Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assayAgonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assayAgonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assayAgonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assayAgonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)Positive allosteric modulator activity at human dopamine D5 receptor expressed in CHO-K1 cells assessed as potentiation of dopamine-induced beta-arrestin2 recruitment by measuring dopamine EC50 at 50 uM incubated for 90 mins by PathHunter assay (Rvb = 81.7 nM)
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Displacement of [3H]SCH 23390 from human recombinant D5 receptor expressed in GH4 cells measured after 60 mins by scintillation counting methodDisplacement of [3H]SCH 23390 from human recombinant D5 receptor expressed in GH4 cells measured after 60 mins by scintillation counting method
Displacement of [3H]SCH23390 from human recombinant Dopamine D5 receptor expressed in GH4 cellsDisplacement of [3H]SCH23390 from human recombinant Dopamine D5 receptor expressed in GH4 cells
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human dopamine D5 receptor by radioligand displacement assayBinding affinity to human dopamine D5 receptor by radioligand displacement assay
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of [3H]SCH 23390 from human D5 dopamine receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human D5 dopamine receptor expressed in HEK293 cells
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of [3H]radioligand from human recombinant dopamine D5 receptor expressed in HEK293 cells by microplate scintillation countingDisplacement of [3H]radioligand from human recombinant dopamine D5 receptor expressed in HEK293 cells by microplate scintillation counting
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor by liquid scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor by liquid scintillation counting
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Radioligand Binding Assay: Membranes prepared as above were resuspended to 1 ug protein/ul (measured by Bradford assay using BSA as standard), and 50 ul were added to each well of a polypropylene 96-well plate containing (per well): 50 ul of buffer (20 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 100 mM N-methyl-D-gluconate, pH 7.4), 50 ul of 1.5 nM [3H]N-methylspiperone (final concentration 0.3 nM) and reference or D2 test ligand at various concentrations ranging from 50 uM to 50 uM (final concentrations ranging from 10 uM to 10 uM, triplicate determinations for each concentration of D2 test ligand). After a 1.5-hr incubation in the dark at room temperature, the reactions were harvested onto 0.3% PEI-soaked Filtermax GF/A filters (Wallac) and washed three times with ice-cold 50 mM Tris, pH 7.4 using a Perkin-Elmer Filtermate 96-well harvester. The filters were subsequently dried, placed on a hot plate (100° C.), and Melitilex-A (Wallac) scintillant was applied.Radioligand Binding Assay: Membranes prepared as above were resuspended to 1 ug protein/ul (measured by Bradford assay using BSA as standard), and 50 ul were added to each well of a polypropylene 96-well plate containing (per well): 50 ul of buffer (20 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 100 mM N-methyl-D-gluconate, pH 7.4), 50 ul of 1.5 nM [3H]N-methylspiperone (final concentration 0.3 nM) and reference or D2 test ligand at various concentrations ranging from 50 uM to 50 uM (final concentrations ranging from 10 uM to 10 uM, triplicate determinations for each concentration of D2 test ligand). After a 1.5-hr incubation in the dark at room temperature, the reactions were harvested onto 0.3% PEI-soaked Filtermax GF/A filters (Wallac) and washed three times with ice-cold 50 mM Tris, pH 7.4 using a Perkin-Elmer Filtermate 96-well harvester. The filters were subsequently dried, placed on a hot plate (100° C.), and Melitilex-A (Wallac) scintillant was applied.
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes after 90 mins by scintillation counting methodDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes after 90 mins by scintillation counting method
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cells
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranesDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranes
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor by liquid scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor by liquid scintillation counting
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Activity at human D5 dopamine receptor expressed in HEK293 cells by calcium fluorescence assayActivity at human D5 dopamine receptor expressed in HEK293 cells by calcium fluorescence assay
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH233930 from dopamine D5 receptor after 1.5 hrs by scintillation countingDisplacement of [3H]SCH233930 from dopamine D5 receptor after 1.5 hrs by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Antagonist activity at human Dopamine D5 receptor in HEK293 cells assessed as change in SKF 38393-induced intracellular calcium responseAntagonist activity at human Dopamine D5 receptor in HEK293 cells assessed as change in SKF 38393-induced intracellular calcium response
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Displacement of [3H]SCH23390 from human D5 receptor expressed in CH4Cl cells incubated for 60 mins by radio ligand binding assayDisplacement of [3H]SCH23390 from human D5 receptor expressed in CH4Cl cells incubated for 60 mins by radio ligand binding assay
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]radioligand from human recombinant dopamine D5 receptor expressed in HEK293 cells by microplate scintillation countingDisplacement of [3H]radioligand from human recombinant dopamine D5 receptor expressed in HEK293 cells by microplate scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from rat dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from rat dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranesDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Displacement of [3H]radioligand from human recombinant dopamine D5 receptor expressed in HEK293 cells by microplate scintillation countingDisplacement of [3H]radioligand from human recombinant dopamine D5 receptor expressed in HEK293 cells by microplate scintillation counting
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Activity at human D5 dopamine receptor expressed in HEK293 cells by calcium fluorescence assayActivity at human D5 dopamine receptor expressed in HEK293 cells by calcium fluorescence assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysis
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH-23390 from human cloned D5 dopamine receptor expressed in HEKT cells incubated for 90 mins by scintillation counting based radioligand binding assayDisplacement of [3H]SCH-23390 from human cloned D5 dopamine receptor expressed in HEKT cells incubated for 90 mins by scintillation counting based radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysis
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysis
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Compound was tested for the Binding affinity against Human cloned Dopamine receptor D5 by Radio ligand ([3H]SCH-23390) binding assayCompound was tested for the Binding affinity against Human cloned Dopamine receptor D5 by Radio ligand ([3H]SCH-23390) binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting method
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranesDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranesDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEK cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEK cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cells after 1 hrDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cells after 1 hr
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by functional calcium assay
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranesDisplacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting method
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranesDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranes
Displacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranesDisplacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranes
Displacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranesDisplacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Antagonist activity at human Dopamine D5 receptor in HEK293 cells assessed as change in SKF 38393-induced intracellular calcium responseAntagonist activity at human Dopamine D5 receptor in HEK293 cells assessed as change in SKF 38393-induced intracellular calcium response
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cells
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cells
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor by liquid scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor by liquid scintillation counting
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysis
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Binding affinity against human Dopamine receptor D4 versus hD2, hD3 receptorBinding affinity against human Dopamine receptor D4 versus hD2, hD3 receptor
Binding affinity against human Dopamine receptor D4 versus hD2, hD3 receptorBinding affinity against human Dopamine receptor D4 versus hD2, hD3 receptor
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Radioligand Binding Assay: Membranes prepared as above were resuspended to 1 ug protein/ul (measured by Bradford assay using BSA as standard), and 50 ul were added to each well of a polypropylene 96-well plate containing (per well): 50 ul of buffer (20 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 100 mM N-methyl-D-gluconate, pH 7.4), 50 ul of 1.5 nM [3H]N-methylspiperone (final concentration 0.3 nM) and reference or D2 test ligand at various concentrations ranging from 50 uM to 50 uM (final concentrations ranging from 10 uM to 10 uM, triplicate determinations for each concentration of D2 test ligand). After a 1.5-hr incubation in the dark at room temperature, the reactions were harvested onto 0.3% PEI-soaked Filtermax GF/A filters (Wallac) and washed three times with ice-cold 50 mM Tris, pH 7.4 using a Perkin-Elmer Filtermate 96-well harvester. The filters were subsequently dried, placed on a hot plate (100° C.), and Melitilex-A (Wallac) scintillant was applied.Radioligand Binding Assay: Membranes prepared as above were resuspended to 1 ug protein/ul (measured by Bradford assay using BSA as standard), and 50 ul were added to each well of a polypropylene 96-well plate containing (per well): 50 ul of buffer (20 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 100 mM N-methyl-D-gluconate, pH 7.4), 50 ul of 1.5 nM [3H]N-methylspiperone (final concentration 0.3 nM) and reference or D2 test ligand at various concentrations ranging from 50 uM to 50 uM (final concentrations ranging from 10 uM to 10 uM, triplicate determinations for each concentration of D2 test ligand). After a 1.5-hr incubation in the dark at room temperature, the reactions were harvested onto 0.3% PEI-soaked Filtermax GF/A filters (Wallac) and washed three times with ice-cold 50 mM Tris, pH 7.4 using a Perkin-Elmer Filtermate 96-well harvester. The filters were subsequently dried, placed on a hot plate (100° C.), and Melitilex-A (Wallac) scintillant was applied.
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assay
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation countingDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in CHO cells by scintillation counting
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranesDisplacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranes
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Radioligand Binding Assay: Membranes prepared as above were resuspended to 1 ug protein/ul (measured by Bradford assay using BSA as standard), and 50 ul were added to each well of a polypropylene 96-well plate containing (per well): 50 ul of buffer (20 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 100 mM N-methyl-D-gluconate, pH 7.4), 50 ul of 1.5 nM [3H]N-methylspiperone (final concentration 0.3 nM) and reference or D2 test ligand at various concentrations ranging from 50 uM to 50 uM (final concentrations ranging from 10 uM to 10 uM, triplicate determinations for each concentration of D2 test ligand). After a 1.5-hr incubation in the dark at room temperature, the reactions were harvested onto 0.3% PEI-soaked Filtermax GF/A filters (Wallac) and washed three times with ice-cold 50 mM Tris, pH 7.4 using a Perkin-Elmer Filtermate 96-well harvester. The filters were subsequently dried, placed on a hot plate (100° C.), and Melitilex-A (Wallac) scintillant was applied.Radioligand Binding Assay: Membranes prepared as above were resuspended to 1 ug protein/ul (measured by Bradford assay using BSA as standard), and 50 ul were added to each well of a polypropylene 96-well plate containing (per well): 50 ul of buffer (20 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 100 mM N-methyl-D-gluconate, pH 7.4), 50 ul of 1.5 nM [3H]N-methylspiperone (final concentration 0.3 nM) and reference or D2 test ligand at various concentrations ranging from 50 uM to 50 uM (final concentrations ranging from 10 uM to 10 uM, triplicate determinations for each concentration of D2 test ligand). After a 1.5-hr incubation in the dark at room temperature, the reactions were harvested onto 0.3% PEI-soaked Filtermax GF/A filters (Wallac) and washed three times with ice-cold 50 mM Tris, pH 7.4 using a Perkin-Elmer Filtermate 96-well harvester. The filters were subsequently dried, placed on a hot plate (100° C.), and Melitilex-A (Wallac) scintillant was applied.
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assay
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cells
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Inhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assayInhibition of D5 dopamine receptor in HEK 293 cells by intracellular calcium assay
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Binding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assayBinding affinity to human cloned D5 dopamine receptor incubated for 90 mins by radioligand binding assay
Binding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cellsBinding affinity to human cloned dopamine D5 receptor expressed in HEK 293 cells
Displacement of [3H]SCH 23390 from human D5 dopamine receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human D5 dopamine receptor expressed in HEK293 cells
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 1 hr by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranesDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cell membranes
Activity at human D5 dopamine receptor expressed in HEK293 cells by calcium fluorescence assayActivity at human D5 dopamine receptor expressed in HEK293 cells by calcium fluorescence assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibition of human cloned dopamine D5 receptor by competitive binding experimentInhibition of human cloned dopamine D5 receptor by competitive binding experiment
Inhibition of human cloned dopamine D5 receptor by competitive binding experimentInhibition of human cloned dopamine D5 receptor by competitive binding experiment
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cells by radioligand binding assay
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranesDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Compound was tested for the Binding affinity against Human cloned Dopamine receptor D5 by Radio ligand ([3H]SCH-23390) binding assayCompound was tested for the Binding affinity against Human cloned Dopamine receptor D5 by Radio ligand ([3H]SCH-23390) binding assay
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Displacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranesDisplacement of [3H]SCH23990 from human D5 receptor expressed in HEK293T cell membranes
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293Displacement of [3H]SCH 23390 from human Dopamine D5 receptor expressed in HEK293
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting method
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors.Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors.
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation countingDisplacement of radioligand from dopamine D5 receptor expressed in mouse LTK cells by scintillation counting
Inhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assayInhibition of binding to human D5 receptor expressed in HEK 293 cells by radioligand binding assay
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cells after 1 hrDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293 cells after 1 hr
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH-23390 from human cloned D5 dopamine receptor expressed in HEKT cells incubated for 90 mins by scintillation counting based radioligand binding assayDisplacement of [3H]SCH-23390 from human cloned D5 dopamine receptor expressed in HEKT cells incubated for 90 mins by scintillation counting based radioligand binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brainInhibition of [3H]-SCH- 23390 binding to Dopamine receptor D1-like in rat brain
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in human HEK293 cells
Displacement of [3H]SCH 23390 from human D5 dopamine receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human D5 dopamine receptor expressed in HEK293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cellsDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK cells
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-SCH23390 from recombinant human D5 receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
Displacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human D5R expressed in HEK293T cell membranes by radioligand binding assay
Displacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cellsDisplacement of [3H]SCH 23390 from human dopamine D5 receptor expressed in HEK293 cells
Displacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assayDisplacement of [3H]SCH23390 from human dopamine D5 receptor expressed in HEK293T cell membranes by radioligand binding assay
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Inhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranesInhibitory constant for [3H]SCH-23390 binding to Dopamine receptor D1-like of porcine striatal membranes
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
Displacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysisDisplacement of [3H]SCH23390 from human dopamine D5 receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis