GPCRdb

Ligand source activities (1 row/activity)

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Ligands					Receptor			Assay information							Chemical information
Sel. page	Common name	GPCRdb ID	#Vendors	Reference ligand	Fold selectivity (Potency)	# tested GPCRs (Potency)	Species	p-value (-log)	Type	Activity Relation	Activity Value	Assay Type	Assay Description	Source	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles	DOI
	155524115	176523	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	155524115	176523	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	155524115	176523	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	46235428	6572	0	None	263	3	Human	9.3	pEC50	=	9.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6572	0	None	263	3	Human	9.3	pEC50	=	9.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	58227401	176427	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	58227401	176427	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	51354300	58859	0	None	-	1	Human	9.0	pEC50	=	9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688946	58859	0	None	-	1	Human	9.0	pEC50	=	9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	155491015	176471	4	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	176471	4	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	176471	4	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	155513612	176296	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	58227401	176427	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	45257132	3656	10	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3656	10	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3656	10	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	155552061	176454	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	155517880	176643	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	45257132	3656	10	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3656	10	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3656	10	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	58227373	176484	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	136030655	56452	0	None	1	3	Mouse	8.0	pEC50	=	8	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56452	0	None	1	3	Mouse	8.0	pEC50	=	8	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136030655	56452	0	None	-1	3	Rat	8.0	pEC50	=	8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56452	0	None	-1	3	Rat	8.0	pEC50	=	8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25068753	188414	19	None	-1	2	Rat	8.0	pEC50	=	8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	188414	19	None	-1	2	Rat	8.0	pEC50	=	8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	10105117	104168	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL309819	104168	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	127051301	140175	0	None	4	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	140175	0	None	4	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	136141537	56451	3	None	1	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56451	3	None	1	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	4024	2002	20	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	9989505	2002	20	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL82298	2002	20	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	58227259	117116	0	None	-	1	Human	6.0	pEC50	=	6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	117116	0	None	-	1	Human	6.0	pEC50	=	6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1194	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	23786499	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	CHEMBL26116	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	24797598	61532	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771001	61532	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	11846299	139195	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	CHEMBL378788	139195	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	46228114	200153	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596551	200153	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25130576	187602	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187602	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130232	187539	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cn(C)cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494576	187539	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cn(C)cn3)nc(N)n2)CC1	10.1021/jm8005959
	25131229	187571	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	CHEMBL494724	187571	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	25130576	187602	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187602	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	11846635	78651	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	CHEMBL211242	78651	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	46228132	202122	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL609161	202122	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	25129178	187605	1	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL494910	187605	1	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	1267	3775	49	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	3035905	3775	49	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	CHEMBL260374	3775	49	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	44480708	5743	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(/NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1021/jm900526h
	CHEMBL1078632	5743	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(/NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1021/jm900526h
	53233804	61496	0	None	-	1	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770966	61496	0	None	-	1	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	1204	1919	119	None	-3	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1919	119	None	-3	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1919	119	None	-3	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1919	119	None	-3	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1919	119	None	-3	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1919	119	None	-3	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	127052293	140213	3	None	10	2	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	140213	3	None	10	2	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1247	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1375	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	774	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	CHEMBL90	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	DB05381	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	45257131	199885	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	199885	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	56593499	140217	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	6	3	4	3.2	N#C/N=C(/NCCSc1ccccc1)N[C@H]1CC[C@@H](c2c[nH]cn2)CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805392	140217	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	6	3	4	3.2	N#C/N=C(/NCCSc1ccccc1)N[C@H]1CC[C@@H](c2c[nH]cn2)CC1	10.1021/acs.jmedchem.6b00120
	127050776	140222	0	None	3	3	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	140222	0	None	3	3	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	127050431	140290	0	None	3	2	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	140290	0	None	3	2	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227165	117096	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393536	117096	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	25130578	187309	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	251	4	1	5	1.4	CN(C)CCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL493099	187309	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	251	4	1	5	1.4	CN(C)CCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	25129525	187559	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494648	187559	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	25128833	188378	38	None	-2	2	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	CHEMBL501882	188378	38	None	-2	2	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	25128831	172589	0	None	1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL449157	172589	0	None	1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	11043445	56449	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL1632410	56449	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	16061839	188394	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	284	3	2	6	1.6	CN1CCN(c2cc(Nc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL502104	188394	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	284	3	2	6	1.6	CN1CCN(c2cc(Nc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	127050431	140290	0	None	3	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	140290	0	None	3	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	54585780	61528	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770998	61528	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	1204	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	1247	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	1375	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	774	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	CHEMBL90	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	DB05381	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	25130914	187230	0	None	15	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL492677	187230	0	None	15	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	11957573	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	1250	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	3692	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	CHEMBL19439	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	11957573	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1250	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3692	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19439	1997	34	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1253	2045	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	132937	2045	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19010	2045	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10176414	4032	16	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1246	4032	16	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL281576	4032	16	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1267	3775	49	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	44482032	5700	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	262	7	3	3	1.4	CC(C)CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1078346	5700	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	262	7	3	3	1.4	CC(C)CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	25130581	187201	0	None	3	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL492489	187201	0	None	3	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	44482029	5604	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1cccnc1	10.1021/jm900526h
	CHEMBL1077737	5604	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1cccnc1	10.1021/jm900526h
	25131234	187638	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccccc3C#N)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495083	187638	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccccc3C#N)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187563	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187563	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187563	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187563	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	25130234	187379	0	None	-	1	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	2	6	-0.7	CN1CCN(c2cc(N3CCNC3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493663	187379	0	None	-	1	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	2	6	-0.7	CN1CCN(c2cc(N3CCNC3=O)nc(N)n2)CC1	10.1021/jm8005959
	127051986	140279	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806098	140279	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	51354301	58862	0	None	-1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	CHEMBL1688969	58862	0	None	-1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	10934158	108550	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL320331	108550	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	10017821	103759	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL308913	103759	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1238	2069	13	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	6603865	2069	13	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL11919	2069	13	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	11043445	56449	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632410	56449	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	10945113	109602	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL322256	109602	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	44480707	5731	0	None	5	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	328	8	3	4	2.2	N#C/N=C(/NCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078529	5731	0	None	5	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	328	8	3	4	2.2	N#C/N=C(/NCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	10945113	109602	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL322256	109602	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	127052294	140277	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	140277	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1247	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1375	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	774	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	CHEMBL90	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	DB05381	1919	119	None	-3	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	10934158	108550	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL320331	108550	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	25129182	187259	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	291	2	1	7	-0.4	CN1CCN(c2cc(N3CCN(C)CC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492869	187259	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	291	2	1	7	-0.4	CN1CCN(c2cc(N3CCN(C)CC3)nc(N)n2)CC1	10.1021/jm8005959
	127052293	140213	3	None	10	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	140213	3	None	10	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11846634	77980	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209758	77980	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	11846464	78431	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	CHEMBL211170	78431	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	25071319	189038	14	None	1	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	189038	14	None	1	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	44482030	5643	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	338	9	3	3	2.9	CC(CCN/C(=N\C#N)NCCCCc1c[nH]cn1)c1ccccc1	10.1021/jm900526h
	CHEMBL1077860	5643	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	338	9	3	3	2.9	CC(CCN/C(=N\C#N)NCCCCc1c[nH]cn1)c1ccccc1	10.1021/jm900526h
	25131561	187380	0	None	-3	2	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493664	187380	0	None	-3	2	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	135897837	187604	13	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	209	1	2	6	-0.5	CN1CCN(c2cc(O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494909	187604	13	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	209	1	2	6	-0.5	CN1CCN(c2cc(O)nc(N)n2)CC1	10.1021/jm8005959
	127048828	140295	0	None	38	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806255	140295	0	None	38	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227255	117099	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393539	117099	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	44568832	187159	0	None	-	1	Rat	7.8	pEC50	=	7.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	2	1	6	1.2	CN1CCN(c2cc(-c3ccc(F)nc3F)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492242	187159	0	None	-	1	Rat	7.8	pEC50	=	7.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	2	1	6	1.2	CN1CCN(c2cc(-c3ccc(F)nc3F)nc(N)n2)CC1	10.1021/jm8005959
	25128822	187457	0	None	19	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	187457	0	None	19	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	1251	2001	39	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	3035842	2001	39	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	CHEMBL18661	2001	39	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	1251	2001	39	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	3035842	2001	39	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL18661	2001	39	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	25149712	178962	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	299	7	4	3	1.6	N=C(NCCCc1c[nH]cn1)NC(=O)CCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL470790	178962	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	299	7	4	3	1.6	N=C(NCCCc1c[nH]cn1)NC(=O)CCc1ccccc1	10.1016/j.bmcl.2010.10.041
	25149335	184201	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL483406	184201	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	1278	2137	84	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2137	84	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2137	84	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2137	84	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	127048890	140270	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805995	140270	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	46890548	6552	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083084	6552	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44481761	5742	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	324	9	3	3	2.4	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccc1	10.1021/jm900526h
	CHEMBL1078631	5742	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	324	9	3	3	2.4	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccc1	10.1021/jm900526h
	51354301	58862	0	None	-1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688969	58862	0	None	-1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	155513612	176296	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	1204	1919	119	None	-288	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-288	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-288	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-288	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-288	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-288	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1248	1412	28	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	3077	1412	28	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	90045	1412	28	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	CHEMBL12344	1412	28	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	5310982	93583	4	None	-3	2	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	CHEMBL24665	93583	4	None	-3	2	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	1269	121	41	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	41	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	41	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	53233802	61517	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770987	61517	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	25131559	187561	0	None	23	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494667	187561	0	None	23	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	1204	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	44480959	5644	2	None	1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	206	5	3	3	0.1	N#C/N=C(\N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077861	5644	2	None	1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	206	5	3	3	0.1	N#C/N=C(\N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	1484519	173872	60	None	-	1	Rat	4.8	pEC50	=	4.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	227	1	1	5	0.5	CN1CCN(c2cc(Cl)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL454007	173872	60	None	-	1	Rat	4.8	pEC50	=	4.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	227	1	1	5	0.5	CN1CCN(c2cc(Cl)nc(N)n2)CC1	10.1021/jm8005959
	21638785	188634	5	None	1	2	Mouse	7.8	pEC50	=	7.8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188634	5	None	1	2	Mouse	7.8	pEC50	=	7.8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	127051958	140253	0	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805813	140253	0	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25129880	193106	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	2	1	5	2.6	CN1CCN(c2cc(-c3ccc4ccccc4c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL522841	193106	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	2	1	5	2.6	CN1CCN(c2cc(-c3ccc4ccccc4c3)nc(N)n2)CC1	10.1021/jm8005959
	25130231	187680	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	309	2	1	7	1.2	CN1CCN(c2cc(-n3cnc4ccccc43)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495280	187680	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	309	2	1	7	1.2	CN1CCN(c2cc(-n3cnc4ccccc43)nc(N)n2)CC1	10.1021/jm8005959
	25131228	187570	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL494723	187570	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25128828	188228	2	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	237	4	2	5	1.3	CN(C)CCNc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL499631	188228	2	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	237	4	2	5	1.3	CN(C)CCNc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	58227163	176439	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25071319	189038	14	None	-1	2	Mouse	7.7	pEC50	=	7.7	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	189038	14	None	-1	2	Mouse	7.7	pEC50	=	7.7	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1204	1919	119	None	-3	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	1247	1919	119	None	-3	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	1375	1919	119	None	-3	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	774	1919	119	None	-3	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	CHEMBL90	1919	119	None	-3	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	DB05381	1919	119	None	-3	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	25130233	170589	7	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3cn[nH]c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL445091	170589	7	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3cn[nH]c3)nc(N)n2)CC1	10.1021/jm8005959
	25128831	172589	0	None	-1	2	Rat	4.7	pEC50	=	4.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL449157	172589	0	None	-1	2	Rat	4.7	pEC50	=	4.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	25130574	187352	0	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(N)nc(-c3ccc(C#N)cc3)c2)CC1	10.1021/jm8005959
	CHEMBL493461	187352	0	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(N)nc(-c3ccc(C#N)cc3)c2)CC1	10.1021/jm8005959
	44568790	171390	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	2	2	5	1.4	CN[C@@H]1CCN(c2nc(N)nc3c2Cc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL446269	171390	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	2	2	5	1.4	CN[C@@H]1CCN(c2nc(N)nc3c2Cc2ccccc2-3)C1	10.1021/jm800670r
	155552061	176454	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	45257133	199881	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	199881	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	11846300	80237	3	None	-2	2	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	80237	3	None	-2	2	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	1204	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3032915	15463	24	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL12160	15463	24	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	53321919	56448	0	None	-50	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632409	56448	0	None	-50	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10060213	102884	1	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL306077	102884	1	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	25128823	192693	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192693	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	25131230	193085	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	329	4	1	7	1.5	COc1cccc(OC)c1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL522679	193085	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	329	4	1	7	1.5	COc1cccc(OC)c1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	10037650	5177	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106158	5177	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	170389	206958	35	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL4634086	206958	35	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL91353	206958	35	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	155513612	176296	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	136141537	56451	3	None	-1	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56451	3	None	-1	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	136192874	56453	0	None	-3	3	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56453	0	None	-3	3	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	46228133	200031	0	None	-	1	Human	6.7	pEC50	=	6.7	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595639	200031	0	None	-	1	Human	6.7	pEC50	=	6.7	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10197870	5269	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106612	5269	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10197870	5269	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106612	5269	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10879463	5258	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106570	5258	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	1278	2137	84	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2137	84	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2137	84	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2137	84	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1278	2137	84	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2137	84	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2137	84	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2137	84	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	58227260	117106	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393546	117106	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	11846636	77923	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209628	77923	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	1204	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1247	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1375	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	774	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	CHEMBL90	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	DB05381	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	16061961	199656	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	199656	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	1204	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	1247	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	1375	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	774	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	CHEMBL90	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	DB05381	1919	119	None	-3	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	127052635	140182	0	None	4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805003	140182	0	None	4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130237	187258	2	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	1	1	5	0.4	CN1CCN(c2cc(I)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492864	187258	2	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	1	1	5	0.4	CN1CCN(c2cc(I)nc(N)n2)CC1	10.1021/jm8005959
	127051301	140175	0	None	4	3	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	140175	0	None	4	3	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25131555	187155	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3cccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492227	187155	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3cccnc3)nc(N)n2)CC1	10.1021/jm8005959
	25130914	187230	0	None	15	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL492677	187230	0	None	15	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10879463	5258	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106570	5258	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	1613	2333	53	None	-26	34	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	205	2333	53	None	-26	34	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	3964	2333	53	None	-26	34	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	CHEMBL831	2333	53	None	-26	34	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	DB00408	2333	53	None	-26	34	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	753256	179326	12	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL473662	179326	12	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1021/jm8005959
	25131560	187322	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cnn(C)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493229	187322	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cnn(C)c3)nc(N)n2)CC1	10.1021/jm8005959
	1223	950	41	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	2790	950	41	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	CHEMBL14690	950	41	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	155512725	176217	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	10037650	5177	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106158	5177	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	25129881	187158	5	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3ccncc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492241	187158	5	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3ccncc3)nc(N)n2)CC1	10.1021/jm8005959
	44568855	193170	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	285	2	1	5	2.0	CC(C)(C)c1cc(N2CCNCC2)nc(-n2cccc2)n1	10.1021/jm8005959
	CHEMBL523335	193170	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	285	2	1	5	2.0	CC(C)(C)c1cc(N2CCNCC2)nc(-n2cccc2)n1	10.1021/jm8005959
	25128832	171988	0	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	5	1.2	CN1CCN(c2cc(C(C)(C)C)nc(O)n2)CC1	10.1021/jm8005959
	CHEMBL447151	171988	0	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	5	1.2	CN1CCN(c2cc(C(C)(C)C)nc(O)n2)CC1	10.1021/jm8005959
	25071940	187330	0	None	-	1	Rat	7.6	pEC50	=	7.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	187330	0	None	-	1	Rat	7.6	pEC50	=	7.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	127049156	140169	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804837	140169	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	11437850	187637	1	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3cccc(C#N)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495082	187637	1	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3cccc(C#N)c3)nc(N)n2)CC1	10.1021/jm8005959
	25129883	192699	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ccncc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL522019	192699	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ccncc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25133972	187326	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(-c3ccc(C#N)cc3)cc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493262	187326	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(-c3ccc(C#N)cc3)cc(N)n2)CC1	10.1021/jm8005959
	25130575	187565	1	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.6	CC1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494687	187565	1	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.6	CC1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	25130577	172574	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	4	1	6	1.0	CN(C)CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL449021	172574	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	4	1	6	1.0	CN(C)CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	10773349	8059	1	None	-89	2	Human	4.6	pEC50	=	4.6	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL109130	8059	1	None	-89	2	Human	4.6	pEC50	=	4.6	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	127052634	140284	0	None	6	2	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806146	140284	0	None	6	2	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	90667240	109452	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	356	9	3	4	2.8	Cc1[nH]cnc1CCCCN/C(=N\C#N)NCCSc1ccccc1	10.1039/C3MD00245D
	CHEMBL3220642	109452	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	356	9	3	4	2.8	Cc1[nH]cnc1CCCCN/C(=N\C#N)NCCSc1ccccc1	10.1039/C3MD00245D
	25130576	187602	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187602	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25128826	188792	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.5	CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL506187	188792	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.5	CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	25130576	187602	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187602	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	58227157	115891	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354815	115891	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	1204	1919	119	None	-3	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1247	1919	119	None	-3	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1375	1919	119	None	-3	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	774	1919	119	None	-3	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	CHEMBL90	1919	119	None	-3	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	DB05381	1919	119	None	-3	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1226	2006	20	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	41376	2006	20	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	CHEMBL12608	2006	20	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	25133970	187564	2	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL494679	187564	2	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130230	172432	2	None	3	2	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL448085	172432	2	None	3	2	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	1269	121	41	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	41	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	41	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	44480958	5641	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccncc1	10.1021/jm900526h
	CHEMBL1077859	5641	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccncc1	10.1021/jm900526h
	25131556	188847	7	None	3	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL507063	188847	7	None	3	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	127051047	140286	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806162	140286	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	45257134	199920	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	CHEMBL594949	199920	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	25131231	187163	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(I)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492288	187163	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(I)cc3)nc(N)n2)CC1	10.1021/jm8005959
	10381810	205151	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL7828	205151	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	155512725	176217	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	127052294	140277	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	140277	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1919	119	None	-288	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-288	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-288	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-288	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-288	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-288	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25128822	187457	0	None	-19	2	Human	6.5	pEC50	=	6.5	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL494093	187457	0	None	-19	2	Human	6.5	pEC50	=	6.5	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	155491015	176471	4	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	176471	4	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	176471	4	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	46890072	6878	0	None	-	1	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084355	6878	0	None	-	1	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7284	0	None	3	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7284	0	None	3	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	58227373	176484	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	21638785	188634	5	None	-1	2	Rat	7.5	pEC50	=	7.5	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188634	5	None	-1	2	Rat	7.5	pEC50	=	7.5	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	25130911	185206	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	236	2	1	6	-0.1	CN1CCN(c2cc(N(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL485802	185206	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	236	2	1	6	-0.1	CN1CCN(c2cc(N(C)C)nc(N)n2)CC1	10.1021/jm8005959
	16352	117159	13	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL339414	117159	13	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	1267	3775	49	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	25130579	187688	2	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	238	4	1	5	1.3	CN(C)CCOc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL495341	187688	2	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	238	4	1	5	1.3	CN(C)CCOc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	10678661	5330	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106931	5330	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	46228101	199775	0	None	-	1	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594022	199775	0	None	-	1	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	1249	1996	16	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	13499360	1996	16	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90019	1996	16	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1269	121	41	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	41	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	41	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	25129528	187162	0	None	-	1	Rat	7.5	pEC50	=	7.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(Cl)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492287	187162	0	None	-	1	Rat	7.5	pEC50	=	7.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(Cl)c3)nc(N)n2)CC1	10.1021/jm8005959
	44568923	193079	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	327	2	1	5	2.3	CN1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL522661	193079	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	327	2	1	5	2.3	CN1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)CC1	10.1021/jm8005959
	67087450	117110	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	117110	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	44547127	115892	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115892	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227148	117107	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	117107	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	10678661	5330	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106931	5330	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	25131557	193235	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	8	0.3	COc1ncc(-c2cc(N3CCN(C)CC3)nc(N)n2)cn1	10.1021/jm8005959
	CHEMBL523835	193235	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	8	0.3	COc1ncc(-c2cc(N3CCN(C)CC3)nc(N)n2)cn1	10.1021/jm8005959
	25130909	187356	0	None	1	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	CHEMBL493478	187356	0	None	1	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	44478385	5744	6	None	20	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078642	5744	6	None	20	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	46890549	6553	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083085	6553	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44480960	5598	2	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	248	6	3	3	1.2	CC(C)N/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077636	5598	2	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	248	6	3	3	1.2	CC(C)N/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	42625303	180742	0	None	-2089	6	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180742	0	None	-2089	6	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	56954425	82052	0	None	-13	2	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	584	23	6	4	4.0	N/C(=N\C(=O)CCCCCCCCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165643	82052	0	None	-13	2	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	584	23	6	4	4.0	N/C(=N\C(=O)CCCCCCCCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	25130913	170163	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	278	2	1	7	-0.4	CN1CCN(c2cc(N3CCOCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL444484	170163	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	278	2	1	7	-0.4	CN1CCN(c2cc(N3CCOCC3)nc(N)n2)CC1	10.1021/jm8005959
	1274	4033	16	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	4033	16	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	4033	16	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	155517880	176643	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	176454	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	25129180	187227	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	4	1	6	0.7	CCN(CC)c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL492662	187227	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	4	1	6	0.7	CCN(CC)c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	3228743	187357	5	None	-	1	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL493479	187357	5	None	-	1	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	12769844	109451	0	None	-3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	234	5	3	3	0.7	CN/C(=N/C#N)NCCCCc1nc[nH]c1C	10.1039/C3MD00245D
	CHEMBL3220641	109451	0	None	-3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	234	5	3	3	0.7	CN/C(=N/C#N)NCCCCc1nc[nH]c1C	10.1039/C3MD00245D
	25129181	187228	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	305	3	1	7	-0.0	CN1CCN(c2cc(N3CCC(N(C)C)C3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492663	187228	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	305	3	1	7	-0.0	CN1CCN(c2cc(N3CCC(N(C)C)C3)nc(N)n2)CC1	10.1021/jm8005959
	25128825	187540	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.2	CN1CCC(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494585	187540	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.2	CN1CCC(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	44478385	5744	6	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/acs.jmedchem.6b00120
	CHEMBL1078642	5744	6	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/acs.jmedchem.6b00120
	44478385	5744	6	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1039/C3MD00245D
	CHEMBL1078642	5744	6	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1039/C3MD00245D
	25129527	187390	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493720	187390	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm8005959
	24798596	199844	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594491	199844	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	127052292	140241	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805663	140241	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1274	4033	16	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	4033	16	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	4033	16	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	11846465	141907	0	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	CHEMBL387054	141907	0	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	25131559	187561	0	None	-23	2	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494667	187561	0	None	-23	2	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	25131561	187380	0	None	3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493664	187380	0	None	3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	46235428	6572	0	None	-263	3	Mouse	7.4	pEC50	=	7.4	Functional	Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6572	0	None	-263	3	Mouse	7.4	pEC50	=	7.4	Functional	Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1269	121	41	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	41	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	41	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	136030655	56452	0	None	-3	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56452	0	None	-3	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25129523	187263	45	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492884	187263	45	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25129523	187263	45	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	187263	45	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	70692530	75662	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL2047474	75662	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	91613	17955	34	None	-5	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL12620	17955	34	None	-5	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	58227326	117102	0	None	-	1	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393542	117102	0	None	-	1	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	58227373	176484	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	25071004	192694	0	None	1	2	Mouse	8.4	pEC50	=	8.4	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192694	0	None	1	2	Mouse	8.4	pEC50	=	8.4	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	42625303	180742	0	None	-2	6	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180742	0	None	-2	6	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	25128824	193154	0	None	1	2	Mouse	8.3	pEC50	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	193154	0	None	1	2	Mouse	8.3	pEC50	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	10702196	108541	1	None	-1	2	Human	4.4	pEC50	=	4.4	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL320279	108541	1	None	-1	2	Human	4.4	pEC50	=	4.4	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	56954320	82050	0	None	-10	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	472	15	6	4	0.9	N/C(=N\C(=O)CCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165641	82050	0	None	-10	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	472	15	6	4	0.9	N/C(=N\C(=O)CCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	1267	3775	49	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	3035905	3775	49	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	CHEMBL260374	3775	49	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	25129524	187160	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	CHEMBL492274	187160	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	25130909	187356	0	None	-1	2	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	CHEMBL493478	187356	0	None	-1	2	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	1278	2137	84	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2137	84	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2137	84	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2137	84	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	25128833	188378	38	None	2	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	CHEMBL501882	188378	38	None	2	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	25129526	187389	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL493719	187389	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	24797248	199843	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594490	199843	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	127050434	140225	0	None	3	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	160	3	4	3	0.2	CC(=N)SCCNC(=N)N	10.1021/acs.jmedchem.6b00120
	CHEMBL3805514	140225	0	None	3	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	160	3	4	3	0.2	CC(=N)SCCNC(=N)N	10.1021/acs.jmedchem.6b00120
	25129169	187157	0	None	1	2	Mouse	7.3	pEC50	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	187157	0	None	1	2	Mouse	7.3	pEC50	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	24798595	170352	2	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	276	2	1	6	0.8	CN1CCN(c2cc(N3CCCCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL444782	170352	2	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	276	2	1	6	0.8	CN1CCN(c2cc(N3CCCCC3)nc(N)n2)CC1	10.1021/jm8005959
	90667239	109450	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	8	3	4	2.5	Cc1nc(CCCN/C(=N\C#N)NCCSc2ccccc2)c[nH]1	10.1039/C3MD00245D
	CHEMBL3220640	109450	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	8	3	4	2.5	Cc1nc(CCCN/C(=N\C#N)NCCSc2ccccc2)c[nH]1	10.1039/C3MD00245D
	25130573	186861	2	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL489663	186861	2	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130581	187201	0	None	-3	2	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL492489	187201	0	None	-3	2	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	25130235	187563	1	None	-4	3	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187563	1	None	-4	3	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	46890546	6483	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6483	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228143	200060	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595880	200060	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	117724523	117089	2	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393529	117089	2	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	25130914	187230	0	None	-15	2	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492677	187230	0	None	-15	2	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25129179	187644	4	None	-	1	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	222	2	2	6	-0.1	CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL495112	187644	4	None	-	1	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	222	2	2	6	-0.1	CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25130236	187677	0	None	1	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	CHEMBL495265	187677	0	None	1	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	25068753	188414	19	None	1	2	Mouse	8.2	pEC50	=	8.2	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	188414	19	None	1	2	Mouse	8.2	pEC50	=	8.2	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	53233805	61494	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770964	61494	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	46890545	7353	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086460	7353	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1274	4033	16	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	3063228	4033	16	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	CHEMBL1196470	4033	16	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	1274	4033	16	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	3063228	4033	16	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	CHEMBL1196470	4033	16	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	136141537	56451	3	None	-5	3	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56451	3	None	-5	3	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	11313837	2483	8	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1254	2483	8	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL175782	2483	8	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	10583206	109333	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321920	109333	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-151	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-151	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-151	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-151	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-151	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-151	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	155513612	176296	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155512725	176217	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	58227163	176439	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25130580	174278	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.6	CNc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL455016	174278	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.6	CNc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	25129878	187311	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	324	3	1	7	1.4	COc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL493103	187311	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	324	3	1	7	1.4	COc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	127052295	140172	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	352	7	3	4	2.4	N#C/N=C(/NCCOc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804862	140172	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	352	7	3	4	2.4	N#C/N=C(/NCCOc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1919	119	None	-288	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-288	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-288	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-288	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-288	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-288	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	53233803	61492	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770962	61492	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	25129169	187157	0	None	-1	2	Rat	7.3	pEC50	=	7.3	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	187157	0	None	-1	2	Rat	7.3	pEC50	=	7.3	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	127049856	140220	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805431	140220	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	90667238	109449	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	220	4	3	3	0.3	CN/C(=N/C#N)NCCCc1c[nH]c(C)n1	10.1039/C3MD00245D
	CHEMBL3220639	109449	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	220	4	3	3	0.3	CN/C(=N/C#N)NCCCc1c[nH]c(C)n1	10.1039/C3MD00245D
	565544	109716	11	None	-4466	2	Human	6.3	pEC50	=	6.3	Functional	Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor	ChEMBL	125	2	2	2	0.5	CC(CN)c1cnc[nH]1	10.1021/jm0300025
	CHEMBL322988	109716	11	None	-4466	2	Human	6.3	pEC50	=	6.3	Functional	Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor	ChEMBL	125	2	2	2	0.5	CC(CN)c1cnc[nH]1	10.1021/jm0300025
	10583206	109333	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321920	109333	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	25130915	187264	1	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL492885	187264	1	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	46228125	199965	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595215	199965	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	44482028	5592	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccn1	10.1021/jm900526h
	CHEMBL1077580	5592	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccn1	10.1021/jm900526h
	25131232	187266	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	308	2	1	6	1.7	Cc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL492900	187266	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	308	2	1	6	1.7	Cc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	25130235	187563	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187563	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187563	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187563	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	45257133	199881	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	199881	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25128824	193154	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	193154	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	155517880	176643	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	45269820	195326	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202326	195326	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL551790	195326	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	25071004	192694	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192694	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	46890022	7014	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	8	3	4	4.3	Cc1cc(OCCCNCC2CCCNC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084869	7014	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	8	3	4	4.3	Cc1cc(OCCCNCC2CCCNC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228158	202135	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL609289	202135	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890547	6484	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082772	6484	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	1255	3173	19	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	6421522	3173	19	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL19173	3173	19	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	9974306	204451	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72372	204451	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	10060212	204511	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72769	204511	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	136192874	56453	0	None	1	3	Mouse	7.2	pEC50	=	7.2	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56453	0	None	1	3	Mouse	7.2	pEC50	=	7.2	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1236	2054	25	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1237	2054	25	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	156615	2054	25	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL268229	2054	25	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	25129175	187355	0	None	-	1	Rat	6.2	pEC50	=	6.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL493477	187355	0	None	-	1	Rat	6.2	pEC50	=	6.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	25130914	187230	0	None	15	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492677	187230	0	None	15	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25130908	171827	2	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL446896	171827	2	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	24797775	187221	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492631	187221	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	1204	1919	119	None	-151	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-151	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-151	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-151	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-151	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-151	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25130912	188715	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL505061	188715	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1021/jm8005959
	1204	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25149521	179028	0	None	9	2	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL471413	179028	0	None	9	2	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1247	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1375	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	774	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	CHEMBL90	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	DB05381	1919	119	None	-3	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1267	3775	49	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3775	49	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3775	49	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	1269	121	41	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	37463	121	41	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	CHEMBL275443	121	41	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	1267	3775	49	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3775	49	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3775	49	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	44482031	5610	1	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	234	6	3	3	0.8	CCN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077756	5610	1	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	234	6	3	3	0.8	CCN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	46890019	7280	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7280	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	156015638	178248	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	1279	2137	84	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	4908365	2137	84	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	CHEMBL129198	2137	84	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	10978141	5118	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL105803	5118	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	58227127	117103	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393543	117103	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	44481489	5681	0	None	18	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(\NCCCc1ccccc1)NCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1078210	5681	0	None	18	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(\NCCCc1ccccc1)NCCCc1c[nH]cn1	10.1021/jm900526h
	10419556	5326	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106913	5326	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	11086114	7362	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL108649	7362	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10820930	5767	0	None	-	1	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL107877	5767	0	None	-	1	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10106063	109244	10	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321860	109244	10	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10106063	109244	10	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	CHEMBL321860	109244	10	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	56954318	82049	0	None	2	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	444	13	6	4	0.1	N/C(=N\C(=O)CCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165640	82049	0	None	2	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	444	13	6	4	0.1	N/C(=N\C(=O)CCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	10106063	109244	10	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321860	109244	10	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10017000	204462	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72431	204462	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-3	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-3	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-3	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-3	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-3	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	136192874	56453	0	None	-1	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56453	0	None	-1	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1267	3775	49	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	3035905	3775	49	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	CHEMBL260374	3775	49	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	1239	2020	24	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1240	2020	24	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	912	2020	24	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL268490	2020	24	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10978141	5118	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL105803	5118	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	1278	2137	84	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	1279	2137	84	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	4908365	2137	84	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	CHEMBL129198	2137	84	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	42625303	180742	0	None	-501	6	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180742	0	None	-501	6	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	10419556	5326	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106913	5326	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	11086114	7362	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL108649	7362	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	155513612	176296	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	25130230	172432	2	None	-3	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL448085	172432	2	None	-3	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	25133971	187325	2	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2nc(N)cc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL493261	187325	2	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2nc(N)cc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130229	188731	7	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	271	2	1	7	0.3	CN1CCN(c2cc(-c3cncnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL505346	188731	7	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	271	2	1	7	0.3	CN1CCN(c2cc(-c3cncnc3)nc(N)n2)CC1	10.1021/jm8005959
	56954515	82036	0	None	-2	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	546	17	6	6	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165628	82036	0	None	-2	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	546	17	6	6	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm201128q
	56954322	82051	0	None	-4	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	500	17	6	4	1.7	N/C(=N\C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165642	82051	0	None	-4	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	500	17	6	4	1.7	N/C(=N\C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	45257131	199885	0	None	-	1	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	199885	0	None	-	1	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	11846298	139256	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	CHEMBL379102	139256	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	25129176	172737	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2nc(N)ncc2-c2ccccc2)CC1	10.1021/jm8005959
	CHEMBL451108	172737	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2nc(N)ncc2-c2ccccc2)CC1	10.1021/jm8005959
	58227388	117098	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393538	117098	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	1223	950	41	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	2790	950	41	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	CHEMBL14690	950	41	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	10820929	108180	1	None	-30	2	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL319658	108180	1	None	-30	2	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	53233801	61515	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770985	61515	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	58227252	117104	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393544	117104	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	1204	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-151	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1274	4033	16	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	4033	16	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	4033	16	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	25130236	187677	0	None	-1	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	CHEMBL495265	187677	0	None	-1	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	1267	3775	49	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3775	49	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3775	49	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	25129877	169436	0	None	-	1	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(I)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL442865	169436	0	None	-	1	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(I)c3)nc(N)n2)CC1	10.1021/jm8005959
	44568847	187225	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	6	2.0	CN1CCN(c2cc(Sc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492652	187225	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	6	2.0	CN1CCN(c2cc(Sc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25101945	193082	0	None	1	2	Mouse	8.0	pEC50	=	8.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	193082	0	None	1	2	Mouse	8.0	pEC50	=	8.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	25101945	193082	0	None	-1	2	Rat	8.0	pEC50	=	8.0	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	193082	0	None	-1	2	Rat	8.0	pEC50	=	8.0	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	58227143	117101	1	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393541	117101	1	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	16061961	199656	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	199656	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	112936251	137241	1	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752085	137241	1	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	58227163	176439	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25131556	188847	7	None	-3	2	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL507063	188847	7	None	-3	2	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25128830	187689	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	234	1	0	4	1.5	CN1CCN(c2cc(C(C)(C)C)ncn2)CC1	10.1021/jm8005959
	CHEMBL495342	187689	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	234	1	0	4	1.5	CN1CCN(c2cc(C(C)(C)C)ncn2)CC1	10.1021/jm8005959
	127035835	137260	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752270	137260	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	11983344	5645	5	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	220	5	3	3	0.4	CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077862	5645	5	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	220	5	3	3	0.4	CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	25128829	187310	0	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	265	5	1	5	1.7	CN(C)CCCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL493100	187310	0	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	265	5	1	5	1.7	CN(C)CCCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	24745335	294	12	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	294	12	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	156013896	178216	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	44129134	199485	0	None	-	1	Human	8.0	pIC50	=	8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	199485	0	None	-	1	Human	8.0	pIC50	=	8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	56835171	69711	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69711	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	16082828	80231	0	None	-6	3	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213886	80231	0	None	-6	3	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	71525678	89696	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89696	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	71525679	89697	0	None	-	1	Human	6.9	pIC50	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89697	0	None	-	1	Human	6.9	pIC50	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	1278	2137	84	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	1279	2137	84	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	4908365	2137	84	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	CHEMBL129198	2137	84	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	10446295	2119	34	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2119	34	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2119	34	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	155563181	175279	0	None	-	1	Human	5.9	pIC50	=	5.9	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	498	12	1	7	5.1	CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1c1ccc(OCCCCCN2CCCCC2)cc1	10.1021/acs.jmedchem.9b00937
	CHEMBL4573309	175279	0	None	-	1	Human	5.9	pIC50	=	5.9	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	498	12	1	7	5.1	CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1c1ccc(OCCCCCN2CCCCC2)cc1	10.1021/acs.jmedchem.9b00937
	25147771	174214	0	None	-	1	Human	6.8	pIC50	=	6.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	174214	0	None	-	1	Human	6.8	pIC50	=	6.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	16082833	80318	0	None	-40	2	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214312	80318	0	None	-40	2	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	90645145	112151	0	None	-	1	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290578	112151	0	None	-	1	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1267	3775	49	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	3035905	3775	49	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	CHEMBL260374	3775	49	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	1278	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1278	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	1279	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	4908365	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	CHEMBL129198	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	3201696	15737	4	None	-22	2	Human	4.7	pIC50	=	4.7	Functional	Antagonist activity at human histamine H4 receptorAntagonist activity at human histamine H4 receptor	ChEMBL	348	5	0	6	2.1	CN1CCN(C(c2ccccc2)c2nnnn2Cc2ccccc2)CC1	10.1016/j.bmcl.2010.07.009
	CHEMBL1222728	15737	4	None	-22	2	Human	4.7	pIC50	=	4.7	Functional	Antagonist activity at human histamine H4 receptorAntagonist activity at human histamine H4 receptor	ChEMBL	348	5	0	6	2.1	CN1CCN(C(c2ccccc2)c2nnnn2Cc2ccccc2)CC1	10.1016/j.bmcl.2010.07.009
	1278	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	63868328	174227	2	None	-	1	Human	6.6	pIC50	=	6.6	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4549015	174227	2	None	-	1	Human	6.6	pIC50	=	6.6	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71548412	1896	20	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1896	20	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1896	20	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	16656871	7944	0	None	-	1	Human	6.5	pIC50	=	6.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090531	7944	0	None	-	1	Human	6.5	pIC50	=	6.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	16718567	8130	0	None	3311	2	Human	8.5	pIC50	=	8.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091874	8130	0	None	3311	2	Human	8.5	pIC50	=	8.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2137	84	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	155540438	172517	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4483783	172517	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	44129130	199533	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	199533	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11682208	8242	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092542	8242	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	25112105	8131	0	None	-	1	Human	8.4	pIC50	=	8.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091875	8131	0	None	-	1	Human	8.4	pIC50	=	8.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	24745335	294	12	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	1278	2137	84	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2137	84	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2137	84	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2137	84	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	16656872	7880	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090190	7880	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	122193095	123888	0	None	-	1	Human	6.4	pIC50	=	6.4	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123888	0	None	-	1	Human	6.4	pIC50	=	6.4	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	49841846	69709	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69709	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	49841719	69708	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69708	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	1278	2137	84	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	90645147	112149	0	None	-	1	Human	6.3	pIC50	=	6.3	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290576	112149	0	None	-	1	Human	6.3	pIC50	=	6.3	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	24745335	294	12	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	294	12	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	294	12	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	294	12	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	10446295	2119	34	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2119	34	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2119	34	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	122194625	123995	0	None	-	1	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629828	123995	0	None	-	1	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	1267	3775	49	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	3035905	3775	49	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	CHEMBL260374	3775	49	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	1267	3775	49	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3035905	3775	49	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	CHEMBL260374	3775	49	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	25147772	173385	9	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	173385	9	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	71548412	1896	20	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1896	20	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1896	20	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	11709443	8214	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092332	8214	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71519674	89700	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89700	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	16656874	8074	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091569	8074	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	49841845	69710	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69710	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	156013896	178216	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2137	84	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2137	84	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2137	84	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	16082831	80317	0	None	-100	3	Human	6.1	pIC50	=	6.1	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL214311	80317	0	None	-100	3	Human	6.1	pIC50	=	6.1	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	71525681	89699	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89699	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	1267	3775	49	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3775	49	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3775	49	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	58227148	117107	0	None	-	1	Human	10.1	pKd	=	10.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	117107	0	None	-	1	Human	10.1	pKd	=	10.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	59777258	110106	0	None	-	0	Human	10.0	pKd	=	10	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236561	110106	0	None	-	0	Human	10.0	pKd	=	10	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	24797604	110097	2	None	-	0	Human	9.9	pKd	=	9.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	CHEMBL3236552	110097	2	None	-	0	Human	9.9	pKd	=	9.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	24994542	110099	0	None	-	0	Human	9.6	pKd	=	9.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236554	110099	0	None	-	0	Human	9.6	pKd	=	9.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	24798144	110098	2	None	-	1	Human	9.5	pKd	=	9.5	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	110098	2	None	-	1	Human	9.5	pKd	=	9.5	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	53257796	110120	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236575	110120	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	54587790	61510	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770980	61510	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	24745337	61509	0	None	-	0	Human	9.3	pKd	=	9.3	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770979	61509	0	None	-	0	Human	9.3	pKd	=	9.3	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	59777338	110101	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236556	110101	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	44547268	117086	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393526	117086	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227340	117092	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393532	117092	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	58227200	117095	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393535	117095	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	54585781	61531	0	None	-	0	Human	8.9	pKd	=	8.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771000	61531	0	None	-	0	Human	8.9	pKd	=	8.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	54583873	61499	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770969	61499	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54587787	61501	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770971	61501	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54581940	61522	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770992	61522	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25130912	188715	0	None	-	1	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL505061	188715	0	None	-	1	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	23653543	68019	2	None	-	0	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914541	68019	2	None	-	0	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	24994636	110110	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	CHEMBL3236565	110110	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	42624149	110113	1	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	110113	1	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	59777262	110117	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236572	110117	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	54584824	61507	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770977	61507	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227337	117113	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393553	117113	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	58227259	117116	0	None	-	1	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	117116	0	None	-	1	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	24798144	110098	2	None	-	1	Mouse	8.0	pKd	=	8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	110098	2	None	-	1	Mouse	8.0	pKd	=	8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	24826766	68072	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914750	68072	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	9944571	116991	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338937	116991	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	24994634	2129	31	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	53258370	110114	1	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236569	110114	1	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	24994635	110115	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236570	110115	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	54582865	61489	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770959	61489	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	54584825	61520	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770990	61520	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	11587279	125025	4	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL364400	125025	4	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	46890627	6557	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083098	6557	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740470	109771	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3233048	109771	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	11687917	14933	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	CHEMBL120854	14933	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	9966927	18843	5	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128252	18843	5	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	54581938	61502	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770972	61502	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	67087450	117110	0	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	117110	0	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	11594278	14658	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL120591	14658	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	9923162	18908	6	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128613	18908	6	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	11120826	18886	1	None	-	0	Human	6.8	pKd	=	6.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128505	18886	1	None	-	0	Human	6.8	pKd	=	6.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	46890677	7259	0	None	-	0	Human	6.8	pKd	=	6.8	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086038	7259	0	None	-	0	Human	6.8	pKd	=	6.8	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7284	0	None	-3	4	Mouse	4.8	pKd	=	4.8	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7284	0	None	-3	4	Mouse	4.8	pKd	=	4.8	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740898	110107	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	CHEMBL3236562	110107	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	10468601	195568	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202324	195568	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL555434	195568	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	53233904	61495	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770965	61495	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	10446295	2119	34	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2119	34	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2119	34	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	4242975	18810	9	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128088	18810	9	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	9900313	19392	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129444	19392	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	9839217	72490	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199062	72490	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	11565330	97191	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	CHEMBL268525	97191	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	4906599	168638	11	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL436381	168638	11	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	24996854	110109	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	CHEMBL3236564	110109	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	42624150	110122	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236577	110122	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	24797956	110103	3	None	-	1	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	110103	3	None	-	1	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	24992735	110128	0	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	CHEMBL3236582	110128	0	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	10244243	141288	3	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL383384	141288	3	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	46890592	6360	0	None	-	2	Human	7.6	pKd	=	7.6	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6360	0	None	-	2	Human	7.6	pKd	=	7.6	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44547127	115892	0	None	-	1	Human	8.5	pKd	=	8.5	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115892	0	None	-	1	Human	8.5	pKd	=	8.5	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	24992077	110116	0	None	-	0	Human	8.4	pKd	=	8.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236571	110116	0	None	-	0	Human	8.4	pKd	=	8.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	10934567	19226	1	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL129026	19226	1	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	11630326	72542	0	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199231	72542	0	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	44547567	117093	2	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393533	117093	2	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	4060730	19347	56	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129199	19347	56	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	53378711	110123	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236578	110123	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	24994634	2129	31	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	54753437	68154	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915048	68154	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	42624149	110113	1	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	110113	1	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	46890590	6992	0	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6992	0	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11572877	67050	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL187285	67050	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	9992865	114400	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL333220	114400	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	11601630	132792	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL370135	132792	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	46890546	6483	0	None	-	1	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6483	0	None	-	1	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890590	6992	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6992	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	2063135	8346	9	None	-	1	Human	8.2	pKd	=	8.2	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1093180	8346	9	None	-	1	Human	8.2	pKd	=	8.2	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	11066922	19391	1	None	-	0	Human	8.2	pKd	=	8.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129436	19391	1	None	-	0	Human	8.2	pKd	=	8.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	24994634	2129	31	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	10083188	140909	3	None	-	0	Human	7.2	pKd	=	7.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL382276	140909	3	None	-	0	Human	7.2	pKd	=	7.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	46890019	7280	0	None	-	1	Human	7.2	pKd	=	7.2	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7280	0	None	-	1	Human	7.2	pKd	=	7.2	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1278	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	10967174	19410	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19410	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	42624148	110119	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236574	110119	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	54583875	61525	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770995	61525	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227179	117108	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393548	117108	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	58227086	117117	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393557	117117	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2137	84	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	10967174	19410	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129540	19410	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	10988959	118082	4	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL340740	118082	4	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	10923241	19133	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128958	19133	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	9878733	116818	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338051	116818	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	11623296	116997	0	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338972	116997	0	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	16202258	68189	0	None	-	0	Human	10.5	pKi	=	10.5	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915536	68189	0	None	-	0	Human	10.5	pKi	=	10.5	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	24745506	68190	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915537	68190	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	24745335	294	12	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	44187557	195476	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	CHEMBL1202332	195476	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	CHEMBL553423	195476	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	135525668	68183	0	None	-	0	Human	8.0	pKi	=	8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915348	68183	0	None	-	0	Human	8.0	pKi	=	8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	136042270	69886	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938979	69886	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24745507	68191	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915538	68191	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	57401167	69840	0	None	-	0	Human	6.9	pKi	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938846	69840	0	None	-	0	Human	6.9	pKi	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400778	68181	0	None	-	0	Human	6.8	pKi	=	6.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915346	68181	0	None	-	0	Human	6.8	pKi	=	6.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	135525668	68183	0	None	-	0	Human	7.8	pKi	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915348	68183	0	None	-	0	Human	7.8	pKi	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57391926	69873	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938967	69873	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042269	69885	0	None	-	0	Human	7.7	pKi	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938978	69885	0	None	-	0	Human	7.7	pKi	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57402929	69838	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938844	69838	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57395538	68182	0	None	-	0	Human	6.6	pKi	=	6.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915347	68182	0	None	-	0	Human	6.6	pKi	=	6.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042267	69883	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938976	69883	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400777	68180	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915345	68180	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	57400777	68180	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915345	68180	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	49841846	69709	0	None	-	1	Human	8.5	pKi	=	8.5	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69709	0	None	-	1	Human	8.5	pKi	=	8.5	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	11688307	69874	0	None	-	0	Human	5.5	pKi	=	5.5	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938968	69874	0	None	-	0	Human	5.5	pKi	=	5.5	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	54754351	68188	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915535	68188	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	57400778	68181	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915346	68181	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	10446295	2119	34	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	1277	2119	34	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL185951	2119	34	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	10446295	2119	34	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	1277	2119	34	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL185951	2119	34	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	49841719	69708	0	None	-	1	Human	8.4	pKi	=	8.4	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69708	0	None	-	1	Human	8.4	pKi	=	8.4	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	57391927	69875	0	None	-	0	Human	5.4	pKi	=	5.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938969	69875	0	None	-	0	Human	5.4	pKi	=	5.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1278	2137	84	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2137	84	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2137	84	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2137	84	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	49841845	69710	0	None	-	1	Human	8.3	pKi	=	8.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69710	0	None	-	1	Human	8.3	pKi	=	8.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	57395538	68182	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915347	68182	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	136042265	69881	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938974	69881	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57395620	68187	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915534	68187	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	57395620	68187	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915534	68187	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	1278	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1278	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1279	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	4908365	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL129198	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1278	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2137	84	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1267	3775	49	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3775	49	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3775	49	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	56835171	69711	0	None	-	1	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69711	0	None	-	1	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	57390145	69877	0	None	-	0	Human	5.1	pKi	=	5.1	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938970	69877	0	None	-	0	Human	5.1	pKi	=	5.1	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	136042268	69884	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938977	69884	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042266	69882	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938975	69882	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	54754351	68188	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915535	68188	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	56950378	69839	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938845	69839	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	135	2515	43	None	-2	33	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1796	2515	43	None	-2	33	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	4184	2515	43	None	-2	33	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	CHEMBL6437	2515	43	None	-2	33	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	DB06148	2515	43	None	-2	33	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1225	1459	26	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	3958	1459	26	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	667477	1459	26	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	CHEMBL860	1459	26	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	DB01142	1459	26	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	5344	173449	101	None	60	6	Human	8.3	pIC50	=	8.3	Functional	NoneNone	Drug Central	267	3	2	5	1.7	Cc1noc(NS(=O)(=O)c2ccc(N)cc2)c1C	None
	CHEMBL453	173449	101	None	60	6	Human	8.3	pIC50	=	8.3	Functional	NoneNone	Drug Central	267	3	2	5	1.7	Cc1noc(NS(=O)(=O)c2ccc(N)cc2)c1C	None
	1272	828	0	None	-	1	Human	8.1	pIC50	None	8.1	Functional	UnclassifiedUnclassified	Guide to Pharmacology		None	None	None		None	15265943

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Ligands					Receptor			Assay information							Chemical information
Sel. page	Common name	GPCRdb ID	#Vendors	Reference ligand	Fold selectivity (Affinity)	# tested GPCRs (Affinity)	Species	p-value (-log)	Type	Activity Relation	Activity Value	Assay Type	Assay Description	Source	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles	DOI
	51354301	58862	0	None	-10	2	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	CHEMBL1688969	58862	0	None	-10	2	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	70692530	75662	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL2047474	75662	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	90667434	109462	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	2	3	1.9	CC(CC(=O)/N=C(\N)NCCCn1ccnc1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL3220892	109462	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	2	3	1.9	CC(CC(=O)/N=C(\N)NCCCn1ccnc1)c1ccccc1	10.1039/C3MD00245D
	44367568	45711	5	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@@H]2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153051	45711	5	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@@H]2CCNC2)c[nH]1	10.1021/jm030905y
	9877454	205590	2	None	-4	2	Human	5.0	pEC50	=	5.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL81644	205590	2	None	-4	2	Human	5.0	pEC50	=	5.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	155512725	176217	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	1267	3775	49	None	-33	28	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	3035905	3775	49	None	-33	28	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	9793791	45988	3	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	137	2	2	2	0.5	NCC1CC1c1c[nH]cn1	10.1021/jm030905y
	CHEMBL153284	45988	3	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	137	2	2	2	0.5	NCC1CC1c1c[nH]cn1	10.1021/jm030905y
	155524115	176523	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	1204	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1247	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1375	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	774	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	DB05381	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1236	2054	25	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	1237	2054	25	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	156615	2054	25	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	CHEMBL268229	2054	25	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	46890592	6360	0	None	-	1	Mouse	5.9	pEC50	=	5.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6360	0	None	-	1	Mouse	5.9	pEC50	=	5.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7284	0	None	-4786	2	Mouse	7.9	pEC50	=	7.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7284	0	None	-4786	2	Mouse	7.9	pEC50	=	7.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1204	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1247	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1375	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	774	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	DB05381	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1204	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1247	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1375	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	774	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	DB05381	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1278	2137	84	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	1279	2137	84	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	4908365	2137	84	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	155524115	176523	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	176523	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	176523	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	11344458	205175	2	None	-14	2	Human	5.9	pEC50	=	5.9	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78497	205175	2	None	-14	2	Human	5.9	pEC50	=	5.9	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	1204	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1919	119	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	24797956	110103	3	None	-	1	Human	5.9	pEC50	=	5.9	Binding	Agonist activity at human H4 receptorAgonist activity at human H4 receptor	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	110103	3	None	-	1	Human	5.9	pEC50	=	5.9	Binding	Agonist activity at human H4 receptorAgonist activity at human H4 receptor	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	58227373	176484	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	176454	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	46887115	8479	0	None	-	0	Human	5.9	pEC50	=	5.9	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)C[C@H](C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1094144	8479	0	None	-	0	Human	5.9	pEC50	=	5.9	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)C[C@H](C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	58227373	176484	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155517880	176643	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	25149335	184201	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm9000693
	CHEMBL483406	184201	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm9000693
	25149335	184201	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL483406	184201	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1039/C3MD00245D
	25149335	184201	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm800841w
	CHEMBL483406	184201	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm800841w
	45267230	196459	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202318	196459	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL563342	196459	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	42625223	179901	0	None	-	1	Human	6.8	pEC50	=	6.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	395	8	4	3	1.4	CN(CCC(=O)/N=C(\N)NCCCc1c[nH]cn1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm9000693
	CHEMBL474617	179901	0	None	-	1	Human	6.8	pEC50	=	6.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	395	8	4	3	1.4	CN(CCC(=O)/N=C(\N)NCCCc1c[nH]cn1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm9000693
	11240759	105186	5	None	-190	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL311473	105186	5	None	-190	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	9812843	205221	1	None	-4	2	Human	4.8	pEC50	=	4.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78838	205221	1	None	-4	2	Human	4.8	pEC50	=	4.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	11469514	104141	2	None	-64	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL309654	104141	2	None	-64	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	155524115	176523	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	127052293	140213	3	None	-	4	Mouse	6.8	pEC50	=	6.8	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	140213	3	None	-	4	Mouse	6.8	pEC50	=	6.8	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	155513612	176296	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42626109	179744	0	None	-	1	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	324	6	4	2	1.5	N/C(=N\C(=O)Cc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL474447	179744	0	None	-	1	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	324	6	4	2	1.5	N/C(=N\C(=O)Cc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	155512725	176217	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	42625386	178329	0	None	-	1	Human	8.6	pEC50	=	8.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	6	3	2	0.6	CCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL465170	178329	0	None	-	1	Human	8.6	pEC50	=	8.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	6	3	2	0.6	CCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	46830167	8729	0	None	-	0	Human	8.6	pEC50	=	8.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096429	8729	0	None	-	0	Human	8.6	pEC50	=	8.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	11171320	45898	5	None	-	1	Human	5.7	pEC50	=	5.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	179	3	2	2	1.3	c1nc(CCC2CCCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153207	45898	5	None	-	1	Human	5.7	pEC50	=	5.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	179	3	2	2	1.3	c1nc(CCC2CCCNC2)c[nH]1	10.1021/jm030905y
	170389	206958	35	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	CHEMBL4634086	206958	35	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	CHEMBL91353	206958	35	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	46887041	8728	1	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096428	8728	1	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	155517880	176643	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	1204	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	127052293	140213	3	None	34	4	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	140213	3	None	34	4	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11301441	96125	10	None	-	1	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL260549	96125	10	None	-	1	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/acs.jmedchem.8b00435
	1278	2137	84	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	1279	2137	84	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	4908365	2137	84	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	CHEMBL129198	2137	84	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	58227401	176427	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42625222	179746	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	367	7	5	3	0.7	N/C(=N\C(=O)CNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL474449	179746	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	367	7	5	3	0.7	N/C(=N\C(=O)CNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	1267	3775	49	None	-21	28	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-21	28	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-21	28	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	90667228	109442	0	None	-	0	Human	5.5	pEC50	=	5.5	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL3220629	109442	0	None	-	0	Human	5.5	pEC50	=	5.5	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1ccccc1	10.1039/C3MD00245D
	58227401	176427	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42625303	180742	0	None	-	1	Human	8.5	pEC50	=	8.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL475621	180742	0	None	-	1	Human	8.5	pEC50	=	8.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	42625304	179076	0	None	-	1	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	5	3	2	0.4	CC(C)C(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL471724	179076	0	None	-	1	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	5	3	2	0.4	CC(C)C(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	11286909	205261	2	None	-1	2	Human	5.5	pEC50	=	5.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL79126	205261	2	None	-1	2	Human	5.5	pEC50	=	5.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	9964223	205664	11	None	-36	2	Human	4.5	pEC50	=	4.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL82244	205664	11	None	-36	2	Human	4.5	pEC50	=	4.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	42626111	189670	0	None	-	0	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	381	8	5	3	1.1	N/C(=N\C(=O)CCNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL515428	189670	0	None	-	0	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	381	8	5	3	1.1	N/C(=N\C(=O)CCNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	1204	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	58227373	176484	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	176454	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	58227163	176439	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	127050776	140222	0	None	-	2	Mouse	5.4	pEC50	=	5.4	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	140222	0	None	-	2	Mouse	5.4	pEC50	=	5.4	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1919	119	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	45269820	195326	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202326	195326	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL551790	195326	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	155512725	176217	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	16078981	44043	12	None	-	1	Human	5.4	pEC50	=	5.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	c1nc(CC2CCCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL151467	44043	12	None	-	1	Human	5.4	pEC50	=	5.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	c1nc(CC2CCCNC2)c[nH]1	10.1021/jm030905y
	155524115	176523	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	46887043	8731	0	None	-	0	Human	8.3	pEC50	=	8.3	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096431	8731	0	None	-	0	Human	8.3	pEC50	=	8.3	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	42625384	169749	0	None	-	1	Human	8.3	pEC50	=	8.3	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	209	4	3	2	-0.2	CC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL443896	169749	0	None	-	1	Human	8.3	pEC50	=	8.3	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	209	4	3	2	-0.2	CC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	44367567	169177	3	None	-	1	Human	6.4	pEC50	=	6.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@H]2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL440730	169177	3	None	-	1	Human	6.4	pEC50	=	6.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@H]2CCNC2)c[nH]1	10.1021/jm030905y
	58227163	176439	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	156013896	178216	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	127049855	140203	0	None	-1	4	Human	6.3	pEC50	=	6.3	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	140203	0	None	-1	4	Human	6.3	pEC50	=	6.3	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11957573	1997	34	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	1250	1997	34	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	3692	1997	34	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	CHEMBL19439	1997	34	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	155513612	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	127051301	140175	0	None	-	2	Mouse	6.3	pEC50	=	6.3	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	140175	0	None	-	2	Mouse	6.3	pEC50	=	6.3	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1919	119	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1247	1919	119	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1375	1919	119	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	774	1919	119	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90	1919	119	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	DB05381	1919	119	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	46235428	6572	0	None	-	1	Mouse	6.3	pEC50	=	6.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6572	0	None	-	1	Mouse	6.3	pEC50	=	6.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1251	2001	39	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	3035842	2001	39	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	CHEMBL18661	2001	39	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	1251	2001	39	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	3035842	2001	39	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	CHEMBL18661	2001	39	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	11206058	104388	9	None	-10	2	Human	4.2	pEC50	=	4.2	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL310360	104388	9	None	-10	2	Human	4.2	pEC50	=	4.2	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	127049855	140203	0	None	-	4	Mouse	6.2	pEC50	=	6.2	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	140203	0	None	-	4	Mouse	6.2	pEC50	=	6.2	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25149708	188412	0	None	-	0	Human	8.2	pEC50	=	8.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	319	7	4	4	2.2	CC(CC(=O)NC(=N)NCCCc1c[nH]cn1)c1cccs1	10.1021/jm800841w
	CHEMBL502339	188412	0	None	-	0	Human	8.2	pEC50	=	8.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	319	7	4	4	2.2	CC(CC(=O)NC(=N)NCCCc1c[nH]cn1)c1cccs1	10.1021/jm800841w
	58227401	176427	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	46887079	8477	0	None	-	0	Human	5.2	pEC50	=	5.2	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)CC(C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1094142	8477	0	None	-	0	Human	5.2	pEC50	=	5.2	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)CC(C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	127051301	140175	0	None	5	2	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	140175	0	None	5	2	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130914	187230	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL492677	187230	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	156015638	178248	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	58227163	176439	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25149613	179176	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	382	8	4	5	2.7	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1nccs1	10.1021/jm800841w
	CHEMBL472441	179176	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	382	8	4	5	2.7	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1nccs1	10.1021/jm800841w
	136773074	157341	0	None	-	1	Human	5.2	pEC50	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4077914	157341	0	None	-	1	Human	5.2	pEC50	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	42625221	189564	0	None	-	1	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	338	7	4	2	1.9	N/C(=N\C(=O)CCc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL514641	189564	0	None	-	1	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	338	7	4	2	1.9	N/C(=N\C(=O)CCc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	24798144	110098	2	None	-19	3	Mouse	8.1	pEC50	=	8.1	Binding	Partial agonist activity at mouse histamine H4 receptorPartial agonist activity at mouse histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	110098	2	None	-19	3	Mouse	8.1	pEC50	=	8.1	Binding	Partial agonist activity at mouse histamine H4 receptorPartial agonist activity at mouse histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	10374457	28864	2	None	-	2	Human	5.2	pEC50	=	5.2	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	123	1	2	2	0.2	N[C@H]1C[C@@H]1c1c[nH]cn1	10.1021/jm030905y
	CHEMBL13795	28864	2	None	-	2	Human	5.2	pEC50	=	5.2	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	123	1	2	2	0.2	N[C@H]1C[C@@H]1c1c[nH]cn1	10.1021/jm030905y
	11819221	45730	3	None	-	1	Human	6.1	pEC50	=	6.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(CC2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153061	45730	3	None	-	1	Human	6.1	pEC50	=	6.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(CC2CCNC2)c[nH]1	10.1021/jm030905y
	1204	1919	119	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	1247	1919	119	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	1375	1919	119	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	774	1919	119	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	CHEMBL90	1919	119	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	DB05381	1919	119	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	90667229	109443	0	None	-	0	Human	6.1	pEC50	=	6.1	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	319	7	3	3	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1cccs1	10.1039/C3MD00245D
	CHEMBL3220630	109443	0	None	-	0	Human	6.1	pEC50	=	6.1	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	319	7	3	3	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1cccs1	10.1039/C3MD00245D
	11546127	95455	2	None	-	1	Human	5.1	pEC50	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL25725	95455	2	None	-	1	Human	5.1	pEC50	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	182527	110541	8	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	167	4	4	2	-0.2	N=C(N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL325327	110541	8	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	167	4	4	2	-0.2	N=C(N)NCCCc1c[nH]cn1	10.1021/jm9000693
	46887042	8730	0	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096430	8730	0	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	25149521	179028	0	None	-	1	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1021/jm800841w
	CHEMBL471413	179028	0	None	-	1	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1021/jm800841w
	155552061	176454	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	155517880	176643	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	1249	1996	16	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	13499360	1996	16	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	CHEMBL90019	1996	16	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	1249	1996	16	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	13499360	1996	16	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90019	1996	16	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	1204	1919	119	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	1247	1919	119	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	1375	1919	119	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	774	1919	119	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	CHEMBL90	1919	119	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	DB05381	1919	119	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	4024	2002	20	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	9989505	2002	20	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	CHEMBL82298	2002	20	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	1204	1919	119	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1919	119	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1919	119	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1919	119	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1919	119	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1919	119	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	16718567	8130	0	None	-	1	Human	9.0	pIC50	=	9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091874	8130	0	None	-	1	Human	9.0	pIC50	=	9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885297	8383	0	None	-	0	Human	6.0	pIC50	=	6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	318	1	1	6	2.5	CN1CCN(c2nc(O)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093438	8383	0	None	-	0	Human	6.0	pIC50	=	6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	318	1	1	6	2.5	CN1CCN(c2nc(O)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	17747460	61299	12	None	-	4	Human	5.0	pIC50	=	5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	640	14	0	6	7.8	O=c1c2ccccc2c(Cc2ccc(Cl)cc2)nn1C[C@H]1CCCN1CCCCc1ccc(OCCCN2CCCCCC2)cc1	10.1021/jm1013874
	CHEMBL1767164	61299	12	None	-	4	Human	5.0	pIC50	=	5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	640	14	0	6	7.8	O=c1c2ccccc2c(Cc2ccc(Cl)cc2)nn1C[C@H]1CCCN1CCCCc1ccc(OCCCN2CCCCCC2)cc1	10.1021/jm1013874
	46884945	8213	0	None	-	0	Human	5.0	pIC50	=	5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	330	1	0	5	3.6	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CC(C)N1C	10.1016/j.bmcl.2010.02.097
	CHEMBL1092331	8213	0	None	-	0	Human	5.0	pIC50	=	5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	330	1	0	5	3.6	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CC(C)N1C	10.1016/j.bmcl.2010.02.097
	71547931	183087	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3c3ncnn23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4794724	183087	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3c3ncnn23)C1	10.1021/acs.jmedchem.7b01855
	11335478	160941	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL4115693	160941	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	1224	1420	83	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	3100	1420	83	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	8980	1420	83	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	916	1420	83	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	CHEMBL657	1420	83	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	DB01075	1420	83	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	11709443	8214	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092332	8214	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71548405	182135	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	0	7	1.1	CN(C)C1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782441	182135	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	0	7	1.1	CN(C)C1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	46884998	7945	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	1	1	5	2.6	NC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090532	7945	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	1	1	5	2.6	NC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	25112105	8131	0	None	-	1	Human	7.9	pIC50	=	7.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091875	8131	0	None	-	1	Human	7.9	pIC50	=	7.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	71547681	182921	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.7	Clc1cnc2nc(N3CCNCC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4792733	182921	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.7	Clc1cnc2nc(N3CCNCC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	46885128	7938	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.2	CNC1(C)CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090519	7938	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.2	CNC1(C)CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885129	7939	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	1	5	3.2	Clc1ccc2oc3c(N4CC5CCCNC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090520	7939	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	1	5	3.2	Clc1ccc2oc3c(N4CC5CCCNC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	46885241	8243	0	None	-	0	Human	4.9	pIC50	=	4.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	2	1	5	3.8	CN[C@@H]1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092543	8243	0	None	-	0	Human	4.9	pIC50	=	4.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	2	1	5	3.8	CN[C@@H]1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	156013896	178216	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	16656869	8072	1	None	-	0	Human	5.8	pIC50	=	5.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.1	CNC1CCN(c2nc(C)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091559	8072	1	None	-	0	Human	5.8	pIC50	=	5.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.1	CNC1CCN(c2nc(C)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	124087	1377	114	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	7157	1377	114	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	814	1377	114	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	CHEMBL1172	1377	114	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	DB00967	1377	114	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	11566111	8314	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1CC2CCC1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092933	8314	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1CC2CCC1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71547932	182743	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3c3ncnn23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4790239	182743	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3c3ncnn23)CC1	10.1021/acs.jmedchem.7b01855
	13680692	183410	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	313	2	0	8	0.9	CN1CCN(c2nc3ccc([N+](=O)[O-])cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4798729	183410	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	313	2	0	8	0.9	CN1CCN(c2nc3ccc([N+](=O)[O-])cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885239	8162	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092064	8162	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2010.02.097
	71547762	182695	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.7	CN1CCN(c2nc3nc(Cl)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789645	182695	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.7	CN1CCN(c2nc3nc(Cl)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	2063135	8346	9	None	-	1	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093180	8346	9	None	-	1	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	11695388	8212	0	None	-	0	Human	5.7	pIC50	=	5.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.2	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CCN1C	10.1016/j.bmcl.2010.02.097
	CHEMBL1092330	8212	0	None	-	0	Human	5.7	pIC50	=	5.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.2	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CCN1C	10.1016/j.bmcl.2010.02.097
	9904236	14044	22	None	-	1	Human	5.7	pIC50	=	5.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL1197564	14044	22	None	-	1	Human	5.7	pIC50	=	5.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46884997	7688	0	None	-	0	Human	4.7	pIC50	=	4.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	0	5	3.2	CN(C)C1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088888	7688	0	None	-	0	Human	4.7	pIC50	=	4.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	0	5	3.2	CN(C)C1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1530	2169	50	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	3827	2169	50	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	7206	2169	50	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	CHEMBL534	2169	50	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	DB00920	2169	50	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	20889070	181848	6	None	-	0	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	302	1	0	6	1.7	CN1CCN(c2nc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778864	181848	6	None	-	0	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	302	1	0	6	1.7	CN1CCN(c2nc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	820836	102914	10	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	268	1	0	6	1.0	CN1CCN(c2nc3ccccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL306316	102914	10	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	268	1	0	6	1.0	CN1CCN(c2nc3ccccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	121237834	147334	0	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL3929465	147334	0	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46884996	7965	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	Clc1ccc2oc3c(N4CCN5CCCC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090655	7965	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	Clc1ccc2oc3c(N4CCN5CCCC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	71548223	181836	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	401	3	0	8	2.0	CN1CCN(c2nc3nc(OCC(F)(F)F)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778712	181836	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	401	3	0	8	2.0	CN1CCN(c2nc3nc(OCC(F)(F)F)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71547685	182966	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	294	1	0	8	0.3	CN1CCN(c2nc3ncc(C#N)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4793425	182966	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	294	1	0	8	0.3	CN1CCN(c2nc3ncc(C#N)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548412	1896	20	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1896	20	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1896	20	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	71547764	182329	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	328	1	0	8	0.9	CN1CCN(c2nc3nc(C#N)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4784683	182329	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	328	1	0	8	0.9	CN1CCN(c2nc3nc(C#N)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548498	182216	0	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	348	1	0	8	0.6	CN1CCN(c2nc3ncc(Br)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4783487	182216	0	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	348	1	0	8	0.6	CN1CCN(c2nc3ncc(Br)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548031	182884	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.4	CN1CCN(c2nc3ncc(C(F)(F)F)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4792352	182884	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.4	CN1CCN(c2nc3ncc(C(F)(F)F)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	16656871	7944	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090531	7944	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2137	84	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	1279	2137	84	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	4908365	2137	84	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	CHEMBL129198	2137	84	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	71548412	1896	20	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1896	20	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1896	20	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	71548319	183214	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	CN1CCCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4796300	183214	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	CN1CCCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885178	7681	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	336	2	1	5	3.2	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088859	7681	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	336	2	1	5	3.2	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2010.02.097
	71548224	182504	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	361	1	0	7	1.5	Cc1c(Br)cnc2nc(N3CCN(C)CC3)c3nncn3c12	10.1021/acs.jmedchem.7b01855
	CHEMBL4787243	182504	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	361	1	0	7	1.5	Cc1c(Br)cnc2nc(N3CCN(C)CC3)c3nncn3c12	10.1021/acs.jmedchem.7b01855
	71547599	182044	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3ncccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781409	182044	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3ncccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71547680	182803	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3cccnc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4791152	182803	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3cccnc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	11709905	8339	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	342	2	0	5	3.7	CN1CCN(c2nc(C3CC3)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093138	8339	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	342	2	0	5	3.7	CN1CCN(c2nc(C3CC3)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	16656872	7880	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090190	7880	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	71548027	181826	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	395	1	0	7	1.0	CN1CCN(c2nc3ncc(I)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778591	181826	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	395	1	0	7	1.0	CN1CCN(c2nc3ncc(I)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	3934857	8071	29	None	-	0	Human	7.4	pIC50	=	7.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.1	Cc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091558	8071	29	None	-	0	Human	7.4	pIC50	=	7.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.1	Cc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	71547282	183049	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1C	10.1021/acs.jmedchem.7b01855
	CHEMBL4794336	183049	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1C	10.1021/acs.jmedchem.7b01855
	1278	2137	84	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2137	84	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2137	84	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2137	84	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	89477685	183550	0	None	-	0	Human	5.4	pIC50	=	5.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	1	7	1.1	CN[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4800462	183550	0	None	-	0	Human	5.4	pIC50	=	5.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	1	7	1.1	CN[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	89477651	183424	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	315	1	0	7	1.2	CN1C[C@@H]2C[C@H]1CN2c1nc2ncc(Cl)cc2n2cnnc12	10.1021/acs.jmedchem.7b01855
	CHEMBL4798915	183424	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	315	1	0	7	1.2	CN1C[C@@H]2C[C@H]1CN2c1nc2ncc(Cl)cc2n2cnnc12	10.1021/acs.jmedchem.7b01855
	11638468	8315	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1C2CCC1CN(c1ncnc3c1oc1ccc(Cl)cc13)C2	10.1016/j.bmcl.2010.02.097
	CHEMBL1092934	8315	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1C2CCC1CN(c1ncnc3c1oc1ccc(Cl)cc13)C2	10.1016/j.bmcl.2010.02.097
	71547281	183263	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	2	0	7	1.5	CN(C)[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4796842	183263	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	2	0	7	1.5	CN(C)[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	1267	3775	49	None	-33	28	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	89477709	182670	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	1	7	0.9	Clc1cnc2nc(N3C[C@@H]4C[C@H]3CN4)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789385	182670	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	1	7	0.9	Clc1cnc2nc(N3C[C@@H]4C[C@H]3CN4)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547597	183107	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	283	1	0	7	0.7	Cc1cnc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4795091	183107	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	283	1	0	7	0.7	Cc1cnc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547848	183013	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	329	1	0	7	1.6	Clc1cnc2nc(N3CCN4CCCC4C3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4793974	183013	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	329	1	0	7	1.6	Clc1cnc2nc(N3CCN4CCCC4C3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547765	182464	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	3	0	8	1.5	CCOc1nc2nc(N3CCN(C)CC3)c3nncn3c2cc1Cl	10.1021/acs.jmedchem.7b01855
	CHEMBL4786816	182464	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	3	0	8	1.5	CCOc1nc2nc(N3CCN(C)CC3)c3nncn3c2cc1Cl	10.1021/acs.jmedchem.7b01855
	71547682	182178	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	275	1	1	7	0.5	NC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782945	182178	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	275	1	1	7	0.5	NC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	89477439	182052	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	290	2	1	8	0.1	CNC1CN(c2nc3ncc(Cl)cc3n3nnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781484	182052	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	290	2	1	8	0.1	CNC1CN(c2nc3ncc(Cl)cc3n3nnnc23)C1	10.1021/acs.jmedchem.7b01855
	58475282	181628	0	None	-	0	Human	6.2	pIC50	=	6.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	282	1	0	6	1.3	Cc1ccc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4776004	181628	0	None	-	0	Human	6.2	pIC50	=	6.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	282	1	0	6	1.3	Cc1ccc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71548411	182555	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	304	1	0	8	0.5	CN1CCN(c2nc3ncc(Cl)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4787983	182555	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	304	1	0	8	0.5	CN1CCN(c2nc3ncc(Cl)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	53380276	182780	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	0	5	2.3	CN1CCN(c2cc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4790726	182780	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	0	5	2.3	CN1CCN(c2cc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885181	8305	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	293	2	1	6	2.0	CNC1CCN(c2ncnc3c2oc2ccc(C#N)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092880	8305	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	293	2	1	6	2.0	CNC1CCN(c2ncnc3c2oc2ccc(C#N)cc23)C1	10.1016/j.bmcl.2010.02.097
	89477539	182686	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.9	N[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789543	182686	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.9	N[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	71515465	182120	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782303	182120	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	71547683	181780	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	1	0	7	1.2	CN1CCN(c2nc3ncc(Br)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778068	181780	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	1	0	7	1.2	CN1CCN(c2nc3ncc(Br)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL5087167	215051	0	None	-	2	Mouse	5.2	pIC50	=	5.2	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL		None	None	None		Cn1cc2c(-c3c(-c4ccc(F)cn4)nn4c3COC(C)(C)C4)ccnc2n1	10.6019/CHEMBL5058645
	89477445	182026	0	None	-	0	Human	6.1	pIC50	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	1	7	1.1	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781143	182026	0	None	-	0	Human	6.1	pIC50	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	1	7	1.1	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1	10.1021/acs.jmedchem.7b01855
	71547678	183316	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4797517	183316	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	24994634	2129	31	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2129	31	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2129	31	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	11682208	8242	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092542	8242	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	46885177	8097	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	282	2	1	5	2.5	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091729	8097	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	282	2	1	5	2.5	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2137	84	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2137	84	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2137	84	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2137	84	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	16656874	8074	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091569	8074	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885298	8384	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	332	2	0	6	2.8	COc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093439	8384	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	332	2	0	6	2.8	COc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	16656870	7689	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CNC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088889	7689	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CNC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2137	84	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1279	2137	84	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	4908365	2137	84	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL129198	2137	84	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	71525681	89699	0	None	2	6	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89699	0	None	2	6	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846300	80237	3	None	-2	4	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	80237	3	None	-2	4	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	156013896	178216	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	955	93	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	955	93	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	955	93	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	955	93	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	955	93	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	24994634	2129	31	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2129	31	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	11846300	80237	3	None	-2	4	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	80237	3	None	-2	4	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	71519674	89700	0	None	1	5	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89700	0	None	1	5	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	1204	1919	119	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	135398737	955	93	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	955	93	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	955	93	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	955	93	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	955	93	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	155513612	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156015638	178248	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1247	1919	119	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1375	1919	119	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	774	1919	119	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	CHEMBL90	1919	119	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	DB05381	1919	119	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	119570	3137	96	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	2233	3137	96	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	953	3137	96	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	CHEMBL301265	3137	96	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	DB00413	3137	96	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	168268716	192738	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5170276	192738	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221176	192738	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168268680	192742	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5172675	192742	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221210	192742	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	168268827	192755	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5179906	192755	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221314	192755	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	168268853	192761	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5180504	192761	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221353	192761	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	168273471	192777	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173079	192777	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221433	192777	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168273549	192779	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174342	192779	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221438	192779	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	168273640	192781	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5176229	192781	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221444	192781	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168273745	192784	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5178472	192784	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221451	192784	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	168274129	192785	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5176780	192785	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221468	192785	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168275750	192802	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5175558	192802	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221581	192802	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	168276867	192811	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174278	192811	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221653	192811	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	168277996	192822	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173373	192822	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221736	192822	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	168278471	192824	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183205	192824	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221757	192824	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	168278543	192827	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184846	192827	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221764	192827	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168280339	192841	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5188592	192841	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221885	192841	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168281536	192857	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5189006	192857	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221966	192857	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	168281746	192858	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184911	192858	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221974	192858	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	168283097	192869	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	CHEMBL5186770	192869	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222043	192869	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	168283321	192871	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183015	192871	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222057	192871	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	168283536	192876	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5187758	192876	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222074	192876	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	168284289	192882	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5194860	192882	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222123	192882	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168284480	192886	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5197548	192886	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222137	192886	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	168286141	192900	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5192993	192900	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222231	192900	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	168286426	192902	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5198795	192902	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222241	192902	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168290235	192944	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5201074	192944	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222491	192944	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168290815	192949	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5200771	192949	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222529	192949	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168291233	192955	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5200106	192955	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222556	192955	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	168292954	192973	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5202592	192973	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222660	192973	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	168294161	192982	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204739	192982	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222742	192982	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	168294445	192986	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208113	192986	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222768	192986	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	168294166	192990	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209081	192990	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222775	192990	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	168294449	192994	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204599	192994	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222793	192994	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	168294767	192995	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207281	192995	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222802	192995	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168294770	192996	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208327	192996	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222806	192996	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	168294776	193002	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209479	193002	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222831	193002	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	168295528	193007	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5206565	193007	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222872	193007	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168294178	193011	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5205197	193011	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222887	193011	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168295541	193016	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208845	193016	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222926	193016	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168295543	193017	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209021	193017	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222929	193017	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	168295803	193021	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207206	193021	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222937	193021	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168294182	193024	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208722	193024	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222960	193024	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	156013896	178216	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156015638	178248	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	178216	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	955	93	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	955	93	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	955	93	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	955	93	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	955	93	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	71525679	89697	0	None	6	6	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89697	0	None	6	6	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	155513612	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	11846300	80237	3	None	-2	4	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	80237	3	None	-2	4	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	24745335	294	12	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	294	12	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	294	12	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	294	12	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	1278	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	1279	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	4908365	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	1223	950	41	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	2790	950	41	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	CHEMBL14690	950	41	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	1223	950	41	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	2790	950	41	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	CHEMBL14690	950	41	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	156013896	178216	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	178216	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	56835171	69711	0	None	1	9	Human	7.4	pKd	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL1935574	69711	0	None	1	9	Human	7.4	pKd	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	155513612	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	178216	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	156013896	178216	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1919	119	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1919	119	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1919	119	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1919	119	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1919	119	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	156013896	178216	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156013896	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156013896	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	955	93	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	955	93	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	955	93	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	955	93	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	955	93	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	11846300	80237	3	None	-2	4	Human	7.1	pKd	=	7.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	80237	3	None	-2	4	Human	7.1	pKd	=	7.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	156015638	178248	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156015638	178248	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	71525678	89696	0	None	5	6	Human	8.1	pKd	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89696	0	None	5	6	Human	8.1	pKd	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	156013896	178216	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	54587788	61505	0	None	-	1	Human	10.4	pKi	=	10.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	4	3	5	1.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770975	61505	0	None	-	1	Human	10.4	pKi	=	10.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	4	3	5	1.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	23653543	68019	2	None	-	1	Human	10.0	pKi	=	10	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914541	68019	2	None	-	1	Human	10.0	pKi	=	10	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	54587787	61501	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770971	61501	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	46235428	6572	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6572	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	54586763	61498	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	3	2	5	1.9	CN1CCN(c2cc(N)nc(NCC(C)(C)C)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770968	61498	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	3	2	5	1.9	CN1CCN(c2cc(N)nc(NCC(C)(C)C)c2)CC1	10.1016/j.bmcl.2011.03.017
	24826169	68011	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914462	68011	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	16718567	8130	0	None	-	1	Human	9.4	pKi	=	9.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1091874	8130	0	None	-	1	Human	9.4	pKi	=	9.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737950	68102	0	None	-	1	Human	9.2	pKi	=	9.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	361	2	2	6	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914781	68102	0	None	-	1	Human	9.2	pKi	=	9.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	361	2	2	6	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	58227148	117107	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	117107	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	57399746	68077	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914755	68077	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	54580885	61503	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	4	3	5	1.8	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770973	61503	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	4	3	5	1.8	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	24826766	68072	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914750	68072	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	59777338	110101	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236556	110101	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	1204	1919	119	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1247	1919	119	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1375	1919	119	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	774	1919	119	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	CHEMBL90	1919	119	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	DB05381	1919	119	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1223	950	41	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	54581940	61522	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770992	61522	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	24797598	61532	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771001	61532	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	11224088	136432	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1021/jm800876b
	CHEMBL373579	136432	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1021/jm800876b
	24798144	110098	2	None	19	3	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3236553	110098	2	None	19	3	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	58227342	117094	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393534	117094	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	11224088	136432	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	CHEMBL373579	136432	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	24825998	68082	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914760	68082	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)CC1	10.1016/j.bmcl.2011.08.014
	57396301	68104	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	2.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914783	68104	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	2.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2011.08.014
	127050310	140810	0	None	-	1	Human	9.0	pKi	=	9	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	347	3	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(C(C)(F)F)cc23)C1	10.1039/C6MD00108D
	CHEMBL3819260	140810	0	None	-	1	Human	9.0	pKi	=	9	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	347	3	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(C(C)(F)F)cc23)C1	10.1039/C6MD00108D
	46222048	8952	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1098230	8952	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1016/j.bmc.2010.04.052
	24994542	110099	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236554	110099	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	24948752	68018	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.4	Nc1nc(N2CCCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914540	68018	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.4	Nc1nc(N2CCCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	24825727	68020	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914542	68020	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	46222048	8952	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Inhibition of human recombinant histamine H4 receptor expressed in CHO cellsInhibition of human recombinant histamine H4 receptor expressed in CHO cells	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1021/jm901890s
	CHEMBL1098230	8952	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Inhibition of human recombinant histamine H4 receptor expressed in CHO cellsInhibition of human recombinant histamine H4 receptor expressed in CHO cells	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1021/jm901890s
	155524115	176523	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	176523	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	176523	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	24798144	110098	2	None	19	3	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	110098	2	None	19	3	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	155524115	176523	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	176523	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	176523	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	24797604	110097	2	None	-	1	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	CHEMBL3236552	110097	2	None	-	1	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	44231618	5858	0	None	1	4	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1079516	5858	0	None	1	4	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	25128824	193154	0	None	3	2	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	193154	0	None	3	2	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	24992077	110116	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236571	110116	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	44231618	5858	0	None	-1	4	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1079516	5858	0	None	-1	4	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	44129130	199533	0	None	2	2	Rat	8.8	pKi	=	8.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	199533	0	None	2	2	Rat	8.8	pKi	=	8.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1223	950	41	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	44187557	195476	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202332	195476	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL553423	195476	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1919	119	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	1247	1919	119	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	1375	1919	119	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	774	1919	119	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	CHEMBL90	1919	119	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	DB05381	1919	119	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	11957573	1997	34	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	1250	1997	34	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	3692	1997	34	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	CHEMBL19439	1997	34	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	16202258	68189	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915536	68189	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	66740859	110124	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	327	3	2	5	2.3	CN[C@@H]1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236579	110124	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	327	3	2	5	2.3	CN[C@@H]1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)C1	10.1021/jm401727m
	24798232	110105	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	1.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236560	110105	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	1.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCCN[C@H]3C2)n1	10.1021/jm401727m
	44231735	7753	0	None	1	4	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089390	7753	0	None	1	4	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	25129527	187390	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	187390	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	135525668	68183	0	None	263	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915348	68183	0	None	263	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	25217643	112143	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysis	ChEMBL	289	2	0	5	1.9	CN1CCN(c2ncnc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290570	112143	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysis	ChEMBL	289	2	0	5	1.9	CN1CCN(c2ncnc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1249	1996	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	13499360	1996	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90019	1996	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10176414	4032	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1246	4032	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL281576	4032	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10060212	204511	0	None	5	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72769	204511	0	None	5	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681807	89617	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	8	3	8	1.6	CC(C)CCc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375668	89617	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	8	3	8	1.6	CC(C)CCc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681804	89642	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1ccnc(N)n1)c1cc(-c2ccccc2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375770	89642	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1ccnc(N)n1)c1cc(-c2ccccc2)nc(N)n1	10.1021/jm301886t
	1223	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1223	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1249	1996	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	13499360	1996	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	CHEMBL90019	1996	16	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	53233802	61517	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770987	61517	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	70692529	75654	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	303	2	1	2	2.7	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047466	75654	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	303	2	1	2	2.7	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	46228994	199406	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	466	6	1	7	2.7	CC1CCCCN1S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591249	199406	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	466	6	1	7	2.7	CC1CCCCN1S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	1223	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	2790	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	CHEMBL14690	950	41	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	45268092	195991	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1743881	195991	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560111	195991	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45268093	195992	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202321	195992	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560112	195992	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45267230	196459	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202318	196459	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL563342	196459	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	24737954	68100	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(F)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914778	68100	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(F)c23)C1	10.1016/j.bmcl.2011.08.014
	24826522	68107	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914786	68107	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	24826168	68110	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914789	68110	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24745335	294	12	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	294	12	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	294	12	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	294	12	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	71525680	89702	0	None	97	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	284	3	2	6	1.3	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376806	89702	0	None	97	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	284	3	2	6	1.3	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	71525678	89696	0	None	5	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89696	0	None	5	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25101945	193082	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	193082	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	136192886	56450	0	None	-1	4	Mouse	7.0	pKi	=	7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56450	0	None	-1	4	Mouse	7.0	pKi	=	7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	44129134	199485	0	None	-22	2	Rat	7.0	pKi	=	7	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	199485	0	None	-22	2	Rat	7.0	pKi	=	7	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11694543	75770	4	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	6	2	2	1.3	CCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204843	75770	4	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	6	2	2	1.3	CCNCCCCc1c[nH]cn1	10.1021/jm0504353
	11521414	141272	0	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	2	2	2.7	c1nc(CCCCNC2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL383324	141272	0	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	2	2	2.7	c1nc(CCCCNC2CCCCC2)c[nH]1	10.1021/jm0504353
	9812843	205221	1	None	-4	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1016/j.bmcl.2011.06.123
	CHEMBL78838	205221	1	None	-4	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1016/j.bmcl.2011.06.123
	53233904	61495	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770965	61495	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	39732699	75651	2	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	1	2	2	1.4	O=C(c1cc2cccc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047463	75651	2	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	1	2	2	1.4	O=C(c1cc2cccc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	70696623	75659	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	2	2	2	2.0	CNC1CN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047471	75659	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	2	2	2	2.0	CNC1CN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	51354298	58857	2	None	1	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	233	4	1	4	1.9	CC(C)(C)c1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688944	58857	2	None	1	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	233	4	1	4	1.9	CC(C)(C)c1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	45267237	196179	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202320	196179	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL561451	196179	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	53378933	110118	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	269	3	2	5	1.2	Nc1nc(Cc2ccccc2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236573	110118	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	269	3	2	5	1.2	Nc1nc(Cc2ccccc2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	11087453	75615	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL2047270	75615	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1039/C2MD20212C
	44395335	67032	0	None	25	2	Human	6.0	pKi	=	6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	266	4	2	3	1.5	CN(C)CCNC(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL187210	67032	0	None	25	2	Human	6.0	pKi	=	6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	266	4	2	3	1.5	CN(C)CCNC(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	10978141	5118	1	None	-4	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL105803	5118	1	None	-4	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	71681477	89629	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	398	7	4	8	3.3	Nc1nc(NCCNc2cc(-c3ccccc3)nc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375758	89629	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	398	7	4	8	3.3	Nc1nc(NCCNc2cc(-c3ccccc3)nc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	71681802	89640	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1cc(NCCNc2ccnc(N)n2)ncn1	10.1021/jm301886t
	CHEMBL2375769	89640	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1cc(NCCNc2ccnc(N)n2)ncn1	10.1021/jm301886t
	206876	18913	7	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL128637	18913	7	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	66572107	75610	1	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047265	75610	1	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	66572115	75630	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047442	75630	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	11469514	104141	2	None	-64	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL309654	104141	2	None	-64	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	46890591	6993	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	436	6	1	4	4.8	Cc1cc(OCCCN2CC3CCCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084811	6993	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	436	6	1	4	4.8	Cc1cc(OCCCN2CC3CCCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44275807	98942	0	None	-9120	10	Human	5.0	pKi	=	5	Binding	Binding affinity towards human histaminergic (H4) receptorBinding affinity towards human histaminergic (H4) receptor	ChEMBL	422	7	0	4	4.5	C[C@H]1CC[C@H](C)N1CCCOc1ccc(-c2ccc(C(=O)N3CCOCC3)cc2)cc1	10.1016/s0960-894x(03)00118-5
	CHEMBL27979	98942	0	None	-9120	10	Human	5.0	pKi	=	5	Binding	Binding affinity towards human histaminergic (H4) receptorBinding affinity towards human histaminergic (H4) receptor	ChEMBL	422	7	0	4	4.5	C[C@H]1CC[C@H](C)N1CCCOc1ccc(-c2ccc(C(=O)N3CCOCC3)cc2)cc1	10.1016/s0960-894x(03)00118-5
	4242418	117406	12	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	2	1	2	1.9	CN1CCN(Cc2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL339826	117406	12	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	2	1	2	1.9	CN1CCN(Cc2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	71681978	89624	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	310	5	3	5	1.7	CN(CCNc1ccnc(N)n1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm301886t
	CHEMBL2375753	89624	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	310	5	3	5	1.7	CN(CCNc1ccnc(N)n1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm301886t
	58227158	117109	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1cc(N2CCN(C)CC2)cnn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393549	117109	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1cc(N2CCN(C)CC2)cnn1	10.1016/j.bmcl.2014.12.027
	57391926	69873	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938967	69873	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11846300	80237	3	None	-2	4	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL213912	80237	3	None	-2	4	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2015.12.035
	24854583	96093	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	304	2	0	4	3.0	CN1CCN(c2nc3ccccc3nc2-c2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260372	96093	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	304	2	0	4	3.0	CN1CCN(c2nc3ccccc3nc2-c2ccccc2)CC1	10.1021/jm7014217
	16082860	138586	0	None	-7	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL377572	138586	0	None	-7	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	57391032	68071	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@H]3C[C@@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914749	68071	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@H]3C[C@@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25179297	173807	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL453877	173807	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	24759518	179285	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL473302	179285	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178342	186762	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489070	186762	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179297	173807	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL453877	173807	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25178342	186762	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489070	186762	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	71518535	86458	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	1	5	2.6	CN1CCN(c2nc(N(C)C)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314763	86458	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	1	5	2.6	CN1CCN(c2nc(N(C)C)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	10978141	5118	1	None	-4	2	Human	6.0	pKi	=	6.0	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL105803	5118	1	None	-4	2	Human	6.0	pKi	=	6.0	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	57393730	67855	0	None	-2754	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	579	8	2	6	5.0	O=C(c1cc2ccccc2[nH]1)N1CCN(CCN2CCC(Nc3nc4ccccc4n3Cc3ccc(F)cc3)CC2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910386	67855	0	None	-2754	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	579	8	2	6	5.0	O=C(c1cc2ccccc2[nH]1)N1CCN(CCN2CCC(Nc3nc4ccccc4n3Cc3ccc(F)cc3)CC2)CC1	10.1016/j.bmcl.2011.09.001
	24826681	68090	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914768	68090	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	127049855	140203	0	None	-1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	140203	0	None	-1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	57391869	63090	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774589	63090	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789349	63090	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	90645163	112163	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1cc(C)cc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290590	112163	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1cc(C)cc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	10656971	16389	4	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL123137	16389	4	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1016/j.bmcl.2011.07.114
	121238070	166463	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	4	1	7	1.4	CN1CCN(c2nc(N)nc(COc3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4278404	166463	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	4	1	7	1.4	CN1CCN(c2nc(N)nc(COc3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178555	174215	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454891	174215	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178555	174215	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454891	174215	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	155540201	172884	0	None	-67	5	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	513	16	2	4	5.5	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc(Cl)cc1	10.1039/C8MD00527C
	CHEMBL4516173	172884	0	None	-67	5	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	513	16	2	4	5.5	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc(Cl)cc1	10.1039/C8MD00527C
	1278	2137	84	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	832283	12366	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL118572	12366	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	832283	12366	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL118572	12366	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	10269628	138691	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	355	1	0	4	4.6	CC(C)N1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL377985	138691	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	355	1	0	4	4.6	CC(C)N1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	54582866	61518	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(N)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770988	61518	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(N)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2011.03.017
	25133970	187564	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL494679	187564	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	49841962	69699	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3c(-c4ccoc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935562	69699	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3c(-c4ccoc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	44547568	117090	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393530	117090	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	13731320	158626	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	2	2	2	4.0	O=C(Nc1cccc2ccccc12)N1CCC(c2c[nH]cn2)CC1	10.1016/j.ejmech.2016.09.074
	CHEMBL4092578	158626	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	2	2	2	4.0	O=C(Nc1cccc2ccccc12)N1CCC(c2c[nH]cn2)CC1	10.1016/j.ejmech.2016.09.074
	127035837	137392	0	None	-5	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	403	4	1	5	3.7	CN1CCN(c2nc(NCc3ccc(F)cc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753334	137392	0	None	-5	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	403	4	1	5	3.7	CN1CCN(c2nc(NCc3ccc(F)cc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	21842421	112144	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	270	2	1	6	0.9	CN1CCN(c2nc(N)nc(-c3ccccc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290571	112144	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	270	2	1	6	0.9	CN1CCN(c2nc(N)nc(-c3ccccc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10688021	156087	0	None	-1	2	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	332	8	1	3	4.3	O=C(/C=C/c1ccccc1)c1ccc(OCCCc2c[nH]cn2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4063170	156087	0	None	-1	2	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	332	8	1	3	4.3	O=C(/C=C/c1ccccc1)c1ccc(OCCCc2c[nH]cn2)cc1	10.1016/j.ejmech.2016.09.074
	9944571	116991	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL338937	116991	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9944571	116991	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338937	116991	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	23650961	3817	26	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	9276	3817	26	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	CHEMBL3301609	3817	26	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	DB12522	3817	26	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	24803710	150305	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	400	6	2	5	4.1	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(F)c(F)ccc2[nH]1	nan
	CHEMBL3953011	150305	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	400	6	2	5	4.1	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(F)c(F)ccc2[nH]1	nan
	25101945	193082	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	193082	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	118720437	115887	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	275	3	2	5	1.0	CN[C@@H]1CCN(c2cc(CC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354811	115887	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	275	3	2	5	1.0	CN[C@@H]1CCN(c2cc(CC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	24737952	68105	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(OC)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914784	68105	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(OC)cc23)C1	10.1016/j.bmcl.2011.08.014
	9944571	116991	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338937	116991	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	24996854	110109	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	CHEMBL3236564	110109	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	71603142	89692	0	None	54	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	3	2	5	1.2	CCc1ccc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376796	89692	0	None	54	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	3	2	5	1.2	CCc1ccc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	44129282	199176	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)cc(Cl)c3n2)CC1	10.1021/jm901379s
	CHEMBL589746	199176	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)cc(Cl)c3n2)CC1	10.1021/jm901379s
	71603139	89689	0	None	13	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	2	2	5	1.5	Nc1nc(N2CCNCC2)c2ccc(C3CC3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376793	89689	0	None	13	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	2	2	5	1.5	Nc1nc(N2CCNCC2)c2ccc(C3CC3)cc2n1	10.1016/j.bmcl.2013.02.091
	45257132	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	155513612	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	44450493	96505	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	1	1	4	1.3	CN1CCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL262859	96505	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	1	1	4	1.3	CN1CCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	1278	2137	84	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	126963283	160939	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL4115677	160939	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	1224	1420	83	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	3100	1420	83	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	8980	1420	83	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	916	1420	83	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	CHEMBL657	1420	83	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	DB01075	1420	83	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	1241	2024	52	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	1242	2024	52	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	3614	2024	52	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	CHEMBL507	2024	52	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	49841609	69693	15	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3c(Br)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935556	69693	15	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3c(Br)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	51354871	58875	0	None	-22	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	290	6	0	4	3.3	c1c(CC2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1688982	58875	0	None	-22	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	290	6	0	4	3.3	c1c(CC2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	164618508	185908	0	None	-363	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	6	4	4	2.7	Cc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4869194	185908	0	None	-363	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	6	4	4	2.7	Cc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	164620678	185995	0	None	-177	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.7	CCC(NC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4870510	185995	0	None	-177	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.7	CCC(NC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	58227159	176361	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4534254	176361	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595907	176361	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	122193099	123892	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	374	3	1	6	2.1	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Br)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628046	123892	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	374	3	1	6	2.1	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Br)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	51354008	58852	0	None	-3	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	253	5	1	4	1.8	c1ccc(Cc2cn(CCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688939	58852	0	None	-3	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	253	5	1	4	1.8	c1ccc(Cc2cn(CCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	51354586	58872	0	None	-15	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	2.9	CC(c1cn(CCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	CHEMBL1688978	58872	0	None	-15	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	2.9	CC(c1cn(CCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	16082824	77919	0	None	1	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL209598	77919	0	None	1	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	54585778	61519	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(N)nc(NCCO)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770989	61519	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(N)nc(NCCO)n2)CC1	10.1016/j.bmcl.2011.03.017
	58227165	117096	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393536	117096	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	24853664	96312	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261517	96312	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	10773349	8059	1	None	-11	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL109130	8059	1	None	-11	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10715857	74987	1	None	-3	3	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	398	8	1	3	3.5	O=C(CCCc1ccc(I)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL20338	74987	1	None	-3	3	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	398	8	1	3	3.5	O=C(CCCc1ccc(I)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	16082820	80089	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213250	80089	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	10776393	5464	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1076267	5464	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL156912	5464	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	118725542	117097	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393537	117097	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	1278	2137	84	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2137	84	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2137	84	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2137	84	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	90645161	112161	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3cc(F)cc(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290588	112161	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3cc(F)cc(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	45257132	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	24994634	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	24994634	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	8984	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	24994635	110115	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236570	110115	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	24826442	68078	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1ccc2oc3c(N4CCN(C)CC4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914756	68078	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1ccc2oc3c(N4CCN(C)CC4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	155513612	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	1226	2006	20	None	2	7	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	41376	2006	20	None	2	7	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	CHEMBL12608	2006	20	None	2	7	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	24745335	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	71603135	89685	0	None	26	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	2	2	5	2.3	Nc1nc(N2CCNCC2)c2ccc(C3CCCC3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376789	89685	0	None	26	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	2	2	5	2.3	Nc1nc(N2CCNCC2)c2ccc(C3CCCC3)cc2n1	10.1016/j.bmcl.2013.02.091
	71525589	89703	0	None	11	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	286	3	2	6	1.5	CN[C@@H]1CCN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376807	89703	0	None	11	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	286	3	2	6	1.5	CN[C@@H]1CCN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	24745335	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	294	12	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	24994634	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	24994634	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	3035746	188156	9	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL1237146	188156	9	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL498770	188156	9	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	155513612	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	176296	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1249	1996	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	13499360	1996	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90019	1996	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1253	2045	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	132937	2045	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19010	2045	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10023327	168992	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	10023327	168992	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	10023327	168992	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	1253	2045	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	132937	2045	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL19010	2045	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	11065080	19402	2	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL129488	19402	2	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	66573460	75639	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.2	Cc1cc(Cl)cc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047451	75639	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.2	Cc1cc(Cl)cc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	21638785	188634	5	None	-4	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL503605	188634	5	None	-4	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	44129132	199511	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	6	1	7	1.3	CN1CCN(c2nc(NCCS(=O)(=O)N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592188	199511	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	6	1	7	1.3	CN1CCN(c2nc(NCCS(=O)(=O)N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10176414	4032	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	1246	4032	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL281576	4032	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	45267233	194884	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202319	194884	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL541171	194884	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1249	1996	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	13499360	1996	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90019	1996	16	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	25128823	192693	0	None	-2	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192693	0	None	-2	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	127050431	140290	0	None	4	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	140290	0	None	4	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11451949	68016	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	384	6	1	4	3.9	CN1CCN(CCCOc2ccc(-c3nc4cc(Cl)ccc4[nH]3)cc2)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914533	68016	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	384	6	1	4	3.9	CN1CCN(CCCOc2ccc(-c3nc4cc(Cl)ccc4[nH]3)cc2)CC1	10.1016/j.bmcl.2011.08.014
	46228126	202589	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.4	CN1CCN(c2cc(NCc3ccc(C(F)(F)F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL612131	202589	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.4	CN1CCN(c2cc(NCc3ccc(C(F)(F)F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	60150166	174700	0	None	-323	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	524	17	2	6	4.7	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc([N+](=O)[O-])cc1	10.1039/C8MD00527C
	CHEMBL4560123	174700	0	None	-323	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	524	17	2	6	4.7	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc([N+](=O)[O-])cc1	10.1039/C8MD00527C
	168295543	193017	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209021	193017	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222929	193017	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	164610648	184814	0	None	-120	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4852212	184814	0	None	-120	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	10820930	5767	0	None	1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL107877	5767	0	None	1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	24826443	68092	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914770	68092	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	10800875	6248	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1081718	6248	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL345828	6248	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	24745335	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	294	12	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	1223	950	41	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	10800875	6248	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1081718	6248	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL345828	6248	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	11206058	104388	9	None	-10	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL310360	104388	9	None	-10	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	1267	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3035905	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	CHEMBL260374	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3062060	10349	5	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	221	4	6	3	-1.0	N=C(N)NCc1cccc(CNC(=N)N)n1	10.1021/jm7014777
	CHEMBL1162346	10349	5	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	221	4	6	3	-1.0	N=C(N)NCc1cccc(CNC(=N)N)n1	10.1021/jm7014777
	11511026	16004	0	None	-208	4	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3ccc4ccccc4n3)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	CHEMBL1223683	16004	0	None	-208	4	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3ccc4ccccc4n3)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	1267	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	3035905	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	CHEMBL260374	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	10106063	109244	10	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting method	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	CHEMBL321860	109244	10	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting method	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	24826000	68114	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	2	2	6	3.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914793	68114	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	2	2	6	3.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)C1	10.1016/j.bmcl.2011.08.014
	1267	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	3035905	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	CHEMBL260374	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	1278	2137	84	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10106063	109244	10	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321860	109244	10	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	9974306	204451	0	None	-15	4	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72372	204451	0	None	-15	4	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	56849569	71583	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923033	71583	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963332	71583	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11499453	76182	0	None	-79	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	2	2	2.4	CC(C)CNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205834	76182	0	None	-79	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	2	2	2.4	CC(C)CNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11672850	76644	3	None	-10	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.1	CC(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206451	76644	3	None	-10	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.1	CC(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	1267	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	3035905	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	CHEMBL260374	3775	49	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	10106063	109244	10	None	16	2	Human	6.9	pKi	=	6.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321860	109244	10	None	16	2	Human	6.9	pKi	=	6.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	5310982	93583	4	None	-1348	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	CHEMBL24665	93583	4	None	-1348	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	71681975	89621	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	296	5	4	5	1.4	Nc1nccc(NCCNC(=O)c2cc3ccccc3[nH]2)n1	10.1021/jm301886t
	CHEMBL2375750	89621	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	296	5	4	5	1.4	Nc1nccc(NCCNC(=O)c2cc3ccccc3[nH]2)n1	10.1021/jm301886t
	71681642	89636	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nc(NCCNc2ccnc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375765	89636	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nc(NCCNc2ccnc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	11286909	205261	2	None	-1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL79126	205261	2	None	-1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	10678661	5330	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106931	5330	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	11043445	56449	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632410	56449	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	168294449	192994	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204599	192994	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222793	192994	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	66572111	75616	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047271	75616	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	118720435	115886	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	225	3	2	5	0.3	CN[C@@H]1CCN(c2cc(CF)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354809	115886	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	225	3	2	5	0.3	CN[C@@H]1CCN(c2cc(CF)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	90645151	112145	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290572	112145	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2014.06.032
	16082833	80318	0	None	-23	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214312	80318	0	None	-23	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	54584826	61523	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770993	61523	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	57395538	68182	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915347	68182	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	57395538	68182	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915347	68182	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	25178343	172567	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL448962	172567	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178343	172567	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL448962	172567	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	57403269	68088	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	1	6	2.5	CN1CCN(c2nc(N)nc3c4cc(Cl)ccc4[s+]([O-])c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914766	68088	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	1	6	2.5	CN1CCN(c2nc(N)nc3c4cc(Cl)ccc4[s+]([O-])c23)CC1	10.1016/j.bmcl.2011.08.014
	24854705	96082	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	3	0	6	2.6	CN1CCN(c2nc3ccccc3nc2Oc2cccnc2)CC1	10.1021/jm7014217
	CHEMBL260274	96082	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	3	0	6	2.6	CN1CCN(c2nc3ccccc3nc2Oc2cccnc2)CC1	10.1021/jm7014217
	164619208	185638	0	None	-446	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccccc1F	10.1016/j.ejmech.2021.113190
	CHEMBL4864852	185638	0	None	-446	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccccc1F	10.1016/j.ejmech.2021.113190
	10678661	5330	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106931	5330	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	57402929	69838	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938844	69838	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	57392827	68094	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914772	68094	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	58227163	176439	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	176439	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	176439	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	1204	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	1247	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	1375	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	774	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	DB05381	1919	119	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	16082856	81084	0	None	-2	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL215628	81084	0	None	-2	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	46929647	16726	0	None	-91	3	Human	5.9	pKi	=	5.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	CHEMBL1243394	16726	0	None	-91	3	Human	5.9	pKi	=	5.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	11858011	158979	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	9	0	3	4.6	O=C(c1ccccc1)c1ccc(OCCCCCN2CCCC2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4096444	158979	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	9	0	3	4.6	O=C(c1ccccc1)c1ccc(OCCCCCN2CCCC2)cc1	10.1016/j.ejmech.2016.09.074
	16352	117159	13	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL339414	117159	13	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	11240759	105186	5	None	-190	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL311473	105186	5	None	-190	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	24853662	96046	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	3	0	5	3.1	CN1CCN(c2nc3ccccc3nc2OC2CCCCC2)CC1	10.1021/jm7014217
	CHEMBL260043	96046	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	3	0	5	3.1	CN1CCN(c2nc3ccccc3nc2OC2CCCCC2)CC1	10.1021/jm7014217
	54584378	63129	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774595	63129	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789782	63129	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164626153	186354	0	None	-6456	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccc(F)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4875600	186354	0	None	-6456	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccc(F)c1	10.1016/j.ejmech.2021.113190
	54583874	61516	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N)nc(N3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770986	61516	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N)nc(N3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	54584378	63129	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774595	63129	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789782	63129	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	46890122	7341	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	421	9	2	5	4.3	Cc1cc(OCCCNCCc2cn(C)cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086344	7341	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	421	9	2	5	4.3	Cc1cc(OCCCNCCc2cn(C)cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	155568016	176014	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	4	1	6	1.1	CCC(CC)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4590367	176014	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	4	1	6	1.1	CCC(CC)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	58227319	117100	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393540	117100	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	56849571	71584	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923034	71584	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963347	71584	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	25179126	173389	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm800876b
	CHEMBL452849	173389	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm800876b
	25179126	173389	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm901379s
	CHEMBL452849	173389	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm901379s
	164609729	185074	0	None	-2187	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856059	185074	0	None	-2187	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	164622886	185918	0	None	-131	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4869389	185918	0	None	-131	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	164626509	186311	0	None	-3	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	358	3	4	4	3.2	N/C(=N\C(=O)NC1CCCCC1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4874969	186311	0	None	-3	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	358	3	4	4	3.2	N/C(=N\C(=O)NC1CCCCC1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	11043445	56449	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL1632410	56449	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	1204	1919	119	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1919	119	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1919	119	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1919	119	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1919	119	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1919	119	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	11609325	14498	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.3	CN1CCN(C(=O)c2nc3c(Cl)cc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL120221	14498	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.3	CN1CCN(C(=O)c2nc3c(Cl)cc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	24825796	68066	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	NC[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914744	68066	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	NC[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	46228100	199993	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	315	4	1	6	2.1	CN1CCN(c2cc(SCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595413	199993	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	315	4	1	6	2.1	CN1CCN(c2cc(SCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	44628631	68095	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3c(Cl)cc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914773	68095	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3c(Cl)cc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	71525679	89697	0	None	-9	6	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89697	0	None	-9	6	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	4060730	19347	56	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL129199	19347	56	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	24853594	96108	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	4	0	5	3.3	Cc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL260470	96108	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	4	0	5	3.3	Cc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	44414495	79990	0	None	-34	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	5	0	2	6.3	CC1CC(C)CN(Cc2ccc(-c3ccc(CN4CC(C)CC(C)C4)cc3)cc2)C1	10.1016/j.bmc.2008.10.029
	CHEMBL212869	79990	0	None	-34	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	5	0	2	6.3	CC1CC(C)CN(Cc2ccc(-c3ccc(CN4CC(C)CC(C)C4)cc3)cc2)C1	10.1016/j.bmc.2008.10.029
	127026017	137333	0	None	-12	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752972	137333	0	None	-12	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)cc1	10.1016/j.bmcl.2015.12.035
	164614907	184560	0	None	-436	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(F)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848697	184560	0	None	-436	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(F)cc1	10.1016/j.ejmech.2021.113190
	57390263	68158	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	330	1	2	7	1.8	Nc1nc(N2CC3CCNC3C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915052	68158	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	330	1	2	7	1.8	Nc1nc(N2CC3CCNC3C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	9900313	19392	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129444	19392	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9900313	19392	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129444	19392	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0341047
	1278	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2137	84	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	46890592	6360	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6360	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	9900313	19392	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129444	19392	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	156013896	178216	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	178216	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	178216	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25129523	187263	45	None	-1	2	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	187263	45	None	-1	2	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	1249	1996	16	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	13499360	1996	16	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	CHEMBL90019	1996	16	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	1204	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1247	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1375	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	774	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	DB05381	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	137655927	158864	0	None	17	2	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	403	7	2	3	5.2	O=C(NC(c1ccc(Cl)cc1)c1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL4095224	158864	0	None	17	2	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	403	7	2	3	5.2	O=C(NC(c1ccc(Cl)cc1)c1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	71603141	89691	0	None	14	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	5	1.2	CCc1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376795	89691	0	None	14	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	5	1.2	CCc1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	54585779	61521	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770991	61521	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25179303	189322	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCCC3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL512568	189322	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCCC3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	51354304	58866	0	None	-3	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCn2cc(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688972	58866	0	None	-3	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCn2cc(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	71519016	86440	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314501	86440	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	71518859	86466	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	294	2	3	5	1.6	Nc1nc(N2CCNCC2)c2cc(-c3ccccc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314772	86466	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	294	2	3	5	1.6	Nc1nc(N2CCNCC2)c2cc(-c3ccccc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	156014317	178253	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634728	178253	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650854	178253	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	11724853	96991	0	None	-19	4	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	340	0	0	4	3.7	CN1CCN(C2=Nc3cc(Cl)ccc3N(C)c3ccccc32)CC1	10.1021/jm051008s
	CHEMBL266723	96991	0	None	-19	4	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	340	0	0	4	3.7	CN1CCN(C2=Nc3cc(Cl)ccc3N(C)c3ccccc32)CC1	10.1021/jm051008s
	57398985	67854	0	None	-1122	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	569	12	2	7	4.4	COc1ccc(CN(CCN(C)CC(C)Nc2ccc3[nH]c(C(=O)N4CCN(C)CC4)cc3c2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910385	67854	0	None	-1122	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	569	12	2	7	4.4	COc1ccc(CN(CCN(C)CC(C)Nc2ccc3[nH]c(C(=O)N4CCN(C)CC4)cc3c2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	118725539	117083	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	2	0	4	1.8	CN1CCN(c2ccnc(N3CCCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393523	117083	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	2	0	4	1.8	CN1CCN(c2ccnc(N3CCCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	16082822	79951	0	None	-4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@@H]2C[C@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL212705	79951	0	None	-4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@@H]2C[C@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	46228160	199836	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	308	3	0	6	1.6	CN1CCN(c2ncnc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594468	199836	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	308	3	0	6	1.6	CN1CCN(c2ncnc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	2306318	86979	12	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1039/C5MD00022J
	CHEMBL232421	86979	12	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1039/C5MD00022J
	127036980	137321	0	None	-16	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752885	137321	0	None	-16	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	57391033	68091	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	287	1	2	6	1.5	Nc1nc(N2CCNCC2)c2oc3cccc(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914769	68091	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	287	1	2	6	1.5	Nc1nc(N2CCNCC2)c2oc3cccc(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	1236	2054	25	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	1237	2054	25	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	156615	2054	25	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	CHEMBL268229	2054	25	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	24797248	199843	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594490	199843	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	9951970	5468	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1076300	5468	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL154797	5468	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	121238066	166668	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	2	8	-1.0	CN1CCN(c2nc(N)nc(CN3C(=O)NC(C)(C)C3=O)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4282283	166668	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	2	8	-1.0	CN1CCN(c2nc(N)nc(CN3C(=O)NC(C)(C)C3=O)n2)CC1	10.1016/j.ejmech.2017.09.035
	10860228	116809	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338001	116809	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0341047
	10860228	116809	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338001	116809	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0502081
	3840250	136592	16	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	5	2.4	Cc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	CHEMBL3739776	136592	16	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	5	2.4	Cc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	56851447	71596	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1923026	71596	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1963433	71596	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	90645157	112157	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1Cl	10.1016/j.ejmech.2014.06.032
	CHEMBL3290584	112157	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1Cl	10.1016/j.ejmech.2014.06.032
	2971762	193203	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL523617	193203	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	44450401	156107	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	3	2	4	2.0	O=c1[nH]c2ccc(Cl)cc2nc1NCN1CCCC1	10.1021/jm7014217
	CHEMBL406345	156107	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	3	2	4	2.0	O=c1[nH]c2ccc(Cl)cc2nc1NCN1CCCC1	10.1021/jm7014217
	2971762	193203	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL523617	193203	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	164622190	185592	0	None	-93	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4864067	185592	0	None	-93	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCCc1ccccc1	10.1016/j.ejmech.2021.113190
	54586762	61497	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	4	2	5	2.0	CN1CCN(c2cc(N)nc(NCc3ccccc3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770967	61497	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	4	2	5	2.0	CN1CCN(c2cc(N)nc(NCc3ccccc3)c2)CC1	10.1016/j.bmcl.2011.03.017
	136066109	200411	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	46231557	200411	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	CHEMBL598291	200411	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	5477514	10348	1	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	408	4	4	5	2.6	C/N=C(\N)N/N=C(/C)c1ccc2sc3ccc(/C(C)=N\N/C(N)=N/C)cc3c2c1	10.1021/jm7014777
	CHEMBL1162345	10348	1	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	408	4	4	5	2.6	C/N=C(\N)N/N=C(/C)c1ccc2sc3ccc(/C(C)=N\N/C(N)=N/C)cc3c2c1	10.1021/jm7014777
	24826083	68118	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914797	68118	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	10988959	118082	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL340740	118082	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	4906599	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL436381	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10988959	118082	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL340740	118082	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0341047
	4906599	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL436381	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0341047
	57391819	69707	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935570	69707	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)c1	10.1016/j.bmcl.2011.10.104
	53233801	61515	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770985	61515	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	10988959	118082	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL340740	118082	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	4906599	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL436381	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	71519674	89700	0	None	-1	5	Mouse	7.8	pKi	=	7.8	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89700	0	None	-1	5	Mouse	7.8	pKi	=	7.8	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	56835171	69711	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL1935574	69711	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	71603138	89688	0	None	32	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	2	5	1.9	CNC1CN(c2nc(N)nc3cc(C(C)(C)C)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376792	89688	0	None	32	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	2	5	1.9	CNC1CN(c2nc(N)nc3cc(C(C)(C)C)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25128822	187457	0	None	-10	5	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	187457	0	None	-10	5	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	44129214	199178	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	538	7	2	7	3.7	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Br)c(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL589782	199178	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	538	7	2	7	3.7	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Br)c(Cl)cc3n2)CC1	10.1021/jm901379s
	136192874	56453	0	None	-1	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56453	0	None	-1	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25147772	173385	9	None	1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	173385	9	None	1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	136192874	56453	0	None	-1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56453	0	None	-1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1247	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1375	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	774	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	CHEMBL90	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	DB05381	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1223	950	41	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1204	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1247	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1375	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	774	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL90	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	DB05381	1919	119	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1278	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	1279	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	4908365	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	CHEMBL129198	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	10967174	19410	3	None	-2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129540	19410	3	None	-2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70681963	75658	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	2	1	2	2.9	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047470	75658	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	2	1	2	2.9	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	4906599	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL436381	168638	11	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1278	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	1279	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	4908365	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	CHEMBL129198	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	3035746	188156	9	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL1237146	188156	9	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL498770	188156	9	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	45269820	195326	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202326	195326	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL551790	195326	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1278	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	1279	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	4908365	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	CHEMBL129198	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	155491015	176471	4	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	176471	4	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	176471	4	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	25129525	187559	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1039/C2MD20212C
	CHEMBL494648	187559	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1039/C2MD20212C
	46228125	199965	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595215	199965	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890020	7336	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	8	3	4	4.5	Cc1cc(OCCCNCC2CCCCN2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086327	7336	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	8	3	4	4.5	Cc1cc(OCCCNCC2CCCCN2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	42641407	199363	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(Cl)cc(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL591013	199363	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(Cl)cc(Cl)cc3n2)CC1	10.1021/jm901379s
	127036981	137425	0	None	-1	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753621	137425	0	None	-1	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	135570464	179073	3	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	1	5	1.7	CN1CCN(c2nc(O)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL471712	179073	3	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	1	5	1.7	CN1CCN(c2nc(O)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354297	58856	0	None	-5	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	289	6	1	6	0.4	CN1CCN(Cc2cn(CCCc3c[nH]cn3)nn2)CC1	10.1021/jm1013488
	CHEMBL1688943	58856	0	None	-5	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	289	6	1	6	0.4	CN1CCN(Cc2cn(CCCc3c[nH]cn3)nn2)CC1	10.1021/jm1013488
	46890074	6571	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	403	8	1	3	6.2	Cc1cc(OCCCCCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083160	6571	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	403	8	1	3	6.2	Cc1cc(OCCCCCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	10107667	63135	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774599	63135	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789800	63135	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	25178755	176850	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCC[C@H]3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL461537	176850	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCC[C@H]3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	24759518	179285	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL473302	179285	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1267	3775	49	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	118720439	115893	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	246	2	2	4	2.2	Nc1nc(C2CCCC2)cc(C2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354817	115893	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	246	2	2	4	2.2	Nc1nc(C2CCCC2)cc(C2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	24853527	167403	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	4	0	6	3.2	CN1CCN(c2nc3ccccc3nc2Oc2cccc(N(C)C)c2)CC1	10.1021/jm7014217
	CHEMBL429691	167403	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	4	0	6	3.2	CN1CCN(c2nc3ccccc3nc2Oc2cccc(N(C)C)c2)CC1	10.1021/jm7014217
	10107667	63135	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774599	63135	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789800	63135	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	10760516	63142	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774598	63142	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789829	63142	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	127050431	140290	0	None	4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	140290	0	None	4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	10760516	63142	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774598	63142	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789829	63142	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	57391034	68115	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914794	68115	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	57396299	68080	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	1	1	6	2.0	CN1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914758	68080	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	1	1	6	2.0	CN1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	57396300	68097	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	305	1	2	6	1.6	Nc1nc(N2CCNCC2)c2oc3ccc(F)c(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914775	68097	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	305	1	2	6	1.6	Nc1nc(N2CCNCC2)c2oc3ccc(F)c(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	46228161	199877	14	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	233	1	2	6	-0.3	CN1CCN(c2nc(N)nc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594694	199877	14	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	233	1	2	6	-0.3	CN1CCN(c2nc(N)nc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	1223	950	41	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	10023327	168992	0	None	-144	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11615601	74991	0	None	-10	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	1	2	3.0	c1nc(CCCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203390	74991	0	None	-10	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	1	2	3.0	c1nc(CCCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	11600849	140519	0	None	-31	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL381074	140519	0	None	-31	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	66572108	75611	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3c(O)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047266	75611	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3c(O)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	9965450	75617	3	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3ccc(Cl)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047272	75617	3	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3ccc(Cl)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	45268970	196160	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202325	196160	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL561369	196160	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	45267238	196207	0	None	1	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	345	7	4	2	3.5	N=C(NC=C(c1ccccc1)c1ccccc1)NCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL561651	196207	0	None	1	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	345	7	4	2	3.5	N=C(NC=C(c1ccccc1)c1ccccc1)NCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1919	119	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	127050019	140740	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	233	2	2	5	0.3	CN[C@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1039/C6MD00108D
	CHEMBL3818355	140740	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	233	2	2	5	0.3	CN[C@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1039/C6MD00108D
	1194	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	23786499	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	CHEMBL26116	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	127035835	137260	0	None	1	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752270	137260	0	None	1	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	70690368	75649	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	1	1	2	2.3	Cc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047461	75649	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	1	1	2	2.3	Cc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24854707	96266	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	338	3	0	5	3.3	CN1CCN(c2nc3ccccc3nc2Oc2ccc(F)cc2)CC1	10.1021/jm7014217
	CHEMBL261222	96266	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	338	3	0	5	3.3	CN1CCN(c2nc3ccccc3nc2Oc2ccc(F)cc2)CC1	10.1021/jm7014217
	57390224	67848	0	None	-776	3	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	503	8	1	5	4.7	COc1ccc(CN(CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910379	67848	0	None	-776	3	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	503	8	1	5	4.7	COc1ccc(CN(CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	4905344	35306	9	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2cc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL1437043	35306	9	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2cc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1016/j.ejmech.2012.06.016
	70690367	75647	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	3	2.0	COc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047459	75647	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	3	2.0	COc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	59777309	110127	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.3	CN[C@@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1021/jm401727m
	CHEMBL3236581	110127	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.3	CN[C@@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1021/jm401727m
	57400777	68180	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915345	68180	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	56835171	69711	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69711	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	3035746	188156	9	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL1237146	188156	9	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL498770	188156	9	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	53378928	115888	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	289	4	2	5	1.4	CN[C@@H]1CCN(c2cc(CCC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354812	115888	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	289	4	2	5	1.4	CN[C@@H]1CCN(c2cc(CCC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	44547564	117091	2	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	2.2	CN1CCN(c2ccnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393531	117091	2	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	2.2	CN1CCN(c2ccnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	57400777	68180	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915345	68180	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400738	68155	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.5	Nc1nc(N2CC[C@@H](N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915049	68155	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.5	Nc1nc(N2CC[C@@H](N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	56851445	71612	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923025	71612	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963551	71612	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	71603194	89693	0	None	35	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	3	2	5	1.6	CCc1ccc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376797	89693	0	None	35	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	3	2	5	1.6	CCc1ccc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	45257133	199881	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	199881	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	155540438	172517	0	None	7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4483783	172517	0	None	7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	57394625	68288	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	325	7	2	6	1.7	COc1ccccc1Cn1ccc(NCCc2cnc[nH]2)nc1=O	10.1016/j.bmcl.2011.07.114
	CHEMBL1916493	68288	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	325	7	2	6	1.7	COc1ccccc1Cn1ccc(NCCc2cnc[nH]2)nc1=O	10.1016/j.bmcl.2011.07.114
	124087	1377	114	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	7157	1377	114	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	814	1377	114	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	CHEMBL1172	1377	114	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	DB00967	1377	114	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	54587791	61513	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770983	61513	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	10515050	6217	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1081541	6217	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL350478	6217	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	73355143	91841	0	None	-22	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	323	8	2	4	2.8	O=C(NCCOc1ccccc1Cl)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL2413362	91841	0	None	-22	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	323	8	2	4	2.8	O=C(NCCOc1ccccc1Cl)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	56678756	65599	6	None	-	1	Human	5.8	pKi	=	5.8	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4	ChEMBL	391	5	1	4	4.2	CCC(=O)N[C@@H]1CC[C@H](C(=O)N(C)c2ccc(-c3nc4ccccc4o3)cc2)C1	10.6019/CHEMBL5212743
	CHEMBL1834199	65599	6	None	-	1	Human	5.8	pKi	=	5.8	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4	ChEMBL	391	5	1	4	4.2	CCC(=O)N[C@@H]1CC[C@H](C(=O)N(C)c2ccc(-c3nc4ccccc4o3)cc2)C1	10.6019/CHEMBL5212743
	137634349	155991	0	None	-741	2	Human	4.8	pKi	=	4.8	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	6	0	3	4.7	CC(=O)c1ccc(OCc2ccc(CN3CCCC(C)C3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4062122	155991	0	None	-741	2	Human	4.8	pKi	=	4.8	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	6	0	3	4.7	CC(=O)c1ccc(OCc2ccc(CN3CCCC(C)C3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	164626370	186449	0	None	-14	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	6	4	4	1.8	CCCCCCNC(=O)/N=C(\N)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	CHEMBL4876799	186449	0	None	-14	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	6	4	4	1.8	CCCCCCNC(=O)/N=C(\N)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	155568977	176128	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	281	6	3	3	2.6	c1ccc2[nH]c(CNCC[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	CHEMBL4592695	176128	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	281	6	3	3	2.6	c1ccc2[nH]c(CNCC[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	24854706	95949	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)c(Cl)c2)CC1	10.1021/jm7014217
	CHEMBL259536	95949	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)c(Cl)c2)CC1	10.1021/jm7014217
	1278	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1279	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	4908365	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	CHEMBL129198	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	46929554	16722	0	None	11	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	349	5	1	3	4.7	FC(F)(F)c1ccc(SCC(c2ccncc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243367	16722	0	None	11	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	349	5	1	3	4.7	FC(F)(F)c1ccc(SCC(c2ccncc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	4060730	19347	56	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129199	19347	56	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	49841608	69702	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935565	69702	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	1278	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	1279	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	4908365	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	CHEMBL129198	2137	84	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	4060730	19347	56	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129199	19347	56	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	45379805	199405	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	7	1.9	CN1CCN(c2nc(NCCS(=O)(=O)N3CCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591248	199405	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	7	1.9	CN1CCN(c2nc(NCCS(=O)(=O)N3CCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	164585473	184538	0	None	-3	5	Human	5.8	pKi	=	5.8	Binding	Binding affinity to H4 receptor (unknown origin)Binding affinity to H4 receptor (unknown origin)	ChEMBL	503	5	1	2	7.2	O=CN1CCC(c2ccc(-c3cc(C(=O)O)cc4cc(-c5ccc(C(F)(F)F)cc5)ccc34)cc2)CC1	10.1021/acs.jmedchem.1c00164
	CHEMBL4848380	184538	0	None	-3	5	Human	5.8	pKi	=	5.8	Binding	Binding affinity to H4 receptor (unknown origin)Binding affinity to H4 receptor (unknown origin)	ChEMBL	503	5	1	2	7.2	O=CN1CCC(c2ccc(-c3cc(C(=O)O)cc4cc(-c5ccc(C(F)(F)F)cc5)ccc34)cc2)CC1	10.1021/acs.jmedchem.1c00164
	71519177	86455	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314760	86455	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	112936262	137251	1	None	3	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2nc(-c3ccccc3)cc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752195	137251	1	None	3	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2nc(-c3ccccc3)cc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	1403136	180485	9	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL475331	180485	9	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm901379s
	164611143	184692	0	None	-75	4	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	414	6	4	4	2.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(F)(F)F	10.1016/j.ejmech.2021.113190
	CHEMBL4850448	184692	0	None	-75	4	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	414	6	4	4	2.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(F)(F)F	10.1016/j.ejmech.2021.113190
	137646744	157796	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	391	10	1	5	3.0	O=C(CCNC(=O)C(Cc1ccccc1)Cn1ccnc1)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4083336	157796	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	391	10	1	5	3.0	O=C(CCNC(=O)C(Cc1ccccc1)Cn1ccnc1)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	56835171	69711	0	None	-10	9	Rat	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from rat H4RDisplacement of [3H]histamine from rat H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69711	0	None	-10	9	Rat	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from rat H4RDisplacement of [3H]histamine from rat H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	90645149	112147	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1cccc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290574	112147	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1cccc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	46890021	7337	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.8	Cc1cc(OCCCNCC2CCCCN2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086328	7337	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.8	Cc1cc(OCCCNCC2CCCCN2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	118725541	117088	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	1	4	2.0	CCCCNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393528	117088	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	1	4	2.0	CCCCNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	16082858	80063	0	None	-8	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL213133	80063	0	None	-8	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	45379809	199366	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	420	5	1	8	2.1	CN1CCN(c2nc(NCCN3C(=O)CSC3=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591027	199366	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	420	5	1	8	2.1	CN1CCN(c2nc(NCCN3C(=O)CSC3=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	135398737	955	93	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	955	93	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	955	93	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	955	93	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	955	93	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	10575070	67852	0	None	-79	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	439	7	1	3	4.7	O=C(c1cc2ccccc2[nH]1)N1CCN(CCOC(c2ccccc2)c2ccccc2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910383	67852	0	None	-79	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	439	7	1	3	4.7	O=C(c1cc2ccccc2[nH]1)N1CCN(CCOC(c2ccccc2)c2ccccc2)CC1	10.1016/j.bmcl.2011.09.001
	164622330	185838	0	None	-724	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4867962	185838	0	None	-724	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	1267	3775	49	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	3035905	3775	49	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	CHEMBL260374	3775	49	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	122193098	123891	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628045	123891	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645162	112162	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	324	2	1	6	1.3	CN1CCN(c2nc(N)nc(-c3cc(F)c(F)c(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290589	112162	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	324	2	1	6	1.3	CN1CCN(c2nc(N)nc(-c3cc(F)c(F)c(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	24745507	68191	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915538	68191	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	45257133	199881	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	199881	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	24826165	68085	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914763	68085	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	1278	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	1279	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	4908365	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	CHEMBL129198	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	3032915	15463	24	None	-1	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1021/jm0341047
	CHEMBL12160	15463	24	None	-1	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1021/jm0341047
	127052294	140277	0	None	5	4	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	140277	0	None	5	4	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	57394550	68087	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	2	1	7	2.2	COc1ccc2c(c1)sc1c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914765	68087	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	2	1	7	2.2	COc1ccc2c(c1)sc1c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.08.014
	51354009	58853	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cc2cn(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688940	58853	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cc2cn(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	156011119	177098	0	None	-3	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	841	18	4	6	8.7	Cc1cc(OCCCNC(=O)CCCCCNC(=O)COc2ccc(/C=C/C3=[N+]4C(=Cc5ccc(-c6ccc[nH]6)n5[B-]4(F)F)C=C3)cc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4633688	177098	0	None	-3	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	841	18	4	6	8.7	Cc1cc(OCCCNC(=O)CCCCCNC(=O)COc2ccc(/C=C/C3=[N+]4C(=Cc5ccc(-c6ccc[nH]6)n5[B-]4(F)F)C=C3)cc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	51354296	58855	0	None	-4	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	274	6	1	5	1.6	c1nc(CCCn2cc(CN3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688942	58855	0	None	-4	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	274	6	1	5	1.6	c1nc(CCCn2cc(CN3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	1236	2054	25	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	1237	2054	25	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	156615	2054	25	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	CHEMBL268229	2054	25	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	756701	199794	33	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	218	1	1	5	0.1	CN1CCN(c2ncnc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594152	199794	33	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	218	1	1	5	0.1	CN1CCN(c2ncnc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	63868328	174227	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4549015	174227	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	58227381	176592	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4513989	176592	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597817	176592	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25128822	187457	0	None	-10	5	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL494093	187457	0	None	-10	5	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	25179124	174209	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm800876b
	CHEMBL454883	174209	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm800876b
	25179122	193249	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL523941	193249	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179124	174209	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm901379s
	CHEMBL454883	174209	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm901379s
	25179122	193249	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL523941	193249	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1265	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	6422124	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	CHEMBL14638	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	24994634	2129	31	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2129	31	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2129	31	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2129	31	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL4520788	213952	10	None	-89	25	Human	5.7	pKi	=	5.7	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000743a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000743a HRH4	ChEMBL		None	None	None		CNCc1ccccc1-c1csc([C@H](C)Nc2nc(C)nc3cc(OC)c(OC)cc23)c1	10.6019/CHEMBL5212743
	54581937	61493	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	3	1	5	1.3	CC(C)N(C)c1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770963	61493	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	3	1	5	1.3	CC(C)N(C)c1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	137646939	157714	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	335	5	3	4	3.6	CC(C)(CN=C(c1ccccc1O)c1ccccc1O)c1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL4082297	157714	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	335	5	3	4	3.6	CC(C)(CN=C(c1ccccc1O)c1ccccc1O)c1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	9878733	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL338051	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9878733	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338051	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	49841610	69703	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3cc(-c4ccccc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935566	69703	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3cc(-c4ccccc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	44547127	115892	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	44547127	115892	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115892	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354816	115892	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	24739219	68024	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	289	1	2	6	1.8	Nc1nc(N2CC(N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914546	68024	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	289	1	2	6	1.8	Nc1nc(N2CC(N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	24826265	68083	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.3	CN1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914761	68083	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.3	CN1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	9992865	114400	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL333220	114400	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	9878733	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338051	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	2063135	8346	9	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093180	8346	9	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	44129213	199360	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)c(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL590995	199360	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)c(Cl)cc3n2)CC1	10.1021/jm901379s
	71525681	89699	0	None	-2	6	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89699	0	None	-2	6	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	136141537	56451	3	None	2	3	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56451	3	None	2	3	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	136030655	56452	0	None	-2	3	Rat	7.7	pKi	=	7.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56452	0	None	-2	3	Rat	7.7	pKi	=	7.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1278	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1279	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	4908365	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL129198	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1251	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	3035842	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL18661	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1251	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	3035842	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	CHEMBL18661	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	1251	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	3035842	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	CHEMBL18661	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	11673377	139237	0	None	-1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	263	7	2	2	3.2	Clc1ccc(CNCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	CHEMBL379027	139237	0	None	-1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	263	7	2	2	3.2	Clc1ccc(CNCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	25129523	187263	45	None	1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	187263	45	None	1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	66572114	75621	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047276	75621	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70690369	75661	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	1	2	2	2.0	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047473	75661	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	1	2	2	2.0	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	9878733	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL338051	116818	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1204	1919	119	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1247	1919	119	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1375	1919	119	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	774	1919	119	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	CHEMBL90	1919	119	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	DB05381	1919	119	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1278	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2137	84	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	11846298	139256	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	CHEMBL379102	139256	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	155538989	176305	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4513421	176305	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595438	176305	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	164608815	184430	0	None	-47	6	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	7	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4846638	184430	0	None	-47	6	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	7	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1016/j.ejmech.2021.113190
	164611342	185064	0	None	-416	6	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	346	6	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856005	185064	0	None	-416	6	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	346	6	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1016/j.ejmech.2021.113190
	155513612	176296	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156015838	178222	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4640263	178222	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650622	178222	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	127036982	137442	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	525	6	1	6	5.9	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3c2CCc2ccccc2-3)cc1-c1ccc(Cl)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753784	137442	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	525	6	1	6	5.9	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3c2CCc2ccccc2-3)cc1-c1ccc(Cl)cc1	10.1016/j.bmcl.2015.12.035
	57393769	68141	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	281	1	2	6	1.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915030	68141	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	281	1	2	6	1.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	11688307	69874	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938968	69874	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136141537	56451	3	None	-10	3	Rat	6.7	pKi	=	6.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56451	3	None	-10	3	Rat	6.7	pKi	=	6.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	44395364	66869	0	None	15	2	Human	6.7	pKi	=	6.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	276	2	1	2	3.3	CN1CCC(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186456	66869	0	None	15	2	Human	6.7	pKi	=	6.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	276	2	1	2	3.3	CN1CCC(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	10037650	5177	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106158	5177	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	135398737	955	93	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	38	955	93	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	722	955	93	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	CHEMBL42	955	93	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	DB00363	955	93	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	11572015	75001	0	None	-50	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	9	2	2	2.5	CCCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL203441	75001	0	None	-50	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	9	2	2	2.5	CCCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11600780	76020	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	2	2	1.7	CC(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205732	76020	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	2	2	1.7	CC(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	1408942	65093	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	254	2	0	4	1.9	CN1CCN(c2cc(-c3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824046	65093	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	254	2	0	4	1.9	CN1CCN(c2cc(-c3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.06.123
	4060730	19347	56	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19347	56	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	25129527	187390	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	187390	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	66573462	75641	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	2	2	2	2.2	CNC1CN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047453	75641	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	2	2	2	2.2	CNC1CN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	767246	95988	5	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	242	1	0	4	1.7	Cc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL259745	95988	5	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	242	1	0	4	1.7	Cc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	155550732	176605	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4540849	176605	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4597895	176605	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	11313837	2483	8	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1254	2483	8	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL175782	2483	8	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	11313837	2483	8	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	1254	2483	8	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	CHEMBL175782	2483	8	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	71681480	89632	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	307	6	3	7	2.0	Nc1nc(NCCNc2ccncn2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375761	89632	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	307	6	3	7	2.0	Nc1nc(NCCNc2ccncn2)cc(-c2ccccc2)n1	10.1021/jm301886t
	57392460	69837	0	None	14	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL1938843	69837	0	None	14	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572104	75607	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ccncc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047262	75607	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ccncc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572112	75619	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3ccc([N+](=O)[O-])cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047274	75619	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3ccc([N+](=O)[O-])cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	44395361	66910	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	293	1	2	3	2.0	CN1CCN(C(=O)Nc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186640	66910	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	293	1	2	3	2.0	CN1CCN(C(=O)Nc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	10879463	5258	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106570	5258	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10945113	109602	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL322256	109602	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	127035836	137493	0	None	-5	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	4	1	5	3.9	Cc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3754176	137493	0	None	-5	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	4	1	5	3.9	Cc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	164618283	184520	0	None	-537	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	5	2.4	COc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848026	184520	0	None	-537	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	5	2.4	COc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	53233805	61494	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770964	61494	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	54585781	61531	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771000	61531	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	118720442	115895	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	3	5	1.4	Nc1cc(N2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354820	115895	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	3	5	1.4	Nc1cc(N2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	44547268	117086	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393526	117086	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227255	117099	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393539	117099	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	58227252	117104	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393544	117104	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	25112105	8131	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1091875	8131	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737949	68103	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914782	68103	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	24825996	68109	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914788	68109	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	59777341	110121	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	275	3	2	5	1.9	CN[C@@H]1CCN(c2cc(C3CCCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236576	110121	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	275	3	2	5	1.9	CN[C@@H]1CCN(c2cc(C3CCCCC3)nc(N)n2)C1	10.1021/jm401727m
	1278	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	24745335	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	24745335	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	53257796	110120	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236575	110120	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	24738269	68021	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.1	Nc1nc(N2CC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914543	68021	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.1	Nc1nc(N2CC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	1223	950	41	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	44231735	7753	0	None	-1	4	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089390	7753	0	None	-1	4	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	44462956	5567	0	None	2	2	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL1077276	5567	0	None	2	2	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	1278	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1278	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2137	84	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	137657169	159563	0	None	3	2	Human	8.6	pKi	=	8.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	8	1	4	4.1	O=[N+]([O-])c1cccc(C(OCCCc2c[nH]cn2)c2ccccc2)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4102970	159563	0	None	3	2	Human	8.6	pKi	=	8.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	8	1	4	4.1	O=[N+]([O-])c1cccc(C(OCCCc2c[nH]cn2)c2ccccc2)c1	10.1016/j.ejmech.2016.09.074
	59777258	110106	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236561	110106	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	25130914	187230	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL492677	187230	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	46890547	6484	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082772	6484	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	10446295	2119	34	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	1277	2119	34	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	CHEMBL185951	2119	34	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	68081305	110095	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	1	1	2	1.3	CN1CCN(C(=O)c2cc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	CHEMBL3236550	110095	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	1	1	2	1.3	CN1CCN(C(=O)c2cc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	24798144	110098	2	None	-19	3	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	110098	2	None	-19	3	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	24738103	68101	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(OC)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914780	68101	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(OC)c23)C1	10.1016/j.bmcl.2011.08.014
	24745335	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	294	12	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	16362	3103	71	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	2172	3103	71	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	90	3103	71	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	CHEMBL1423	3103	71	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	DB01100	3103	71	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	9904236	14044	22	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL1197564	14044	22	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46890589	6990	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	7	1	4	4.8	Cc1cc(OCCCN2CCCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084809	6990	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	7	1	4	4.8	Cc1cc(OCCCN2CCCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57396382	68290	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	9	2	7	3.8	COCc1ccc(CN2CC[C@H](Nc3cc(NCCC(C)(C)C)ncn3)C2)o1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916495	68290	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	9	2	7	3.8	COCc1ccc(CN2CC[C@H](Nc3cc(NCCC(C)(C)C)ncn3)C2)o1	10.1016/j.bmcl.2011.07.114
	57391122	68291	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	7	3	6	3.9	CC(C)(C)CCNc1cc(N[C@H]2CCN(Cc3cccc(F)c3O)C2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916496	68291	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	7	3	6	3.9	CC(C)(C)CCNc1cc(N[C@H]2CCN(Cc3cccc(F)c3O)C2)ncn1	10.1016/j.bmcl.2011.07.114
	57394628	68296	1	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	4	2	5	1.7	CC(C)(C)CCNc1cc(N2CCNCC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916500	68296	1	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	4	2	5	1.7	CC(C)(C)CCNc1cc(N2CCNCC2)ncn1	10.1016/j.bmcl.2011.07.114
	57397738	69835	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	245	1	0	5	0.1	CN1CCN(C(=O)c2cn3ccncc3n2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938841	69835	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	245	1	0	5	0.1	CN1CCN(C(=O)c2cn3ccncc3n2)CC1	10.1016/j.bmcl.2011.11.098
	10399535	118415	3	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	2	2	1.6	CC1CN(C(=O)c2cc3ccccc3[nH]2)CCN1	10.1021/jm0341047
	CHEMBL341087	118415	3	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	2	2	1.6	CC1CN(C(=O)c2cc3ccccc3[nH]2)CCN1	10.1021/jm0341047
	90645147	112149	0	None	13	3	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290576	112149	0	None	13	3	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	155567108	176612	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4586599	176612	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597946	176612	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	155536509	176448	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4473753	176448	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596582	176448	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	4687476	96282	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL261320	96282	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	9928073	203626	0	None	-67	3	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	343	0	0	4	4.1	CN1CCN(C2=Nc3cc(Cl)ccc3Sc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL67284	203626	0	None	-67	3	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	343	0	0	4	4.1	CN1CCN(C2=Nc3cc(Cl)ccc3Sc3ccccc32)CC1	10.1021/jm051008s
	90654501	110282	0	None	-21877	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	314	6	0	5	3.1	Cc1nnc(C)n1-c1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	CHEMBL3238444	110282	0	None	-21877	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	314	6	0	5	3.1	Cc1nnc(C)n1-c1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	4687476	96282	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1021/jm7014217
	CHEMBL261320	96282	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1021/jm7014217
	10879463	5258	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106570	5258	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	46228132	202122	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL609161	202122	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	46228071	200155	18	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL596562	200155	18	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	51354870	58874	0	None	-26	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	305	6	0	5	2.0	CN1CCN(CCCn2cc(CC3CCCCC3)nn2)CC1	10.1021/jm1013488
	CHEMBL1688981	58874	0	None	-26	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	305	6	0	5	2.0	CN1CCN(CCCn2cc(CC3CCCCC3)nn2)CC1	10.1021/jm1013488
	57391928	69880	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4ccc(Cl)nc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938973	69880	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4ccc(Cl)nc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	23650960	149105	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(F)ccc2[nH]1	nan
	CHEMBL3943309	149105	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(F)ccc2[nH]1	nan
	42624149	110113	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3236568	110113	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1016/j.bmcl.2014.10.013
	25218146	117081	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	2	0	4	2.6	CN1CCN(c2ccnc(-c3ccsc3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393521	117081	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	2	0	4	2.6	CN1CCN(c2ccnc(-c3ccsc3)c2)CC1	10.1016/j.bmcl.2014.12.027
	42624149	110113	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	110113	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	53258370	110114	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236569	110114	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	57394548	68069	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@@H]3CCCN[C@@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914747	68069	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@@H]3CCCN[C@@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	57396298	68074	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	1	1	6	1.8	CN1CCN(c2nc(N)nc3c2oc2cccc(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914752	68074	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	1	1	6	1.8	CN1CCN(c2nc(N)nc3c2oc2cccc(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	44628629	68089	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	269	1	2	6	1.4	Nc1nc(N2CCNCC2)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914767	68089	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	269	1	2	6	1.4	Nc1nc(N2CCNCC2)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	11630326	72542	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199231	72542	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	51354582	58868	0	None	2	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.3	CC(c1cn(CCCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	CHEMBL1688974	58868	0	None	2	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.3	CC(c1cn(CCCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	71603195	89694	0	None	8	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	3	2	5	1.9	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376798	89694	0	None	8	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	3	2	5	1.9	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	1251	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	3035842	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	CHEMBL18661	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	1530	2169	50	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	3827	2169	50	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	7206	2169	50	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	CHEMBL534	2169	50	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	DB00920	2169	50	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	57399107	68185	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1021/acs.jmedchem.5b00516
	CHEMBL1915532	68185	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1021/acs.jmedchem.5b00516
	57399107	68185	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915532	68185	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.125
	57399107	68185	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915532	68185	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.114
	57395619	68186	14	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915533	68186	14	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	57395619	68186	14	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915533	68186	14	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	168268680	192742	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5172675	192742	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221210	192742	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	1403136	180485	9	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL475331	180485	9	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm800876b
	1204	1919	119	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	164610824	185086	0	None	-14	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	452	8	4	4	3.6	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856200	185086	0	None	-14	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	452	8	4	4	3.6	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	10945113	109602	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL322256	109602	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	21896704	159502	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	429	9	1	7	2.7	CC(=O)SCC(Cc1ccc2c(c1)OCO2)C(=O)NCC(=O)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4102243	159502	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	429	9	1	7	2.7	CC(=O)SCC(Cc1ccc2c(c1)OCO2)C(=O)NCC(=O)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	46912441	10870	0	None	-117	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	309	3	1	2	3.9	FC(F)(F)c1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1172754	10870	0	None	-117	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	309	3	1	2	3.9	FC(F)(F)c1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	118725538	117082	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	3	1	4	2.6	CN[C@@H]1CCN(c2ccnc(-c3ccsc3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393522	117082	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	3	1	4	2.6	CN[C@@H]1CCN(c2ccnc(-c3ccsc3)c2)C1	10.1016/j.bmcl.2014.12.027
	1204	1919	119	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	155567030	176393	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4586133	176393	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4596108	176393	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	24854708	155699	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL405416	155699	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	51354587	58873	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	0	5	2.7	c1cn(CCCn2cc(CC3CCCCC3)nn2)cn1	10.1021/jm1013488
	CHEMBL1688979	58873	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	0	5	2.7	c1cn(CCCn2cc(CC3CCCCC3)nn2)cn1	10.1021/jm1013488
	10702196	108541	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL320279	108541	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	136016345	10352	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	377	5	4	6	2.1	CCOC(=O)c1ccc(NC2=CC(=O)/C(=N\NC(=N)N)c3ccccc32)cc1	10.1021/jm7014777
	CHEMBL1162352	10352	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	377	5	4	6	2.1	CCOC(=O)c1ccc(NC2=CC(=O)/C(=N\NC(=N)N)c3ccccc32)cc1	10.1021/jm7014777
	127052293	140213	3	None	34	4	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	140213	3	None	34	4	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1251	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	3035842	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	CHEMBL18661	2001	39	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	11258459	84200	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	CHEMBL220933	84200	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	46228082	202356	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	340	5	2	6	2.5	CC(C)c1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL610626	202356	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	340	5	2	6	2.5	CC(C)c1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	49841721	69695	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	CHEMBL1935558	69695	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	25178551	174207	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454882	174207	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm800876b
	25178551	174207	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454882	174207	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm901379s
	45379807	199486	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	6	3.5	CN1CCN(c2nc(NCCC(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591970	199486	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	6	3.5	CN1CCN(c2nc(NCCC(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10037650	5177	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106158	5177	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	122194624	123994	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccccc1/C=C/c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629827	123994	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccccc1/C=C/c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2015.08.014
	57400700	67849	0	None	-3090	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	470	8	1	6	3.4	COc1ccc(CN(CCN2CCN(C(=O)c3nc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910380	67849	0	None	-3090	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	470	8	1	6	3.4	COc1ccc(CN(CCN2CCN(C(=O)c3nc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	164610421	185236	0	None	-7	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	332	5	4	4	2.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)Nc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4858567	185236	0	None	-7	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	332	5	4	4	2.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)Nc1ccccc1	10.1016/j.ejmech.2021.113190
	46890120	6505	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	416	6	1	4	5.2	Cc1cc(OCCCN2Cc3cccnc3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082851	6505	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	416	6	1	4	5.2	Cc1cc(OCCCN2Cc3cccnc3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	56851604	71600	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923028	71600	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963460	71600	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	24853663	96002	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	272	3	0	5	1.8	CCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL259839	96002	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	272	3	0	5	1.8	CCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	24278976	96222	61	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2nc3ccccc3nc2Cl)CC1	10.1021/jm7014217
	CHEMBL261010	96222	61	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2nc3ccccc3nc2Cl)CC1	10.1021/jm7014217
	24854704	96105	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	CHEMBL260456	96105	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	10923241	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128958	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10923241	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128958	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0341047
	56835171	69711	0	None	-1	9	Mouse	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from mouse H4RDisplacement of [3H]histamine from mouse H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69711	0	None	-1	9	Mouse	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from mouse H4RDisplacement of [3H]histamine from mouse H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	11687917	14933	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	CHEMBL120854	14933	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	10923241	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128958	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	45257131	199885	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	199885	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	57403350	68293	0	None	35	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	3	1	5	1.7	CN1CCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916498	68293	0	None	35	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	3	1	5	1.7	CN1CCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	24804058	144072	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	424	7	2	5	5.1	Cc1c(F)ccc2[nH]c(-c3cnc(NCCCC4CCN(C)CC4)nc3C(C)C)nc12	nan
	CHEMBL3903520	144072	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	424	7	2	5	5.1	Cc1c(F)ccc2[nH]c(-c3cnc(NCCCC4CCN(C)CC4)nc3C(C)C)nc12	nan
	126963284	144749	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL3909134	144749	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46890675	7210	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	1	4	4.4	Cc1cc(OCCCN2CC3CCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085803	7210	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	1	4	4.4	Cc1cc(OCCCN2CC3CCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	49841722	69696	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3c(-c4ccc(C#N)cc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935559	69696	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3c(-c4ccc(C#N)cc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	168276867	192811	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174278	192811	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221653	192811	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	24853598	96194	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	350	4	0	6	3.2	COc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL260851	96194	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	350	4	0	6	3.2	COc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	24853596	96269	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	354	3	0	5	3.8	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	CHEMBL261225	96269	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	354	3	0	5	3.8	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	164610289	185109	0	None	-38	4	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	7	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856602	185109	0	None	-38	4	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	7	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	57399827	68297	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916501	68297	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	90645155	112155	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290582	112155	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	90645155	112155	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL3290582	112155	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	46228114	200153	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596551	200153	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25178131	186914	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	381	5	1	5	3.7	CN1CCN(c2nc(NCCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL490072	186914	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	381	5	1	5	3.7	CN1CCN(c2nc(NCCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	90645152	112152	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	396	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(I)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290579	112152	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	396	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(I)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	49818718	69701	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935564	69701	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	54582867	61529	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770999	61529	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	25147771	174214	0	None	1	3	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	174214	0	None	1	3	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	11440060	207008	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	368	8	1	2	5.4	c1ccc(C(OCCCc2c[nH]cn2)(c2ccccc2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL91576	207008	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	368	8	1	2	5.4	c1ccc(C(OCCCc2c[nH]cn2)(c2ccccc2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	127051301	140175	0	None	5	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	140175	0	None	5	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227216	117114	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393554	117114	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	550981	69002	12	None	2	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	280	5	2	3	2.9	OC(CNC1CC1)Cn1c2ccccc2c2ccccc21	10.1021/jm2011589
	CHEMBL1923533	69002	12	None	2	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	280	5	2	3	2.9	OC(CNC1CC1)Cn1c2ccccc2c2ccccc21	10.1021/jm2011589
	24804056	150628	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	420	8	2	5	5.1	CCCc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(C)ccc2[nH]1	nan
	CHEMBL3955597	150628	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	420	8	2	5	5.1	CCCc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(C)ccc2[nH]1	nan
	71461658	79028	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	4	0.5	CN1CCN(C(=O)c2nc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL2113162	79028	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	4	0.5	CN1CCN(C(=O)c2nc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	4024	2002	20	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	9989505	2002	20	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	CHEMBL82298	2002	20	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	122193101	123894	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.4	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C(F)(F)F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628048	123894	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.4	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C(F)(F)F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	155546701	173631	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	2	1	6	0.8	CN1CCN(c2nc(N)nc(CC(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4534504	173631	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	2	1	6	0.8	CN1CCN(c2nc(N)nc(CC(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	24798596	199844	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594491	199844	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	21193534	11233	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL1179089	11233	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL59679	11233	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	54587789	61506	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(NCCO)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770976	61506	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(NCCO)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	45257131	199885	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	199885	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	90655027	110100	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236555	110100	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	66740898	110107	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	CHEMBL3236562	110107	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	24826082	68112	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914791	68112	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	11587279	125025	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL364400	125025	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	1226	2006	20	None	2	7	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	41376	2006	20	None	2	7	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	CHEMBL12608	2006	20	None	2	7	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	10105117	104168	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL309819	104168	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10466840	204458	0	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CC/N=C(\S)NCCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72424	204458	0	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CC/N=C(\S)NCCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1247	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1375	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	774	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	CHEMBL90	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	DB05381	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	71681476	89627	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	316	8	4	8	1.5	CC(C)CCc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375756	89627	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	316	8	4	8	1.5	CC(C)CCc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	4060730	19347	56	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm301886t
	CHEMBL129199	19347	56	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm301886t
	10923241	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL128958	19133	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	4060730	19347	56	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129199	19347	56	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	22660616	75618	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3ccc(O)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047273	75618	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3ccc(O)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	71525588	89701	0	None	33	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.1	CNC1CN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376805	89701	0	None	33	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.1	CNC1CN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846300	80237	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm7014217
	CHEMBL213912	80237	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm7014217
	45271470	195027	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202329	195027	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL549481	195027	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	45270644	195449	0	None	3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	257	6	4	2	1.7	N=C(NCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmc.2009.04.007
	CHEMBL552694	195449	0	None	3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	257	6	4	2	1.7	N=C(NCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmc.2009.04.007
	45271464	195480	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202328	195480	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL553455	195480	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10468601	195568	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202324	195568	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL555434	195568	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45268095	196017	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202323	196017	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560313	196017	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1247	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1375	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	774	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	CHEMBL90	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	DB05381	1919	119	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1274	4033	16	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	3063228	4033	16	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	CHEMBL1196470	4033	16	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	10988959	118082	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL340740	118082	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1039/C2MD20212C
	21638785	188634	5	None	-4	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188634	5	None	-4	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	127052294	140277	0	None	5	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	140277	0	None	5	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11751419	137856	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL376268	137856	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	11751419	137856	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2006.08.117
	CHEMBL376268	137856	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2006.08.117
	57402512	68138	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915022	68138	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	46228092	199925	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	299	4	1	6	1.4	CN1CCN(c2cc(OCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594973	199925	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	299	4	1	6	1.4	CN1CCN(c2cc(OCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	4024	2002	20	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	9989505	2002	20	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL82298	2002	20	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	1252	2004	21	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	9793868	2004	21	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL417096	2004	21	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1236	2054	25	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1237	2054	25	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	156615	2054	25	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL268229	2054	25	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1252	2004	21	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	9793868	2004	21	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	CHEMBL417096	2004	21	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	81371	12738	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187946	12738	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	CHEMBL534494	12738	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	52128564	75604	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	0	3	2.5	CN1CCN(C(=O)c2cc3cc(Cl)ccc3o2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047259	75604	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	0	3	2.5	CN1CCN(C(=O)c2cc3cc(Cl)ccc3o2)CC1	10.1016/j.ejmech.2012.06.016
	70684084	75640	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	2	2	2.6	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047452	75640	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	2	2	2.6	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	70692528	75646	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047458	75646	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70686224	75660	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	2	2.4	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047472	75660	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	2	2.4	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	51355156	58880	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	3.1	c1nc(CCCc2cn(C3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688989	58880	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	3.1	c1nc(CCCc2cn(C3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	9990475	56447	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	CHEMBL1632408	56447	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	CHEMBL2448450	56447	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	10583206	109333	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321920	109333	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	71681640	89634	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nc(Cl)cc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375763	89634	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nc(Cl)cc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	12438303	75603	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	260	1	0	3	2.3	CN1CCN(C(=O)c2cc3ccccc3s2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047258	75603	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	260	1	0	3	2.3	CN1CCN(C(=O)c2cc3ccccc3s2)CC1	10.1016/j.ejmech.2012.06.016
	22660598	75628	1	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047440	75628	1	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24854636	156293	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	296	1	0	4	2.4	CN1CCN(c2nc3ccccc3nc2C(F)(F)F)CC1	10.1021/jm7014217
	CHEMBL406558	156293	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	296	1	0	4	2.4	CN1CCN(c2nc3ccccc3nc2C(F)(F)F)CC1	10.1021/jm7014217
	10583206	109333	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321920	109333	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	44395263	66673	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	292	2	2	3	1.7	CN1CCN(NC(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL185546	66673	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	292	2	2	3	1.7	CN1CCN(NC(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	44395336	66760	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	280	4	1	3	1.9	CN(C)CCN(C)C(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL185948	66760	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	280	4	1	3	1.9	CN(C)CCN(C)C(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	44395290	125491	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	279	4	1	2	2.5	CN(C)CCN(C)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL364629	125491	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	279	4	1	2	2.5	CN(C)CCN(C)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	15984057	140096	0	None	-35481	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	348	5	0	2	5.3	c1cc(-c2ccc(CN3CCCCC3)cc2)ccc1CN1CCCCC1	10.1016/j.bmc.2008.10.029
	CHEMBL380101	140096	0	None	-35481	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	348	5	0	2	5.3	c1cc(-c2ccc(CN3CCCCC3)cc2)ccc1CN1CCCCC1	10.1016/j.bmc.2008.10.029
	71519348	86461	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	3	2	6	1.5	Nc1nc(N2CCNCC2)c2ccn(Cc3ccccc3)c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314766	86461	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	3	2	6	1.5	Nc1nc(N2CCNCC2)c2ccn(Cc3ccccc3)c2n1	10.1016/j.bmcl.2012.10.139
	122193106	123898	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	335	3	2	6	1.9	CN1CCN(c2nc(N)nc(/C=C/c3c[nH]c4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628052	123898	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	335	3	2	6	1.9	CN1CCN(c2nc(N)nc(/C=C/c3c[nH]c4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	122193095	123888	0	None	3	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123888	0	None	3	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	54585776	61491	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(N)nc(NCCO)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770961	61491	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(N)nc(NCCO)n2)C1	10.1016/j.bmcl.2011.03.017
	24994630	110104	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	2	5	1.6	Nc1nc(C2CCCC2)cc(N2CCC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236559	110104	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	2	5	1.6	Nc1nc(C2CCCC2)cc(N2CCC[C@@H](N)C2)n1	10.1021/jm401727m
	25178933	186913	1	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL490071	186913	1	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178933	186913	1	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL490071	186913	1	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	122193103	123896	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	341	4	1	8	1.3	CN1CCN(c2nc(N)nc(/C=C/c3cccc([N+](=O)[O-])c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628050	123896	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	341	4	1	8	1.3	CN1CCN(c2nc(N)nc(/C=C/c3cccc([N+](=O)[O-])c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	10446295	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	1277	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL185951	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	10446295	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	1277	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	CHEMBL185951	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	46890627	6557	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083098	6557	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11340020	84234	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	CHEMBL221210	84234	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	136042265	69881	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938974	69881	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11340020	84234	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL221210	84234	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	11189343	141952	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	420	6	1	4	4.9	Cc1cc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)ccc1OCCCN1CCN(C)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL387342	141952	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	420	6	1	4	4.9	Cc1cc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)ccc1OCCCN1CCN(C)CC1	10.1016/j.bmcl.2006.08.117
	57394549	68070	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914748	68070	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	10446295	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2119	34	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	24759624	96103	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	0	5	2.0	COc1nc2cc(Cl)ccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL260444	96103	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	0	5	2.0	COc1nc2cc(Cl)ccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	25147771	174214	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	174214	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	44129211	200952	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	383	5	1	7	1.5	CN1CCN(c2nc(NCCS(C)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL601946	200952	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	383	5	1	7	1.5	CN1CCN(c2nc(NCCS(C)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11821243	18820	8	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	2	1	2	1.9	CCN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128134	18820	8	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	2	1	2	1.9	CCN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	155549319	176300	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4538671	176300	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595399	176300	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	164620704	186059	0	None	-17	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	8	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)c1ccc(C(C)C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4871482	186059	0	None	-17	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	8	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)c1ccc(C(C)C)cc1	10.1016/j.ejmech.2021.113190
	127026016	137464	0	None	-13	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753967	137464	0	None	-13	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	71518861	86468	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	5	2.1	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314774	86468	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	5	2.1	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	122193102	123895	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	321	3	1	7	1.2	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628049	123895	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	321	3	1	7	1.2	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	63868018	175352	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	208	1	1	6	-0.5	Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4574940	175352	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	208	1	1	6	-0.5	Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71518536	86465	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	3	5	0.3	Cc1cc2c(N3CCNCC3)nc(N)nc2[nH]1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314771	86465	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	3	5	0.3	Cc1cc2c(N3CCNCC3)nc(N)nc2[nH]1	10.1016/j.bmcl.2012.10.139
	57391927	69875	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938969	69875	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	3991311	136836	9	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	296	2	0	5	2.7	CCc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	CHEMBL3741986	136836	9	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	296	2	0	5	2.7	CCc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	25178556	189061	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL509961	189061	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	44563707	202575	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccc(Cl)cc3nc2OCc2ccccc2)CC1	10.1021/jm800876b
	CHEMBL612059	202575	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccc(Cl)cc3nc2OCc2ccccc2)CC1	10.1021/jm800876b
	24853595	160147	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2cccc(Cl)c2)CC1	10.1021/jm7014217
	CHEMBL410925	160147	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2cccc(Cl)c2)CC1	10.1021/jm7014217
	155535098	176362	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4471442	176362	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595908	176362	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	10782821	96193	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	332	4	0	4	3.4	CCN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260850	96193	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	332	4	0	4	3.4	CCN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	164616851	184926	0	None	-446	5	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4853772	184926	0	None	-446	5	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	10591811	159329	0	None	4	2	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	9	1	2	4.7	c1ccc(-c2ccc(CCCOCCCc3c[nH]cn3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4100315	159329	0	None	4	2	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	9	1	2	4.7	c1ccc(-c2ccc(CCCOCCCc3c[nH]cn3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	1267	3775	49	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	3035905	3775	49	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	CHEMBL260374	3775	49	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	24804219	148539	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	378	6	2	5	4.2	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cccc2[nH]1	nan
	CHEMBL3938862	148539	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	378	6	2	5	4.2	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cccc2[nH]1	nan
	11565330	97191	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	CHEMBL268525	97191	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	1267	3775	49	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	3035905	3775	49	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	CHEMBL260374	3775	49	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	71525681	89699	0	None	-3	6	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89699	0	None	-3	6	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846634	77980	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209758	77980	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	57395496	68144	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915034	68144	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	51354302	58864	0	None	1	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688970	58864	0	None	1	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25130235	187563	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187563	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187563	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187563	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	57390225	67853	0	None	-3235	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	9	2	5	4.1	COc1ccc(CN(CCN[C@H]2CCN(C(=O)c3cc4ccccc4[nH]3)C2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910384	67853	0	None	-3235	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	9	2	5	4.1	COc1ccc(CN(CCN[C@H]2CCN(C(=O)c3cc4ccccc4[nH]3)C2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	55251397	173240	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	2	1	6	0.3	CC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4525008	173240	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	2	1	6	0.3	CC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	53233803	61492	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770962	61492	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	11293460	84814	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	434	6	1	4	5.1	CN1CCN(CCCOc2ccc(-c3nc4c(ccc5ccccc54)[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL223490	84814	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	434	6	1	4	5.1	CN1CCN(CCCOc2ccc(-c3nc4c(ccc5ccccc54)[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	42624148	110119	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236574	110119	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	11846300	80237	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm800876b
	CHEMBL213912	80237	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm800876b
	11846300	80237	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	80237	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	217674	179257	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL473098	179257	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	217674	179257	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL473098	179257	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	156009715	178296	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634631	178296	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651103	178296	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	45257134	199920	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	CHEMBL594949	199920	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	155539562	172819	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	304	4	1	6	1.7	CN1CCN(c2nc(N)nc(CCC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4514667	172819	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	304	4	1	6	1.7	CN1CCN(c2nc(N)nc(CCC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	10083188	140909	3	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL382276	140909	3	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	9927983	79823	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL1795042	79823	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL212212	79823	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	49841612	69705	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3cc(-c4ccc(C#N)cc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935568	69705	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3cc(-c4ccc(C#N)cc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	54587786	61500	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770970	61500	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	24825897	68081	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	1	1	6	2.2	CN1CCN(c2nc(N)nc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914759	68081	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	1	1	6	2.2	CN1CCN(c2nc(N)nc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	71519674	89700	0	None	-3	5	Rat	7.5	pKi	=	7.5	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89700	0	None	-3	5	Rat	7.5	pKi	=	7.5	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	56851610	71598	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923031	71598	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963448	71598	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	127049856	140220	0	None	2	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805431	140220	0	None	2	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	10680721	5949	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1080089	5949	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL158085	5949	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	753256	179326	12	None	2	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL473662	179326	12	None	2	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228133	200031	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595639	200031	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	29136870	68992	7	None	-46	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	301	2	0	7	0.7	CN1CCN(c2nc(N3CCCC3)c3cnn(C)c3n2)CC1	10.1021/jm2011589
	CHEMBL1923522	68992	7	None	-46	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	301	2	0	7	0.7	CN1CCN(c2nc(N3CCCC3)c3cnn(C)c3n2)CC1	10.1021/jm2011589
	71519176	86454	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	2	5	2.5	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314759	86454	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	2	5	2.5	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	155545717	173445	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	238	3	2	2	2.6	c1ccc2[nH]c(C[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	CHEMBL4529871	173445	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	238	3	2	2	2.6	c1ccc2[nH]c(C[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	156015791	178220	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4646286	178220	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650609	178220	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	24854638	155171	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL402470	155171	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCc2ccccc2)CC1	10.1021/jm7014217
	24854702	96028	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2cccnc2)CC1	10.1021/jm7014217
	CHEMBL259955	96028	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2cccnc2)CC1	10.1021/jm7014217
	24853592	156133	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	376	7	0	5	3.8	CN1CCN(c2nc3ccccc3nc2OCCCCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL406380	156133	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	376	7	0	5	3.8	CN1CCN(c2nc3ccccc3nc2OCCCCc2ccccc2)CC1	10.1021/jm7014217
	122193104	123862	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3627724	123862	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	137632569	156248	0	None	4	2	Human	8.5	pKi	=	8.5	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	418	7	1	2	4.8	Ic1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4065099	156248	0	None	4	2	Human	8.5	pKi	=	8.5	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	418	7	1	2	4.8	Ic1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	53233804	61496	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770966	61496	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	54583873	61499	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770969	61499	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54584824	61507	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770977	61507	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	54585780	61528	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770998	61528	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	58227326	117102	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393542	117102	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	9839217	72490	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199062	72490	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	54583873	61499	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL1770969	61499	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	54585781	61531	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	CHEMBL1771000	61531	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	71551636	89644	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	263	4	1	5	1.4	CC(C)CCc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375772	89644	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	263	4	1	5	1.4	CC(C)CCc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	25129527	187390	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm301886t
	CHEMBL493720	187390	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm301886t
	46881855	7714	0	None	15	4	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089040	7714	0	None	15	4	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	10967174	19410	3	None	2	2	Rat	8.5	pKi	=	8.5	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19410	3	None	2	2	Rat	8.5	pKi	=	8.5	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	46890166	7284	0	None	4786	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7284	0	None	4786	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1278	2137	84	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	71603136	89686	0	None	46	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	3	2	5	2.3	CNC1CN(c2nc(N)nc3cc(C4CCCC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376790	89686	0	None	46	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	3	2	5	2.3	CNC1CN(c2nc(N)nc3cc(C4CCCC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25109291	191362	10	None	-79	8	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	CHEMBL519240	191362	10	None	-79	8	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	127050309	140824	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	283	2	2	6	1.8	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1039/C6MD00108D
	CHEMBL3819470	140824	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	283	2	2	6	1.8	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1039/C6MD00108D
	53476465	68025	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	2	2	6	2.0	NCC1CN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914547	68025	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	2	2	6	2.0	NCC1CN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737947	68098	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	283	2	2	6	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914776	68098	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	283	2	2	6	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1016/j.bmcl.2011.08.014
	25071004	192694	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192694	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	25071004	192694	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192694	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	11957573	1997	34	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	1250	1997	34	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	3692	1997	34	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL19439	1997	34	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	24826358	68067	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	1	2	6	2.4	Nc1nc(N2CC3CCNC3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914745	68067	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	1	2	6	2.4	Nc1nc(N2CC3CCNC3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25101945	193082	0	None	3	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	193082	0	None	3	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	58227401	176427	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	176427	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	176427	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	25068753	188414	19	None	1	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	188414	19	None	1	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1278	2137	84	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1267	3775	49	None	-33	28	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3775	49	None	-33	28	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3775	49	None	-33	28	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	21638785	188634	5	None	4	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188634	5	None	4	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	24994636	110110	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	CHEMBL3236565	110110	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	24826441	68076	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1cccc2oc3c(N4CCN(C)CC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914754	68076	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1cccc2oc3c(N4CCN(C)CC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	10244243	141288	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL383384	141288	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	73353369	89684	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	6	0.4	CN1CCN(c2nc(N)nc3cccnc23)CC1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376788	89684	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	6	0.4	CN1CCN(c2nc(N)nc3cccnc23)CC1	10.1016/j.bmcl.2013.02.091
	11846636	77923	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209628	77923	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	155513612	176296	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	176296	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	176296	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	176296	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	206876	18913	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL128637	18913	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	206876	18913	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128637	18913	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	11249610	157943	0	None	-6309	2	Human	4.5	pKi	=	4.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	354	9	2	5	3.6	Nc1ccc(NCCc2ccc(OCCCN3CCCCC3)cc2)nc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4084820	157943	0	None	-6309	2	Human	4.5	pKi	=	4.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	354	9	2	5	3.6	Nc1ccc(NCCc2ccc(OCCCN3CCCCC3)cc2)nc1	10.1016/j.ejmech.2016.09.074
	137634777	155952	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	453	8	3	6	1.0	CCOC(=O)CN1Cc2ccccc2C[C@@H](NC(=O)C(Cc2ccccc2)C(=O)NO)C1=O	10.1016/j.ejmech.2016.09.074
	CHEMBL4061504	155952	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	453	8	3	6	1.0	CCOC(=O)CN1Cc2ccccc2C[C@@H](NC(=O)C(Cc2ccccc2)C(=O)NO)C1=O	10.1016/j.ejmech.2016.09.074
	164611474	185428	0	None	-478	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	6	4	5	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccs1	10.1016/j.ejmech.2021.113190
	CHEMBL4861704	185428	0	None	-478	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	6	4	5	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccs1	10.1016/j.ejmech.2021.113190
	164619427	186045	0	None	-7	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	5	4	4	3.1	N/C(=N\C(=O)NCCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4871221	186045	0	None	-7	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	5	4	4	3.1	N/C(=N\C(=O)NCCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	164627774	186541	0	None	-58	6	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	344	3	4	4	1.4	N/C(=N\C(=O)NCc1ccccc1)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	CHEMBL4878215	186541	0	None	-58	6	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	344	3	4	4	1.4	N/C(=N\C(=O)NCc1ccccc1)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	24994634	2129	31	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2129	31	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2129	31	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2129	31	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	155552341	176360	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543928	176360	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595906	176360	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	44129283	201004	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3ccc(Cl)c(Cl)c3n2)CC1	10.1021/jm901379s
	CHEMBL602330	201004	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3ccc(Cl)c(Cl)c3n2)CC1	10.1021/jm901379s
	11846299	139195	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	CHEMBL378788	139195	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	10934567	19226	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	CHEMBL129026	19226	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	10934567	19226	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL129026	19226	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	25128833	188378	38	None	-4	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL501882	188378	38	None	-4	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1016/j.bmcl.2011.10.104
	24826354	68111	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914790	68111	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	10934567	19226	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL129026	19226	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	16006947	139032	0	None	1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL378619	139032	0	None	1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	136030655	56452	0	None	-3	3	Human	7.5	pKi	=	7.5	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56452	0	None	-3	3	Human	7.5	pKi	=	7.5	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	9923162	18908	6	None	199	2	Human	7.5	pKi	=	7.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL128613	18908	6	None	199	2	Human	7.5	pKi	=	7.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	170389	206958	35	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634086	206958	35	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL91353	206958	35	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1274	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	3063228	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	CHEMBL1196470	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	170389	206958	35	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL4634086	206958	35	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL91353	206958	35	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681808	89619	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	3	8	3.1	CC(C)CNc1nccc(NCCN(C)c2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375748	89619	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	3	8	3.1	CC(C)CNc1nccc(NCCN(C)c2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	1274	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	3063228	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	CHEMBL1196470	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	56851608	71601	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	CHEMBL1923030	71601	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	CHEMBL1963461	71601	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	1274	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	3063228	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	CHEMBL1196470	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	1278	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	66572105	75608	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047263	75608	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572106	75609	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3c(Cl)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047264	75609	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3c(Cl)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573463	75642	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	2	3.1	Cc1cc(Cl)cc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047454	75642	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	2	3.1	Cc1cc(Cl)cc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70696622	75657	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047469	75657	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10988959	118082	4	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL340740	118082	4	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10087533	195987	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202322	195987	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560068	195987	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	46929648	16727	0	None	-6	3	Human	6.5	pKi	=	6.5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	CHEMBL1243395	16727	0	None	-6	3	Human	6.5	pKi	=	6.5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	137650793	157418	0	None	-1096	2	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	484	7	1	4	4.5	O=C(NCCCCN1CCN(c2cccc(Cl)c2Cl)CC1)c1cncc(Br)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4078910	157418	0	None	-1096	2	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	484	7	1	4	4.5	O=C(NCCCCN1CCN(c2cccc(Cl)c2Cl)CC1)c1cncc(Br)c1	10.1016/j.ejmech.2016.09.074
	46912442	10872	0	None	-575	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	271	4	1	3	2.9	COc1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1172775	10872	0	None	-575	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	271	4	1	3	2.9	COc1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	57392460	69837	0	None	14	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938843	69837	0	None	14	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.bmcl.2011.11.098
	136192886	56450	0	None	-6	4	Rat	6.5	pKi	=	6.5	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56450	0	None	-6	4	Rat	6.5	pKi	=	6.5	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1248	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	3077	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	90045	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	CHEMBL12344	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	1239	2020	24	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1240	2020	24	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	912	2020	24	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL268490	2020	24	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	71681976	89622	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	5	4	5	2.0	Nc1nccc(NCCNC(=O)c2cc3cc(Cl)ccc3[nH]2)n1	10.1021/jm301886t
	CHEMBL2375751	89622	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	5	4	5	2.0	Nc1nccc(NCCNC(=O)c2cc3cc(Cl)ccc3[nH]2)n1	10.1021/jm301886t
	10023327	168992	0	None	-144	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11622672	74990	2	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	1	2	2.6	c1nc(CCCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203389	74990	2	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	1	2	2.6	c1nc(CCCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	11679919	75512	0	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204413	75512	0	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11701199	75543	0	None	-3	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	1	2	2.1	CCN(CC)CCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204615	75543	0	None	-3	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	1	2	2.1	CCN(CC)CCCCc1c[nH]cn1	10.1021/jm0504353
	1248	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	3077	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	90045	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	CHEMBL12344	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	4060730	19347	56	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19347	56	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	10377814	75613	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3c(N)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047268	75613	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3c(N)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1248	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	3077	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	90045	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	CHEMBL12344	1412	28	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	4563101	66728	11	None	1	2	Human	5.5	pKi	=	5.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	265	4	2	2	2.1	CN(C)CCNC(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL185842	66728	11	None	1	2	Human	5.5	pKi	=	5.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	265	4	2	2	2.1	CN(C)CCNC(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	71681977	89623	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	344	5	3	5	2.4	CN(CCNc1ccnc(N)n1)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1021/jm301886t
	CHEMBL2375752	89623	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	344	5	3	5	2.4	CN(CCNc1ccnc(N)n1)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1021/jm301886t
	71681479	89631	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	393	9	5	9	2.7	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375760	89631	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	393	9	5	9	2.7	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	3063453	12740	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187950	12740	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL534498	12740	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	112936341	137449	1	None	-12	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2nc(NCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753849	137449	1	None	-12	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2nc(NCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	164628701	186443	0	None	-186	3	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	5	4	4	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4876743	186443	0	None	-186	3	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	5	4	4	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC1CCCCC1	10.1016/j.ejmech.2021.113190
	57402928	69836	0	None	9	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	0	4	1.4	CN1CCN(C(=O)c2cn3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938842	69836	0	None	9	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	0	4	1.4	CN1CCN(C(=O)c2cn3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.11.098
	121238071	167216	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	271	2	1	7	0.3	CN1CCN(c2nc(N)nc(-c3ccncc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4292486	167216	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	271	2	1	7	0.3	CN1CCN(c2nc(N)nc(-c3ccncc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	11120826	18886	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128505	18886	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	11120826	18886	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128505	18886	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0341047
	49841720	69694	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3c(-c4ccccc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935557	69694	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3c(-c4ccccc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	44275465	99193	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL281590	99193	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	46890545	7353	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086460	7353	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11120826	18886	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128505	18886	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	57399829	68301	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	235	7	2	5	1.3	CN(C)CCNc1cc(NCC2CC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916505	68301	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	235	7	2	5	1.3	CN(C)CCNc1cc(NCC2CC2)ncn1	10.1016/j.bmcl.2011.07.114
	44126357	115889	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(N)nc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354813	115889	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(N)nc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	57393772	68148	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Br)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915041	68148	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Br)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	24854637	96290	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc3ccccc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261363	96290	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc3ccccc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	11738520	139241	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	CHEMBL1794919	139241	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	CHEMBL379044	139241	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	60150162	173842	0	None	-524	5	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	479	16	2	4	4.8	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccccc1	10.1039/C8MD00527C
	CHEMBL4539444	173842	0	None	-524	5	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	479	16	2	4	4.8	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccccc1	10.1039/C8MD00527C
	164624107	185698	0	None	-70	6	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	9	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4865765	185698	0	None	-70	6	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	9	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCc1ccccc1	10.1016/j.ejmech.2021.113190
	164608941	184359	0	None	-75	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4845733	184359	0	None	-75	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	57392890	68287	0	None	-1	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	370	7	1	5	2.5	COc1cccc(C(O)(COc2ccccc2C)CN2CCN(C)CC2)c1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916492	68287	0	None	-1	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	370	7	1	5	2.5	COc1cccc(C(O)(COc2ccccc2C)CN2CCN(C)CC2)c1	10.1016/j.bmcl.2011.07.114
	21029584	188842	4	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncnc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL506969	188842	4	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncnc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	1267	3775	49	None	-33	28	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	3035905	3775	49	None	-33	28	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	CHEMBL260374	3775	49	None	-33	28	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	90655029	110108	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.4	Nc1nc(C2CCCC2)cc(N2C[C@@H]3CNC[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236563	110108	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.4	Nc1nc(C2CCCC2)cc(N2C[C@@H]3CNC[C@@H]3C2)n1	10.1021/jm401727m
	71518862	86469	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	342	2	2	5	2.6	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314775	86469	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	342	2	2	5	2.6	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	44547567	117093	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393533	117093	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	24826163	68137	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915020	68137	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	57393773	68150	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	1	7	1.8	CN1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915044	68150	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	1	7	1.8	CN1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	11572877	67050	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL187285	67050	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	44129210	199462	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	7	2	7	1.4	CNS(=O)(=O)CCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591711	199462	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	7	2	7	1.4	CNS(=O)(=O)CCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	156015638	178248	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	178248	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	178248	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25179127	189055	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL509852	189055	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25179127	189055	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL509852	189055	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	71518863	86470	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	338	3	2	6	2.0	COc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314776	86470	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	338	3	2	6	2.0	COc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	8070960	155668	10	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	258	2	0	5	1.4	COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL405044	155668	10	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	258	2	0	5	1.4	COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	25130576	187602	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130576	187602	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187602	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187602	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	86277887	123785	26	None	-	1	Human	5.5	pKi	=	5.5	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL	802	18	0	11	7.4	COc1ccc(CC[C@@H](OC(=O)[C@@H]2CCCCN2C(=O)[C@H](c2cc(OC)c(OC)c(OC)c2)C2CCCCC2)c2cccc(OCCN3CCOCC3)c2)cc1OC	10.6019/CHEMBL5058648
	CHEMBL3623630	123785	26	None	-	1	Human	5.5	pKi	=	5.5	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL	802	18	0	11	7.4	COc1ccc(CC[C@@H](OC(=O)[C@@H]2CCCCN2C(=O)[C@H](c2cc(OC)c(OC)c(OC)c2)C2CCCCC2)c2cccc(OCCN3CCOCC3)c2)cc1OC	10.6019/CHEMBL5058648
	168294445	192986	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208113	192986	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222768	192986	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	25130235	187563	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187563	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187563	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187563	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	46228076	202077	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	354	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(C(C)(C)C)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL608864	202077	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	354	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(C(C)(C)C)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228158	202135	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL609289	202135	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	57402514	68146	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Br)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915039	68146	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Br)cc3c2n1	10.1016/j.bmcl.2011.08.014
	164619039	186124	0	None	-17	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	4	4	4	3.0	N/C(=N\C(=O)NCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4872277	186124	0	None	-17	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	4	4	4	3.0	N/C(=N\C(=O)NCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	57392889	68286	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	379	4	1	5	2.2	CN1CCN(c2cc(C(=O)NCc3cccc(C(F)(F)F)c3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916491	68286	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	379	4	1	5	2.2	CN1CCN(c2cc(C(=O)NCc3cccc(C(F)(F)F)c3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	25130235	187563	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187563	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	57393774	68151	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.4	Nc1nc(N2CCNCC2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915045	68151	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.4	Nc1nc(N2CCNCC2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	10083188	140909	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL382276	140909	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	1278	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	1279	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	4908365	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	CHEMBL129198	2137	84	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	71603137	89687	0	None	23	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	1	2	5	1.9	CC(C)(C)c1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376791	89687	0	None	23	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	1	2	5	1.9	CC(C)(C)c1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25179125	189000	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL509142	189000	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179125	189000	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL509142	189000	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	127051047	140286	0	None	2	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806162	140286	0	None	2	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	7015108	199883	8	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	3	0	7	1.7	CN1CCN(c2ncnc3c2nnn3Cc2ccc(Cl)cc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594729	199883	8	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	3	0	7	1.7	CN1CCN(c2ncnc3c2nnn3Cc2ccc(Cl)cc2)CC1	10.1016/j.bmc.2009.08.059
	46228070	199702	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	271	4	0	5	1.1	CN1CCN(Cc2cn(Cc3ccccc3)nn2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593448	199702	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	271	4	0	5	1.1	CN1CCN(Cc2cn(Cc3ccccc3)nn2)CC1	10.1016/j.bmc.2009.08.059
	122193100	123893	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	314	3	1	6	1.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc(F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628047	123893	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	314	3	1	6	1.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc(F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	155513612	176296	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	127050018	140743	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	221	2	2	5	0.5	CN[C@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1039/C6MD00108D
	CHEMBL3818402	140743	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	221	2	2	5	0.5	CN[C@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1039/C6MD00108D
	24994950	110126	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.5	CN[C@@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1021/jm401727m
	CHEMBL3236580	110126	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.5	CN[C@@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1021/jm401727m
	54583875	61525	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770995	61525	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	90655028	110102	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236557	110102	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@H](N)C2)n1	10.1021/jm401727m
	59777262	110117	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236572	110117	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	155512725	176217	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	176217	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	176217	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	46929553	16721	0	None	-5	2	Human	6.4	pKi	=	6.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2cccnc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243366	16721	0	None	-5	2	Human	6.4	pKi	=	6.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2cccnc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	71519011	86435	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	376	2	2	5	3.3	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)c(Cl)c4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314496	86435	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	376	2	2	5	3.3	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)c(Cl)c4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	24826598	68140	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	2	7	1.9	COc1ccc2oc3c(N4CCC[C@H](N)C4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915027	68140	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	2	7	1.9	COc1ccc2oc3c(N4CCC[C@H](N)C4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	25178939	186826	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm800876b
	CHEMBL489464	186826	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm800876b
	24853529	96094	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	336	3	0	5	3.5	CN1CCN(c2nc3ccccc3nc2Sc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260388	96094	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	336	3	0	5	3.5	CN1CCN(c2nc3ccccc3nc2Sc2ccccc2)CC1	10.1021/jm7014217
	25178939	186826	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm901379s
	CHEMBL489464	186826	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm901379s
	164611757	184879	0	None	-50	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	2.8	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4853107	184879	0	None	-50	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	2.8	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	112936357	137334	1	None	-18	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752978	137334	1	None	-18	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	71519346	86459	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	2	6	-0.0	Cn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314764	86459	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	2	6	-0.0	Cn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	58227144	117115	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2cnnc(NC3CC4CCC3C4)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393555	117115	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2cnnc(NC3CC4CCC3C4)c2)CC1	10.1016/j.bmcl.2014.12.027
	57399018	68153	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	332	1	1	7	2.1	CN1CCCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915047	68153	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	332	1	1	7	2.1	CN1CCCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	16082831	80317	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL214311	80317	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	25178554	173403	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm800876b
	CHEMBL452870	173403	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm800876b
	25178554	173403	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm901379s
	CHEMBL452870	173403	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm901379s
	164610892	185174	0	None	-52	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	8	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CC1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4857560	185174	0	None	-52	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	8	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CC1CCCCC1	10.1016/j.ejmech.2021.113190
	71518864	86471	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	Cc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314777	86471	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	Cc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	122194626	123996	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629829	123996	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2015.08.014
	57399019	68157	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	2	2	7	1.4	NCC1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915051	68157	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	2	2	7	1.4	NCC1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	90645146	112150	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	288	2	1	6	1.0	CN1CCN(c2nc(N)nc(-c3ccc(F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290577	112150	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	288	2	1	6	1.0	CN1CCN(c2nc(N)nc(-c3ccc(F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	54584374	63094	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774590	63094	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789360	63094	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	122193105	123897	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3ccc4ccccc4c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628051	123897	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3ccc4ccccc4c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	921599	19423	51	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0341047
	CHEMBL129660	19423	51	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0341047
	921599	19423	51	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0502081
	CHEMBL129660	19423	51	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0502081
	4060730	19347	56	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL129199	19347	56	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.02.097
	25178553	173402	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm800876b
	CHEMBL452869	173402	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm800876b
	25178553	173402	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm901379s
	CHEMBL452869	173402	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm901379s
	11846465	141907	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	CHEMBL387054	141907	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	56851606	71571	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1923029	71571	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1963244	71571	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	51354877	58879	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCc2cn(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688988	58879	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCc2cn(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	137658819	159286	0	None	-7585	2	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	467	9	1	5	3.9	COc1cc2c(cc1OC)C(C)=C(C(=O)NCCCCN1CCN(c3ccccc3F)CC1)C2	10.1016/j.ejmech.2016.09.074
	CHEMBL4099779	159286	0	None	-7585	2	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	467	9	1	5	3.9	COc1cc2c(cc1OC)C(C)=C(C(=O)NCCCCN1CCN(c3ccccc3F)CC1)C2	10.1016/j.ejmech.2016.09.074
	54584374	63094	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774590	63094	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789360	63094	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	58227207	117111	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393551	117111	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	51354872	60169	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1688983	60169	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1739923	60169	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	68081433	110096	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	248	1	1	3	0.7	CN1CCN(C(=O)c2nc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	CHEMBL3236551	110096	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	248	1	1	3	0.7	CN1CCN(C(=O)c2nc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	57399747	68108	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	2	2	6	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914787	68108	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	2	2	6	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)C1	10.1016/j.bmcl.2011.08.014
	25129169	187157	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	187157	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	25129169	187157	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL492231	187157	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	3032915	15463	24	None	-1	7	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL12160	15463	24	None	-1	7	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681806	89616	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	331	8	4	9	1.0	CC(C)CNc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375667	89616	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	331	8	4	9	1.0	CC(C)CNc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681639	89633	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	321	6	3	7	2.3	Cc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375762	89633	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	321	6	3	7	2.3	Cc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	11492393	4029	6	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	1263	4029	6	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	CHEMBL206346	4029	6	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	11557610	74418	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	7	2	2	2.7	c1nc(CCCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL202744	74418	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	7	2	2	2.7	c1nc(CCCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	11615406	139467	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	2	2	2.3	c1nc(CCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL379447	139467	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	2	2	2.3	c1nc(CCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	66572116	75631	1	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	2	2	1.5	Cc1cccc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047443	75631	1	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	2	2	1.5	Cc1cccc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70681961	75643	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.6	CN1CCCN(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047455	75643	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.6	CN1CCCN(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70696621	75650	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	2	2.2	O=C(c1cc2cccc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047462	75650	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	2	2.2	O=C(c1cc2cccc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	70692531	75663	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.0	Cc1ccc(F)c2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047475	75663	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.0	Cc1ccc(F)c2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24759623	96320	20	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm800876b
	CHEMBL261551	96320	20	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm800876b
	25129169	187157	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	187157	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	24759623	96320	20	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261551	96320	20	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	10429016	196137	0	None	-5	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	366	7	3	3	3.6	N=C(NCCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL561250	196137	0	None	-5	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	366	7	3	3	3.6	N=C(NCCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1223	950	41	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	71519012	86436	0	None	218	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.8	Nc1nc(N2CCNCC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314497	86436	0	None	218	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.8	Nc1nc(N2CCNCC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	10934158	108550	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL320331	108550	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	71681478	89630	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	4	8	3.2	CC(C)CCc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375759	89630	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	4	8	3.2	CC(C)CCc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	11629829	75993	0	None	-19	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	5	1	2	2.2	c1nc(CCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL205583	75993	0	None	-19	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	5	1	2	2.2	c1nc(CCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	25831	12976	28	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	CHEMBL1189742	12976	28	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	CHEMBL539343	12976	28	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	1278	2137	84	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2137	84	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2137	84	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2137	84	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	66573456	75633	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	2	2	2	1.5	CNC1CN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047445	75633	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	2	2	2	1.5	CNC1CN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	43398194	75644	25	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	CN1CCCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047456	75644	25	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	CN1CCCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70694582	75648	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047460	75648	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70686223	75653	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	2	2	2	1.4	CNC1CN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047465	75653	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	2	2	2	1.4	CNC1CN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	70692530	75662	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047474	75662	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1238	2069	13	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	6603865	2069	13	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL11919	2069	13	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	44417445	12770	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1188154	12770	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL535387	12770	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	66572102	75605	1	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ncccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047260	75605	1	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ncccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	24854634	156132	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	400	4	0	4	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2CCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL406379	156132	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	400	4	0	4	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2CCc2ccccc2)CC1	10.1021/jm7014217
	24853528	167058	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	329	6	0	6	1.7	CN(C)CCCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL428964	167058	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	329	6	0	6	1.7	CN(C)CCCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	137659490	159172	0	None	11	2	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	420	7	1	2	6.1	Brc1ccc(C(OCCCc2c[nH]cn2)c2ccc3ccccc3c2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4098585	159172	0	None	11	2	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	420	7	1	2	6.1	Brc1ccc(C(OCCCc2c[nH]cn2)c2ccc3ccccc3c2)cc1	10.1016/j.ejmech.2016.09.074
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	24803709	145905	1	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cc(F)cc2[nH]1	nan
	CHEMBL3917992	145905	1	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cc(F)cc2[nH]1	nan
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	49841846	69709	0	None	18	3	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69709	0	None	18	3	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	44129130	199533	0	None	-2	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL592379	199533	0	None	-2	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.10.104
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	24745337	61509	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770979	61509	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227340	117092	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393532	117092	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	44129133	199362	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	398	6	2	7	1.0	CNS(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591011	199362	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	398	6	2	7	1.0	CNS(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	1223	950	41	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	2790	950	41	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	CHEMBL14690	950	41	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	1223	950	41	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	2790	950	41	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	CHEMBL14690	950	41	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	25129169	187157	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL492231	187157	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	44129134	199485	0	None	22	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	199485	0	None	22	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25129169	187157	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	187157	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	1278	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	1279	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	4908365	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	24745506	68190	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915537	68190	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	136042266	69882	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938975	69882	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	136042268	69884	0	None	186	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938977	69884	0	None	186	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	25068753	188414	19	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	188414	19	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1278	2137	84	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1279	2137	84	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	4908365	2137	84	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	CHEMBL129198	2137	84	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	25068753	188414	19	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	188414	19	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	2063135	8346	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1093180	8346	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	24826437	68068	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@H]3CCCN[C@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914746	68068	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@H]3CCCN[C@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25128823	192693	0	None	2	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192693	0	None	2	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	1204	1919	119	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1919	119	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1919	119	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1919	119	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1919	119	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1919	119	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1278	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	1279	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	4908365	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	CHEMBL129198	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	44129130	199533	0	None	-2	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	199533	0	None	-2	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	54581941	61527	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	288	2	2	6	0.8	Nc1nc(N2CCCC2)cc(N2CC3CCCNC3C2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770997	61527	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	288	2	2	6	0.8	Nc1nc(N2CCCC2)cc(N2CC3CCCNC3C2)n1	10.1016/j.bmcl.2011.03.017
	24826680	68099	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914777	68099	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	11601630	132792	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL370135	132792	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	137640214	156943	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	394	6	1	2	5.5	O=C(NCCCCN1Cc2cccc3cccc(c23)C1)c1ccc2ccccc2c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4072893	156943	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	394	6	1	2	5.5	O=C(NCCCCN1Cc2cccc3cccc(c23)C1)c1ccc2ccccc2c1	10.1016/j.ejmech.2016.09.074
	71519013	86437	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	3	2	5	2.5	CCN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314498	86437	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	3	2	5	2.5	CCN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	168284289	192882	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5194860	192882	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222123	192882	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	3076002	179258	3	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL473099	179258	3	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	3076002	179258	3	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL473099	179258	3	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	90645150	112146	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290573	112146	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	25130576	187602	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130576	187602	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187602	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187602	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	10018335	71983	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL197477	71983	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	57393771	68145	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	311	2	2	7	1.5	COc1ccc2oc3c(N4C[C@@H]5C[C@H]4CN5)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915035	68145	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	311	2	2	7	1.5	COc1ccc2oc3c(N4C[C@@H]5C[C@H]4CN5)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	10545988	63117	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774593	63117	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789558	63117	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	46228071	200155	18	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596562	200155	18	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmc.2009.08.059
	1204	1919	119	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1919	119	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1919	119	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1919	119	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1919	119	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1919	119	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	46890590	6992	0	None	1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6992	0	None	1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57400735	68139	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	2	6	2.9	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915026	68139	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	2	6	2.9	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	56851441	71570	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923023	71570	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963243	71570	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	25147772	173385	9	None	-1	3	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL452847	173385	9	None	-1	3	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2015.12.035
	16061961	199656	0	None	7	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	199656	0	None	7	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10381810	205151	0	None	-2	5	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL7828	205151	0	None	-2	5	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	137647794	157957	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	464	8	1	6	4.2	COc1ccc2c(c1)c(CC(=O)NCCCn1ccnc1)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4085023	157957	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	464	8	1	6	4.2	COc1ccc2c(c1)c(CC(=O)NCCCn1ccnc1)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	10545988	63117	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774593	63117	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789558	63117	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	122193096	123889	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628043	123889	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645154	112154	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	1.9	CN1CCN(c2nc(N)nc(-c3ccc(C(F)(F)F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290581	112154	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	1.9	CN1CCN(c2nc(N)nc(-c3ccc(C(F)(F)F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10777275	5863	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079553	5863	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL156005	5863	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	1267	3775	49	None	-33	28	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	3035905	3775	49	None	-33	28	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	CHEMBL260374	3775	49	None	-33	28	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	127036978	137256	0	None	1	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752225	137256	0	None	1	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	164612037	185353	0	None	-81283	20	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	326	8	4	4	1.8	CCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4860528	185353	0	None	-81283	20	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	326	8	4	4	1.8	CCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	1224	1420	83	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	3100	1420	83	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	8980	1420	83	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	916	1420	83	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	CHEMBL657	1420	83	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	DB01075	1420	83	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	9883231	113359	1	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.0	CN1CCN(C(=O)c2nc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL331773	113359	1	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.0	CN1CCN(C(=O)c2nc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	1267	3775	49	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	3035905	3775	49	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	CHEMBL260374	3775	49	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	1267	3775	49	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	3035905	3775	49	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	CHEMBL260374	3775	49	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	24992735	110128	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	CHEMBL3236582	110128	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	24826081	68084	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914762	68084	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	90645153	112153	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290580	112153	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2014.06.032
	25178344	186862	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489664	186862	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10934158	108550	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL320331	108550	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	24853593	96029	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccncc2)CC1	10.1021/jm7014217
	CHEMBL259956	96029	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccncc2)CC1	10.1021/jm7014217
	54586765	61512	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(NCCO)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770982	61512	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(NCCO)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	44275465	99193	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2013.02.091
	CHEMBL281590	99193	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2013.02.091
	24826080	68086	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	1	1	6	3.2	CN1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914764	68086	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	1	1	6	3.2	CN1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	71519349	86462	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	6	1.8	Cn1c(-c2ccc(F)cc2)cc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314767	86462	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	6	1.8	Cn1c(-c2ccc(F)cc2)cc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	57393770	68142	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915031	68142	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	155534519	176459	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4470527	176459	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596640	176459	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	51354873	58876	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688984	58876	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	51354874	58877	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	335	6	1	4	3.5	Clc1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1Cl	10.1021/jm1013488
	CHEMBL1688985	58877	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	335	6	1	4	3.5	Clc1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1Cl	10.1021/jm1013488
	168292954	192973	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5202592	192973	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222660	192973	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	11779175	63136	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774600	63136	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789801	63136	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164612760	184694	0	None	-38	4	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4850490	184694	0	None	-38	4	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	11779175	63136	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774600	63136	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789801	63136	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	58227085	117105	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393545	117105	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	1267	3775	49	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	3035905	3775	49	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	CHEMBL260374	3775	49	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	11846464	78431	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	CHEMBL211170	78431	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	118720433	115884	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	206	3	1	4	0.8	CCc1cc(N2CC[C@@H](NC)C2)ncn1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354807	115884	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	206	3	1	4	0.8	CCc1cc(N2CC[C@@H](NC)C2)ncn1	10.1016/j.bmcl.2014.10.013
	51354581	58867	0	None	-4	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	275	10	1	4	3.1	CCCCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688973	58867	0	None	-4	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	275	10	1	4	3.1	CCCCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	9877454	205590	2	None	-4	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL81644	205590	2	None	-4	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	1613	2333	53	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	205	2333	53	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	3964	2333	53	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	CHEMBL831	2333	53	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	DB00408	2333	53	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	164609493	184412	0	None	-2630	6	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	350	6	4	5	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccco1	10.1016/j.ejmech.2021.113190
	CHEMBL4846380	184412	0	None	-2630	6	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	350	6	4	5	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccco1	10.1016/j.ejmech.2021.113190
	71518698	86464	2	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	218	1	3	5	-0.1	Nc1nc(N2CCNCC2)c2cc[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314770	86464	2	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	218	1	3	5	-0.1	Nc1nc(N2CCNCC2)c2cc[nH]c2n1	10.1016/j.bmcl.2012.10.139
	4242975	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	CHEMBL128088	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	4242975	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL128088	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	16061961	199656	0	None	7	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	199656	0	None	7	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	11258458	83424	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	CHEMBL219930	83424	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	57398183	68023	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	347	4	3	7	1.8	Nc1nc(N2CC[C@@H](NCCO)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914545	68023	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	347	4	3	7	1.8	Nc1nc(N2CC[C@@H](NCCO)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	4242975	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128088	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	118720438	115890	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	220	3	1	4	1.4	CN[C@@H]1CCN(c2ccnc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354814	115890	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	220	3	1	4	1.4	CN[C@@H]1CCN(c2ccnc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	24854640	96030	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccccn2)CC1	10.1021/jm7014217
	CHEMBL259957	96030	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccccn2)CC1	10.1021/jm7014217
	135589846	141795	3	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	356	2	2	5	3.1	OCCN1CCN(C2=Nc3cc(Cl)ccc3Nc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL386333	141795	3	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	356	2	2	5	3.1	OCCN1CCN(C2=Nc3cc(Cl)ccc3Nc3ccccc32)CC1	10.1021/jm051008s
	24826439	68093	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	2	2	7	1.4	COc1cccc2oc3c(N4CCNCC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914771	68093	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	2	2	7	1.4	COc1cccc2oc3c(N4CCNCC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	10446295	2119	34	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	1277	2119	34	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL185951	2119	34	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	10446295	2119	34	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	1277	2119	34	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL185951	2119	34	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	10023327	168992	0	None	-144	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	1267	3775	49	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	3035905	3775	49	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	CHEMBL260374	3775	49	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	155556721	174530	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	248	2	1	6	0.6	CC(C)=Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4556243	174530	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	248	2	1	6	0.6	CC(C)=Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	24854639	96106	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	5	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260458	96106	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	5	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCCc2ccccc2)CC1	10.1021/jm7014217
	11344458	205175	2	None	-14	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78497	205175	2	None	-14	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	51354588	60141	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	CHEMBL1688980	60141	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	CHEMBL1739759	60141	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	16106	118120	34	None	-20	12	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL340801	118120	34	None	-20	12	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	10.1021/jm051008s
	46881855	7714	0	None	-15	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089040	7714	0	None	-15	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	71519014	86438	0	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	2	5	2.9	CC(C)N1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314499	86438	0	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	2	5	2.9	CC(C)N1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	122193094	123887	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628041	123887	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	155513612	176296	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	176296	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	176296	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	176296	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	45379808	201196	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	362	5	2	6	1.6	CC(=O)NCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL603621	201196	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	362	5	2	6	1.6	CC(=O)NCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	168286426	192902	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5198795	192902	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222241	192902	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	164620139	185764	0	None	-56	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	436	8	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4866734	185764	0	None	-56	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	436	8	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	136042267	69883	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938976	69883	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11623296	116997	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338972	116997	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	11066922	19391	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129436	19391	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	11066922	19391	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129436	19391	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0341047
	49841845	69710	0	None	19	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69710	0	None	19	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	25068753	188414	19	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.10.104
	CHEMBL502347	188414	19	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.10.104
	1223	950	41	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	2790	950	41	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	CHEMBL14690	950	41	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	1278	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	1278	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	1279	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	1279	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	4908365	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	4908365	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	CHEMBL129198	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	CHEMBL129198	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	58227086	117117	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393557	117117	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	1279	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	4908365	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	CHEMBL129198	2137	84	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	2063135	8346	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1021/jm401727m
	CHEMBL1093180	8346	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1021/jm401727m
	23650959	110094	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	410	7	1	4	5.3	Cc1cnc(OCCCCC2CCN(C)CC2)cc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/jm401727m
	CHEMBL3236548	110094	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	410	7	1	4	5.3	Cc1cnc(OCCCCC2CCN(C)CC2)cc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/jm401727m
	11066922	19391	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129436	19391	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	155524115	176523	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	176523	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	176523	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	70696620	75638	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	1	2	3	1.8	CN1CCN(C(=O)c2cc3cc(Cl)cc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047450	75638	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	1	2	3	1.8	CN1CCN(C(=O)c2cc3cc(Cl)cc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	25129523	187263	45	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	187263	45	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	76311195	106024	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	284	2	2	6	1.1	CN1CCN(c2cc(-c3ccc(N)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL3133130	106024	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	284	2	2	6	1.1	CN1CCN(c2cc(-c3ccc(N)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25129523	187263	45	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	187263	45	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25128824	193154	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	193154	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	155524115	176523	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	176523	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	176523	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	25128824	193154	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	193154	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	25071004	192694	0	None	-1	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192694	0	None	-1	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	11705395	16020	0	None	-1	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	CHEMBL1223749	16020	0	None	-1	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	24826162	68113	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914792	68113	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	71519674	89700	0	None	1	5	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89700	0	None	1	5	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	46890072	6878	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084355	6878	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	24994634	2129	31	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2129	31	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2129	31	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2129	31	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2129	31	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2129	31	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	44129056	199577	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	474	7	1	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592663	199577	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	474	7	1	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25128822	187457	0	None	10	5	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	187457	0	None	10	5	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	11554490	16021	0	None	-5	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.3	FC(F)(F)c1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL1223750	16021	0	None	-5	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.3	FC(F)(F)c1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1	10.1016/j.ejmech.2016.09.074
	24797956	110103	3	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	110103	3	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	24825514	186831	0	None	5	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	256	4	1	2	3.6	CC(C)(C)CC(=O)c1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	CHEMBL489493	186831	0	None	5	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	256	4	1	2	3.6	CC(C)(C)CC(=O)c1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	24825515	187293	0	None	-1	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	238	2	1	1	3.4	CC(C)(C)C#Cc1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	CHEMBL493014	187293	0	None	-1	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	238	2	1	1	3.4	CC(C)(C)C#Cc1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	11301441	96125	10	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm800876b
	CHEMBL260549	96125	10	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm800876b
	11301441	96125	10	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL260549	96125	10	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm7014217
	24825791	68026	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914548	68026	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25109291	191362	10	None	-501	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	CHEMBL519240	191362	10	None	-501	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	45379922	199540	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	552	7	2	7	2.8	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(I)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592434	199540	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	552	7	2	7	2.8	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(I)ccc3n2)CC1	10.1021/jm901379s
	54585777	61504	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	5	3	5	2.1	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770974	61504	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	5	3	5	2.1	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)c2)C1	10.1016/j.bmcl.2011.03.017
	118720441	115894	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	3	4	2.5	Nc1cc(C2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354819	115894	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	3	4	2.5	Nc1cc(C2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	24826518	68075	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2cccc(C(F)(F)F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914753	68075	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2cccc(C(F)(F)F)c23)CC1	10.1016/j.bmcl.2011.08.014
	1278	2137	84	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2137	84	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2137	84	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2137	84	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	136192886	56450	0	None	1	4	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56450	0	None	1	4	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136141537	56451	3	None	-2	3	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56451	3	None	-2	3	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	71525678	89696	0	None	-5	6	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89696	0	None	-5	6	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	1267	3775	49	None	-14	28	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	3035905	3775	49	None	-14	28	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	CHEMBL260374	3775	49	None	-14	28	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	1223	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1269	121	41	None	213	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	41	None	213	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	41	None	213	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	1255	3173	19	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	6421522	3173	19	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL19173	3173	19	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	10017000	204462	0	None	1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72431	204462	0	None	1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1223	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	950	41	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	71681644	89638	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	412	9	4	8	3.8	CC(C)CNc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375767	89638	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	412	9	4	8	3.8	CC(C)CNc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	71681805	89643	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	426	9	3	8	3.8	CC(C)CNc1nccc(NCCN(C)c2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375771	89643	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	426	9	3	8	3.8	CC(C)CNc1nccc(NCCN(C)c2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	11506928	74413	0	None	-1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	2	2	3.0	c1nc(CCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL202728	74413	0	None	-1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	2	2	3.0	c1nc(CCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	11600779	76019	0	None	-6	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205731	76019	0	None	-6	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	11536119	138682	0	None	-15	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	249	7	2	2	3.4	c1nc(CCCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL377954	138682	0	None	-15	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	249	7	2	2	3.4	c1nc(CCCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	4242975	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL128088	18810	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	45379921	199180	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	426	6	1	7	3.6	CN1CCN(c2nc(NCCc3ccc([N+](=O)[O-])cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL589800	199180	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	426	6	1	7	3.6	CN1CCN(c2nc(NCCc3ccc([N+](=O)[O-])cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	9972181	195194	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202331	195194	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL550757	195194	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10063835	63093	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774601	63093	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789356	63093	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	46890200	7128	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	442	9	1	4	5.4	CN(C)CCN(C)CCCOc1ccc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)cc1Cl	10.1016/j.bmcl.2010.04.017
	CHEMBL1085403	7128	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	442	9	1	4	5.4	CN(C)CCN(C)CCCOc1ccc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)cc1Cl	10.1016/j.bmcl.2010.04.017
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	71681803	89587	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)ncn1	10.1021/jm301886t
	CHEMBL2375363	89587	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)ncn1	10.1021/jm301886t
	11672774	4030	11	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	1245	4030	11	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	CHEMBL206548	4030	11	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	11564628	75502	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	193	5	1	2	1.8	c1nc(CCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL204360	75502	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	193	5	1	2	1.8	c1nc(CCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	11629931	76282	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	5	1	2	2.6	c1nc(CCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL205868	76282	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	5	1	2	2.6	c1nc(CCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	70686222	75652	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	2	2	2	1.7	CN[C@@H]1CCN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047464	75652	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	2	2	2	1.7	CN[C@@H]1CCN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	135398737	955	93	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	38	955	93	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	722	955	93	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	CHEMBL42	955	93	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	DB00363	955	93	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	44395314	123660	0	None	-3	2	Human	5.3	pKi	=	5.3	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	2	2	2	3.2	CN1CCC(C(O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL362163	123660	0	None	-3	2	Human	5.3	pKi	=	5.3	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	2	2	2	3.2	CN1CCC(C(O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	126688	403	33	None	1	3	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	4025	403	33	None	1	3	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	CHEMBL293762	403	33	None	1	3	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	66572110	75614	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047269	75614	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	70684085	75645	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	325	1	1	2	3.0	CN1CCCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047457	75645	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	325	1	1	2	3.0	CN1CCCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10063835	63093	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774601	63093	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789356	63093	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	206876	18913	7	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1039/C2MD20212C
	CHEMBL128637	18913	7	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1039/C2MD20212C
	57395620	68187	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915534	68187	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	57395620	68187	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915534	68187	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	90645160	112160	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3c(F)cccc3F)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290587	112160	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3c(F)cccc3F)n2)CC1	10.1016/j.ejmech.2014.06.032
	57390145	69877	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938970	69877	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	49865668	16019	0	None	-51	4	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	390	8	1	5	4.3	COc1ccccc1N1CCN(CCCCNc2nccc3ccccc23)CC1	10.1016/j.ejmech.2016.09.074
	CHEMBL1223748	16019	0	None	-51	4	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	390	8	1	5	4.3	COc1ccccc1N1CCN(CCCCNc2nccc3ccccc23)CC1	10.1016/j.ejmech.2016.09.074
	54586764	61508	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N(C)C(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770978	61508	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N(C)C(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	44546966	117085	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	4	1	4	1.9	CC(C)CNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393525	117085	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	4	1	4	1.9	CC(C)CNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	9964223	205664	11	None	-36	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL82244	205664	11	None	-36	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	90645148	112148	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290575	112148	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	56950378	69839	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938845	69839	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	25071940	187330	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	187330	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	90645145	112151	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL3290578	112151	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	66741667	110111	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236566	110111	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	122194623	123993	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	296	3	1	6	1.4	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629826	123993	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	296	3	1	6	1.4	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645145	112151	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290578	112151	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10083861	15475	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)nc2c1	10.1021/jm0502081
	CHEMBL121679	15475	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)nc2c1	10.1021/jm0502081
	121238067	167087	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4290148	167087	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	57394636	68017	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	284	1	0	5	2.6	CN1CCN(c2ncnc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914534	68017	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	284	1	0	5	2.6	CN1CCN(c2ncnc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	71525587	89698	0	None	177	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	255	2	2	7	-0.1	CNC1CN(c2nc(N)nc3cc(C#N)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376802	89698	0	None	177	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	255	2	2	7	-0.1	CNC1CN(c2nc(N)nc3cc(C#N)cnc23)C1	10.1016/j.bmcl.2013.02.091
	753256	179326	12	None	2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL473662	179326	12	None	2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmcl.2012.10.139
	46228143	200060	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595880	200060	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	24745335	294	12	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	294	12	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	294	12	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	294	12	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	24745335	294	12	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	294	12	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	294	12	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	294	12	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	51354583	58869	0	None	2	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.1	c1nc(CCCn2cc(CC3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688975	58869	0	None	2	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.1	c1nc(CCCn2cc(CC3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	71518860	86467	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	2	2	5	2.0	CN1CCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314773	86467	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	2	2	5	2.0	CN1CCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	46228113	202050	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	328	5	2	7	1.4	COc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL608717	202050	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	328	5	2	7	1.4	COc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	164611807	184960	0	None	-162	4	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4854278	184960	0	None	-162	4	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	49841718	69706	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)cc1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935569	69706	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)cc1	10.1016/j.bmcl.2011.10.104
	58227143	117101	1	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393541	117101	1	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	67087450	117110	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	117110	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	46890071	6877	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.7	Cc1cc(OCCCNCC2CCCN(C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084354	6877	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.7	Cc1cc(OCCCNCC2CCCN(C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740470	109771	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3233048	109771	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	2306318	86979	12	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL232421	86979	12	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	121238068	157186	0	None	-281	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	1	6	2.0	CN1CCN(c2nc(N)nc(Cc3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4076046	157186	0	None	-281	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	1	6	2.0	CN1CCN(c2nc(N)nc(Cc3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178341	186785	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489271	186785	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178341	186785	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489271	186785	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25178556	189061	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL509961	189061	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	23649429	96268	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	2	0	5	2.7	COc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL261224	96268	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	2	0	5	2.7	COc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	44478385	5744	6	None	9	4	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078642	5744	6	None	9	4	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	25178344	186862	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489664	186862	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	24853597	96267	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1cccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)c1	10.1021/jm7014217
	CHEMBL261223	96267	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1cccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)c1	10.1021/jm7014217
	8070044	155928	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	300	4	0	5	2.4	CC(C)COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL406123	155928	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	300	4	0	5	2.4	CC(C)COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	16061844	61511	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(NCc3ccccc3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770981	61511	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(NCc3ccccc3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	57403351	68294	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2cc(NCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916499	68294	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2cc(NCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	156011443	178317	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4636487	178317	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651225	178317	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25178339	186759	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489061	186759	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354585	58871	0	None	-5	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	245	5	1	4	2.0	c1nc(CCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688977	58871	0	None	-5	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	245	5	1	4	2.0	c1nc(CCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	51354876	60170	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1739924	60170	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL3216267	60170	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	12741697	96131	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL260565	96131	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	25178339	186759	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489061	186759	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	919019	136707	7	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2[nH]c3c(N4CCN(C)CC4)ncnc3c2c1	10.1039/C5MD00022J
	CHEMBL3740837	136707	7	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2[nH]c3c(N4CCN(C)CC4)ncnc3c2c1	10.1039/C5MD00022J
	929854	199062	8	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	289	4	2	4	2.7	CN(C)CCNc1ncnc2c1[nH]c1ccc(Cl)cc12	10.1039/C5MD00022J
	CHEMBL588176	199062	8	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	289	4	2	4	2.7	CN(C)CCNc1ncnc2c1[nH]c1ccc(Cl)cc12	10.1039/C5MD00022J
	168278543	192827	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184846	192827	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221764	192827	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168295803	193021	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207206	193021	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222937	193021	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	57402471	67847	0	None	-549	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	8	1	5	4.0	COc1ccc(CN(CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910378	67847	0	None	-549	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	8	1	5	4.0	COc1ccc(CN(CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	164614645	185108	0	None	-57	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	CHEMBL4856572	185108	0	None	-57	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	57394627	68292	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	5	2	5	2.4	CN1CC[C@H](Nc2cc(NCCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916497	68292	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	5	2	5	2.4	CN1CC[C@H](Nc2cc(NCCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	71519179	86457	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	3	4	5	2.1	Nc1nc(N[C@H]2CCNC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314762	86457	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	3	4	5	2.1	Nc1nc(N[C@H]2CCNC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	1204	1919	119	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1919	119	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1919	119	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1919	119	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1919	119	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1919	119	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1278	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1278	2137	84	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2137	84	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2137	84	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2137	84	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	44538833	69690	26	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@H]2CCCC[C@H]2O1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935450	69690	26	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@H]2CCCC[C@H]2O1	10.1016/j.bmcl.2011.10.104
	49841719	69708	0	None	38	3	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69708	0	None	38	3	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	11846300	80237	3	None	-2	4	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL213912	80237	3	None	-2	4	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2011.10.104
	11301441	96125	10	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260549	96125	10	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1016/j.bmcl.2011.10.104
	54582865	61489	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770959	61489	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	54584825	61520	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770990	61520	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25130912	188715	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL505061	188715	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	54584825	61520	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL1770990	61520	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	58227157	115891	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354815	115891	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	58227200	117095	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393535	117095	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	58227337	117113	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393553	117113	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	24745335	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	294	12	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	11957573	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1250	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3692	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19439	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	44595856	89628	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375757	89628	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	1278	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	1279	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	4908365	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	CHEMBL129198	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	11957573	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	1250	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	3692	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL19439	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	25129523	187263	45	None	1	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL492884	187263	45	None	1	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.06.123
	1278	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2137	84	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	11066922	19391	1	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129436	19391	1	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573458	75635	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047447	75635	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573459	75636	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047448	75636	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	9860842	75637	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)cc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047449	75637	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)cc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	11957573	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	1250	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	3692	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL19439	1997	34	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	71525681	89699	0	None	2	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89699	0	None	2	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	136042270	69886	0	None	426	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938979	69886	0	None	426	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	25178552	173387	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452848	173387	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178552	173387	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL452848	173387	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	90645158	112158	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(Cl)cc1-c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290585	112158	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(Cl)cc1-c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2014.06.032
	57399828	68298	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	345	5	1	5	3.8	CC(C)(C)CCNc1cc(N2CCN(C3CCCCC3)CC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916502	68298	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	345	5	1	5	3.8	CC(C)(C)CCNc1cc(N2CCN(C3CCCCC3)CC2)ncn1	10.1016/j.bmcl.2011.07.114
	805994	174331	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL455133	174331	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm800876b
	805994	174331	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL455133	174331	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm901379s
	168286141	192900	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5192993	192900	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222231	192900	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	46890167	7226	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN1CCC(N(C)CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085872	7226	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN1CCC(N(C)CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	9966927	18843	5	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128252	18843	5	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9966927	18843	5	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128252	18843	5	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0341047
	9966927	18843	5	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128252	18843	5	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	12686479	161771	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1021/jm7014217
	CHEMBL414183	161771	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1021/jm7014217
	1265	939	60	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	6422124	939	60	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	CHEMBL14638	939	60	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	1265	939	60	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	6422124	939	60	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	CHEMBL14638	939	60	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	57390262	68156	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	290	1	2	7	1.2	Nc1nc(N2CC(N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915050	68156	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	290	1	2	7	1.2	Nc1nc(N2CC(N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	155552102	176539	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4576393	176539	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597352	176539	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	25178936	186758	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489060	186758	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178936	186758	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489060	186758	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	137646103	157882	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	393	8	3	3	3.7	O=C(O)C(Cc1cccc2ccccc12)NC(=O)[C@@H](CS)Cc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4084275	157882	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	393	8	3	3	3.7	O=C(O)C(Cc1cccc2ccccc12)NC(=O)[C@@H](CS)Cc1ccccc1	10.1016/j.ejmech.2016.09.074
	42624149	110113	1	None	-3	3	Mouse	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	110113	1	None	-3	3	Mouse	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	58227127	117103	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393543	117103	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	1204	1919	119	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	44395308	66798	0	None	39	2	Human	7.2	pKi	=	7.2	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	369	1	1	2	2.2	CN1CCN(C(=O)c2cc3cc(I)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186108	66798	0	None	39	2	Human	7.2	pKi	=	7.2	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	369	1	1	2	2.2	CN1CCN(C(=O)c2cc3cc(I)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	155552061	176454	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	176454	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	176454	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10017821	103759	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL308913	103759	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1919	119	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10023327	168992	0	None	-144	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168992	0	None	-144	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11499454	74391	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	6	2	2	2.5	CC(C)(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL202638	74391	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	6	2	2	2.5	CC(C)(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	10446240	74969	1	None	-50	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	277	8	2	2	3.6	Clc1ccc(CNCCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	CHEMBL203263	74969	1	None	-50	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	277	8	2	2	3.6	Clc1ccc(CNCCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	11622495	76548	0	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206303	76548	0	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	1267	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	3035905	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	CHEMBL260374	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	12686479	161771	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL414183	161771	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1016/j.bmcl.2011.06.123
	25129523	187263	45	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	187263	45	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25129527	187390	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	187390	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	70681962	75655	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.3	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047467	75655	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.3	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCNCC1	10.1016/j.ejmech.2012.06.016
	70688339	75656	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047468	75656	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1267	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	3035905	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	CHEMBL260374	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	1267	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	1267	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	3035905	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	3035905	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	CHEMBL260374	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	CHEMBL260374	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	24854635	96313	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	310	1	0	4	3.0	Cc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL261519	96313	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	310	1	0	4	3.0	Cc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	10085528	195171	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202330	195171	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL550570	195171	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10041484	195227	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	316	9	3	3	3.6	N=C(NCCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL551047	195227	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	316	9	3	3	3.6	N=C(NCCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1267	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1252	2004	21	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	9793868	2004	21	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL417096	2004	21	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	71681641	89635	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	350	8	4	8	2.2	CC(C)CCc1cc(NCCNc2cc(Cl)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375764	89635	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	350	8	4	8	2.2	CC(C)CCc1cc(NCCNc2cc(Cl)nc(N)n2)nc(N)n1	10.1021/jm301886t
	11492189	75977	0	None	-10	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	1	2	2.5	CCN(CC)CCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205511	75977	0	None	-10	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	1	2	2.5	CCN(CC)CCCCCc1c[nH]cn1	10.1021/jm0504353
	14712103	61855	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	201	5	2	2	1.7	c1ccc(CNCCc2c[nH]cn2)cc1	10.1016/j.bmcl.2011.06.123
	CHEMBL17740	61855	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	201	5	2	2	1.7	c1ccc(CNCCc2c[nH]cn2)cc1	10.1016/j.bmcl.2011.06.123
	12741697	96131	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL260565	96131	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.06.123
	9793921	65098	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	164	2	1	1	2.5	c1nc(CC2CCCCC2)c[nH]1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824228	65098	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	164	2	1	1	2.5	c1nc(CC2CCCCC2)c[nH]1	10.1016/j.bmcl.2011.06.123
	24764570	75632	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	2	1.9	CN[C@@H]1CCN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047444	75632	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	2	1.9	CN[C@@H]1CCN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	25178938	186735	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm901379s
	CHEMBL488885	186735	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm901379s
	933006	136548	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2c(c1)[nH]c1c(N3CCN(C)CC3)ncnc12	10.1039/C5MD00022J
	CHEMBL3739430	136548	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2c(c1)[nH]c1c(N3CCN(C)CC3)ncnc12	10.1039/C5MD00022J
	10419556	5326	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106913	5326	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	168273549	192779	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174342	192779	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221438	192779	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	66572103	75606	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cnccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047261	75606	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cnccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	164627020	186384	0	None	-144	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	422	7	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4876025	186384	0	None	-144	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	422	7	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	10515993	5929	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079918	5929	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL154944	5929	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	54587792	61524	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.2	Nc1nc(N2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770994	61524	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.2	Nc1nc(N2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1016/j.bmcl.2011.03.017
	90645156	112156	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	313	3	1	7	0.9	CN1CCN(c2nc(N)nc(-c3ccc(N(C)C)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290583	112156	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	313	3	1	7	0.9	CN1CCN(c2nc(N)nc(-c3ccc(N(C)C)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	763018	136709	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	6	1.3	Cc1nnc2c(N3CCN(C)CC3)nc3ccccc3n12	10.1039/C5MD00022J
	CHEMBL3740846	136709	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	6	1.3	Cc1nnc2c(N3CCN(C)CC3)nc3ccccc3n12	10.1039/C5MD00022J
	44414498	79967	0	None	-173	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	7	0	2	6.9	CN(Cc1ccc(-c2ccc(CN(C)C3CCCCC3)cc2)cc1)C1CCCCC1	10.1016/j.bmc.2008.10.029
	CHEMBL212775	79967	0	None	-173	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	7	0	2	6.9	CN(Cc1ccc(-c2ccc(CN(C)C3CCCCC3)cc2)cc1)C1CCCCC1	10.1016/j.bmc.2008.10.029
	44414433	139504	0	None	-8	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	352	13	2	2	5.9	CCCCCNCc1ccc(-c2ccc(CNCCCCC)cc2)cc1	10.1016/j.bmc.2008.10.029
	CHEMBL379545	139504	0	None	-8	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	352	13	2	2	5.9	CCCCCNCc1ccc(-c2ccc(CNCCCCC)cc2)cc1	10.1016/j.bmc.2008.10.029
	10419556	5326	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106913	5326	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	164623155	185551	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	352	3	4	4	3.4	N/C(=N\C(=O)Nc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4863393	185551	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	352	3	4	4	3.4	N/C(=N\C(=O)Nc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	57395260	69700	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2nc(N)nc(N3CCN(C)CC3)c2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935563	69700	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2nc(N)nc(N3CCN(C)CC3)c2c1	10.1016/j.bmcl.2011.10.104
	11408069	84235	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	CHEMBL221211	84235	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	11408069	84235	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL221211	84235	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	24825995	68116	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914795	68116	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	1267	3775	49	None	-21	28	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-21	28	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-21	28	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	762810	199884	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	262	2	0	5	2.0	CCc1cc2c(N3CCN(C)CC3)ncnc2s1	10.1016/j.bmc.2009.08.059
	CHEMBL594730	199884	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	262	2	0	5	2.0	CCc1cc2c(N3CCN(C)CC3)ncnc2s1	10.1016/j.bmc.2009.08.059
	51354299	58858	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	247	8	1	4	2.4	CCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688945	58858	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	247	8	1	4	2.4	CCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	136773074	157341	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4077914	157341	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	57400473	69698	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935561	69698	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)c1	10.1016/j.bmcl.2011.10.104
	57397301	67851	0	None	-891	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	560	11	1	6	4.6	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910382	67851	0	None	-891	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	560	11	1	6	4.6	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	127026018	137467	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753985	137467	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	73346048	91838	0	None	-15	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	7	2	3	3.0	O=C(NCCc1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL2413359	91838	0	None	-15	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	7	2	3	3.0	O=C(NCCc1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164617783	184566	0	None	-104	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	8	4	4	2.8	CCC(CNC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848815	184566	0	None	-104	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	8	4	4	2.8	CCC(CNC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	71519499	86463	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	1	6	2.1	CN1CCN(c2nc(N)nc3c2cc(-c2ccc(F)cc2)n3C)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314768	86463	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	1	6	2.1	CN1CCN(c2nc(N)nc3c2cc(-c2ccc(F)cc2)n3C)CC1	10.1016/j.bmcl.2012.10.139
	54583872	61490	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770960	61490	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)n2)C1	10.1016/j.bmcl.2011.03.017
	1278	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	1279	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	4908365	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	CHEMBL129198	2137	84	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	58227388	117098	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393538	117098	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	155517880	176643	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	155517880	176643	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176643	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176643	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	46890626	6556	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@@H]3CCCN[C@@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083097	6556	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@@H]3CCCN[C@@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	25178938	186735	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm800876b
	CHEMBL488885	186735	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm800876b
	9812843	205221	1	None	-4	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78838	205221	1	None	-4	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	647957	29046	13	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	2	0	6	0.4	Cc1cc(N2CCN(C)CC2)nc(N2CCOCC2)n1	10.1039/C5MD00022J
	CHEMBL1381010	29046	13	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	2	0	6	0.4	Cc1cc(N2CCN(C)CC2)nc(N2CCOCC2)n1	10.1039/C5MD00022J
	747187	136613	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	249	4	1	3	2.8	Cc1cc2nc(SCCN(C)C)[nH]c2cc1C	10.1039/C5MD00022J
	CHEMBL3739949	136613	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	249	4	1	3	2.8	Cc1cc2nc(SCCN(C)C)[nH]c2cc1C	10.1039/C5MD00022J
	168268716	192738	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5170276	192738	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221176	192738	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168268853	192761	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5180504	192761	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221353	192761	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	5013	100209	32	None	-16	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2ccc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL288591	100209	32	None	-16	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2ccc3ccccc3n2)CC1	10.1021/jm7014217
	10243990	5862	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079552	5862	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL155830	5862	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	11065080	19402	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1007/s00044-004-0106-7
	CHEMBL129488	19402	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1007/s00044-004-0106-7
	11065080	19402	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0341047
	CHEMBL129488	19402	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0341047
	117724523	117089	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393529	117089	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	11594278	14658	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL120591	14658	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	11065080	19402	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL129488	19402	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	44462956	5567	0	None	-2	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL1077276	5567	0	None	-2	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	71603140	89690	0	None	97	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	3	2	5	1.5	CNC1CN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376794	89690	0	None	97	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	3	2	5	1.5	CNC1CN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25147772	173385	9	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	173385	9	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25147772	173385	9	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL452847	173385	9	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	44129131	199431	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	440	8	1	7	2.1	CCN(CC)S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591500	199431	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	440	8	1	7	2.1	CCN(CC)S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	25071319	189038	14	None	9	2	Rat	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	189038	14	None	9	2	Rat	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	16082828	80231	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213886	80231	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	57398091	68079	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	3.0	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914757	68079	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	3.0	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	57401517	68096	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)c(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914774	68096	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)c(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	3035746	188156	9	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL1237146	188156	9	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL498770	188156	9	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	136030655	56452	0	None	2	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56452	0	None	2	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136192874	56453	0	None	1	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56453	0	None	1	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1204	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1247	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1375	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	774	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1204	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	53321919	56448	0	None	-3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632409	56448	0	None	-3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10060213	102884	1	None	3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL306077	102884	1	None	3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	155491015	176471	4	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	176471	4	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	176471	4	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	44609412	89645	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	322	6	4	8	1.6	Nc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375773	89645	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	322	6	4	8	1.6	Nc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	25129523	187263	45	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm301886t
	CHEMBL492884	187263	45	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm301886t
	51354584	58870	0	None	11	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	6	1	4	2.4	c1nc(CCCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688976	58870	0	None	11	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	6	1	4	2.4	c1nc(CCCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	76314798	106025	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	314	3	1	7	1.4	CN1CCN(c2cc(-c3ccc([N+](=O)[O-])cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL3133131	106025	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	314	3	1	7	1.4	CN1CCN(c2cc(-c3ccc([N+](=O)[O-])cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	136042269	69885	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938978	69885	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	71525678	89696	0	None	-7	6	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89696	0	None	-7	6	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25178937	186760	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL489062	186760	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25071319	189038	14	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	189038	14	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	44129209	202006	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	586	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccc(I)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL608373	202006	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	586	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccc(I)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11013044	18878	1	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	333	4	1	2	3.2	O=C(c1cc2ccccc2[nH]1)N1CCN(CCc2ccccc2)CC1	10.1021/jm0341047
	CHEMBL128470	18878	1	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	333	4	1	2	3.2	O=C(c1cc2ccccc2[nH]1)N1CCN(CCc2ccccc2)CC1	10.1021/jm0341047
	122194625	123995	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629828	123995	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	52952957	63133	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774594	63133	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789799	63133	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	24854703	166863	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	364	5	0	6	3.0	COc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL428606	166863	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	364	5	0	6	3.0	COc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL4748908	214012	1	None	-8	12	Human	6.2	pKi	=	6.2	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL		None	None	None		CN1CCN(c2ccnc(NCCc3ccccc3)n2)CC1	10.6019/CHEMBL4800732
	156013005	177475	0	None	-2	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	899	20	2	7	10.6	Cc1cc(OCCCNC(=O)CCCCCN2/C(=C\C=C\C=C\C3=[N+](CCCCS(=O)(=O)[O-])c4ccccc4C3(C)C)C(C)(C)c3ccccc32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4639472	177475	0	None	-2	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	899	20	2	7	10.6	Cc1cc(OCCCNC(=O)CCCCCN2/C(=C\C=C\C=C\C3=[N+](CCCCS(=O)(=O)[O-])c4ccccc4C3(C)C)C(C)(C)c3ccccc32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	168268827	192755	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5179906	192755	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221314	192755	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	52952957	63133	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774594	63133	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789799	63133	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	25071319	189038	14	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	189038	14	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	46228101	199775	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594022	199775	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL4748908	214012	1	None	-8	12	Human	6.2	pKi	=	6.2	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL		None	None	None		CN1CCN(c2ccnc(NCCc3ccccc3)n2)CC1	10.6019/CHEMBL4800732
	46228083	199718	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	374	5	2	6	3.1	CN1CCN(c2cc(NCc3ccc(-c4ccccc4)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593551	199718	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	374	5	2	6	3.1	CN1CCN(c2cc(NCc3ccc(-c4ccccc4)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890549	6553	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083085	6553	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	112936352	137469	1	None	-17	2	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2cc(-c3ccccc3)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3754008	137469	1	None	-17	2	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2cc(-c3ccccc3)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	155563489	175305	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.7	CCC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4573929	175305	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.7	CCC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71518865	86433	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	333	2	2	6	1.8	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(C#N)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314494	86433	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	333	2	2	6	1.8	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(C#N)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	57401167	69840	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938846	69840	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24826679	68073	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914751	68073	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	168294161	192982	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204739	192982	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222742	192982	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	168294770	192996	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208327	192996	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222806	192996	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	44450438	96092	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc3ccccc3nc2NCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260371	96092	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc3ccccc3nc2NCc2ccccc2)CC1	10.1021/jm7014217
	71528209	86943	0	None	-1148	7	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	465	6	0	3	5.8	CC1=CC(C)=[N+]2C1=C(c1ccc(OCCCN3CCCCC3)cc1)c1c(C)cc(C)n1[B-]2(F)F	10.1021/ml300383n
	CHEMBL2323582	86943	0	None	-1148	7	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	465	6	0	3	5.8	CC1=CC(C)=[N+]2C1=C(c1ccc(OCCCN3CCCCC3)cc1)c1c(C)cc(C)n1[B-]2(F)F	10.1021/ml300383n
	121238069	159102	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting methodDisplacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.04.033
	CHEMBL4097856	159102	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting methodDisplacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.04.033
	24826268	68022	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	3	2	6	2.8	CCN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914544	68022	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	3	2	6	2.8	CCN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	56851443	71599	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923024	71599	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963459	71599	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	121238069	159102	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4097856	159102	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178132	186915	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL490073	186915	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25178132	186915	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL490073	186915	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	168283321	192871	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183015	192871	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222057	192871	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	24759622	169655	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL443764	169655	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm800876b
	24759622	169655	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm7014217
	24759622	169655	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL443764	169655	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL443764	169655	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm901379s
	164622259	185706	0	None	-7244	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	336	6	4	5	1.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccco1	10.1016/j.ejmech.2021.113190
	CHEMBL4865965	185706	0	None	-7244	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	336	6	4	5	1.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccco1	10.1016/j.ejmech.2021.113190
	24826167	68117	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914796	68117	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	54581938	61502	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770972	61502	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	46890019	7280	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7280	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	24994945	110112	1	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CN[C@@H]1CCN(c2cc(C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236567	110112	1	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CN[C@@H]1CCN(c2cc(C)nc(N)n2)C1	10.1021/jm401727m
	1223	950	41	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	950	41	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	950	41	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	51354301	58862	0	None	-10	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688969	58862	0	None	-10	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	164609017	184401	0	None	-13	6	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	10	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4846201	184401	0	None	-13	6	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	10	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCCc1ccccc1	10.1016/j.ejmech.2021.113190
	168294166	192990	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209081	192990	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222775	192990	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	168294178	193011	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5205197	193011	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222887	193011	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	127026019	137435	0	None	-6	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	378	2	0	6	1.7	CN1CCN(c2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753705	137435	0	None	-6	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	378	2	0	6	1.7	CN1CCN(c2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	11546127	95455	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL25725	95455	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	10715823	5898	0	None	-20	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	281	10	2	3	3.3	CCCCC(CC)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079747	5898	0	None	-20	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	281	10	2	3	3.3	CCCCC(CC)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	1267	3775	49	None	-33	28	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3775	49	None	-33	28	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3775	49	None	-33	28	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	25071319	189038	14	None	-9	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.06.123
	CHEMBL509540	189038	14	None	-9	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.06.123
	25109291	191362	10	None	-501	8	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1016/j.bmcl.2011.06.123
	CHEMBL519240	191362	10	None	-501	8	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1016/j.bmcl.2011.06.123
	1204	1919	119	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1919	119	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1919	119	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1919	119	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1919	119	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1919	119	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10446295	2119	34	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	1277	2119	34	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL185951	2119	34	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	1267	3775	49	None	-21	28	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3775	49	None	-21	28	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3775	49	None	-21	28	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	937120	175077	8	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	244	1	0	3	1.8	CN1CCN(C(=O)c2cc3ccccc3o2)CC1	10.1016/j.bmc.2020.115924
	CHEMBL4568949	175077	8	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	244	1	0	3	1.8	CN1CCN(C(=O)c2cc3ccccc3o2)CC1	10.1016/j.bmc.2020.115924
	71681974	89620	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1cc(-c2ccccc2)nc(N)n1)c1ccnc(N)n1	10.1021/jm301886t
	CHEMBL2375749	89620	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1cc(-c2ccccc2)nc(N)n1)c1ccnc(N)n1	10.1021/jm301886t
	71681475	89626	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	317	8	5	9	1.0	CC(C)CNc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375755	89626	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	317	8	5	9	1.0	CC(C)CNc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681643	89637	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375766	89637	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	71681645	89639	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375768	89639	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	11679993	75988	0	None	-25	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.0	CC(C)CNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205559	75988	0	None	-25	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.0	CC(C)CNCCCCc1c[nH]cn1	10.1021/jm0504353
	11087453	75615	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047270	75615	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572113	75620	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3ccc(N)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047275	75620	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3ccc(N)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573457	75634	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	2	1	2	2.4	Cc1cccc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047446	75634	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	2	1	2	2.4	Cc1cccc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70688340	75664	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3c(F)ccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047476	75664	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3c(F)ccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	51354875	58878	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688986	58878	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25178937	186760	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL489062	186760	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	11846635	78651	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	CHEMBL211242	78651	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	4060730	19347	56	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19347	56	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	90645159	112159	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	2.2	CN1CCN(c2nc(N)nc(-c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290586	112159	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	2.2	CN1CCN(c2nc(N)nc(-c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	162671115	182923	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	266	2	0	3	1.9	CN1CCN(CC2Cc3cc(Cl)ccc3O2)CC1	10.1016/j.bmc.2020.115924
	CHEMBL4792753	182923	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	266	2	0	3	1.9	CN1CCN(CC2Cc3cc(Cl)ccc3O2)CC1	10.1016/j.bmc.2020.115924
	10197870	5269	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106612	5269	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	91613	17955	34	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL12620	17955	34	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	71681474	89625	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	246	5	4	8	-0.0	Nc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375754	89625	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	246	5	4	8	-0.0	Nc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	11637064	74925	0	None	-25	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	1	2	2.2	c1nc(CCCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203228	74925	0	None	-25	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	1	2	2.2	c1nc(CCCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	11708125	76490	3	None	-251	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	5	1	2	1.3	CN(C)CCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206000	76490	3	None	-251	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	5	1	2	1.3	CN(C)CCCCc1c[nH]cn1	10.1021/jm0504353
	11499376	138478	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	5	2	2	2.1	CC(C)(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL377491	138478	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	5	2	2	2.1	CC(C)(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	56672434	65094	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	1	2	3.3	CN1CCC(=Cc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824048	65094	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	1	2	3.3	CN1CCC(=Cc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2011.06.123
	66572109	75612	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3c([N+](=O)[O-])cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047267	75612	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3c([N+](=O)[O-])cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10957150	75629	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cccc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047441	75629	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cccc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	3939214	126619	6	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	262	1	1	1	3.4	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCCC1	10.1016/j.bmcl.2004.08.035
	CHEMBL365257	126619	6	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	262	1	1	1	3.4	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCCC1	10.1016/j.bmcl.2004.08.035
	11086114	7362	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL108649	7362	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	2247	504	81	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	249	504	81	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	2603	504	81	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	CHEMBL296419	504	81	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	DB00637	504	81	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	44417471	12739	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187948	12739	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	CHEMBL534496	12739	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	164614841	185250	0	None	-223	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	7	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	CHEMBL4858849	185250	0	None	-223	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	7	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	9923162	18908	6	None	199	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128613	18908	6	None	199	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10967174	19410	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129540	19410	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	1204	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	9923162	18908	6	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128613	18908	6	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0341047
	10967174	19410	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19410	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	1278	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	54580746	61361	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.1	Nc1nc(N2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770160	61361	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.1	Nc1nc(N2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2011.03.017
	1278	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1279	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	4908365	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL129198	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	58227157	115891	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354815	115891	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	58227179	117108	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393548	117108	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	58227259	117116	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	117116	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	42624150	110122	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236577	110122	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	24827129	68106	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2c(Cl)cc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914785	68106	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2c(Cl)cc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	9923162	18908	6	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128613	18908	6	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	10967174	19410	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129540	19410	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	44129212	199430	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.3	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(C(F)(F)F)cccc3n2)CC1	10.1021/jm901379s
	CHEMBL591496	199430	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.3	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(C(F)(F)F)cccc3n2)CC1	10.1021/jm901379s
	24825522	187619	0	None	-6	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	283	3	1	3	3.3	CC1CC(C)(C)N=C(c2cccc(Cc3c[nH]cn3)c2)O1	10.1021/jm7014149
	CHEMBL494982	187619	0	None	-6	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	283	3	1	3	3.3	CC1CC(C)(C)N=C(c2cccc(Cc3c[nH]cn3)c2)O1	10.1021/jm7014149
	51354300	58859	0	None	15	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688946	58859	0	None	15	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	58227373	176484	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	176484	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	176484	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	168294182	193024	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208722	193024	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222960	193024	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	46890546	6483	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6483	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228069	200127	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	3	1	7	1.2	CN1CCN(c2nc(N)nc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596348	200127	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	3	1	7	1.2	CN1CCN(c2nc(N)nc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	63868172	176032	2	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	222	2	1	6	-0.2	CCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4590628	176032	2	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	222	2	1	6	-0.2	CCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	164614549	184919	0	None	-575	6	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	340	9	4	4	2.2	CCCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4853668	184919	0	None	-575	6	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	340	9	4	4	2.2	CCCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	164621681	185556	0	None	-64	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	444	7	4	5	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(OC(F)(F)F)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4863523	185556	0	None	-64	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	444	7	4	5	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(OC(F)(F)F)cc1	10.1016/j.ejmech.2021.113190
	164627241	186405	0	None	-269	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	6	4	4	3.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Br)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4876267	186405	0	None	-269	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	6	4	4	3.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Br)cc1	10.1016/j.ejmech.2021.113190
	11086114	7362	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL108649	7362	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	71519347	86460	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	274	4	2	6	1.2	CCCCn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314765	86460	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	274	4	2	6	1.2	CCCCn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	54754351	68188	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915535	68188	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	54754351	68188	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915535	68188	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	58227242	117112	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2cnnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393552	117112	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2cnnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	121238067	167087	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2017.09.035
	CHEMBL4290148	167087	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2017.09.035
	57400778	68181	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915346	68181	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	54584827	61526	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770996	61526	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	57400778	68181	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915346	68181	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400589	69879	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)cnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938972	69879	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)cnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	90645164	112164	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1C	10.1016/j.ejmech.2014.06.032
	CHEMBL3290591	112164	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1C	10.1016/j.ejmech.2014.06.032
	57399016	68147	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915040	68147	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	46890548	6552	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083084	6552	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46929551	16712	0	None	-52	2	Human	6.1	pKi	=	6.1	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2ccccn2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243335	16712	0	None	-52	2	Human	6.1	pKi	=	6.1	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2ccccn2)c2c[nH]cn2)cc1	10.1021/jm100643t
	5159263	10351	11	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	259	4	3	4	0.8	Cc1cc(C)nc(N/C(N)=N/CCc2c[nH]cn2)n1	10.1021/jm7014777
	CHEMBL1162351	10351	11	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	259	4	3	4	0.8	Cc1cc(C)nc(N/C(N)=N/CCc2c[nH]cn2)n1	10.1021/jm7014777
	71525679	89697	0	None	-6	6	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89697	0	None	-6	6	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25178130	189721	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL515870	189721	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	25178130	189721	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL515870	189721	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	164622153	185544	0	None	-181	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	6	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Cl)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4863347	185544	0	None	-181	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	6	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Cl)cc1	10.1016/j.ejmech.2021.113190
	54581939	61514	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770984	61514	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	122193095	123888	0	None	-3	2	Mouse	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assayDisplacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123888	0	None	-3	2	Mouse	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assayDisplacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	58227260	117106	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393546	117106	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	57398176	68299	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	273	2	0	5	1.4	CN1CCN(c2cc(N3[C@@H]4CCC[C@@H]3C4)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916503	68299	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	273	2	0	5	1.4	CN1CCN(c2cc(N3[C@@H]4CCC[C@@H]3C4)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	127050776	140222	0	None	6	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	140222	0	None	6	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	164627189	186297	0	None	-831	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	298	6	4	4	1.0	CCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4874758	186297	0	None	-831	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	298	6	4	4	1.0	CCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	71519015	86439	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	246	1	2	5	0.7	CN1CCCN(c2nc(N)nc3[nH]ccc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314500	86439	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	246	1	2	5	0.7	CN1CCCN(c2nc(N)nc3[nH]ccc23)CC1	10.1016/j.bmcl.2012.10.139
	57400737	68149	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Cl)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915042	68149	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Cl)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	57395494	68143	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915032	68143	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	57395499	68152	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	2	7	1.8	C[C@H]1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CCN1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915046	68152	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	2	7	1.8	C[C@H]1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CCN1	10.1016/j.bmcl.2011.08.014
	46890677	7259	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086038	7259	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	56851612	71572	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923032	71572	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963245	71572	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	56851602	71574	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923027	71574	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963260	71574	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	25178345	173422	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccco3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452925	173422	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccco3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354303	58865	0	None	-1	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.7	c1nc(CCCn2cc(C3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688971	58865	0	None	-1	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.7	c1nc(CCCn2cc(C3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25147771	174214	0	None	-1	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454890	174214	0	None	-1	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10656971	16389	4	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL123137	16389	4	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1021/jm7014217
	25179123	189076	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL510181	189076	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	10197870	5269	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106612	5269	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	168273471	192777	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173079	192777	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221433	192777	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	57402472	67850	0	None	-1819	3	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	526	11	1	6	4.0	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910381	67850	0	None	-1819	3	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	526	11	1	6	4.0	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	44576985	10350	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	212	4	7	4	-1.3	CC(/C=C(\C)NNC(=N)N)=N/NC(=N)N	10.1021/jm7014777
	CHEMBL1162347	10350	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	212	4	7	4	-1.3	CC(/C=C(\C)NNC(=N)N)=N/NC(=N)N	10.1021/jm7014777
	71519010	86434	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	386	2	2	5	2.7	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Br)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314495	86434	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	386	2	2	5	2.7	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Br)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	1204	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	1247	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	1375	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	774	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	54587790	61510	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770980	61510	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	54753437	68154	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL1915048	68154	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2014.12.027
	11750684	83431	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	CHEMBL219982	83431	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	54753437	68154	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1021/jm401727m
	CHEMBL1915048	68154	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1021/jm401727m
	24825794	68027	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914549	68027	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	71525679	89697	0	None	6	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89697	0	None	6	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25071940	187330	0	None	5	2	Rat	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	187330	0	None	5	2	Rat	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	71525586	89695	0	None	22	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	2	1	6	0.9	CCc1cnc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376799	89695	0	None	22	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	2	1	6	0.9	CCc1cnc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	44129135	199407	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	454	6	1	8	1.1	CN1CCN(c2nc(NCCS(=O)(=O)N3CCOCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591250	199407	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	454	6	1	8	1.1	CN1CCN(c2nc(NCCS(=O)(=O)N3CCOCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	53378711	110123	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236578	110123	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	54753437	68154	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915048	68154	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	135525668	68183	0	None	263	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915348	68183	0	None	263	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	49841611	69704	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935567	69704	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	1248	1412	28	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	3077	1412	28	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	90045	1412	28	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	CHEMBL12344	1412	28	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	11615753	72909	7	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3sccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL200546	72909	7	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3sccc3[nH]2)CC1	10.1021/jm0502081
	10444761	123812	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3ccsc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL362495	123812	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3ccsc3[nH]2)CC1	10.1021/jm0502081
	16082826	80284	0	None	3	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@H]2C[C@@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214156	80284	0	None	3	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@H]2C[C@@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	122193097	123890	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628044	123890	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	57390344	68192	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	248	5	3	6	0.3	CNC1CN(c2cc(N)nc(NCC3CC3)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915539	68192	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	248	5	3	6	0.3	CNC1CN(c2cc(N)nc(NCC3CC3)n2)C1	10.1016/j.bmcl.2011.07.125
	49841607	69692	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	CHEMBL1935555	69692	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	12686451	204026	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL1743969	204026	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL6992	204026	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	51354010	58854	0	None	-1	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	281	7	1	4	2.6	c1ccc(Cc2cn(CCCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688941	58854	0	None	-1	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	281	7	1	4	2.6	c1ccc(Cc2cn(CCCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	127036979	137463	0	None	1	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753962	137463	0	None	1	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	118720434	115885	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	241	3	2	5	0.6	CN[C@@H]1CCN(c2cc(CCl)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354808	115885	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	241	3	2	5	0.6	CN[C@@H]1CCN(c2cc(CCl)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	11212855	84201	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL220934	84201	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	57401602	68300	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	242	1	0	4	1.3	Cc1ccc(C#N)c(N2CC3CN(C)CC3C2)n1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916504	68300	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	242	1	0	4	1.3	Cc1ccc(C#N)c(N2CC3CN(C)CC3C2)n1	10.1016/j.bmcl.2011.07.114
	156010537	178285	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634235	178285	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651053	178285	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	90654503	110285	0	None	-123	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	483	9	1	4	4.9	O=C1NC(c2ccccc2)(c2ccccc2)C(=O)N1Cc1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	CHEMBL3238447	110285	0	None	-123	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	483	9	1	4	4.9	O=C1NC(c2ccccc2)(c2ccccc2)C(=O)N1Cc1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	57394626	68289	0	None	14	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916494	68289	0	None	14	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	49841723	69697	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)cc1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935560	69697	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)cc1	10.1016/j.bmcl.2011.10.104
	112936251	137241	1	None	1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752085	137241	1	None	1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	25178340	186761	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm800876b
	CHEMBL489069	186761	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm800876b
	25179123	189076	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL510181	189076	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25178340	186761	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm901379s
	CHEMBL489069	186761	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm901379s
	164618690	186132	0	None	-13	5	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	3.4	CCCCCCNC(=O)/N=C(\N)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4872375	186132	0	None	-13	5	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	3.4	CCCCCCNC(=O)/N=C(\N)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	44547126	117087	1	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393527	117087	1	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	118725540	117084	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	1.9	CN[C@@H]1CCN(c2ccnc(N3CCCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393524	117084	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	1.9	CN[C@@H]1CCN(c2ccnc(N3CCCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	46890676	7211	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	1	4	4.0	Cc1cc(OCCCN2CC3CN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085804	7211	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	1	4	4.0	Cc1cc(OCCCN2CC3CN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57393651	69878	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3cc4cccnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938971	69878	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3cc4cccnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24853666	156094	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	1	1	4	1.7	CN1CCCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL406327	156094	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	1	1	4	1.7	CN1CCCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	112936267	137270	1	None	-2	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2cc(-c3ccccc3)nc(NCCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752383	137270	1	None	-2	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2cc(-c3ccccc3)nc(NCCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	127051301	140175	0	None	5	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	140175	0	None	5	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130914	187230	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL492677	187230	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	71519178	86456	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314761	86456	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	127052293	140213	3	None	34	4	Human	7.0	pKi	=	7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	140213	3	None	34	4	Human	7.0	pKi	=	7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1530	2169	50	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	3827	2169	50	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	7206	2169	50	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	CHEMBL534	2169	50	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	DB00920	2169	50	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	124087	1377	114	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	7157	1377	114	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	814	1377	114	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	CHEMBL1172	1377	114	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	DB00967	1377	114	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	71548412	1896	20	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	9879	1896	20	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	CHEMBL4790724	1896	20	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	71548412	1896	20	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	9879	1896	20	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	CHEMBL4790724	1896	20	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	1278	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1279	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	4908365	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	CHEMBL129198	2137	84	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1204	1919	119	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1919	119	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1919	119	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1919	119	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1919	119	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1919	119	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1236	2054	25	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1237	2054	25	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	156615	2054	25	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	CHEMBL268229	2054	25	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1204	1919	119	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1919	119	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1919	119	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1919	119	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1919	119	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1919	119	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1241	2024	52	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1242	2024	52	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	3614	2024	52	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	CHEMBL507	2024	52	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1204	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1204	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1204	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1204	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1204	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1247	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1247	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1247	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1247	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1247	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1375	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1375	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1375	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1375	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1375	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	774	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	774	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	774	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	774	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	774	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	DB05381	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	DB05381	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	DB05381	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	DB05381	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	DB05381	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1919	119	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	None	216115	0	3H-Histamine	4	7	Guinea pig	10.0	pKi	=	10	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	1204	1919	119	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	None	216115	0	3H-Histamine	4	7	Guinea pig	9.8	pKi	=	9.8	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	179	399	115	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	2159	399	115	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	963	399	115	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	CHEMBL243712	399	115	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	DB06288	399	115	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	242	469	124	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	34	469	124	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	60795	469	124	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	CHEMBL1112	469	124	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	DB01238	469	124	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	1231	934	116	3H-Histamine	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	1231	934	116	3H-PYRILAMINE	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	2756	934	116	3H-Histamine	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	2756	934	116	3H-PYRILAMINE	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	645	934	116	3H-Histamine	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	645	934	116	3H-PYRILAMINE	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	CHEMBL30	934	116	3H-Histamine	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	CHEMBL30	934	116	3H-PYRILAMINE	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	DB00501	934	116	3H-Histamine	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	DB00501	934	116	3H-PYRILAMINE	-177	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	277	1289	62	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1289	62	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1289	62	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1289	62	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1289	62	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1248	1412	28	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	3077	1412	28	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	90045	1412	28	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	CHEMBL12344	1412	28	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	1224	1420	83	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	3100	1420	83	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	8980	1420	83	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	916	1420	83	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	CHEMBL657	1420	83	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	DB01075	1420	83	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	1212	1649	50	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	204	1649	50	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	3372	1649	50	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	CHEMBL726	1649	50	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	DB00623	1649	50	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	1353	1898	93	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	3559	1898	93	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	86	1898	93	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	CHEMBL54	1898	93	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	DB00502	1898	93	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	135398745	2893	112	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	47	2893	112	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	CHEMBL715	2893	112	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	DB00334	2893	112	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	119570	3137	96	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	2233	3137	96	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	953	3137	96	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	CHEMBL301265	3137	96	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	DB00413	3137	96	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	2337	3232	77	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	50	3232	77	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	5002	3232	77	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	CHEMBL716	3232	77	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	DB01224	3232	77	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	1234	3266	68	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	3001055	3266	68	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	CHEMBL512	3266	68	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	DB00863	3266	68	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	2389	3306	118	3H-Histamine	-26915	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	5073	3306	118	3H-Histamine	-26915	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	96	3306	118	3H-Histamine	-26915	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	CHEMBL85	3306	118	3H-Histamine	-26915	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	DB00734	3306	118	3H-Histamine	-26915	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	6917970	3665	61	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	8370	3665	61	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	CHEMBL487387	3665	61	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	213	3824	55	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	2717	3824	55	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	5533	3824	55	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	CHEMBL621	3824	55	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	DB00656	3824	55	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	185	3975	60	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	5311271	3975	60	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	CHEMBL74355	3975	60	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	DB16351	3975	60	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	2865	4112	73	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	59	4112	73	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	60854	4112	73	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	CHEMBL708	4112	73	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	DB00246	4112	73	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	10297	27081	30	3H-Histamine	-38	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	C[C@H](N)[C@H](O)c1ccccc1	None
	CHEMBL136560	27081	30	3H-Histamine	-38	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	C[C@H](N)[C@H](O)c1ccccc1	None
	156391	46770	99	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	CHEMBL1200806	46770	99	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	CHEMBL154	46770	99	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	10624	70271	19	3H-Histamine	-776	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	138543650	70271	19	3H-Histamine	-776	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	CHEMBL194378	70271	19	3H-Histamine	-776	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	21830793	91806	10	3H-8-OH-DPAT	-66069	46	Bovine	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	0	8	0.6	COc1ccccc1N1CCN(CCCCn2ncc(=O)n(C)c2=O)CC1	None
	CHEMBL2413154	91806	10	3H-8-OH-DPAT	-66069	46	Bovine	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	0	8	0.6	COc1ccccc1N1CCN(CCCCn2ncc(=O)n(C)c2=O)CC1	None
	46780481	107504	20	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	9903970	107504	20	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	CHEMBL3187365	107504	20	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	CHEMBL3544974	107504	20	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	446220	133496	14	3H-Histamine	-1778	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	303	3	0	5	1.9	COC(=O)[C@H]1[C@@H](OC(=O)c2ccccc2)C[C@@H]2CC[C@H]1N2C	None
	CHEMBL370805	133496	14	3H-Histamine	-1778	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	303	3	0	5	1.9	COC(=O)[C@H]1[C@@H](OC(=O)c2ccccc2)C[C@@H]2CC[C@H]1N2C	None
	4495	196512	92	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	308	5	1	5	2.8	CS(=O)(=O)Nc1ccc([N+](=O)[O-])cc1Oc1ccccc1	None
	CHEMBL56367	196512	92	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	308	5	1	5	2.8	CS(=O)(=O)Nc1ccc([N+](=O)[O-])cc1Oc1ccccc1	None
	54677470	200528	115	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	CHEMBL1256873	200528	115	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	CHEMBL599	200528	115	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	162265	202251	22	3H-Histamine	-239	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	4786	202251	22	3H-Histamine	-239	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	CHEMBL61006	202251	22	3H-Histamine	-239	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	4054	205478	72	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	CHEMBL1699	205478	72	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	CHEMBL807	205478	72	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	11954259	215954	0	3H-Histamine	-33113	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	443	5	0	5	3.5	CN1CCN(CC1)CCC=C2C3=CC=CC=C3SC4=C2C=C(C=C4)S(=O)(=O)N(C)C	None
	None	215968	0	3H-Histamine	-1	28	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	329	2	0	4	3.6	CC1=NC=CN1CC2CCC3=C(C2=O)C4=CC=CC=C4N3C.Cl	None
	104911	215994	0	3H-Histamine	-41686	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	530	7	0	5	5.1	COC1=CC=CC=C1N2CCN(CC2)CCN(C3=CC=CC=N3)C(=O)C4CCCCC4.Cl.Cl.Cl	None
	None	216115	0	3H-PYRILAMINE	-7585	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	25137849	216153	0	3H-Histamine	-4	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	165	3	2	2	1.3	CC(C(C1=CC=CC=C1)O)NC	None
	71290	216153	0	3H-Histamine	-4	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	165	3	2	2	1.3	CC(C(C1=CC=CC=C1)O)NC	None
	None	216285	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	240	7	4	6	-0.8	C(C(C(=O)O)N)SSCC(C(=O)O)N	None
	None	216286	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	149	4	2	3	0.2	CSCCC(C(=O)O)N	None
	None	216287	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	135	3	3	3	-0.3	C(CS)C(C(=O)O)N	None
	None	216288	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	121	2	3	3	-0.7	C(C(C(=O)O)N)S	None
	None	216289	0	3H-Histamine	-1	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	117	0	1	3	-0.0	C1CSC(=O)C1N	None
	None	216290	0	3H-Histamine	-1	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	153	3	3	3	-1.4	C(C(C(=O)O)N)S(=O)O	None
	None	216291	0	3H-Histamine	-1	39	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	169	3	3	4	-1.7	C(C(C(=O)O)N)S(=O)(=O)O	None
	None	216299	0	3H-Histamine	-13	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	149	2	1	2	1.2	CC(C(=O)C1=CC=CC=C1)N	None
	1576	216300	0	3H-Histamine	-16	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	163	3	1	2	1.5	CC(C(=O)C1=CC=CC=C1)NC	None
	135269	216363	0	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	222	5	1	3	2.5	CCCCC(=O)OC1=CC=CC=C1C(=O)O	None
	23681059	216364	0	3H-Histamine	-1	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	CC(C1=CC2=C(C=C1)C=C(C=C2)OC)C(=O)O	None
	123619	216367	0	3H-Histamine	-1412	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	358	3	0	4	4.2	CC1=NC=C(C=C1)C2=NC=C(C=C2C3=CC=C(C=C3)S(=O)(=O)C)Cl	None
	119828	216368	0	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	370	5	1	5	3.5	CCC(=O)NS(=O)(=O)C1=CC=C(C=C1)C2=C(ON=C2C3=CC=CC=C3)C	None
	None	216369	0	3H-Histamine	-7	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	517	8	2	5	5.2	CC1=C(C2=C(N1C(=O)C3=CC=C(C=C3)Cl)C=CC(=C2)OC)CC(=O)NCCC4=CC=C(C=C4)NC(=O)C	None
	None	216462	0	3H-Histamine	-4570	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	347	6	0	3	5.0	CC(=O)N(CC1=CC=CC=C1OC)C2=CC=CC=C2OC3=CC=CC=C3	None
	135409468	2022	69	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	333	2022	69	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	CHEMBL845	2022	69	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	1204	1919	119	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1225	1459	26	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	3958	1459	26	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	667477	1459	26	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	CHEMBL860	1459	26	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	DB01142	1459	26	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	1226	2006	20	3H-Histamine	2	7	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	41376	2006	20	3H-Histamine	2	7	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	CHEMBL12608	2006	20	3H-Histamine	2	7	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	1223	950	41	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	950	41	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	950	41	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1251	2001	39	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	2001	39	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	2001	39	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1253	2045	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	132937	2045	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	CHEMBL19010	2045	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	None	216228	0	3H-Histamine	-13	21	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	None	216228	0	3H-N-alpha-methylhistamine	-13	21	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	1223	950	41	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	950	41	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	950	41	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1267	3775	49	3H-Histamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3775	49	3H-N-alpha-methylhistamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-Histamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-N-alpha-methylhistamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-Histamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-N-alpha-methylhistamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1547484	937	74	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	654	937	74	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	9072	937	74	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	CHEMBL43064	937	74	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	DB00568	937	74	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	45037031	216130	0	3H-Histamine	-70	4	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@H](CC1=CN=CN1)N.Br.Br	None
	1223	950	41	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	950	41	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	950	41	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1204	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1919	119	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	2286	3161	51	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	4927	3161	51	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	7282	3161	51	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	CHEMBL643	3161	51	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	DB01069	3161	51	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	1204	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	3032915	15463	24	3H-Histamine	-1	7	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15463	24	3H-Histamine	-1	7	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	1204	1919	119	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1251	2001	39	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1251	2001	39	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1251	2001	39	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	2001	39	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	2001	39	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	2001	39	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	2001	39	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	2001	39	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	2001	39	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	None	216228	0	125I-iodophenpropit	-13	21	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	None	216228	0	3H-JNJ 7777120	-13	21	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	103	4122	61	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2875	4122	61	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	5736	4122	61	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL285802	4122	61	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB09225	4122	61	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	135398737	955	93	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	1204	1919	119	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	11957573	1997	34	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1997	34	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1997	34	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1997	34	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	277	1289	62	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1289	62	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1289	62	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1289	62	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1289	62	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1241	2024	52	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	1242	2024	52	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	3614	2024	52	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	CHEMBL507	2024	52	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	None	216115	0	3H-Histamine	-7585	7	Human	6.6	pKi	=	6.6	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	100	3776	58	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	2637	3776	58	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	5452	3776	58	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	CHEMBL479	3776	58	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	DB00679	3776	58	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	1204	1919	119	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1226	2006	20	3H-N-alpha-methylhistamine	2	7	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	41376	2006	20	3H-N-alpha-methylhistamine	2	7	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	CHEMBL12608	2006	20	3H-N-alpha-methylhistamine	2	7	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	45037031	216130	0	3H-N-alpha-methylhistamine	-70	4	Human	6.6	pKi	=	6.6	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@H](CC1=CN=CN1)N.Br.Br	None
	1267	3775	49	3H-Histamine	-33	28	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-Histamine	-33	28	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-Histamine	-33	28	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1210	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	1213	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2725	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	33036	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	4411	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	616	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	6976	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	716121	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	90475904	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1201353	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1554789	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL505	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB01114	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB13679	915	51	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	135398737	955	93	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	1239	2020	24	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	1240	2020	24	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	912	2020	24	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	CHEMBL268490	2020	24	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	180	400	56	3H-PYRILAMINE	-32	40	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	200	400	56	3H-PYRILAMINE	-32	40	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	2160	400	56	3H-PYRILAMINE	-32	40	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	CHEMBL629	400	56	3H-PYRILAMINE	-32	40	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	DB00321	400	56	3H-PYRILAMINE	-32	40	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	1204	1919	119	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	None	216115	0	3H-Histamine	-7585	7	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	1248	1412	28	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	3077	1412	28	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	90045	1412	28	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	CHEMBL12344	1412	28	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	11957573	1997	34	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1997	34	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1997	34	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1997	34	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	921599	19423	51	3H-Histamine	234	2	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	None
	CHEMBL129660	19423	51	3H-Histamine	234	2	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	None
	1204	1919	119	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1267	3775	49	125I-iodophenpropit	-33	28	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	125I-iodophenpropit	-33	28	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	125I-iodophenpropit	-33	28	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3032915	15463	24	3H-Histamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	3032915	15463	24	3H-N-alpha-methylhistamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15463	24	3H-Histamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15463	24	3H-N-alpha-methylhistamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	135398737	955	93	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	45073439	216131	0	3H-N-alpha-methylhistamine	-75	4	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@@H](CC1=CN=CN1)N.Br.Br	None
	1224	1420	83	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	3100	1420	83	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	8980	1420	83	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	916	1420	83	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	CHEMBL657	1420	83	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	DB01075	1420	83	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	2782	31970	67	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL1407943	31970	67	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL3216363	31970	67	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	1223	950	41	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	950	41	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	950	41	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	4761	13409	42	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1193	13409	42	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1580221	13409	42	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	1267	3775	49	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3775	49	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	115237	55555	119	None	-891	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	CHEMBL1621	55555	119	None	-891	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	2247	504	81	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	249	504	81	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	2603	504	81	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	CHEMBL296419	504	81	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	DB00637	504	81	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	5625	199645	72	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	456	6	3	6	2.7	CC(C)Nc1cccnc1N1CCN(C(=O)c2cc3cc(NS(C)(=O)=O)ccc3[nH]2)CC1	None
	CHEMBL593	199645	72	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	456	6	3	6	2.7	CC(C)Nc1cccnc1N1CCN(C(=O)c2cc3cc(NS(C)(=O)=O)ccc3[nH]2)CC1	None
	1613	2333	53	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	205	2333	53	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	3964	2333	53	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	CHEMBL831	2333	53	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	DB00408	2333	53	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	16106	118120	34	None	-20	12	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	None
	CHEMBL340801	118120	34	None	-20	12	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	None
	4761	13409	42	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1193	13409	42	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1580221	13409	42	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	135398737	955	93	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	100	3776	58	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	2637	3776	58	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	5452	3776	58	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	CHEMBL479	3776	58	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	DB00679	3776	58	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	16362	3103	71	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	2172	3103	71	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	90	3103	71	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	CHEMBL1423	3103	71	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	DB01100	3103	71	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	135398737	955	93	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	103	4122	61	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2875	4122	61	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	5736	4122	61	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL285802	4122	61	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB09225	4122	61	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2726	916	68	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	916	68	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	916	68	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	916	68	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	916	68	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	2726	916	68	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	916	68	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	916	68	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	916	68	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	916	68	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	1613	2333	53	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	205	2333	53	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	3964	2333	53	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	CHEMBL831	2333	53	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	DB00408	2333	53	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	135398737	955	93	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	11957573	1997	34	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1997	34	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1997	34	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1997	34	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	2782	31970	67	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL1407943	31970	67	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL3216363	31970	67	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	1210	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	1213	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2725	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	33036	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	4411	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	616	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	6976	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	716121	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	90475904	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1201353	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1554789	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL505	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB01114	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB13679	915	51	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2286	3161	51	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	4927	3161	51	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	7282	3161	51	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	CHEMBL643	3161	51	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	DB01069	3161	51	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	2726	916	68	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	916	68	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	916	68	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	916	68	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	916	68	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	1204	1919	119	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1919	119	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1223	950	41	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1223	950	41	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	950	41	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	950	41	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	950	41	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	950	41	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	135	2515	43	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1796	2515	43	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	4184	2515	43	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	CHEMBL6437	2515	43	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	DB06148	2515	43	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1267	3775	49	3H-JNJ 7777120	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3775	49	3H-JNJ-5207852	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-JNJ 7777120	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3775	49	3H-JNJ-5207852	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-JNJ 7777120	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3775	49	3H-JNJ-5207852	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1204	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1919	119	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	180	400	56	None	-32	40	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	200	400	56	None	-32	40	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	2160	400	56	None	-32	40	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	CHEMBL629	400	56	None	-32	40	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	DB00321	400	56	None	-32	40	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	277	1289	62	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1289	62	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1289	62	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1289	62	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1289	62	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1204	1919	119	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1919	119	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1919	119	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1919	119	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1919	119	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1919	119	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	135398737	955	93	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	135398737	955	93	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	135398737	955	93	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	955	93	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	955	93	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	955	93	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	955	93	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	115237	55555	119	3H-Histamine	-891	55	Human	5.1	pKi	=	5.1	Binding	NoneNone	PDSP KiDatabase	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	CHEMBL1621	55555	119	3H-Histamine	-891	55	Human	5.1	pKi	=	5.1	Binding	NoneNone	PDSP KiDatabase	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	1251	2001	39	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	2001	39	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	2001	39	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	23650961	3817	26	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	9276	3817	26	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	CHEMBL3301609	3817	26	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	DB12522	3817	26	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	1265	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	1265	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	1265	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	6422124	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	6422124	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	6422124	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	CHEMBL14638	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	CHEMBL14638	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	CHEMBL14638	939	60	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	1267	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	1267	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	1267	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	1267	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1267	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	1267	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	3035905	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	3035905	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	3035905	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	3035905	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	3035905	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	CHEMBL260374	3775	49	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	10446295	2119	34	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	1277	2119	34	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	CHEMBL185951	2119	34	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	1223	950	41	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	950	41	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	950	41	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1274	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	3063228	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	CHEMBL1196470	4033	16	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	1269	121	41	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	1269	121	41	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	37463	121	41	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	37463	121	41	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	CHEMBL275443	121	41	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	CHEMBL275443	121	41	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	1223	950	41	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	950	41	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	950	41	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1223	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	1223	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	1223	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1223	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	1223	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	2790	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	2790	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	2790	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	2790	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	CHEMBL14690	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	CHEMBL14690	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	CHEMBL14690	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	CHEMBL14690	950	41	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	24994634	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	24994634	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	8984	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	8984	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	CHEMBL3236549	2129	31	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	45257132	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	8983	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	CHEMBL595180	3656	10	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	1278	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1278	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	1278	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	1279	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1279	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	1279	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	4908365	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	4908365	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	4908365	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	CHEMBL129198	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	CHEMBL129198	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	CHEMBL129198	2137	84	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	117072554	2010	0	None	81	4	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	450	5	2	6	2.3	CN1CCN(CC1)C1=CC(=NC(N1)N)c1ccc2c(c1)CN(CC2)C(=O)CCC1CCCC1	26084539
	8982	2010	0	None	81	4	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	450	5	2	6	2.3	CN1CCN(CC1)C1=CC(=NC(N1)N)c1ccc2c(c1)CN(CC2)C(=O)CCC1CCCC1	26084539
	24745335	294	12	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	8985	294	12	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	CHEMBL1915540	294	12	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	DB15027	294	12	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	5048	3111	64	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	8924	3111	64	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	9948102	3111	64	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	CHEMBL462605	3111	64	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	DB11642	3111	64	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	441082	1154	48	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	8981	1154	48	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	CHEMBL191703	1154	48	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	11697697	2530	24	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	7346	2530	24	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	CHEMBL476323	2530	24	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	DB11910	2530	24	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	1547484	937	74	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	654	937	74	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	9072	937	74	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	CHEMBL43064	937	74	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	DB00568	937	74	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	10058807	2005	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	1276	2005	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	CHEMBL2261340	2005	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	135398737	955	93	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	955	93	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	955	93	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	955	93	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	955	93	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	1238	2069	13	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	6603865	2069	13	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	CHEMBL11919	2069	13	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	1248	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	1248	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	1248	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	1248	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	3077	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	3077	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	3077	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	3077	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	90045	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	90045	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	90045	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	90045	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	CHEMBL12344	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	CHEMBL12344	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	CHEMBL12344	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	CHEMBL12344	1412	28	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	135398737	955	93	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	955	93	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	955	93	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	955	93	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	955	93	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	11313837	2483	8	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	1254	2483	8	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	CHEMBL175782	2483	8	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	124846	1933	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	1273	1933	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	CHEMBL67601	1933	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	12656	2027	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	1270	2027	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	CHEMBL12704	2027	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	1194	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	23786499	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	CHEMBL26116	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	1229	740	0	None	-19	6	Rat	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	740	0	None	-19	6	Rat	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1229	740	0	None	-15	6	Mouse	6.1	pKi	None	6.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	740	0	None	-15	6	Mouse	6.1	pKi	None	6.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1236	2054	25	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2054	25	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2054	25	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2054	25	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1241	2024	52	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	2024	52	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	2024	52	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	2024	52	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1268	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	15947036
	1268	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	16432504
	92613	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	15947036
	92613	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	16432504
	1236	2054	25	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2054	25	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2054	25	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2054	25	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	135398737	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	135398737	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	135398737	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	135398737	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	38	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	38	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	38	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	722	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	722	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	722	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	CHEMBL42	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	CHEMBL42	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	CHEMBL42	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	DB00363	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	DB00363	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	DB00363	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	955	93	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	1241	2024	52	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	2024	52	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	2024	52	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	2024	52	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1236	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1236	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	1236	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	1236	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1237	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	1237	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	1237	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	156615	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	156615	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	156615	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	CHEMBL268229	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	CHEMBL268229	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	CHEMBL268229	2054	25	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1271	2023	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	44292270	2023	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	CHEMBL46296	2023	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	1239	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	1239	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	1240	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	1240	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	912	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	912	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	CHEMBL268490	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	CHEMBL268490	2020	24	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	1229	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179434
	1229	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179436
	1229	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11181941
	1229	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1229	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	15947036
	5311293	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179434
	5311293	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179436
	5311293	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11181941
	5311293	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	740	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	15947036
	1204	1919	119	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1919	119	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1919	119	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1919	119	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1919	119	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1919	119	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1919	119	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1919	119	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1919	119	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1919	119	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1919	119	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1919	119	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1241	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1241	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1241	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	1242	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1242	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	3614	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	3614	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	CHEMBL507	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	CHEMBL507	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	2024	52	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	1267	3775	49	None	-21	28	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1267	3775	49	None	-14	28	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3775	49	None	-21	28	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3775	49	None	-14	28	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3775	49	None	-21	28	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3775	49	None	-14	28	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1266	2044	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	3960	2044	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	5311186	2044	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	1226	2006	20	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	1226	2006	20	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	41376	2006	20	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	41376	2006	20	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	CHEMBL12608	2006	20	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	CHEMBL12608	2006	20	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	1251	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	1251	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	1251	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	3035842	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	3035842	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	3035842	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	CHEMBL18661	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	CHEMBL18661	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	CHEMBL18661	2001	39	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1204	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1247	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1375	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	774	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	CHEMBL90	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	DB05381	1919	119	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	11957573	1997	34	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	11957573	1997	34	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1997	34	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1997	34	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1997	34	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1997	34	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1997	34	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1997	34	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	11957573	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	11957573	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	11957573	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	11957573	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	1250	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	1250	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	1250	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	3692	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	3692	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	3692	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	CHEMBL19439	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	CHEMBL19439	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	CHEMBL19439	1997	34	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071

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