34x50 G.H5 N 347 G.H5.19
Van-der-waals ICL2 P 128 34x50 G.H5 N 347 G.H5.19
Hydrophobic ICL2 V 133 34x55 G.HN I 28 G.HN.52
Hydrophobic TM6 K 384 6x32 G.H5 Y 354 G.H5.26
Hydrophobic TM6 I 389 6x37 G.H5 L 353 G.H5.25
Van-der-waals TM6 I 389 6x37 G.H5 L 353 G.H5.25
Polar TM2 N 58 2x39 G.H5 C 351 G.H5.23
Van-der-waals TM2 N 58 2x39 G.H5 C 351 G.H5.23
Hydrophobic ICL2 T 130 34x52 G.hns1 K 32 G.hns1.03
Polar TM2 N 58 2x39 G.H5 G 350 G.H5.22
Polar ICL3 R 216 - G.H5 D 341 G.H5.13
Hydrophobic ICL3 R 216 - G.H5 D 341 G.H5.13
Hydrophobic TM6 V 385 6x33 G.H5 L 348 G.H5.20
Hydrophobic ICL3 I 217 - G.H5 A 338 G.H5.10
Hydrophobic ICL3 I 217 - G.H5 D 341 G.H5.13
Hydrophobic ICL4 C 443 - G.H5 G 352 G.H5.24
Van-der-waals ICL4 C 443 - G.H5 G 352 G.H5.24
Hydrophobic ICL2 L 129 34x51 G.hns1 K 32 G.hns1.03
Polar TM3 R 121 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 121 3x50 G.H5 C 351 G.H5.23
Polar ICL3 R 216 - G.H5 T 340 G.H5.12
Hydrophobic ICL3 R 216 - G.H5 T 340 G.H5.12
Van-der-waals ICL3 R 216 - G.H5 T 340 G.H5.12
Ionic TM6 R 381 6x29 G.H5 D 341 G.H5.13
Polar TM6 R 381 6x29 G.H5 D 341 G.H5.13
Hydrophobic TM6 R 381 6x29 G.H5 D 341 G.H5.13
Van-der-waals TM6 R 381 6x29 G.H5 D 341 G.H5.13
Hydrophobic ICL2 P 128 34x50 G.H5 I 343 G.H5.15
Van-der-waals ICL2 P 128 34x50 G.H5 I 343 G.H5.15
Hydrophobic ICL2 L 129 34x51 G.S3 L 195 G.S3.01
Van-der-waals ICL2 L 129 34x51 G.S3 L 195 G.S3.01
Hydrophobic TM5 S 213 5x65 G.H5 L 348 G.H5.20
Van-der-waals TM5 S 213 5x65 G.H5 L 348 G.H5.20
Hydrophobic TM3 C 124 3x53 G.H5 C 351 G.H5.23
Van-der-waals TM3 C 124 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM6 R 381 6x29 G.H5 Y 354 G.H5.26
Van-der-waals TM6 R 381 6x29 G.H5 Y 354 G.H5.26
Van-der-waals TM6 R 381 6x29 G.H5 A 345 G.H5.17
Hydrophobic ICL3 I 217 - G.S6 Y 320 G.S6.02
Polar TM3 C 124 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 C 124 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 C 124 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL3 I 217 - G.H5 D 337 G.H5.09
Van-der-waals ICL3 I 217 - G.H5 D 337 G.H5.09
Hydrophobic TM6 T 388 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 T 388 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 125 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 V 125 3x54 G.H5 L 348 G.H5.20
Hydrophobic TM6 R 381 6x29 G.H5 L 348 G.H5.20
Van-der-waals TM6 R 381 6x29 G.H5 L 348 G.H5.20
Hydrophobic ICL2 K 134 34x56 G.HN I 28 G.HN.52
Hydrophobic TM5 I 209 5x61 G.H5 L 353 G.H5.25
Van-der-waals TM5 I 209 5x61 G.H5 L 353 G.H5.25
Hydrophobic ICL2 V 133 34x55 G.hns1 A 31 G.hns1.02
Hydrophobic TM3 R 121 3x50 G.H5 L 353 G.H5.25
Hydrophobic ICL2 L 129 34x51 G.H5 I 343 G.H5.15
Van-der-waals ICL2 L 129 34x51 G.H5 I 343 G.H5.15